全文获取类型
收费全文 | 704篇 |
免费 | 81篇 |
国内免费 | 1篇 |
专业分类
林业 | 22篇 |
农学 | 12篇 |
基础科学 | 3篇 |
70篇 | |
综合类 | 119篇 |
农作物 | 18篇 |
水产渔业 | 49篇 |
畜牧兽医 | 425篇 |
园艺 | 14篇 |
植物保护 | 54篇 |
出版年
2023年 | 16篇 |
2022年 | 13篇 |
2021年 | 34篇 |
2020年 | 52篇 |
2019年 | 38篇 |
2018年 | 23篇 |
2017年 | 32篇 |
2016年 | 25篇 |
2015年 | 13篇 |
2014年 | 20篇 |
2013年 | 21篇 |
2012年 | 29篇 |
2011年 | 54篇 |
2010年 | 18篇 |
2009年 | 19篇 |
2008年 | 30篇 |
2007年 | 35篇 |
2006年 | 25篇 |
2005年 | 41篇 |
2004年 | 34篇 |
2003年 | 11篇 |
2002年 | 8篇 |
2001年 | 9篇 |
2000年 | 13篇 |
1999年 | 6篇 |
1998年 | 4篇 |
1996年 | 4篇 |
1993年 | 4篇 |
1989年 | 6篇 |
1988年 | 7篇 |
1987年 | 8篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1984年 | 10篇 |
1983年 | 7篇 |
1982年 | 4篇 |
1979年 | 6篇 |
1978年 | 5篇 |
1977年 | 8篇 |
1976年 | 6篇 |
1975年 | 5篇 |
1974年 | 4篇 |
1973年 | 8篇 |
1972年 | 5篇 |
1971年 | 7篇 |
1969年 | 3篇 |
1968年 | 5篇 |
1967年 | 3篇 |
1966年 | 6篇 |
1943年 | 3篇 |
排序方式: 共有786条查询结果,搜索用时 15 毫秒
111.
112.
Western blot detection of PrP Sc in archived paraffin-embedded brainstem from scrapie-affected sheep
Robert A Kunkle Eric M Nicholson Semakaleng Lebepe-Mazur Dennis L Orcutt Megan L Srinivas Justin J Greenlee David P Alt Amir N Hamir 《Journal of veterinary diagnostic investigation》2008,20(4):522-526
Scrapie is a naturally occurring fatal neurodegenerative disease of adult sheep and goats, one of a group of mammalian diseases known as transmissible spongiform encephalopathies (TSE) or prion diseases. Immunoassays that identify disease-associated prion protein (PrP Sc) are integral to the diagnosis of scrapie and other prion diseases. Results obtained by either immunohistochemistry (IHC) or Western blot (WB) assay are generally adequate for the definitive diagnosis. Approved or accepted methods for WB diagnosis of TSEs requires the use of fresh or frozen nonfixed tissue samples, whereas formalin-fixed, paraffin-embedded tissue is required for the localization of PrP Sc by IHC. Because disparate processing methods are used for these accepted diagnostic techniques, separate tissue samples are collected from the same animal. Occasions arise in which there is either insufficient quantity of tissue available to complete analysis by both techniques or initial tissue processing is incompatible with one of the assays. Also, results between the assays may differ because of the vagaries of sampling, especially in case material that contains moderate-to-low levels of PrP Sc. The present article describes a method to conduct a WB assay from the same paraffin-embedded brainstem sample used for the IHC diagnosis of experimentally induced sheep scrapie. 相似文献
113.
Megan J. McLane DVM John W. Schlipf Jr. DVM MS Marco L. Margiocco DMV MS Howard Gelberg DVM PhD 《Journal of Veterinary Cardiology》2008,10(2):141-145
An alpaca presented for emergency evaluation of respiratory distress. Physical examination revealed weakness, pyrexia, and a grade V/VI left apical holosystolic murmur. Initial thoracic examination and radiographs were consistent with left sided congestive heart failure. Blood cultures, echocardiographic evaluation, and necropsy findings established a diagnosis of bacterial endocarditis associated with a Listeria monocytogenes infection. Left ventricular endocarditis is uncommon in ruminants, and to the authors' knowledge there are no other reports of L. monocytogenes being the causative agent in an alpaca. 相似文献
114.
Jeremy D Edwards Jaroslav Janda Megan T Sweeney Ambika B Gaikwad Bin Liu Hei Leung David W Galbraith 《Plant methods》2008,4(1):13
Background
We report the development of a microarray platform for rapid and cost-effective genetic mapping, and its evaluation using rice as a model. In contrast to methods employing whole-genome tiling microarrays for genotyping, our method is based on low-cost spotted microarray production, focusing only on known polymorphic features. 相似文献115.
Megan J. Dornbusch Ryan F. Limb Caley K. Gasch 《Strength and Conditioning Journal》2018,71(6):691-694
Invasive species control requires understanding the mechanisms behind their establishment and their interactions with other species. One potential ecosystem alteration influencing the establishment and spread of invasive species is anthropogenic nitrogen enrichment, from sources like introduced or invasive nitrogen (N)-fixing legumes, which can alter competition between native, non-native, and invasive plants. Kentucky bluegrass (Poa pratensis) and N-fixing yellow sweet clover (Melilotus officinalis) are exotic to the Great Plains and are currently invading and degrading native rangelands by altering ecosystem processes and displacing native plants. Therefore, we investigated how N enrichment from yellow sweet clover affects the aboveground biomass production of Kentucky bluegrass and western wheatgrass (Pascopyrum smithii), a native cool-season grass, the ranges of which overlap in the northern Great Plains. In a controlled greenhouse environment, we conditioned experimental pots by growing yellow sweet clover and terminating each plant after 8 wk. Conditioned soils contained ≈ 340% more plant-available N than untreated soils 2 wk after yellow sweet clover death. We then grew Kentucky bluegrass and western wheatgrass transplant seedlings in interspecific and intraspecific pairs in pots conditioned either with or without yellow sweet clover for 12 wk. Aboveground biomass production of both Kentucky bluegrass and western wheatgrass grown in interspecific and intraspecific pairs increased in conditioned soils. However, when grown together in conditioned pots, the increase in Kentucky bluegrass biomass relative to untreated pots (520%) was double that of the increase in western wheatgrass biomass (260%). Our results reveal that Kentucky bluegrass can use increased soil N to produce proportionally more aboveground biomass than western wheatgrass, a native grass competitor. Thus, our results suggest yellow sweet clover and other sources of N enrichment may facilitate the invasion of Kentucky bluegrass. 相似文献
116.
117.
118.
Temperature modulates liver lipid accumulation in Atlantic salmon (Salmo salar L.) fed low dietary levels of long‐chain n‐3 fatty acids
下载免费PDF全文
![点击此处可从《Aquaculture Nutrition》网站下载免费的PDF全文](/ch/ext_images/free.gif)
N.H. Sissener B.E. Torstensen M.A.G. Owen N.S. Liland I. Stubhaug G. Rosenlund 《Aquaculture Nutrition》2017,23(4):865-878
Atlantic salmon (Salmo salar) were fed five graded levels of eicosapentaenoic acid (EPA, 20:5n‐3) and docosahexaenoic acid (DHA, 22:6n‐3), from 1.4 to 5.2% of total fatty acids (FA, 5–17 mg kg?1 feed), and grew from ~160 g to ~3000 g, with the period from 1450 g onwards conducted both at 6 °C and at 12 °C. All fish appeared healthy, and there were no diet‐related differences in haematological or plasma parameters, as well as intestinal histological or gut microbiota analysis. Fish reared at 6 °C had higher accumulation of storage lipids in the liver compared to fish reared at 12 °C. Liver lipids also increased with decreasing dietary EPA + DHA at 6 °C, while there was no such relationship at 12 °C. Gene expression of SREBP1 and 2, LXR, FAS and CPT1 could not explain the differences in liver lipid accumulation. In liver polar lipids, DHA was found to be reduced when dietary EPA + DHA was <2.7% of FAs, while the level of EPA in the membranes was not affected. In conclusion, reducing dietary EPA + DHA from 5.2 to 1.4% of total FAs had a minor impact on fish health. Temperature was the factor that most affected the liver lipid accumulation, but there was also an interaction with dietary components. 相似文献
119.
Reproductive resilience: a paradigm shift in understanding spawner‐recruit systems in exploited marine fish
下载免费PDF全文
![点击此处可从《Fish and Fisheries》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Susan Lowerre‐Barbieri Greg DeCelles Pierre Pepin Ignacio A Catalán Barbara Muhling Brad Erisman Steven X Cadrin Josep Alós Andres Ospina‐Alvarez Megan M Stachura Michael D Tringali Sarah Walters Burnsed Claire B Paris 《Fish and Fisheries》2017,18(2):285-312
A close relationship between adult abundance and stock productivity may not exist for many marine fish stocks, resulting in concern that the management goal of maximum sustainable yield is either inefficient or risky. Although reproductive success is tightly coupled with adult abundance and fecundity in many terrestrial animals, in exploited marine fish where and when fish spawn and consequent dispersal dynamics may have a greater impact. Here, we propose an eco‐evolutionary perspective, reproductive resilience, to understand connectivity and productivity in marine fish. Reproductive resilience is the capacity of a population to maintain the reproductive success needed to result in long‐term population stability despite disturbances. A stock's reproductive resilience is driven by the underlying traits in its spawner‐recruit system, selected for over evolutionary timescales, and the ecological context within which it is operating. Spawner‐recruit systems are species specific, have both density‐dependent and fitness feedback loops and are made up of fixed, behavioural and ecologically variable traits. They operate over multiple temporal, spatial and biological scales, with trait diversity affecting reproductive resilience at both the population and individual (i.e. portfolio) scales. Models of spawner‐recruit systems fall within three categories: (i) two‐dimensional models (i.e. spawner and recruit); (ii) process‐based biophysical dispersal models which integrate physical and environmental processes into understanding recruitment; and (iii) complex spatially explicit integrated life cycle models. We review these models and their underlying assumptions about reproductive success vs. our emerging mechanistic understanding. We conclude with practical guidelines for integrating reproductive resilience into assessments of population connectivity and stock productivity. 相似文献
120.
James W. Wynne Chris Stratford Joel Slinger Francisca Samsing Megan Rigby Russell McCulloch Petra Quezada-Rodriguez Richard S. Taylor 《Journal of fish diseases》2020,43(1):39-48
Hydrogen peroxide (H2O2) is a commonly used treatment for a range of parasitic diseases of marine finfish, including amoebic gill disease (AGD). While this treatment is partially effective at reducing parasite load, H2O2 can have detrimental effects on the host under certain conditions. Treatment temperature and dose concentration are two factors that are known to influence the toxicity of H2O2; however, their impact on the outcome of AGD treatment remains unclear. Here, we investigated the effects of treatment temperature (8, 12 or 16°C) and dose concentration (750, 1,000, 1,250 mg/L) on the efficacy of H2O2 to treat AGD. We demonstrated that a 20-min bath treatment of H2O2 at all doses reduced both parasite load and gross gill score significantly. Parasite load and gross gill score were lowest in the 1,000 mg/L treatment performed at 12°C. At the high dose and temperature combinations, H2O2 caused moderate gill damage and a significant increase in the plasma concentration of electrolytes (sodium, chloride and potassium). Taken together, our study demonstrates that higher H2O2 treatment temperatures can adversely affect the host and do not improve the effectiveness of the treatment. 相似文献