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21.
Hepatitis E virus infection dynamics and organic distribution in naturally infected pigs in a farrow-to-finish farm 总被引:1,自引:0,他引:1
de Deus N Casas M Peralta B Nofrarías M Pina S Martín M Segalés J 《Veterinary microbiology》2008,132(1-2):19-28
The objective of the present study was to determine the pattern of Hepatitis E virus (HEV) infection in a naturally infected, farrow-to-finish herd. For that purpose, a prospective study was conducted in randomly selected 19 sows and 45 piglets. Blood samples were collected from sows at 1 week post-farrowing and from piglets at 1, 3, 6, 9, 12, 15, 18 and 22 weeks of age. Furthermore 3 or 5 animals were necropsied at each bleeding day (but at 1 week of age), and serum, bile, liver, mesenteric lymph nodes and faeces taken. HEV IgG, IgM and IgA antibodies were determined in serum and viral RNA was analysed in all collected samples by semi-nested RT-PCR. Histopathological examination of mesenteric lymph nodes and liver was also conducted. From 13 analysed sows, 10 (76.9%) were positive to IgG, one to IgA (7.7%) and two to IgM (15.4%) antibodies specific to HEV. In piglets, IgG and IgA maternal antibodies lasted until 9 and 3 weeks of age, respectively. IgG seroconversion occurred by 15 weeks of age while IgM and IgA at 12. On individual basis, IgG was detectable until the end of the study while IgM and IgA antibody duration was of 4-7 weeks. HEV RNA was detected in serum at all analysed ages with the highest prevalence at 15 weeks of age. HEV was detected in faeces and lymph nodes for the first time at 9 weeks of age and peaked at 12 and 15 weeks of age. This peak coincided with the occurrence of hepatitis as well as with HEV detection in bile, liver, mesenteric lymph nodes and faeces, and also with highest IgG and IgM OD values at 15 weeks. Finally, different HEV sequences from this farm were obtained, which they clustered within 3 different groups, together with other Spanish sequences, all of them of genotype 3. Moreover, the present study also indicates that the same pig can be infected with at least two different strains of HEV during its productive life. This is the first study characterizing HEV infection in naturally infected pigs with chronological virus detection and its relationship with tissue lesions throughout the productive life of the animals. 相似文献
22.
Segalés J Piñeiro C Lampreave F Nofrarías M Mateu E Calsamiglia M Andrés M Morales J Piñeiro M Domingo M 《Veterinary research》2004,35(3):275-282
The objective of this study was to determine the serum concentration levels of selected acute phase proteins (APP), haptoglobin (HPT) and pig-major acute phase protein (pig-MAP), in postweaning multisystemic wasting syndrome (PMWS) affected pigs and PCV2-subclinically infected pigs. In a first study, a group of 15 eight-week-old conventional pigs from a PMWS affected farm were bled and a complete necropsy, histopathology and in situ hybridisation to detect PCV2 were performed. Based on the results, pigs were classified as suffering from PMWS (n = 10) or healthy animals (n = 5). In a second study, a group of 45 pigs from another PMWS affected farm were selected and bled at 3, 7, 12 and 28 weeks of age. The assessment of PCV2 infection status in these pigs was retrospectively done by PCV2 PCR in serum samples. Selected APP were measured in the serum of all studied pigs by means of radial immunodiffusion. Mean HPT and pig-MAP levels were significantly increased (p = 0.004 and p = 0.0006 respectively) in PMWS-affected pigs when compared to levels found in healthy pigs (2.52 +/- 0.88 mg/mL vs. 1.06 +/- 0.73 mg/mL for HPT and 3.81 +/- 1.53 mg/mL vs. 0.76 +/- 0.34 mg/mL for pig-MAP). In the second study, no significant difference in mean HPT and pig-MAP values were observed between PCV2 PCR positive and negative pigs of any age. However, both APP increased significantly with age in PCV2 PCR negative pigs. Altogether, the present results suggest that APP levels are significantly increased in pigs that develop PMWS, but not in animals with a PCV2 subclinical infection. 相似文献
23.
Carneiro Mario Davi Dias Maltez Lucas Campos Rodrigues Ricardo Vieira Planas Miquel Sampaio Luís André 《Fish physiology and biochemistry》2021,47(4):841-848
Fish Physiology and Biochemistry - The nitrification process in recirculating aquaculture systems can reduce water pH. Fish can also be exposed to water acidification during transport, an important... 相似文献
24.
Leander Höhne Miquel Palmer Christopher T. Monk Sven Matern Robert Nikolaus Ashley Trudeau Robert Arlinghaus 《Ecology of Freshwater Fish》2020,29(4):557-573
Growth of fish is an important contributor to individual fitness as well as fish production. Explaining and predicting growth variation across populations is thus important from fundamental and applied perspectives, which requires knowledge about the ecological factors involved in shaping growth. To that end, we estimated environment-dependent von Bertalanffy growth models for 13 gravel pit lake populations of Eurasian perch (Perca fluviatilis) from north-western Germany. To identify the main drivers of perch growth, we evaluated the performance of 16 different biotic or abiotic lake variables in explaining growth variation among lakes. In addition, we compared growth predictions from the best-performing model incorporating “complex” variables that require intensive sampling effort, with a model using only “simple”, easily measurable lake variables (e.g. shoreline development factor). The derivation of a simple model aimed at future applications in typically data-poor inland fisheries, predicting expected growth potential from easily measurable lake variables. A model combining metabolic biomass of predators, maximum depth and shoreline development factor performed best in predicting perch growth variation across gravel pits. All three parameters in this model were positively related to perch growth. The best-performing simple model consisted only of the shoreline development factor. Length-at-age predictions from both models were largely identical, highlighting the utility of shoreline development factor in approximating growth potential of perch in gravel pits similar to our study lakes. Our results can be used to inform fisheries management and restoration efforts at existing or newly excavated gravel pit lakes. 相似文献
25.
Tao F Grass ME Zhang Y Butcher DR Renzas JR Liu Z Chung JY Mun BS Salmeron M Somorjai GA 《Science (New York, N.Y.)》2008,322(5903):932-934
Heterogeneous catalysts that contain bimetallic nanoparticles may undergo segregation of the metals, driven by oxidizing and reducing environments. The structure and composition of core-shell Rh(0.5)Pd(0.5) and Pt(0.5)Pd(0.5) nanoparticle catalysts were studied in situ, during oxidizing, reducing, and catalytic reactions involving NO, O2, CO, and H2 by x-ray photoelectron spectroscopy at near-ambient pressure. The Rh(0.5)Pd(0.5) nanoparticles underwent dramatic and reversible changes in composition and chemical state in response to oxidizing or reducing conditions. In contrast, no substantial segregation of Pd or Pt atoms was found in Pt(0.5)Pd(0.5) nanoparticles. The different behaviors in restructuring and chemical response of Rh(0.5)Pd(0.5) and Pt(0.5)Pd(0.5) nanoparticle catalysts under the same reaction conditions illustrates the flexibility and tunability of the structure of bimetallic nanoparticle catalysts during catalytic reactions. 相似文献
26.
Castillo M Martín-Orúe SM Nofrarías M Manzanilla EG Gasa J 《Veterinary microbiology》2007,124(3-4):239-247
An experiment was designed to monitor the changes in caecal microbiota associated with early weaning. Twelve piglets (20+/-2 days) from six different litters were selected from a commercial source. For the two experimental groups, one animal from each litter was weaned onto a post-weaning diet (W) and the other remained with the sow (S). After 1 week, animals were sacrificed and caecal samples taken. Microbial counts for total bacteria, enterobacteria and lactobacilli populations were determined by quantitative PCR using SYBR Green dye. Microbial profiles were assessed by terminal restriction fragment length polymorphism (t-RFLP). Weaning promoted an increase in the enterobacteria:lactobacilli ratio (0.27 versus 1.67 log/log 16S rRNA gene copy number, P=0.05). Total bacteria and richness of the caecal microbial ecosystem (number of peaks) were similar in both experimental groups (49.3 for S and 53.4 for W, respectively, P=0.22), although the band patterns were clearly grouped in two different clusters by dendogram analysis. Weaning was also associated with a decrease in crypt density, an increase in mytotic index and a decrease in the number of goblet cells. A reduced immunological response was also observed and was manifested by an increase in intraepithelial lymphocytes and lymphocyte density in the lamina propria. Weaning appears to be critical in the establishment of the caecal microbiota in pigs with important changes, particularly in microbial groups and in caecal mucosal architecture. 相似文献
27.
Xifré-Salvadó Miquel Àngel Prat-Guitart Núria Francos Marcos Úbeda Xavier Castellnou Marc 《European Journal of Forest Research》2022,141(5):753-767
European Journal of Forest Research - This study was conducted following the fires that took place in Rocallaura (Spain) between 23/06/2016 and 19/07/2017. The aim is to analyse the importance of... 相似文献
28.
Thymolipomas are rare benign thymic neoplasms recognised in humans, composed of mature adipose tissue and thymic tissue. An 8-year-old male domestic shorthair cat presented with anorexia, lethargy and vomiting secondary to the development of a thoracic effusion and an anterior mediastinal tumoral mass with fatty appearance. Histologically, the mass consisted of adipose tissue containing numerous cords and nests of cortical and medullary thymic tissue. No signs of thymic epithelial or lymphoid neoplastic proliferation were detected. The findings in this case are remarkably similar to the macroscopic and histological features of thymolipoma in humans. 相似文献
29.
Lecina-Diaz Judit Alvarez Albert De Cáceres Miquel Herrando Sergi Vayreda Jordi Retana Javier 《Landscape Ecology》2019,34(10):2307-2321
Landscape Ecology - Protected areas (PAs) are essential for biodiversity conservation and the provision of ecosystem services (ES), representing 15% of the earth’s surface and targeted to... 相似文献