Effects of infusion time and addition of milk on content and absorption of polyphenols from black tea

Epidemiological studies assessing the health benefits of drinking black tea are equivocal. Such disparity may reflect an inability of semiquantitative assessment to consider how infusion time and addition of milk affect the bioavailability of potentially beneficial antioxidant polyphenols. Six brands of tea demonstrated similar increases in antioxidant capacity and total phenolic and catechin contents with increasing infusion time. These results were unaffected by the addition of milk. Consumption of black tea (400 mL) was associated with significant increases in plasma antioxidant capacity (10%) and concentrations of total phenols (20%), catechins (32%), and the flavonols quercetin (39%) and kaempferol (45%) (all p < 0.01) within 80 min. This was unaffected by adding milk. Infusion time may therefore be a more important determinant in the absorption of polyphenols from black tea. Observational studies assessing the health benefits of tea consumption require recording of brewing methods as well as frequency of consumption.     

Preliminary evidence for biologic activity of toceranib phosphate (Palladia(®)) in solid tumours

The purpose of this study was to provide an initial assessment of the potential biologic activity of toceranib phosphate (Palladia?, Pfizer Animal Health, Madison, NJ, USA) in select solid tumours in dogs. Cases in which toceranib was used to treat dogs with apocrine gland anal sac adenocarcinoma (AGASACA), metastatic osteosarcoma (OSA), thyroid carcinoma, head and neck carcinoma and nasal carcinoma were included. Clinical benefit (CB) was observed in 63/85 (74%) dogs including 28/32 AGASACA [8 partial response (PR), 20 stable disease (SD)], 11/23 OSAs (1 PR and 10 SD), 12/15 thyroid carcinomas (4 PR and 8 SD), 7/8 head and neck carcinomas [1 complete response (CR), 5 PR and 1 SD] and 5/7 (1 CR and 4 SD) nasal carcinomas. For dogs experiencing CB, the median dose of toceranib was 2.8 mg kg(-1) , 36/63 (58.7%) were dosed on a Monday/Wednesday/Friday basis and 47/63 (74.6%) were treated 4 months or longer. Although these data provide preliminary evidence that toceranib exhibits CB in dogs with certain solid tumours, future prospective studies are necessary to define its true activity.     

Will future climate change threaten a range restricted endemic species, the quokka (Setonix brachyurus), in south west Australia?

Range-restricted species, such as regional endemics, possess traits that may make them particularly vulnerable to environmental change. The quokka, Setonix brachyurus, is a small macropod, endemic to south-western Australia and two adjacent islands. Climatic factors appear to play a role in defining the distribution of this species. Mainland populations are historically restricted to areas with an annual average rainfall in excess of 700 mm and their current distribution is almost completely confined within the 1000 mm rainfall isohyet. As such, the predicted increasing aridity of south-western Australia due to climate change is likely to threaten the continued persistence of the quokka on the mainland. To examine this possibility, we modelled the distribution of the quokka with Maxent using records of occurrence and a combination of historical climate (1961-1990) and habitat variables. Future projections of this distribution were then examined assuming two simple dispersal scenarios (zero and full migration) and three climate-change scenarios of increasing severity for 2030, 2050 and 2070. The predictive performance of the distribution model generated under historical climate conditions was high (AUC > 0.8), with annual precipitation contributing the most information to the model. Except for the low-severity climate-change scenario under the full dispersal assumption, the future projected distribution of quokka was shown to contract over time. The extent of range contraction tended to increase with the severity of the climate-change scenario, with the species predicted to lose almost all range by the year 2070 under the most extreme climate-change scenario. The results indicate the importance of identifying potential refuges for the quokka (i.e. areas where the species is predicted to persist) and defining management strategies to protect these areas from threatening processes.     

3D-visualization and analysis of macro- and meso-porosity of the upper horizons of a sodic, texture-contrast soil

The lower E and upper B horizons of sodic, texture-contrast soils are a formidable barrier to most annual and many perennial crops. The research presented here is part of a wider study into the nature of subsoil constraints to root exploration. The aim of this study was to characterize in three dimensions the macro- and meso-porosity across the E horizon–Btn horizon interface of a sodic, texture-contrast soil using X-ray computed tomography (CT). Intact soil cores of 50-cm length and 15-cm diameter were scanned with a medical CT X-ray machine. The pore volume reconstructed from these scans had a resolution of 0.3 × 0.3 × 0.4 cm (in the x, y, z dimensions, respectively). This resolution allowed visualization and quantification of the macroporosity of the intact cores. Undisturbed samples of 1.5-cm diameter and 4-cm length were carefully excised from the interface and scanned with micro-CT X-ray equipment. The reconstructed pore volumes had an isotropic resolution of 19 μm that allowed analysis of the mesoporosity just on the boundary between the E and Btn horizons. Mesoporosity decreased across the interface and increased lower in the Btn horizon. The distribution of the pores at the macro- and meso-scales showed the importance of the smaller pores in the A and E horizons, whereas most of the porosity in the Btn horizon was attributed to the larger pores. Pores in this sodic, texture-contrast soil were not distributed homogeneously at either the macro- or meso-scale. A greater proportion of the pores in the E–B interface were horizontal than in the upper A1, upper E and lower Btn horizons. Some 'coiling' of the pores was also apparent in the interface. The shape of some pores (long tubular pores) suggested formation by roots as they drilled through the soil. The orientation of these pores was a function of physical (and possibly chemical) impedance at the interface.     

The challenge and status of IWRM in four river basins in Europe and Asia

This paper assesses the implementation of four selected IWRM principles in four very different river basins in Europe and Asia. The four principles relate to all the different aspects of sustainable development—environmental, social, economic and institutional—as well as the factor that is particularly crucial in many countries of the South: implementation capacity. The paper is based on the work performed in the EC-funded STRIVER project, “Strategy and methodology for improved IWRM—An integrated interdisciplinary assessment in four twinning river basins”. The four basins—Tungabhadra and Sesan (in Asia), and Tagus and Glomma (in Europe) exemplify very different problems and challenges with regard to IWRM: different levels of socio-economic development and very varying problems with regard to water quality and availability. The paper shows that the implementation of IWRM is at a fairly early stage in all the four STRIVER basins; and that successful implementation of water resources is dependent not only on the existence of relevant policies, but also the degree to which laws and policies are in fact implemented.     

Passive immunization of cyclic mares against androgen: gonadotropin and progesterone concentrations and estrous characteristics

Antiserum generated in a horse against testosterone conjugated to bovine serum albumin (BSA) was administered to six lighthorse mares (androgen-immunized mares) 1 to 3 d before a prostaglandin-induced estrus and twice again at 2-d intervals. Six control mares were administered antiserum generated against BSA on the same schedule. Relative to testosterone, cross-reactivities of other steroids with the testosterone antiserum were (%): dihydrotestosterone, 52; 5 alpha-androstane-3 alpha,17 beta-diol, 8.6; androst-4-ene-3,17-dione, 1.2; and all others tested less than .1. Tritiated testosterone binding in plasma increased (P less than .01) in androgen-immunized mares within 1 h and remained elevated (P less than .01) relative to controls for greater than 21 d. There was no effect (P greater than .10) of passive immunization against androgen on interval to estrus after prostaglandin injection, duration of estrus, ovarian volume, number of palpable follicles or follicular volume during estrus. In contrast, concentrations of luteinizing hormone (LH) were higher (P less than .05) in androgen-immunized mares than in control mares during estrus and early diestrus. Concentrations of follicle stimulating hormone (FSH) and progesterone at those times were not affected (P greater than .10). From these data, we conclude that androgens in the mare during estrus may be involved with the regulation of LH secretion. In contrast, no involvement with FSH secretion was apparent under these short-term conditions.