Primary orthostatic tremor in Great Danes

Two Great Danes were presented for an investigation of 6-month histories of incessant tremor of all 4 limbs and trunk occurring only when the dogs were standing at rest. Dog 1 was a 2-year-old intact female harlequin Great Dane living in the UK, and dog 2 was a 2-year-old castrated male fawn Great Dane living in the United States. The owners described both dogs as having constant quivering of the limbs while standing, suggestive of an involuntary dance, since the age of 18 months. The intensity of the tremors was reported in both dogs to have steadily increased since onset. In dog 1, tremors were noticed to be more pronounced in the fore limbs when she was eating and more pronounced in the hind limbs when she was trying to sit. The owner of dog 1 also reported that the dog could no longer assume a position of recumbency without manual assistance because of the severity of the tremors. Intermittently, tremors were reported to involve the facial musculature of dog 2. Vaccinations were current, and the owners of both dogs did not report any previous medical history, exposure to natural or manufactured toxins, or concurrent treatment. No familial relationship was identified between these 2 dogs.     

Genetic structure and diversity among sheep breeds in the United States: identification of the major gene pools

Understanding existing levels of genetic diversity of sheep breeds facilitates in situ and ex situ conservation activities. A comprehensive evaluation of US sheep breeds has not been previously performed; therefore, we evaluated the genetic diversity among and within 28 US sheep breeds. Both major and minor breeds were included in the analysis and consisted of 666 animals from 222 producers located in 38 states. The level of within-breed genetic diversity was variable and not dependent upon status of a breed as a major or minor breed. Bayesian cluster analysis indicated the breeds were grouped more by physiological differences (meat vs. wool production) rather than geographic origin. Results suggest several actionable items to improve in situ and ex situ conservation. The results clearly identify breeds in need of increased in situ and ex situ management (e.g., Hog Island and Karakul) and allow several suggestions for in situ management of flocks. Conversely, several of the breeds appear genetically similar and therefore require less emphasis on collecting germplasm samples for the gene bank. Commercially important breeds (e.g., Rambouillet and Suffolk) were found to have substantial variation, which should enable breeders to proceed, unencumbered by genetic diversity concerns, with selection strategies that maximize profit.     

Dose-titration studies of ivermectin against experimental Ancylostoma caninum and Uncinaria stenocephala infections

Dose-titration trials of ivermectin were conducted on pups with dual experimental infections of 4th-stage larvae or adult Ancylostoma caninum and Uncinaria stenocephala. Ivermectin was administered orally or subcutaneously at dosages of 0.006, 0.012, or 0.024 mg/kg of body weight. Maximal or near maximal (greater than or equal to 96% to 100%) anthelmintic effect was observed for both stages of development for each hookworm species by either route of administration at a dosage of 0.024 mg/kg. Responses for all of the aforementioned categories were linearly related to increasing log dosage of ivermectin, with common slopes (regression coefficients). Regression analysis also provided estimates of the minimal dosages required to produce maximal reduction in worm burden for each stage, species, and route of administration. The estimated ivermectin dosages for maximal efficacy ranged from a low of 0.014 mg/kg for adult A caninum by oral treatment to 0.044 mg/kg for 4th-stage larvae of U stenocephala by oral treatment.