干扰对毕棚沟生态旅游区土壤物理性质的影响

通过对四川毕棚沟生态旅游区的游径、景区公路沿线和火烧迹地等3种干扰类型的土壤物理性质进行典型抽样调查及分析,样地土壤剖面特征表现为： 3类干扰均导致样地土壤含水率呈现不同程度的下降,且表层土壤含水量远高于中下层;表层土壤较为疏松,板结程度小于中下层,踩踏更易导致表层土壤的板结;表层土壤孔隙度大于中下层,踩踏会严重降低土壤表层孔隙度,使土壤逐渐板结;土壤中下层石砾含量明显高于上层。     

Effects of HIF-1α/SDF-1 signaling axis on hypoxia-induced migration and adhesion of progenitor cells

AIM: To observe the expression of hypoxia-inducible factor-1alpha (HIF-1α) and stromal cell-derived factor 1 (SDF-1) in pulmonary artery endothelial cells (PAECs) of SD rats and to investigate the role of HIF-1α/SDF-1 signaling axis on hypoxia-induced migration and adhesion of progenitor cells to PAECs. METHODS: Immunomagnetic beads were used to separate and purify the CD34⁺/CXCR4⁺ progenitor cells derived from the peripheral circulation of SD rats. The expression of HIF-1α and SDF-1 in PAECs exposed to hypoxia (1% O₂, 5% CO₂ and 94% N₂) was detected by immunofluorescence, Western blotting and ELISA. The migration index and adhesion rate were measured in the progenitor cells, which were subjected to the following different treatments: (1) normoxia (21% O₂); (2) hypoxia 12 h; (3) hypoxia 12 h +HIF-1α inhibitor (2ME2); (4) hypoxia 12 h+SDF-1 neutralizing antibody; (5) hypoxia 12 h+2ME2+SDF-1 neutralizing antibody.RESULTS: The expression of HIF-1α and SDF-1 in PAECs was effectively induced by the hypoxic exposure, and both of them reached the peak levels after 12 h of hypoxic treatment (P<0.01), while administration of 2ME2 decreased the hypoxia-induced SDF-1 expression (P<0.05). Treatment of the PAECs with 2ME2 or SDF-1 neutralizing antibody attenuated the migration index and adhesion rate of progenitor cells to the PAECs (P<0.05). CONCLUSION: There is a HIF-1α/SDF-1 signaling axis in hypoxia-exposed PAECs, which may play a crucial role in the migration and adhesion of progenitor cells to PAECs.     

高温胁迫下不同耐热性萝卜幼苗生理响应分析

利用耐热性差异显著的萝卜自交系为材料,研究了高温(38℃)胁迫下萝卜幼苗丙二醛(MDA)、游离脯氨酸、可溶性蛋白和可溶性糖含量变化与耐热性的关系。结果表明:高温处理前耐热材料中可溶性蛋白含量高于不耐热材料;可溶性糖、游离脯氨酸和MDA含量无显著差异。处理24、48、72h,耐热材料中可溶性蛋白、可溶性糖和游离脯氨酸含量均显著高于不耐热材料,MDA含量显著低于不耐热材料。处理24h,4个指标的含量都比处理前显著增加,可溶性蛋白含量在处理48、72h时逐渐降低,但耐热材料下降得较慢。与处理24h相比,处理48、72h时可溶性糖、游离脯氨酸和MDA含量在耐热材料中没有显著变化,而在不耐热材料中处理48h显著高于处理24h时的含量。表明这4个指标的含量变化能准确反映萝卜幼苗耐热性差异,可考虑用于萝卜幼苗的耐热性鉴定评价。     

单增李斯特菌双元调控系统lmo1060/lmo1061缺失株的构建及其抗酸应激能力

为了探明双元调控系统Lmo1060/Lmo1061是否影响单增李斯特菌(LM)的抗应激作用及致病性,本研究以LM 10403S为亲本株,通过同源重组技术构建了 Δlmo1060/lmo1061缺失株.应激存活试验结果表明,lmo1060/lmo1061缺失显著增强LM在酸应激(pH=2.5)条件下的存活率,但不影响该菌...     

Somatic embryogenesis and Agrobacterium-mediated transformation of Japanese apricot (Prunus mume) using immature cotyledons

This study describes a successful method of somatic embryogenesis and genetic transformation using immature cotyledons of Prunus mume. Immature cotyledons from four different developmental stages of eight different P. mume cultivars were used for the experiments to optimize somatic embryogenesis and genetic transformation protocols. Somatic embryogenesis was induced when the explants were cultured on somatic embryo inducing medium consisting of MS basic medium supplemented with 1 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 μM 6-benzyladenine (BA). They were cultured for 30 days and then transferred to somatic embryo propagation medium containing 0.1 μM α-naphthaleneacetic acid (NAA) and 5 μM BA. It appeared that the developmental stage of the immature cotyledons used as explants was the most important factor for somatic embryogenesis; higher frequencies of somatic embryogenesis were observed when the immature cotyledons were less than 5 mm in length regardless of cultivars. For genetic transformation, the immature cotyledons were inoculated with Agrobacterium tumefaciens EHA101 harbouring a binary plasmid vector with neomycin phosphotransferase II and an intron-interrupted β-glucuronidase gene under the control of cauliflower mosaic virus 35S promoter, and three transgenic plant lines were obtained from inoculated “Sirakaga” immature cotyledons. Transgenic somatic embryos and shoots were selected using 25 mg l⁻¹ kanamycin. Integration of transgenes in the genome of GUS-positive putative transgenic shoots was confirmed by PCR and Southern blot analyses.     

Expression of β-catenin in patients with chronic myeloid leukemia

AIM: To observe the expression of β-catenin in patients with chronic myeloid leukemia (CML) at different disease phases, and to analyze the relationship between BCR-ABL and cytogenetic response to imatinib mesylate. METHODS: RT-PCR and Western blotting were used to detect β-catenin mRNA and protein expression in bone marrow mononuclear cells (BMMNCs) from 99 patients with CML. The association with BCR-ABL and BCR-ABL fusion was determined by FISH in 94 patients after one year treatment with imatinib mesylate, and the relationship between β-catenin and cytogenetic response to imatinib mesylate was analyzed. RESULTS: The expression of β-catenin was increased significantly in patients with blast crisis and accelerated phase (P<0.01), while the expression of β-catenin between normal person and chronic phase of CML patients was not statistically different (P>0.05). No significant relation between β-catenin and BCR-ABL expression (r=0.314, P>0.05) was observed. The expression of β-catenin was increased significantly in the patients who did not reach main cytogenetic remission (P<0.01). CONCLUSION: The patients in progression phases of CML over-express β-catenin. The expression of β-catenin is not significantly related to BCR-ABL expression, but related to the therapeutic response of imatinib. Beta-catenin may be involved in the mechanism of CML progression and could be used as a new therapeutic target.     

不同品种苜蓿芽期对重金属镉的耐性研究

以5个苜蓿品种为材料,研究镉胁迫对苜蓿种子发芽及幼苗生长的影响,并分析比较了5个品种间的耐镉性差异。结果表明,低浓度镉对种子的发芽率和发芽指数影响不大,甚至有促进作用。随着镉浓度的增加,5个苜蓿品种的种子发芽率、发芽指数和活力指数、胚根和芽长都呈明显下降趋势,而胚芽直径则随着镉浓度的增加先呈上升趋势,而后又在50mg/L的镉浓度下迅速下降。通过耐镉性系数的计算,综合各个指标比较得出5个品种苜蓿的芽期耐镉性,排名为:新疆大叶苜蓿牧歌401号苜蓿0104号苜蓿玛纳斯苜蓿佩里苜蓿。     

番茄黄化曲叶病毒的鉴定与群体进化分析

番茄黄化曲叶病(tomato yellow leaf curl disease,TYLCD)是番茄上的重要病害,可由多种双生病毒引起。为了明确北京地区番茄黄化曲叶病的病毒种类和病毒基因组变异情况,本研究收集了51个采自北京不同区县的病毒疑似样品,进行了双生病毒的检测及分离物的基因组分析。经双生病毒简并引物检测25个样品呈阳性;扩增获得10个代表性分离物的全基因组,大小均为2 781bp,共含有6个开放阅读框;经比对,10个分离物与Tomato yellow leaf curl virus-Israel(TYLCV-IL)同源率大于99%;基于我国TYLCV分离物全基因组序列的变异分析表明,TYLCV在进化上比较保守,整个基因组上的变异是不均匀的;系统发育树显示我国TYLCV分离物在进化树上可分为3个组群,分析了我国TYLCV群体进化情况。     

牛初乳与牛常乳中乳脂肪球膜蛋白质的组成及功能的对比研究

利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate polyacrylamide gel electropheresis,SDS-PAGE)将牛初乳与牛常乳中乳脂肪球膜蛋白质的组成部分进行分离鉴定,发现牛初乳与牛常乳中乳脂肪球膜蛋白质的组成存在较大的差异,且在牛初乳中鉴定出628种乳脂肪球膜蛋白质,牛常乳中鉴定出487种乳脂肪球膜蛋白质.由基因本体(gene ontology,GO)功能注释分析发现,在生物过程中生物调控作用是牛初乳和牛常乳中乳脂肪球膜的主要生物过程.在分子功能上,牛初乳的乳脂肪球膜蛋白质的绑定作用大于牛常乳.在细胞组成上,牛初乳乳脂肪球膜蛋白质参与细胞外区域远远大于牛常乳.通过京都基因与基因组百科全书系统(Kyoto Encyclopedia of Genes and Genomes,KEGG)代谢通路分析可知,牛初乳和牛常乳乳脂肪球膜蛋白质参与的代谢途径不同,表明牛初乳在日后的生产加工中更具有利用价值.