Age-related functional changes in the follicle-associated epithelium of the bursa of Fabricius in Shaver Cockerels

A study of the age-related functions of immunologically important components of the bursa of Fabricius in Shaver cockerels showed that endocytosis of carbon particles by the specialised follicle-associated epithelium was at a high level from hatching until 5 weeks of age and thereafter declined until at 18 weeks it could no longer be detected. The follicle-associated epithelium had marked non-specific esterase activity during the first 15 weeks of life as determined by a standard acid alpha-naphthyl acetate esterase method. The absolute weight of the bursa was at a maximum at 9 to 10 weeks. Involution began before 14 weeks and was complete by 22 weeks. The results indicate that the critical period for the bursa in regard to acquiring immunity from either local vaccination or environmental challenge is likely to be within the first five weeks of life.     

小麦脱植基相关基因在春性小麦叶片叶绿素降解过程中的作用分析

小麦叶片的衰老会导致产量的损失,而叶绿素降解是小麦叶片衰老的明显特征,分析小麦叶绿素降解过程中脱植基反应的相关基因叶绿素酶（TaCLH）和脱镁叶绿素水解酶（TaPPH）在春性小麦叶片衰老过程中的作用,为解析小麦叶绿素降解的分子机制提供参考。以10个春性小麦品种为参试材料,对衰老过程中TaCLH和TaPPH的相对表达量进行测定,结合不同品种在开花后不同时期的相对叶绿素含量（SPAD）、功能绿叶面积（GLAD）和叶绿素荧光参数的变化规律,研究叶片衰老过程中TaCLH和TaPPH与SPAD、GLAD和叶绿素荧光参数的相关关系。结果表明,TaPPH的相对表达量与GLAD、SPAD及叶绿素荧光参数[ETR、F_v/F_m、Y(Ⅱ)]等生理指标之间存在极显著负相关关系,与TaCLH相对表达量存在极显著正相关关系,表明TaPPH在春小麦叶绿素降解过程的脱植基反应中起主要作用。     

No-tillage and direct seeding for super hybrid rice production in rice–oilseed rape cropping system

No-tillage and direct seeding (NTDS) is an effective crop production method for reducing production costs and soil conservation. In order to understand performance of super hybrid rice under NTDS in rice–oilseed rape cropping system, a researcher-managed trail (2004–2010) and an on-farm research (2002–2005) were conducted to compare different tillage (conventional tillage or no-tillage) and rice establishment methods (transplanting or direct seeding) in super hybrid rice–oilseed rape cropping system. Under researcher-managed condition, grain yields of super hybrid rice under NTDS and conventional tillage and transplanting (CTTP) were equal. Compared with under CTTP, super hybrid rice under NTDS was characterized by more panicle number per m² but less spikelet number per panicle, and lower aboveground biomass production before heading but higher aboveground biomass accumulated during heading to maturity. Higher maximum tiller number per m² and lower spikelet production efficiency were responsible for the more panicle number per m² and less spikelet number per panicle under NTDS, respectively. Under farmer-managed condition, super hybrid rice under NTDS had more panicle number m² than under CTTP, which resulted in higher grain yield. Labor input under NTDS was lower than that under CTTP. Moreover, adoption of NTDS for super hybrid rice production had no significant impacts on seed yield and yield components of oilseed rape in rice–oilseed rape cropping system. Our study showed that CTTP could be replaced with NTDS to maintain yield and save labor for super hybrid rice production in rice–oilseed rape cropping system.     

Sustainable urbanization in Southeast Asia and beyond: Challenges of population growth,land use change,and environmental health

As economic growth centres, the Southeast Asian cities feature high population pressure, unsustainable land use, environmental degradation, and large ecological footprints. It is difficult to manage environmental health and basic services for urban dwellers, and ensure optimum flow of ecosystem services in the context of rapid, unplanned, and haphazard urbanization. These challenges are particularly multifaceted in the developing countries of Southeast Asian region. This study, based on secondary sources, adopted multidisciplinary lenses, such as geographical information systems, socio‐economic perspective, and sustainability science to examine the population situation, land use change pattern, and drivers of environmental degradation in the Southeast Asian cities as well as the Dhaka megacity, and brought forth a fresh perspective to look into contemporary urban ecosystems, population dynamics, environmental health, and sustainability. It also focused on identifying the commonalities among the cities under study to create a common understanding towards promoting collaborative urban development. This study shows that the urbanization process in the Southeast Asia region is taking place mostly in an unplanned and haphazard manner. With little concern for nature, life‐supporting ecological systems, and the environment, urban spatial growth continues unabated. The data surveyed and discussed in this paper shows that the current style of urbanization in Southeast Asia can best be called unsustainable. The findings also suggest that the general wellbeing and welfare of the current and future generations in Southeast Asian cities as well as in Dhaka is at risk. The paper recommends concerted efforts towards making the urbanization process sustainable, including better urban planning, policymaking, and international and regional cooperation.     

Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants

Kenaf (Hibiscus cannabinus L.; Family: Malvaceae), is multipurpose crop, one of the potential alternatives of natural fiber for biocomposite materials. Longer fiber and higher cellulose contents are required for good quality biocomposite materials. However, average length of kenaf fiber (2.6 mm in bast and 1.28 mm in whole plant) is below the critical length (4 mm) for biocomposite production. Present study describes whether fiber length and cellulose content of kenaf plants could be enhanced by increasing GA biosynthesis in plants by overexpressing Arabidopsis thaliana Gibberellic Acid 20 oxidase (AtGA20ox) gene. AtGA20ox gene with intron was overexpressed in kenaf plants under the control of double CaMV 35S promoter, followed by in planta transformation into V36 and G4 varieties of kenaf. The lines with higher levels of bioactive GA (0.3–1.52 ng g⁻¹ fresh weight) were further characterized for their morphological and biochemical traits including vegetative and reproductive growth, fiber dimension and chemical composition. Positive impact of increased gibberellins on biochemical composition, fiber dimension and their derivative values were demonstrated in some lines of transgenic kenaf including increased cellulose content (91%), fiber length and quality but it still requires further study to confirm the critical level of this particular bioactive GA in transgenic plants.     

Farmers' perceptions of and responses to environmental change in southwest coastal Bangladesh

Coastal Bangladesh is highly vulnerable to climate change and salinisation; hence, farm‐level adaptation is critically important. Farmers' perceptions of and responses to environmental change were investigated in two villages in Khulna District. Perceived environmental trends included increases in temperature, extreme weather events, soil toxicity, erratic rainfall and scarcity of water for irrigation. Perceptions of climate trends were consistent with measured trends in Khulna. On‐farm adaptation strategies included adjusting planting dates, excavating trenches in rice‐fields, adopting new crops, salinity‐reducing technologies, livestock‐rearing and home‐yard cropping. Non‐farm adaptation strategies included wage employment, short‐term migration and self‐employment. Adaptation was facilitated by income‐earning opportunities, training, and credit, and impeded by lack of access to water, markets, capital, and extension services. Farmers suggested policy support for dissemination of stress‐tolerant cultivars, access to irrigation, and price stabilisation or crop insurance to assist adaptation. While the study shows an impressive degree of awareness and adaptation, external support is needed to increase adaptive capacity.     

Quantitative trait loci analysis for net ginning energy requirements in upland cotton (<Emphasis Type="Italic">Gossypium hirsutum</Emphasis> L.)

Cotton cultivars with reduced fiber-seed attachment force have the potential to be ginned faster with less energy. The objective of this study was to identify quantitative trait loci (QTL) for net ginning energy requirement (NGE), and its relationship with other fiber quality traits in upland cotton. Two cotton lines, TAM 182-34 ELS and AR 9317-26, with significant differences in NGE and fiber-seed attachment force, were crossed and 285 F₂ plants derived from a single F₁ plant were planted in the field and leaf samples collected for DNA marker analysis (Population A). Individual F₃ plants and the two parents were planted in replicated progeny rows. The cotton was ginned on a 10-saw laboratory gin stand. Electrical power used by the gin was measured and recorded with a Yokogawa CW121 power meter. Fiber quality attributes were measured using a high volume instrument. A total of 455 SSR marker loci were used to construct a linkage map. Two QTLs were identified for NGE on chromosomes 12 and 20, associated with markers CIR148 and DPL0600, explaining 14 and 8.8% of the phenotypic variation, respectively. NGE shared the same genomic region with fuzz percent on chromosome 12. Population B, consisting of 260 F₂ progeny from the reciprocal cross AR 9317-26 X TAM 182-34 ELS, was used to confirm these QTLs by analyzing SSR markers mapped on Chrs 12 and 20. These QTLs (qNGE-c12 and qNGE-c20) were confirmed and appeared stable. Further validation of significantly associated markers on different populations is necessary prior to implementation in marker-assisted selection.     

Interactive effects of dietary astaxanthin and cholesterol on the growth,pigmentation, fatty acid analysis,immune response and stress resistance of kuruma shrimp (Marsupenaeus japonicus)

A 56‐day feeding trial was carried out to evaluate the interactive effects of chemically synthesized astaxanthin (Ax) and cholesterol (CHO) on the performances of kuruma shrimp juveniles. Six experimental diets containing 2 levels of Ax (0 and 0.6 g/kg diet) and 3 levels of CHO (0, 6 and 20 g/kg diet) were formulated in 2 × 3 factorial design. The results indicated that the interactions between dietary CHO and Ax were not significant on survival, growth performances, body proximate analysis, immune response and freshwater stress test. Significantly, better pigmentation performances and Ax contents of different parts of juveniles (whole body, muscle, carapace and head) were observed in higher Ax (Ax_0.6)‐supplemented groups. Meanwhile, interactions of dietary Ax and CHO were also found on colour reading parameters and whole‐body Ax contents. In addition, juveniles fed with the diet containing Ax at 0.6 g/kg diet in addition to CHO at 0, 6 and 20 g/kg diet showed better performances on digestive enzyme activities (protease, lipase and amylase) of hepatopancreas than 0 g Ax/kg diet supplemented with CHO at 0, 6 and 20 g/kg diet. It was concluded that dietary Ax and CHO functioned interactively.     

Dietary supplementation of garlic (Allium sativum) modulates gut microbiota and health status of tilapia (Oreochromis niloticus) against Streptococcus iniae infection

This study was conducted to characterize the causative agent of streptococcosis in tilapia (Oreochromis niloticus) and control of Streptococcus infection by means of garlic (Allium sativum) supplementation. The morphological, biochemical and polymerase chain reaction amplification confirmed 11 isolates belong to Streptococcus iniae from the infected fish eyes and tissue samples. Random screening of 12 well‐known medicinal plant parts against S. iniae revealed the garlic extract as the most effective herbal recovery against Streptococcus infection. In vivo challenge test with dietary supplementation of garlic powder significantly improved survival rates of fish against S. iniae infections, and modulate the microbial community and cytokine gene expression profiling in the intestine of the experimental tilapia. Among the two garlic supplemented treatments, 1.0 g garlic supplemented diet significantly increased (p < 0.05) the survival rates of tilapia and the gut bacterial operational transitional units abundance for Proteobacteria and Tenericutes, the phyla associated with healthy intestinal flora. The bacterial diversity index also found high with garlic supplemented diets. Significant upregulations of IL‐10 and IL‐17F gene expression in the intestinal tissue were observed with 1.0 g garlic supplemented diet where IL‐8 and IL‐1β expression levels were relatively static. The dietary supplementation of garlic, therefore, could be effective in the prevention of S. iniae infection in fish.