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排序方式: 共有219条查询结果,搜索用时 15 毫秒
71.
Mutsumi NAKAMURA Takafumi NAGAMINE Akio TAKENAKA Rustem I. AMINOV Koretsugu OGATA Kiyoshi TAJIMA Hiroki MATSUI Yoshimi BENNO Hisao ITABASHI 《Animal Science Journal》2002,73(5):347-352
A 2.6-kb DNA fragment encoding a xylanase gene ( xyn A) was cloned from the rumen hemicellulolytic bacterium Ruminococcus albus 7. The deduced primary structure of the protein (XynA) was divided into a signal peptide region and 3 domains. Domain A was identified as a family 11 (G) catalytic domain, but one amino acid residue was replaced by another in an active site signature 1 of family 11. Domain B is a stabilizing domain for the catalytic domains of families 10 and 11. Deletion of domain B reduced stability of the xylanase at high temperature and at high and low pH. Domain B may be useful for protein engineering of xylanase. Domain C has sequence similarity to deacetylases and NodB proteins. 相似文献
72.
Makoto OTSUKA Tomoko MUTO Yu KATSUNUMA Yutaka NAKAMURA Ryozo TAKADA Hajime MINATO 《Animal Science Journal》2002,73(6):497-503
The effects of the administration of four Lactobacillus strains as probiotics on the constitution of microbial populations in the intestine of pigs were investigated by using a litter of pigs. The experimental group was fed a diet blended with the freeze‐dried powder containing lactobacilli at a concentration of 0.2% (W/W). After 4 weeks of probiotics administration, the total viable count of anaerobes in the feces was not significantly different between the pigs fed a diet supplemented with probiotics and the pigs given the non‐supplemented diet. However, viable counts of eubacteria were significantly higher (P < 0.01) in the feces of pigs fed a diet supplemented with probiotics than in the pigs given the non‐supplemented diet. Conversely, viable counts of clostridia were significantly lower (P < 0.01) in the feces of pigs fed a diet supplemented with probiotics than the pigs given the non‐supplemented diet. The total volatile fatty acid concentrations in the feces of pigs that were given a diet supplemented with probiotics were significantly lower (P < 0.05) than those given the non‐supplemented diet. Further, the molar proportion of n‐butyric acids in the feces of pigs that were given a diet supplemented with probiotics, was significantly lower (P < 0.05) than those given the non‐supplemented diet. 相似文献
73.
The effects of medetomidine and xylazine on gastrointestinal motility and gastrin release in the dog
K. NAKAMURA S. HARA & N. TOMIZAWA 《Journal of veterinary pharmacology and therapeutics》1997,20(4):290-295
The effect of medetomidine, a potent and highly selective α2 -adrenoceptor agonist, on the motility of the gastric antrum, duodenum, mid-jejunum and ileum was investigated in ten dogs. Its effect on the release of gastrin was also determined. Administration of medetomidine intramuscularly (i.m.) at a dose of 40 μg/kg inhibited the motility of the gastric antrum, duodenum, mid-jejunum and ileum significantly, in comparison to administration of xylazine intramuscularly at a dose of 2.0 mg/kg. The release of gastrin was also significantly decreased in dogs receiving medetomidine. It was found to inhibit the motility in the gastric antrum and duodenum longer than in the mid-jejunum and ileum, presumably by acting specifically on α2 -adrenoceptors, likely at the peripheral level. Medetomidine also inhibited the gastric contraction associated with gastrin secretion. 相似文献
74.
The effect of parental vitamin K deficiency on developing bone structure was examined in mummichog ( Fundulus heteeroclitus ) larvae. Larvae from fish fed a vitamin K-deficient diet had abnormal vertebral formation, whereas larvae from fish fed a vitamin K-rich diet showed a significantly lower incidence of abnormality 5 d post-hatch. Larvae from fish that had been fed either the vitamin K-rich or the vitamin K-deficient diet had a high rate of abnormality when reared on a vitamin K-deficient diet for 30 d. Larvae from fish fed a vitamin K-rich diet that were reared on a vitamin K-rich diet showed a low rate of abnormality. Vertebral bone structure of larvae fed a vitamin K-deficient diet for 30 d was thin and rough, and seemed to have repaired fine fractures of the bone surface. In contrast, this type of structural abnormality was not observed in any larvae fed a vitamin K (phylloquinone) rich diet. These results imply that vitamin K deficiency causes the formation of thin and weak bone, and induces bone structure abnormality both in early development and during growth. 相似文献
75.
Glucose is utilized for oxidation and synthesis of various lipids in cultured rat embryos. The present experiment examined the effect of insulin on the incorporation of glucose into lipid fractions in rat embryos in vitro . Embryos at the 2-cell, 8-cell and blastocyst stages were incubated for 5 h in hamster embryo culture medium (HECM)-1 containing 14 C-glucose and 170 nmol/L insulin, or in HECM-1 containing only 14 C-glucose, and the oxidation of glucose in these embryos was examined. In addition, the total lipids of blastocysts were separated by thin layer chromatography and the radioactivity of the separated lipid fractions was measured. Oxidation of glucose was significantly increased after insulin treatment compared with that without insulin treatment in 8-cell embryos and blastocysts ( P < 0.05), but not in 2-cell embryos. Incorporation of glucose into lipids in blastocysts was significantly lowered by insulin treatment compared with that without insulin treatment ( P < 0.05). Most of the radioactivity was recovered from triacylglycerols of blastocysts and the remaining radioactivity was found in other neutral lipids and phospholipids. We conclude that insulin accelerates the utilization for oxidation of glucose and inhibits the storage of triacylglycerols in rat blastocysts. 相似文献
76.
Miho Shimizu Yasunori Nakajima Kazuo Matsuya Makoto Kimura 《Soil Science and Plant Nutrition》2013,59(4):595-600
Azolla has been used as a green manure for rice in Vietnam and southern China (3). Recently it was adopted by farmers in the Koronadal area in South Cotabato Province, the Philippines (2). In that area Azolla pinnata grows reasonably well without phosphate fertilizer, which is generally used for stimulating Azolla growth (3, 5). 相似文献
77.
Miho Shimizu Motoki Hayashi Kazuo Matsuya Jun Murase Makoto Kimura 《Soil Science and Plant Nutrition》2013,59(6):877-881
Abstract The amount and composition of phospholipid fatty acids (PLFAs) in the percolating water taken from different depths of soil (10 cm, PW10; 40 cm, PW40) and floodwater (FW) in a paddy field were compared during the period of rice cultivation. The amounts of PLFAs in PW10, PW40, and FW ranged from 22.6 to 46.2 μg L?1, from 22.3 to 54.5 μg L?1, and from 82.9 to 179.0 μg L?1, respectively. The PLFA profiles in PW10, PW40, and FW were similar to each other and 16 : 1ω7c, 18 : 1ω7, and 16 : 0 PLFAs were dominant components, irrespective of the sampling site and sampling time. High proportions of straight mono-unsaturated PLFAs ranging from 42.0 to 76.5% suggested that Gram-negative bacteria were the major members in the microbial communities of the water samples studied. A potential indicator of the environmental stresses imposed upon the microbiota that was represented by the trans vs. cis ratio of 16 : 17 PLFA was constantly low (< 0.05), indicating that the microbial communities at these sites were hardly stressed. 相似文献
78.
79.
Minami W. OKUYAMA Michito SHIMOZURU Go ABE Mariko NAKAI Mariko SASHIKA Ken-Ichiro SHIMADA Nobuhiro TAKAHASHI Daisuke FUKUI Ryohei NAKAMURA Toshio TSUBOTA 《The Journal of reproduction and development》2013,59(4):361-367
The raccoon (Procyon lotor), indigenous to North America, has
naturalized in Japan as an invasive alien species, having been introduced into the country
in the 1970s. In Hokkaido, the northernmost island of Japan, feral raccoons have been
increasing in number and spreading throughout the island. The age at the onset of puberty
for raccoons is important for estimating individual lifetime reproductive success and
population growth. The present study investigated the timing of and potential factors
affecting the onset of puberty in male raccoons in Hokkaido. External characteristics and
histology of testes were studied in 151 male feral raccoons and in 1 captive juvenile. For
the majority of feral yearling raccoons, prepubertal development began in May, and
spermatozoa production began in October prior to their second mating season. However, some
larger juveniles attained puberty during the juvenile period. The captive juvenile, which
was fed throughout the winter, attained puberty only 11 months after birth. These results
suggest that if male raccoons can achieve enough body growth before the first mating
season, puberty can be attained early. In both juveniles and yearlings, spermatozoa
production was only observed after autumn. This timing coincided with the recrudescence of
seasonally active spermatogenesis in adult males. Therefore, attaining puberty in male
raccoons appears to require both adequate body nutrient development and several
environmental factors that control seasonal testicular changes. 相似文献
80.
Overexpression of the LASAP2 gene for secretory acid phosphatase in white lupin improves the phosphorus uptake and growth of tobacco plants 总被引:1,自引:0,他引:1
Jun WASAKI Hayato MARUYAMA Miho TANAKA Takuya YAMAMURA Hiraki DATEKI Takuro SHINANO Susumu ITO Mitsuru OSAKI 《Soil Science and Plant Nutrition》2009,55(1):107-113
Secretion of acid phosphatase (APase) from the roots to take up phosphorus (P) is a well-known strategy of plants under P-deficient conditions. White lupin, which shows vigorous growth in low-P soils, is noted for its ability to secrete APase under P-deficient conditions. The APase secreted by white lupin roots is stable in soil solution and shows low substrate specificity, suggesting that genetic modification of plants using the APase gene LASAP2 might improve their ability to use organic P. The objective of the present study was to evaluate the potential of LASAP2 transgenic plants to increase organic P utilization. Dry matter production and P accumulation were higher in LASAP2 transgenic tobacco plants grown in gel media containing soluble phytate as the sole P source than in wild-type tobacco plants. Phosphorus uptake by the transgenic plants also increased in soil culture conditions. LASAP2 was apparently more effective in the liberation of organic P, including phytate, in the soil than the native tobacco APase. Thus, the enzymatic stability of LASAP2 in the soil appears to be an important factor for P acquisition. 相似文献