Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

The endometrium of sheep consists of plenty of raised aglandular areas called caruncular (C), and intensely glandular intercaruncular areas (IC). In order to better understand the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins (DEPs) (P < 0.05), functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on. Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.     

苜蓿褐斑病的离体叶接种研究

研究和鉴定苜蓿褐斑病抗病性的离体叶接种技术。结果表明,离体叶接种鉴定比传统的整株鉴定效率高,结果可靠。在0.3%水琼脂培养基中加入50μm/mL苯骈咪唑,即可达到最佳保绿效果。接种所需的适宜条件是,相对湿度接近100%,温度20℃,接种时间20h,无光照。培养所需的适宜条件是,生长箱内光照(光强度9000lux)和黑暗每12h交替一次,有光时22℃,无光时18℃。     

Mdivi-1, mitochondrial fission inhibitor,impairs developmental competence and mitochondrial function of embryos and cells in pigs

Mitochondria are highly dynamic organelles that undergo constant fusion/fission as well as activities orchestrated by large dynamin-related GTPases. These dynamic mitochondrial processes influence mitochondrial morphology, size and function. Therefore, this study was conducted to evaluate the effects of mitochondrial fission inhibitor, mdivi-1, on developmental competence and mitochondrial function of porcine embryos and primary cells. Presumptive porcine embryos were cultured in PZM-3 medium supplemented with mdivi-1 (0, 10 and 50 μM) for 6 days. Porcine fibroblast cells were cultured in growth medium with mdivi-1 (0 and 50 μM) for 2 days. Our results showed that the rate of blastocyst production and cell growth in the mdivi-1 (50 μM) treated group was lower than that of the control group (P < 0.05). Moreover, loss of mitochondrial membrane potential in the mdivi-1 (50 μM) treated group was increased relative to the control group (P < 0.05). Subsequent evaluation revealed that the intracellular levels of reactive oxygen species (ROS) and the apoptotic index were increased by mdivi-1 (50 μM) treatment (P < 0.05). Finally, the expression of mitochondrial fission-related protein (Drp 1) was lower in the embryos and cells in the mdivi-1-treated group than the control group. Taken together, these results indicate that mdivi-1 treatment may inhibit developmental competence and mitochondrial function in porcine embryos and primary cells.     

牛流行热病的诊疗

本文通过某公牛站4次流行热病的发病情况、临床症状、预防治疗及笔者的体会,对牛流行热病的预防和治疗提供参考。     

三种中草药复方提取物的体外抑菌试验

采用平皿稀释法研究三种中草药复方提取物对大肠杆菌及沙门氏菌的体外抑菌作用.试验结果表明,方Ⅰ、方Ⅱ、方Ⅲ对大肠杆菌的最小抑菌浓度(MIC)分别为0.02、0.02和0.01 g/mL,说明方Ⅲ对大肠杆菌的体外抑菌作用优于方Ⅰ、方Ⅱ;三种中草药方对沙门氏菌的最小抑菌浓度都为0.01 g/mL,说明三种方对沙门氏菌的抑菌效果相近;方Ⅰ、方Ⅱ对沙门氏菌的抑菌效果优于大肠杆菌,方Ⅲ对这两种菌抑菌效果相近;对大肠杆菌来说,中草药单方有效成分经定向提取后组方其抑菌效果比复方总提物好.     

Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages

Glutathione (GSH) concentrations of oocytes are considered as an important marker of the cytoplasmic maturation. The present study was designed to compare GSH concentrations of in vivo and in vitro matured canine oocytes. In vivo matured oocytes were collected 72 hr after ovulation by flushing fallopian tubes after laparotomy. Ovaries were collected from bitches with different reproductive stages, and collected oocytes were divided into 2 groups according to the size viz. < 120 microm and > 120 microm in diameter and cultured for 72 hr in Tissue Culture Medium-199 supplemented with 10% FBS, 2.2 mg/ml sodium bicarbonate, 2.0 microg/ml estrogen, 0.5 microg/ml FSH, 0.03 IU/ml hCG, and 1% penicillin-streptomycin solution in the presence or absence of 50 microM beta-mercaptoethanol. GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. GSH concentrations of immature canine oocytes were 2.9 and 3.8, 3.5 and 6.8, and 3.1 and 6.5 pM/oocyte for < 120 microm and > 120 microm in diameter oocyte groups at anestrous, follicular and luteal stage, respectively (P<0.05). In vivo matured oocytes had significantly higher GSH concentrations compared with in vitro matured oocytes. The GSH content was 19.2 pM/oocyte for in vivo matured oocytes, while 4.1 to 8.1 and 5.7 to 13.2 pM/oocyte for in vitro matured oocytes cultured in the absence or presence of beta-mercaptoethanol, respectively (P<0.05). Presence of beta-mercaptoethanol increased GSH synthesis in canine oocytes cultured in vitro, and oocytes collected from follicular and luteal stage was superior to anestrus oocytes.     

早开堇菜组织培养及植株再生体系的建立

以野生早开堇菜为材料,研究了不同外植体类型(子叶节、叶片、叶柄)和不同激素配比对其不定芽诱导、愈伤组织诱导和分化的影响,成功建立了早开堇菜组织培养植株再生体系。结果表明,1) 诱导子叶节和叶柄不定芽诱导的最适培养基为 MS+2.0 mg/L 6-BA+0.1 mg/L NAA,叶片不适合作为直接诱导不定芽的外植体。2) 3种外植体均能诱导愈伤组织,子叶节愈伤组织诱导的时间最短,其次为叶柄,叶片最晚。子叶节和叶柄愈伤组织诱导的最适培养基为MS+1.0 mg/L 6-BA+0.5 mg/L 2,4-D,诱导率分别为98.3%和96.7%;叶片愈伤组织诱导的最适培养基为MS+1.0 mg/L 6-BA+0.5 mg/L 2,4-D+0.05 mg/L NAA,诱导率可达88.3%。3) 最适愈伤组织分化培养基为:MS+1.5 mg/L 6-BA+0.1 mg/L NAA,分化率为100%。4)不定芽增殖的最佳培养基为:MS+1.5 mg/L 6-BA+0.075 mg/L NAA,增殖率为4.68。 5)再生苗移植到添加1.0 mg/L 6-BA的1/2 MS培养基上,生根率92.3%。6)组培苗移栽到珍珠岩∶河沙∶腐殖质(1∶2∶2)的混合基质时,100%成活,且植株长势好。     

四个绵羊品种MHC-DQA1基因exonⅡ多态性分析

为了比较和揭示洼地绵羊、杜泊羊、小尾寒羊及特克赛尔绵羊4个绵羊品种MHC-DQA1基因的遗传多态性,采用PCR-RFLP技术对4个绵羊品种224个样本的MHC-DQA1基因exonⅡ的315 bp片段进行多态性分析;并对基因型频率和等位基因频率进行统计,运用Popgen32软件计算相应的遗传参数.结果表明,AluI-R...     

乳源性益生元(百泰~-A)对西伯利亚鲟(acipenser baerii brandt)生长性能和营养成分消化率的影响

实验研究了不同剂量乳源性益生元(百泰-A)对西伯利亚鲟(Acipenser baerii Brandt)生长性能和营养成分消化率的影响。实验用西伯利亚鲟初始体重为(75±0.1)g,288尾鱼随机分4组,每组4个重复,每个重复18尾,实验周期为8周。基础饲料粗蛋白的含量为36%,粗脂肪为11%,总能为18.50 MJ/kg。实验料在此基础上分别添加0%(G0)、0.4%(G0.4)、0.8%(G0.8)和2%(G2)的百泰-A,各组饲料分别添加0.1%的Y2O3作为指示剂测定饲料营养成分表观消化率。实验结果显示:在饲料中添加百泰-A后各组西伯利亚鲟的增重率没有显著差异(P>0.05),饲料系数随乳源性益生元(百泰-A)的剂量增加而呈降低趋势,且G2组饲料系数显著低于对照组(G0)(P<0.05)。各组饲料干物质(88.2%)和蛋白质(64.8%)的消化率均没有显著影响(P>0.05);G2组总氨基酸(90.5%)的消化率显著高于G0.4组(88.8%)(P<0.05),与其他各组相比没有显著差异(P>0.05);G2组组氨酸(91.4%)、亮氨酸(90.8%)、苏氨酸(85.5%)、缬氨酸(89.5%)和赖氨酸(93.5%)的消化率显著高于对照组(P<0.05)。在西伯利亚鲟饲料中添加2%乳源性益生元(百泰-A)能够提高其对饲料中氨基酸的消化率和饲料利用率。