树干液流及其主要影响因子对摘芽强度的响应

为了探讨摘芽强度对树木液流变化特征及其主要环境调控因子的影响,为人工林及果园等高效抚育管理提供参考,该研究以毛白杨(Populus tomentosa)为研究对象,设置4种摘芽处理(不摘芽CK、轻度摘芽BP1.0、中度摘芽BP1.5、重度摘芽BP2.0),采用热扩散式液流检测技术,结合自动气象站,对造林后第2～3个生长...     

甘露糖—小清蛋白美拉德反应产物的免疫活性

为探讨以甘露糖为还原糖进行美拉德反应对小清蛋白免疫活性的影响,通过将鲢重组小清蛋白(r PV)和甘露糖混合物在干热条件下(100°C)反应100 min制得糖化的r PV(Mr PV),采用Tricine-SDS-PAGE和斑点杂交(Dot-blotting)分析糖化前后r PV的聚合特性、免疫活性及消化稳定性的变化;采用圆二色谱仪和扫描电子显微镜分析糖化对r PV的二级结构及微观结构的影响。结果显示,r PV糖化后形成大分子量的片状聚集体;甘露糖糖化修饰的r PV免疫活性及消化稳定性均显著下降;美拉德反应导致r PV二级结构中的α-螺旋和无规则卷曲的含量有一定程度的减少,而β-折叠的含量显著增加。研究表明,以甘露糖为还原糖进行美拉德反应可有效降低r PV的免疫活性及消化稳定性,这可能与糖化后r PV聚集体的形成及二级结构的改变有关。     

饲料溶菌酶添加水平对氨氮应激下吉富罗非鱼血清生化指标、抗菌性能和肝脏抗氧化能力的影响

为研究氨氮应激下溶菌酶对吉富罗非鱼血清生化指标、抗菌性能及肝脏抗氧化能力的影响,用6种不同溶菌酶添加水平(0、18、36、54、72和90 mg/kg)的饲料(分别记为L0、L18、L36、L54、L72和L90)饲喂初始均重为(11.35±0.08)g的罗非鱼60 d,之后应用氯化铵进行24 h氨氮应激实验。结果显示:1应激后,各组鱼的血清生化指标在组间存在较大差异(P0.05),不同溶菌酶添加水平下的鱼体对氨氮应激产生了不同的响应机制。L54组鱼主要通过激发免疫系统并调节蛋白质代谢来抵抗氨氮浓度突变;L72和L90组鱼主要通过调节高密度脂蛋白和低密度脂蛋白、胆固醇和甘油三酯之间的动态变化来缓解氨氮对机体的应激。2血清抗菌实验表明,L54和L72组鱼对大肠杆菌、金黄色葡萄球菌和嗜水气单胞菌的抑制能力最大,且显著高于对照组(P0.05);L36~L90组对溶藻弧菌的抑制作用显著高于对照组和L18组(P0.05);各溶菌酶添加组的鱼对枯草芽孢杆菌具有不同程度的保护作用。3应激后肝脏的超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶活性随溶菌酶添加水平的增加总体呈现先升后降的变化趋势,L54组显著高于对照组(P0.05);各添加组丙二醛含量均显著低于对照组(P0.05)。研究表明,54和72mg/kg溶菌酶添加水平对氨氮应激下吉富罗非鱼的血清生化指标、抗菌活性和肝脏抗氧化指标产生了最积极有效的调控,能够增强鱼体的抗应激能力。     

Effects of antimicrobial peptides on growth,feed utilization,serum biochemical indices and disease resistance of juvenile shrimp,Litopenaeus vannamei

This experiment was conducted to assess the effects of antimicrobial peptides at different levels (Diet 1 (0%), Diet 2 (0.1%), Diet 3 (0.2%), Diet 4 (0.4%), Diet 5 (0.6%) and Diet 6 (1%)), on growth, serum biochemical indices and antioxidant effect, feed utilization and disease resistance in Litopeneaus vannamei. There were four replicates in each group in the experiment (mean weight = 0.21 ± 0.00 g) and also fed with their respective diets for 8 weeks. Growth performance compared with the control group (0%) significantly increased at first and then decreased among treatment groups (p < .05) whereas the survival rate ranged from 78% to 96%. No significant differences were observed in terms of moisture, crude protein and ash content, but there was a significant increase in crude lipid (p < .05). In serum, acid phosphatase, alkaline phosphatase, polyphenol oxidase, triglyceride, glucose and total cholesterol changed as compared with 0%. Total antioxidant capacity, glutathione peroxidase, catalase, superoxide dismutase and malondialdehyde were also different from 0%. Disease resistance was increased in shrimp among treatment groups with 0.4% recording the lowest mortality percentage of 37% after the challenge test. The results from the present study suggested that supplementation of AMP at 0.4% in shrimp diet can improve growth performance, antioxidants activities and innate immune response of Pacific whiteleg shrimp.     

Nitrate removal efficiency and bacterial community of polycaprolactone-packed bioreactors treating water from a recirculating aquaculture system

A denitrification reactor packed with polycaprolactone (PCL) as a carbon source and biofilm carrier was developed to remove nitrate nitrogen (\( {\mathrm{NO}}_3^{-}-\mathrm{N} \)) from the water of a recirculating aquaculture system for 115 days. The hydraulic retention time was set to 6 h, and the water flow rate was 0.95 L h^?1. The removal rates of \( {\mathrm{NO}}_3^{-}-\mathrm{N} \) ranged from 34.67 to 155.7 g \( {\mathrm{NO}}_3^{-}-\mathrm{N} \) m^?3 day^?1 when the effluent \( {\mathrm{NO}}_3^{-}-\mathrm{N} \) concentrations ranged between 1.62 and 72.25 mg L^?1. No obvious changes in the Fourier transform infrared spectra of the PCL before and after use were observed. Bacterial community structure in the biofilm of the PCL granules was analyzed using Illumina MiSeq sequencing technology. Proteobacteria and Bacteroidetes were predominant in the biofilm, with relative abundances of 63.9 and 27.4%, respectively. Three genera, Acidovorax, Azospira, and Diaphorobacter, were capable of both denitrification and PCL degradation. This study indicates that PCL-packed reactors may be used and optimized for removing nitrate from aquaculture effluents.     

枸杞叶(芽)茶降血糖作用小鼠实验研究

探讨枸杞叶茶、枸杞芽茶水煎剂对糖尿病小鼠的降血糖作用.采用的方法为制备四氧嘧啶糖尿病小鼠模型后,将其分为6组,即枸杞叶茶低剂量组、枸杞叶茶高剂量组,枸杞芽茶低剂量组、枸杞芽茶高剂量组,盐酸二甲双胍组及对照组.灌胃21d后,测定各组小鼠空腹血糖并进行比较.结果表明,枸杞叶茶低剂量组、枸杞芽茶低剂量组和盐酸二甲双胍组的降血糖作用相当,与对照组相比有显著差异(P<0.05).通过实验可以得出:一定剂量的宁夏枸杞叶茶、枸杞芽茶水煎剂对四氧嘧啶糖尿病小鼠具有明显的降血糖作用.     

几种松树挥发物对松材线虫行为的影响

通过室内生测的方法,研究了几种主要的松树挥发物对松材线虫行为的影响.结果表明,α-异松油烯、β-水芹烯、β-蒎烯抑制松材线虫繁殖,其中α-异松油烯、β-水芹烯抑制力较强,β-蒎烯抑制力较弱,而β-月桂烯、反式-石竹烯、莰烯、异长叶烯和α-蒎烯对线虫繁殖没有影响.β-水芹烯和莰烯具有杀松材线虫活性,β-蒎烯和β-月桂烯增强松材线虫活力,而异长叶烯、α-蒎烯、反式-石竹烯对线虫存活没有影响.β-蒎烯、反式-石竹烯、异长叶烯、莰烯对松材线虫具引诱作用,其中β-蒎烯引诱力最强,反式-石竹烯次之,异长叶烯、莰烯引诱力最弱,而α-蒎烯、β-水芹烯、α-异松油烯和β-月桂烯对线虫无引诱作用.并对几种挥发物在松树抗松材线虫病中的作用进行了讨论.     

优质蛋白玉米发展回顾与前景展望

优质蛋白玉米(QPM)是过去植物育种领域中取得最意想不到的成就之一,它的赖氨酸和色氨酸含量比普通玉米高,从而改善玉米蛋白质品质,因此其营养价值优于普通玉米。文章回顾了优质蛋白玉米的发展历史以及中国优质蛋白玉米研究的发展历程,提出政府支持,重视科技投入,加强研究力度,实施多学科合作尤其是现代生物技术的引进利用,加强种质资源创新与利用研究,把普通玉米自交系和杂种优势群转育为优质蛋白玉米资源,挖掘新的种质源,探索新的检测方法和应用领域,形成科研——种植——加工业——养殖业产业链联合开发是中国优质蛋白玉米研究的发展方向。     

基于ASM1的颗粒污泥对苯酚响应的数学模拟

以简化的ASM1为基础建立好氧颗粒污泥数学模型,通过苯酚的抑制作用进行模型修正,模拟苯酚对好氧颗粒污泥中微生物生长的影响。结果表明:苯酚浓度的增大有利于颗粒污泥中苯酚降解菌量的积累,但不利于其快速增长;由于苯酚的抑制作用自养菌和异养菌在颗粒外层的浓度低于在颗粒内层的浓度,而苯酚降解菌由于好氧生长和自抑制作用主要位于距颗粒中心1.2~1.8 mm处。