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51.
W. Lilenbaum J. C. Schettini G. N. Souza E. R. Ribeiro E. C. Moreira L. S. Fonseca 《Zoonoses and public health》1999,46(5):353-358
Bovine tuberculosis is a major problem in Brazil. The intradermal tuberculin test is the standard test for detection of bovine tuberculosis in Brazil but can lack both sensitivity and specificity. The purpose of this study was to compare a bovine γ-IFN assay with the tuberculin test under field conditions in Brazil. A total of 1632 animals from 13 dairy farms were tested using the single cervical tuberculin test (SCTT). Among those animals, about 15% of each herd, 220 in total, represented a high-risk group and were selected to be tested using the γ-IFN test. Of the 1632 animals tested, 207 presented significant reactions representing 12.7% of the cattle studied. In the selected group the number of animals positive by the γ-IFN assay was 126/220 (57.3%) and the total number of reactive cows on SCTT was 106/220 (48.2%). The real number of infected cattle, following standards, was 120/220 (54.5%). From these results the relative sensitivity rate of γ-IFN test was 100% including the false-positive results and 88.3% for the SCTT — a significant (P < 0.01) difference in favour of the γ-IFN test of 11.7%. The γ-IFN assay also identified some positive animals 60–120 days earlier than the SCTT. In conclusion, we believe that the γ-IFN assay can be used alone or in combination with the SCTT, as a valuable tool for the control of bovine tuberculosis in the Brazilian national herd. 相似文献
52.
53.
Érika Ramos de Alvarenga Suellen Cristina Moreira de Sales Túlio Soares de Brito Cláudia Regina Santos Rebeca Dias Serafim Corrêa Gabriel Francisco de Oliveira Alves Ludson Guimarães Manduca Eduardo Maldonado Turra 《Aquaculture Research》2017,48(12):5965-5972
The objectives of this study were to evaluate the reproductive cycle, morphological changes of ovary and mobilization of energy reserves in Nile tilapia reared with biofloc technology (BFT). In general, the growth and reproductive performance were highly similar between BFT and Control system (clear water). Difference between the systems was found in the hepatosomatic index (using mixed‐effects models), which suggested that BFT can alter the energy mobilization in the post‐spawning period. The absolute and relative fecundity, fertilization rate, number of larvae produced per female, gonadosomatic index, proportion of oogenesis cells, number of post‐ovulatory and atretic follicles were similar between the two systems. We also did not detect a reduction in the reproductive cycle length in Nile tilapia reared in BFT. Because there was no evidence of the negative effects of BFT on Nile tilapia reproduction, we concluded that BFT might be used for breeder stocking of this species. 相似文献
54.
Ana Lilia Alzate-Marin Marcia Regina Costa Klever Marcio Arruda Everaldo Gonçalves de Barros Maurilio Alves Moreira 《Euphytica》2003,133(2):165-169
Ouro Negro (Honduras 35) is a highly productive Mesoamerican black seeded bean cultivar that possesses a major dominant gene
conferring resistance to anthracnose (causal organism Colletotrichum lindemuthianum). In this work the anthracnose resistance gene present in Ouro Negro was characterized by studying allelic relationships
to the following previously characterized anthracnose resistance genes (cultivars): Co-1 (MDRK), Co-1
2 (Kaboon), Co-1
3 (Perry Marrow), Co-2 (Cornell 49-242), Co-3 (Mexico 222), Co-4 (TO), Co-4
2 (SEL 1308), Co-5 (SEL1360), Co-6 (AB 136), and the resistance genes present in PI 207262 and Widusa. In addition, we determined the resistance spectrum of
Ouro Negro in relation to 19 pathotypes of C. lindemuthianum. The allelism tests confirmed that the dominant anthracnose resistance gene present in Ouro Negro is positioned at a locus
distinct from those with which it was compared. We propose that this new gene be named Co-10. The inoculation of Ouro Negro with the 19 pathotypes of C. lindemuthianum demonstrated that Co-10 confers resistance to pathotypes 23, 64, 67, 73, 81, 83, 87, 89, 95, 102, 117, 119, 343, 453, 1033, 1545 and 1600. The identification
of Co-10 is an important contribution to bean breeding programs that are in constant need of new sources of resistance to anthracnose.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
55.
González-Wangüemert Mercedes Roggatz Christina C. Rodrigues Maria João Barreira Luísa da Silva Manuela Moreira Custódio Luísa 《International Aquatic Research》2018,10(4):361-373
International Aquatic Research - This work makes a comparative evaluation of the biochemical profile of three sea commercial cucumber species (Holothuria mammata, H. polii and H. tubulosa) caught... 相似文献
56.
Involvement of cholinergic and adenosinergic systems on the branchial immune response of experimentally infected silver catfish with Streptococcus agalactiae
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M D Baldissera C F Souza P H Doleski K L S Moreira M L da Veiga M I U M da Rocha R C V Santos B Baldisserotto 《Journal of fish diseases》2018,41(1):27-32
It has been recognized that the cholinergic and adenosinergic systems have an essential role in immune and inflammatory responses during bacterial fish pathogens, such as the enzymes acetylcholinesterase (AChE) and adenosine deaminase (ADA), which are responsible for catalysis of the anti‐inflammatory molecules acetylcholine (ACh) and adenosine (Ado) respectively. Thus, the aim of this study was to investigate the involvement of the cholinergic and adenosinergic systems on the immune response and inflammatory process in gills of experimentally infected Rhamdia quelen with Streptococcus agalactiae. Acetylcholinesterase activity decreased, while ACh levels increased in gills of infected animals compared to uninfected animals. On the other hand, a significant increase in ADA activity with a concomitant decrease in Ado levels was observed in infected animals compared to uninfected animals. Based on this evidence, we concluded that infection by S. agalactiae in silver catfish alters the cholinergic and adenosinergic systems, suggesting the involvement of AChE and ADA activities on immune and inflammatory responses, regulating the ACh and Ado levels. In summary, the downregulation of AChE activity exerts an anti‐inflammatory profile in an attempt to reduce or prevent the tissue damage, while the upregulation of ADA activity exerts a pro‐inflammatory profile, contributing to disease pathophysiology. 相似文献
57.
First feeding of diploid and triploid yellowtail tetra Astyanax altiparanae: An initial stage for application in laboratory studies
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Rafaela Manchin Bertolini José Augusto Senhorini Nivaldo Ferreira do Nascimento Matheus Pereira‐Santos Laura Satiko Okada Nakaghi Wellington Adriano Moreira Peres Regiane Cristina da Silva George Shigueki Yasui 《Aquaculture Research》2018,49(1):68-74
In this study, the aim was to establish a protocol for first feeding of diploid and triploid yellowtail tetra Astyanax altiparanae in laboratory conditions. The fry were fed with five different diets: (i) Artemia franciscana nauplii, (ii) plankton, (iii) dry food, (iv) Artemia franciscana nauplii + plankton, and (v) Artemia nauplii + plankton + dry food. Additionally, the growth and survival rates of diploid and triploid individuals were also evaluated. On day 10, the length of the fish between the treatments differed significantly (p = .0001) and ranged from 4.07 ± 0.06 mm (dry food) to 8.50 ± 0.64 mm (plankton + Artemia). The sizes of the fish increased with time, except for the fish fed with dry food. The survival rates were similar for the fish fed with the four diets and ranged from 80.7 ± 5.4% (dry food + plankton + Artemia to 92.0 ± 1.6% (plankton + Artemia), but differed from the fish fed with dry food (17.7 ± 5.8%, p = .0017). Diploids and triploids did not present differences on day 0 (p = .2252) and on day 10 (p = .4844) when the fish presented 6.77 ± 0.25 mm and 6.54 ± 0.15 mm respectively. Survival of diploids (87.3 ± 5.13%) and triploids (74.67 ± 2.30%) were also similar (p = .0285). These data are innovative and useful for establishing protocols for this species in both academic and applied sciences. 相似文献
58.
José Maria Villela Pádua Kaio Olímpio Das Graças Dias Maria Marta Pastina João Cândido de Souza Valéria Aparecida Vieira Queiroz Rodrigo Veras da Costa Maria Beatriz Pereira da Silva Carlos Alexandre Gomes Ribeiro Claudia Teixeira Guimarães Salvador Alejandro Gezan Lauro José Moreira Guimarães 《Euphytica》2016,211(3):277-285
59.
60.
Diego Moreira de Souza Sabrina Medeiros Suita Luis Alberto Romano Wilson Wasielesky Jr Eduardo Luis Cupertino Ballester 《Aquaculture Research》2014,45(2):270-277
The present work evaluated the use of molasses as a carbon source during the nursery rearing of Farfantepenaeus brasiliensis in a Biofloc technology system. During a 30 days trial, three replicate tanks were randomly assigned to the following treatments: 1.) molasses (with molasses addition) and 2.) control (without molasses addition). Bacteriological analysis was used to quantify the abundance of presumptive Vibrio spp. between control and molasses treatment. The concentration of this microorganism was lower in molasses compared with control. For the immunological analysis, shrimp haemolymph was collected to determine the total haemocyte count and the total protein concentration. The immunological results were not different between treatments. The performance results of shrimp reared with molasses addition showed that the survival rate (88.87 ± 6.36), the mean final weight (1.22 ± 0.38) and the specific growth rate (0.0309 ± 1.06) were significantly higher compared with control (80.5 ± 2.42; 1.03 ± 0.13; 0.0256 ± 0.97 respectively). Moreover, the addition of molasses contributed to the maintenance of water quality and lower concentration of presumptive Vibrio spp. The control presented an unstable variation of Vibrio spp. reaching values of 80 × 102 CFU/ml, while the highest result of molasses was 20 × 102 CFU/ml, confirming the beneficial effects of molasses addition. 相似文献