Exposed riverine sediments (ERS) in England and Wales: distribution,controls and management

相似文献   

Rescaling of aerial survey data with information from small numbers of telemetry tags to estimate the size of a declining harbour seal population

相似文献   

The action of herbicides on fatty acid biosynthesis and elongation in barley and cucumber

BACKGROUND: Herbicides that affect lipid metabolism have been used commercially for many years. Here, napropamide, diphenamid, dimethachlor and cafenstrole are compared; these have all been classified by the Herbicide Resistance Action Committee (HRAC) as K₃ herbicides and inhibitors of cell division and/or synthesis of very‐long‐chain fatty acids (VLCFAs). In addition, spiro‐decanedione A and pinoxaden dione are compared as inhibitors of lipid synthesis through inhibition of acetyl‐CoA carboxylase (ACCase). RESULTS: Whereas the chloracetamide dimethachlor and the carboxyamide cafenstrole potently inhibited VLCFA synthesis in both barley and cucumber, the acetamides napropamide and diphenamid which are also classified as K₃ herbicides and likewise the unclassified herbicide cinmethylin did not. The graminicide pinoxaden dione inhibited de novo fatty acid synthesis in barley, but not in cucumber, and correspondingly inhibited the plastid form of maize ACCase much more than the cytosolic form (IC₅₀ values of 0.1 and 17 µM ). By contrast, spiro‐decanedione A exhibited herbicidal effects not only on grasses but also on broad leaves, strongly inhibited maize cytosolic ACCase and inhibited synthesis of VLCFAs in cucumber. CONCLUSIONS: The acetamides napropamide and diphenamid, which do not inhibit VLCFA synthesis, should be classified separately from K₃ herbicides that do. Pinoxaden dione and spiro‐decanedione A represent new classes of chemicals acting on plant lipid synthesis. Copyright © 2010 Society of Chemical Industry     

Oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis

Canine parvoviral enteritis (CPE) is a severe disease characterized by systemic inflammation and immunosuppression. The function of circulating phagocytes (neutrophils and monocytes) in affected dogs has not been fully investigated. We characterized the functional capacity of canine phagocytes in CPE by determining their oxidative burst and phagocytic activities using flow cytometry. Blood was collected from 28 dogs with CPE and 11 healthy, age-matched, control dogs. Oxidative burst activity was assessed by stimulating phagocytes with opsonized Escherichia coli or phorbol 12-myristate 13-acetate (PMA) and measuring the percentage of phagocytes producing reactive oxygen species and the magnitude of this production. Phagocytosis was measured by incubating phagocytes with opsonized E. coli and measuring the percentage of phagocytes containing E. coli and the number of bacteria per cell. Complete blood counts and serum C-reactive protein (CRP) concentrations were also determined. Serum CRP concentration was negatively and positively correlated with segmented and band neutrophil concentrations, respectively. Overall, no differences in phagocyte function were found between dogs with CPE and healthy control dogs. However, infected dogs with neutropenia or circulating band neutrophils had decreased PMA-stimulated oxidative burst activity compared to healthy controls. Additionally, CPE dogs with neutropenia or circulating band neutrophils had decreased PMA- and E. coli–stimulated oxidative burst activity and decreased phagocytosis of E. coli compared to CPE dogs without neutropenia or band neutrophils. We conclude that phagocytes have decreased oxidative burst and phagocytic activity in neutropenic CPE dogs and in CPE dogs with circulating band neutrophils.     

加快仔猪的早期生长

通过饲喂的方法促使断奶仔猪早期快速增重，可改善直到屠宰为止的生长模式而不危害胴体价值。     

High throughput,high resolution selection of polymorphic microsatellite loci for multiplex analysis

Background  

Large-scale genetic profiling, mapping and genetic association studies require access to a series of well-characterised and polymorphic microsatellite markers with distinct and broad allele ranges. Selection of complementary microsatellite markers with non-overlapping allele ranges has historically proved to be a bottleneck in the development of multiplex microsatellite assays. The characterisation process for each microsatellite locus can be laborious and costly given the need for numerous, locus-specific fluorescent primers.