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111.
112.
Michiko NAKAI Manabu OZAWA Naoki MAEDOMARI Junko NOGUCHI Hiroyuki KANEKO Junya ITO Akira ONISHI Naomi KASHIWAZAKI Kazuhiro KIKUCHI 《The Journal of reproduction and development》2014,60(3):256-259
In pigs, the embryonic developmental ability after intracytoplasmic sperm injection (ICSI) is inferior to that resulting from
in vitro fertilization (IVF). We evaluated the timing of cell division up to blastocyst formation on
embryonic development after ICSI using either whole sperm (w-ICSI) or the sperm head alone (h-ICSI) and IVF as a control. At
10 h after ICSI or IVF, we selected only zygotes, and each of the zygotes/embryos was evaluated for cleavage every 24 h until
168 h. We then observed a delay in the 1st and 2nd cleavages of h-ICSI embryos and also in blastocoele formation by w-ICSI
embryos in comparison with IVF embryos. The rate of blastocyst formation and the quality of blastocysts in both ICSI groups
were inferior to those in the IVF group. In conclusion, the delay in cleavage of porcine ICSI embryos shows poorer embryonic
development. 相似文献
113.
114.
Alagu?Manickavelu Kanako?Kawaura Hisako?Imamura Michiko?Mori Yasunari?OgiharaEmail author 《Euphytica》2011,177(2):179-190
Genetic maps are useful for analysis of quantitative trait loci (QTLs) and for marker-assisted selection (MAS) in breeding.
A simple sequence repeat (SSR) marker linkage map of common wheat was constructed based on recombination inbred lines (RILs)
derived from a cross between Chinese Spring and spelt wheat. The map included 264 loci on all wheat chromosomes covering 2,345.2 cM
with 962, 794.6, and 588.6 cM for the A, B, and D genomes, respectively. Using the RILs and the map, we detected 42 putative
QTLs on 15 chromosomes for ear length, spikelet number, spike compactness, kernel length, kernel width, kernel height and
β-glucan content. Each QTL explained 4–45% of the phenotypic variation. Five QTL cluster regions were detected on chromosomes
1A, 5AL, 2B, 2D, and 4D. The first QTLs for β-glucan content in wheat were identified on chromosomes 3A, 1B, 5B, and 6D. 相似文献