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排序方式: 共有219条查询结果,搜索用时 15 毫秒
211.
Yamato SAJIKI Satoru KONNAI Reiko NAGATA Satoko KAWAJI Hayato NAKAMURA Sotaro FUJISAWA Tomohiro OKAGAWA Naoya MAEKAWA Yukinari KATO Yasuhiko SUZUKI Shiro MURATA Yasuyuki MORI Kazuhiko OHASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):162
Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic enteritis of ruminants. Previous studies have shown that programmed death-ligand 1 (PD-L1) is associated with the disease progression, and PD-L1 blockade activates MAP-specific Th1 responses in vitro. Here, we performed anti-PD-L1 antibody administration using 2 MAP-infected cattle at the late subclinical stage of infection. After administration, bacterial shedding was reduced or maintained at a low level. Additionally, MAP-specific Th1 cytokine production was upregulated, and CD69 expression was increased in T cells. Collectively, the treatment has a potential as a novel control method against Johne’s disease. 相似文献
212.
ABSTRACT: To clarify faunal overlap between a seagrass bed and adjacent coral and sand areas, and the number of reef fishes utilizing the seagrass bed as juvenile habitat, visual censuses were conducted at Amitori Bay, Iriomote Island, Japan. The numbers of species and individuals of fishes were significantly higher in the coral area than in the seagrass bed and sand area. Cluster and ordination analyses based on the number of individuals of each species demonstrated that the fish assemblage structure differed among the three habitats in each season, but with some overlaps. Approximately half the seagrass bed fishes occurred in the adjacent coral area (coral–seagrass species). Dominant species of coral–seagrass species utilized the seagrass bed as an important juvenile habitat. Thus, some overlaps in habitat use were present between the seagrass bed and adjacent coral area. Despite such overlaps, however, coral–seagrass species accounted for only approximately 15% of coral reef fishes overall, indicating that most of the latter hardly utilize the seagrass bed directly in the study area. 相似文献
213.
Abstract. This study was initiated to determine the cause(s) of delayed mortality in newly captured skipjack tuna, Katsuwonus pelamis (L.), being held at the National Marine Fisheries Service Kewalo Research Facility. Sixty-four per cent of 244 skipjack tuna delivered to the facility died, usually on the second or third day after capture. The capture history, morphological data, serum chemistry (21 standard parameters), haematology, and histological samples of major organs, were obtained from 30 fish sampled at sea immediately after capture, or after approximately 4, 9, 24, 48 or 500+ h in captivity. The cause(s) of death in these fish could not be attributed to anoxia, disseminated intravascular coagulation, lactic acidosis, capture myopathy or infection. Post-capture haemodilution is hypothesized as a major factor of delayed capture mortality syndrome in skipjack tuna. 相似文献
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Yoshiaki NAKAMURA Mariko TASAI Kumiko TAKEDA Keijiro NIRASAWA Takahiro TAGAMI 《The Journal of reproduction and development》2013,59(6):580-587
The Japanese quail (Coturnix japonica) is a valuable bird as both
an experimental animal, for a wide range of scientific disciplines, and an
agricultural animal, for the production of eggs and meat. Cryopreservation of PGCs
would be a feasible strategy for the conservation of both male and female fertility
cells in Japanese quail. However, the effects of freeze-thaw treatment on viability,
migration ability and germline transmission ability of quail PGCs still remain
unclear. In the present study, male and female PGCs were isolated from the blood of
2-day-old embryos, which were cooled by slow freezing and then cryopreserved at –196
C for 77–185 days, respectively. The average recovery rate of PGCs after
freeze-thawing was 47.0%. The viability of PGCs in the frozen group was significantly
lower than that of the control group (P<0.05) (85.5% vs. 95.1%).
Both fresh and Frozen-thawed PGCs that were intravascularly transplanted into
recipient embryos migrated toward and were incorporated into recipient gonads,
although the number of PGCs settled in the gonads was 48.5% lower in the frozen group
than in the unfrozen control group (P<0.05). Genetic cross analysis revealed that
one female and two male recipients produced live progeny derived from the
frozen-thawed PGCs. The frequency of donor-derived offspring was slightly lower than
that of unfrozen controls, but the difference was not significant (4.0
vs. 14.0%). These results revealed that freeze-thaw treatment
causes a decrease in viability, migration ability and germline transmission ability
of PGCs in quail. 相似文献
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219.
Hirohito OGAWA Kenji OHYA Raphael AYIZANGA Hiroko MIYAMOTO Asako SHIGENO Masao YAMADA Yasuhiro TAKASHIMA Miho INOUE-MURAYAMA Ayato TAKADA Boniface BABOREKA KAYANG 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(11):1491
Some filoviruses such as ebolaviruses and marburgviruses, cause hemorrhagic fever in humans and nonhuman primates. Pigs are suggested to play a potential role in the filovirus ecology. We investigated the seroprevalence of filovirus infection in pigs in Ghana. Using a viral glycoprotein (GP)-based enzyme-linked immunosorbent assay, we detected filovirus-specific immunoglobulin G antibodies in 5 of 139 samples. These positive sera showed specificities to four different filovirus species. Particularly, two of the positive sera reacted to GPs of two African ebolaviruses (i.e., Ebola virus and Taï Forest virus) in Western blotting. Our results suggest that these Ghanaian pigs were exposed to multiple filoviruses and emphasize the importance of continuous monitoring of filovirus infection in pig populations in West African countries. 相似文献