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991.
The evolution of clay soil porosity is currently demonstrated via the shrinkage curves in a large water content domain spreading from a shrinkage limit to a liquidity limit. In fact, the parallel between in situ profiles and the shrinkage curves in such a large water content range is difficult to obtain because of the lack of earth pressure in the laboratory tests and in situ limited water contents. The vertical distribution of porosity throughout a clay-rich marsh soil profile was studied in a grassland field with samples taken from the soil surface characterized by water contents near their shrinkage limit down to 2.00 m deep saturated sediments over their liquidity limit. The depth of the plasticity limit isolates a soil in a solid state characterized by a vertical prism-like structure from a plastic to pseudo-liquid state in depth. The porosity was calculated from the measurements of the density of intact samples by double weighing and image analysis of 100 cm2 polished sections. The initial structure of clay soil was maintained by impregnation based on water–acetone–resin exchange. An ultraviolet photo luminescent pigment added to the resin allowed the capture of images from which shrinkage cracks and microporosity of the clay matrix were easily separated. The distribution of porosity between the shrinkage crack mesoporosity and the clay matrix microporosity was evaluated after the mathematical decomposition of the grey level curves characteristic of each level. Vertical evolution of the porosity distribution from the soil surface in a solid state to the plastic and pseudo-liquid sediment in depth was presented on the shrinkage curve of the clay material. The measurements point out how the clay matrix microporosity and mesoporosity of shrinkage cracks are complementary and the role of the scale effect on the shrinkage curve. The analysis of images captured on an optical microscope under polarized and analyzed light and the SEM observation of freeze-dried samples demonstrated the isotropic arrangement of the clay particles in typical “honey-comb” architecture in the in situ plastic-to-liquid saturated domain. Eventually the distribution of porosity through the profile results from the evolution of the initial “honey-comb” microstructure of the sediment induced by the desiccation phenomenon. It is governed by the depth of plasticity limit of the clay material and by the depth of the water table. 相似文献
992.
Garna H Mabon N Wathelet B Paquot M 《Journal of agricultural and food chemistry》2004,52(15):4652-4659
A new method for the determination of the main neutral sugars in pectin has been developed. The sample preparation involves a mild chemical attack followed by an enzymatic hydrolysis. The completeness and nondestructive character of the method are demonstrated by comparison of the results obtained with different acids such as H2SO4, HCl, and trifluoroacetic acid (TFA) at different concentrations (2, 1, or 0.2 M) at two temperatures (80 or 100 degrees C). The chemical hydrolysis of pectin neutral sugar chains with strong acid (1 or 2 M) and high temperature (100 degrees C) shows that the liberation of the pectin sugars is not realized at the same rate for each sugar. Different optimum conditions are thus obtained. However, the chemical pectin hydrolysis with 0.2 M TFA at 80 degrees C is characterized by the liberation of pectin neutral sugar side chains without any degradation within 72 h of hydrolysis. Under these conditions, the liberation of some pectin sugars, essentially galactose, glucose, and rhamnose, was not complete. An enzymatic hydrolysis is necessary to obtain a complete release of all the sugars. The combination of the two treatments, a chemical hydrolysis realized with diluted acid (0.2 M) for 72 h at low temperature (80 degrees C) on one hand and an enzymatic hydrolysis on the other hand, allow a total liberation of pectin sugars. The quantitative analysis of the carbohydrates is realized with accuracy, high selectivity, and sensitivity with high-performance anion-exchange chromatography with pulsed-amperometric detection. The sugars can be analyzed without any derivatization with a limit of quantification of 0.1 mM. 相似文献
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Observed phenotypic differences of lipid content between strains of rainbow trout and their hybrids cultured in small Canadian prairie lakes are related to differences in the size of fish and highly correlated with differences in growth rate. As a percent of dry matter mean lipid levels of different strains and their hybrids ranged from 32.5% to 45.9% in one experiment and from 11.6% to 24.3% in another. There were significant additive genetic differences in lipid content between strains, independent of differences in weight, and there also appeared to be significant non-additive × environment interactions.Phenotypic differences in percent dry matter were small compared to the differences in lipid content. 相似文献
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