全文获取类型
收费全文 | 82篇 |
免费 | 14篇 |
专业分类
综合类 | 9篇 |
水产渔业 | 2篇 |
畜牧兽医 | 71篇 |
植物保护 | 14篇 |
出版年
2023年 | 1篇 |
2020年 | 1篇 |
2019年 | 5篇 |
2018年 | 6篇 |
2017年 | 5篇 |
2016年 | 4篇 |
2015年 | 4篇 |
2014年 | 3篇 |
2013年 | 17篇 |
2010年 | 3篇 |
2009年 | 4篇 |
2008年 | 3篇 |
2007年 | 4篇 |
2006年 | 3篇 |
2005年 | 1篇 |
2004年 | 2篇 |
2003年 | 2篇 |
2001年 | 3篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 4篇 |
1997年 | 6篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1992年 | 4篇 |
1990年 | 3篇 |
1989年 | 2篇 |
排序方式: 共有96条查询结果,搜索用时 658 毫秒
91.
Effects of Serum Deprivation and Cycloheximide on Cell Cycle of Low and High Passage Porcine Fetal Fibroblasts 总被引:1,自引:0,他引:1
MD Goissis HVA Caetano MG Marques FRO de Barros WB Feitosa MP Milazzotto M Binelli MEOA Assumpção JA Visintin 《Reproduction in domestic animals》2007,42(6):660-663
Arrest of cells in G0/G1 cell cycle phase is desired for nuclear transfer procedures. Serum starvation and cell cycle inhibitors are different ways to induce synchronization of the cell cycle. This study investigated the effects of serum starvation and cycloheximide (CHX) on the cell cycle of low (5th) and high (15th) passages fetal porcine fibroblasts. Cell cycle phases were determined using fluorescent activated cell sorting. Fifth passage fibroblast cultures had higher (p < 0.05) proportion of cells in G0/G1 only after 72 h of serum starvation (77.60 ± 0.65) when compared with non‐starved cells (71.44 ± 1.88). Serum starvation for all periods tested induced an increase (p < 0.05) on proportion of cells in G0/G1 on the 15th passage. No significant differences were observed on the 5th passage cultures exposed to CHX, although, on the 15th passage an increase on proportion of cells was observed after all periods of exposure (p < 0.05). These data indicates that high passage cells in vitro are more susceptible to serum starvation and CHX G0/G1 synchronization. 相似文献
92.
Histopathology of experimental plague in cats 总被引:4,自引:0,他引:4
Formalin-fixed paraffin-embedded archival tissues of seven adult cats of both sexes that died after being experimentally infected with Yersinia pestis were examined light microscopically to characterize the lesions. The cats were exposed in two groups using two routes of infection: ingestion of Y. pestis-infected rodent or a subcutaneous injection of Y. pestis to simulate a flea bite. Immunohistochemistry was performed on tissues from all organ systems from a representative cat from each group to determine the distribution of Y. pestis bacilli during infection. In all seven cats, bubonic plague lesions were seen. The lesions of pneumonic plague were present in two cats. Septicemic plague was confirmed in all seven cats by bacteriologic culture. Aggregations of bacteria were seen in lymphoid tissue in all cats and in lung tissues from the two cats with pneumonic plague. The most consistent histologic finding was necrosuppurative inflammation in the lymph nodes. Invariably, Y. pestis bacteria were present in large numbers at affected sites. Orally infected cats had more numerous lesions in the lymph nodes of the head and neck regions. These experimentally induced cases of feline plague document that cats are unique among carnivores in exhibiting bubonic, pneumonic, and septicemic plague following exposure to Y. pestis. The lesions of the orally infected cats were consistent with those previously described for naturally occurring Y. pestis infections in cats and corroborate the contention that cats most commonly contract plague by eating Y. pestis-infected rodents and not via flea bite. The histopathology of Y. pestis disease in these cats is comparable to that described for human plague. 相似文献
93.
Embryonic and Early Foetal Losses in Cattle and Other Ruminants 总被引:1,自引:0,他引:1
94.
95.
Iacome SC Jácome Francisca GC Sousa Candice MG De Leon Denis A Spricigo Mauro MS Saraiva Patricia EN Givisiez Wondwossen A Gebreyes Rafael FC Vieira Celso JB Oliveira 《Veterinary research》2014,45(1)
This investigation reported for the first time the occurrence of intramammary infections caused by Staphylococcus in primiparous replacement goats before parturition and the persistence of clinical Staphylococcus aureus infection during the lactation period. Subclinical infections, mainly caused by coagulase negative staphylococci (CoNS), did not persist during lactation. Genotyping analysis indicated that environment seems to play a moderate role as source of intramammary infections to goats before parturition, but causative agents of mastitis in lactating animals are not genotypically related to environmental staphylococci. The occurrence and persistence of intramammary infections in replacement goats demonstrate the need to consider those animals as potential sources of infections in dairy goat herds. 相似文献
96.
Termiticidal lectins from Myracrodruon urundeuva (Anacardiaceae) cause midgut damage when ingested by Nasutitermes corniger (Isoptera: Termitidae) workers
下载免费PDF全文
![点击此处可从《Pest management science》网站下载免费的PDF全文](/ch/ext_images/free.gif)