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81.
Tsuyoshi Ohira Katsuyoshi Suitoh Fumihiro Yamane Chiaki Nagai Michio Suzuki Naoaki Tsutsui Hiromichi Nagasawa Susumu Izumi 《Fisheries Science》2010,76(4):605-611
Crustacean hyperglycemic hormone (CHH) is released from the X-organ/sinus gland complex located in the eyestalks. In this
study, the most abundant CHH in the sinus gland of the greasyback shrimp Metapenaeus ensis was purified by reversed-phase HPLC and identified by N-terminal amino acid sequencing. Although two CHH molecules (Mee-CHH-A
and Mee-CHH-B) have already been identified from M. ensis by cDNA cloning, this study revealed the presence of an additional CHH peptide based on differences in the N-terminal amino
acid sequences of the CHH-A and CHH-B. Therefore, this novel CHH was designated as Mee-CHH-C. A cDNA encoding the Mee-CHH-C
precursor was cloned by RT-PCR coupled with 5′- and 3′-RACE, and it was found that the mature Mee-CHH-C consisted of 72 amino
acid residues containing 6 conserved cysteine residues and possessed an amidated C terminus. Mee-CHH-C had 62 and 68% identities
with Mee-CHH-A and Mee-CHH-B, respectively, and was highly homologous to CHHs characterized from other penaeid shrimp species.
The hyperglycemic activity of Mee-CHH-C was examined by an in vivo bioassay using the kuruma prawn Marsupenaeus japonicus. Injection of Mee-CHH-C increased hemolymph glucose levels significantly and dose-dependently. These results indicate that
Mee-CHH-C is possibly one of the major molecules in M. ensis that regulate glucose levels in the hemolymph. 相似文献
82.
Junpei Shinji Bong Jung Kang Tomoyuki Okutsu Kota Banzai Tsuyoshi Ohira Naoaki Tsutsui Marcy N. Wilder 《Fisheries Science》2012,78(4):833-840
Changes in crustacean hyperglycemic hormone (CHH)-family peptides in response to stress were investigated in Litopenaeus vannamei. Stress treatments consisted of air exposure and low salinity. High-performance liquid chromatography was used to quantify CHH-family peptides in the X-organ?Csinus gland complex (XO?CSG) in the eyestalks. Among the CHH-family peptides analyzed, only the level of sinus gland peptide-G (SGP-G) in the XO?CSG was decreased. SGP-G was also detectable by Western blotting analysis in the hemolymph of animals subjected to stress. These results suggest that SGP-G was secreted from the XO?CSG into the hemolymph during stress. Glucose levels in the hemolymph increased under conditions during which SGP-G was detected in the hemolymph. Hyperglycemia was also observed when SGP-G was injected. SGP-G may function to shift energy use to deal with stress. 相似文献
83.
84.
During an asexual growth cycle of Babesia parasites in a natural host, the extracellular merozoites invade (i.e., attach to, penetrate, and internalize) the host erythrocytes (RBC) via multiple adhesive interactions of several protozoan ligands with the target receptors on the host cell surface. After internalizing the host RBC, they asexually multiply, egress from the RBC by rupturing the host cells, and then invade the new RBC again. In the invasion stage, several surface-coating molecules of merozoites might be involved in the initial attachment to the RBC, while proteins secreted from apical organelles (rhoptry, microneme, and spherical body) are proposed to play roles mainly in erythrocyte penetration or internalization. On the other hand, several components located on the surface of the RBC, such as sialic acid residues, protease-sensitive proteins, or sulphated glycosaminoglycans, are identified or suspected as the host receptors of erythrocyte invasion by Babesia parasites. The detailed molecular interactions between Babesia merozoites and the host RBC are incompletely understood. In this review, these identified or suspected molecules (protozoan ligands/erythrocyte receptors) are described by especially focusing on Babesia bovis. 相似文献
85.
Onda K Matsuki N Ono K Wada Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(1):9-13
The expression of parathyroid hormone-related protein (PTHrP) mRNA was examined in mammary gland with or without lactation, and during periparturient period in a Holstein cow and a Jersey cow. In the lactating mammary gland, PTHrP was detected in alveolar epithelial cells and the lumen by immunohistochemical analysis. The relative expression levels of PTHrP mRNA in mammary gland from lactating cows were significantly higher than those from non-lactating cows (P<0.05). During periparturient period, relative PTHrP mRNA level was remarkably low before the parturition in a Jersey and a Holstein cow, however, both levels were gradually increased and reached a peak level at 5-6 weeks after the parturition. In addition, the peak level in a Jersey cow was approximately 3-fold higher than that in a Holstein cow. From these results, PTHrP was synthesized and secreted in alveolar epithelial cells in mammary gland and increased subsequently with the lactation, suggesting a possible mechanism for the regulation of local calcium homeostasis. 相似文献
86.
Takiyama N Shoji S Habata I Ohba S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(6):631-633
The effects of a timolol maleate gel-forming solution (TMGS) on intraocular pressure (IOP), blood pressure (BP), and pupil size (PS) were evaluated in normotensive dogs. TMGS was administered once daily to six normotensive beagle dogs. TMGS administration reduced IOP and PS. The hypotensive effect persisted for 24 hr after the administration. The mean reduction in IOP was 5.3 mm Hg (P<0.01). The changes in BP and PS were not significant. These results suggest that TMGS can potentially be used in the treatments of glaucoma and ocular hypertension in dogs. 相似文献
87.
Terkawi MA Alhasan H Huyen NX Sabagh A Awier K Cao S Goo YK Aboge G Yokoyama N Nishikawa Y Kalb-Allouz AK Tabbaa D Igarashi I Xuan X 《Veterinary parasitology》2012,187(1-2):307-311
A total of 207 bovine blood samples were collected from clinically healthy cattle bred in central region of Syria and examined by Giemsa-stained blood smears, nested PCR, ELISA, and IFAT to determine the molecular and serological prevalence of Babesia bovis and B. bigemina. All samples were negative to Babesia spp. by microscopic examination of blood smears. On the other hand, the overall prevalence of B. bovis and B. bigemina was 9.18% and 15.46% by nPCR, 15.46% and 18.84% by ELISA, and 18.36% and 21.74% by IFAT, respectively. Mixed infections were detected in a total of 5 samples (2.4%) by nPCR, 16 (7.73%) by ELISA and 27 (13.04%) by IFAT. Statistically significant differences in the prevalence of the two infections were observed on the basis of age and location. These data provide valuable information regarding the occurrence and epidemiology of B. bovis and B. bigemina infections in Syrian cattle, which can be employed in developing rational strategies for disease control and management. 相似文献
88.
Kenjiro FUKUSHIMA Reina FUJIWARA Kie YAMAMOTO Hideyuki KANEMOTO Koichi OHNO Masaya TSUBOI Kazuyuki UCHIDA Naoaki MATSUKI Ryohei NISHIMURA Hajime TSUJIMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1549-1553
Little information is available regarding triple-phase computed tomography (CT) of canine
pancreatic insulinoma. A few case reports with small numbers of cases have indicated that
hyper-attenuation in the arterial phase was a common finding on multi-phasic CT in dogs
with insulinoma. Our purpose was to clarify the characteristic findings of dogs with
insulinoma on triple-phase CT. Nine dogs with insulinoma that underwent triple-phase CT
were included in the present study. Attenuation patterns in the arterial phase indicated
hypo-attenuation in 4 cases and hyper-attenuation in 2 cases. In the remaining 3 cases, 1
case showed hypo-attenuation and 1 case showed hyper-attenuation in the pancreatic phase,
and 1 case presented hyper-attenuation in the later phase. Altogether, 5 cases showed hypo
and 4 cases showed hyper-attenuation in at least one phase. The enhancement pattern was
homogenous in 7 cases and heterogeneous in 2 cases. Tumor margins were well-defined in 5
cases and ill-defined in 4 cases. Capsule formation was present in 5 cases and absent in 4
cases. In conclusion, it is important to note that hypo-attenuation was as common as
hyper-attenuation in dogs with insulinoma in triple-phase CT in at least one phase.
Additionally, mass lesions were most conspicuous not only in the arterial phase but in the
pancreatic and later phases in some cases. Therefore, it is important to perform
triple-phase CT and notice about variable findings for the detection of canine pancreatic
insulinoma. 相似文献
89.
Marine bacteria have not been examined as extensively as land bacteria. We screened carotenoids from orange or red pigments-producing marine bacteria belonging to rare or novel species. The new acyclic carotenoids with a C30 aglycone, diapolycopenedioc acid xylosylesters A–C and methyl 5-glucosyl-5,6-dihydro-apo-4,4′-lycopenoate, were isolated from the novel Gram-negative bacterium Rubritalea squalenifaciens, which belongs to phylum Verrucomicrobia, as well as the low-GC Gram-positive bacterium Planococcus maritimus strain iso-3 belonging to the class Bacilli, phylum Firmicutes, respectively. The rare monocyclic C40 carotenoids, (3R)-saproxanthin and (3R,2′S)-myxol, were isolated from novel species of Gram-negative bacteria belonging to the family Flavobacteriaceae, phylum Bacteroidetes. In this review, we report the structures and antioxidant activities of these carotenoids, and consider relationships between bacterial phyla and carotenoid structures. 相似文献
90.
Effects of physical and chemical factors on differentiation and growth of Begonia × hiemalis Fotsch, cultivar ‘Schwabenland Red’, in vitro were examined, and a mass-propagation scheme was established. In shaking-culture, differentiated buds grew rapidly into large aggregates of plantlets without roots. They were divided into plantlets and then transferred to an agar medium for rooting. Theoretically, by this method 1014 or more plantlets may be produced in one year from a 7 × 7-mm young leaf segment. 相似文献