The effect of medetomidine and its antagonism with atipamezole on stress-related hormones, metabolites, physiologic responses, sedation, and analgesia in goats

Six 3‐year‐old goats (three males and three females) weighing 60.0 ± 18 kg (mean ± SD) were used to investigate the effect of medetomidine (MED; 20 µg kg^?1 IV) and its antagonism with atipamezole (ATI; 100 µg kg^?1 IV) on physiologic responses (heart rate (HR; beats minute^?1), respiratory rate (RR; breaths minute^?1), electrocardiogram (ECG), rectal temperature (T; °C), blood pressure (oscillometric; mm Hg), sedation (SED), posture (REC), analgesia (ALG), and stress‐related hormonal and metabolic responses (epinephrine and norepinephrine (high performance liquid chromatography with electrochemical detection), cortisol (COR; µg dL^?1; radioimmunoassay), glucose (GLU; mg mL^?1; enzymatic colorimetric assay), and free fatty acids (modified enzymatic colorimetric assay)); each goat received ATI or SAL in random order separated by 1 week. Jugular catheters were placed for drug administration and blood sampling (10–12 mL sample^?1) using a lidocaine skin block (20 mg) 2 hours prior to beginning of each trial; during this trial, goats breathed room air. Physiologic parameters were measured, SED, REC, and ALG were scored, and blood samples were collected from jugular catheters at baseline (time = ?30 minutes), 5 minutes post‐MED administration (time = ?25 minutes), 25 minute post‐MED administration and immediately prior to antagonism (time = 0 minute), and at 5, 30, 60, and 120 minutes after administering ATI or SAL. ALG was tested by clamping the withers and metacarpus with hoof testers fitted with a force transducer to measure applied isometric force (lb) (a technique used previously in goats to evaluate analgesia). Continuous variables were analyzed by Repeated Measures analysis of variance (anova ); categorical data were analyzed using a Friedman Repeated Measures anova on ranks. A p‐value of <0.05 was considered significant. If a significant difference was found, a Dunnett's pair‐wise comparison of means was conducted. Differences between ATI and SAL were examined at 5, 30, 60, and 120 minutes using a paired t‐test with a Bonferroni correction. Administration of MED resulted in a decrease in T (38.7 ± 0.3 to 34.5 ± 0.4 °C), HR (78 ± 19 to 55 ± 9), and RR (31 ± 12 to 14 ± 5) over time; an increase in mean arterial blood pressure (90 ± 19 to 132 ± 23), COR (0.254 ± 0.125 to 4.327 ± 1.233), and GLU (82.0 ± 13.2 to 255.9 ± 38.9); and changes in SED (alert to marked sedation), REC (standing to recumbent), and ALG (metacarpus = 5 ± 2 to 14 ± 0; withers = 3 ± 2 to 14 ± 0). GLU was 62–70% higher at 60 and 120 minutes and COR was 336% higher after SAL than after ATI at 120 minutes; at 30, 60, and 120 minutes, T was 4–10% higher after ATI than SAL. There were no other significant differences. REC, SED, and ALG were antagonized after ATI. ATI did not antagonize the effect of MED on HR, RR, or MAP, but stabilized T and antagonized the increase in GLU and COR.     

Leptin concentrations in periparturient ewes and their subsequent offspring

Leptin is an adipocyte-derived hormone that suppresses feed intake and increases energy expenditure. Leptin is also involved in regulating body temperature. Thus, the presence of leptin in milk, which can be absorbed through the gut of neonates immediately after birth, may aid in the survival of neonates born in cold weather. Our objectives were to determine the temporal relationship between concentrations of leptin in postpartum ewe blood serum and ewe milk serum, and to determine whether ewe blood and milk serum leptin concentrations were correlated with concentrations of leptin in lamb blood serum in their off-spring. Approximately 1 wk before the expected date of lambing, blood samples, weights, and body condition scores (BCS; 0 to 5 scale) were collected from 27 mixed-parity ewes. Following parturition, ewe blood and milk samples were collected within 2 h of parturition (d 0), 12 h (d 0.5) and 24 h (d 1) after parturition, again on d 5, and weekly thereafter until d 47. Lambs were blood-sampled and weighed within 2 h of parturition (d 0), bled daily until d 5, and bled and weighed weekly thereafter to d 47. Prior to lambing, ewe blood serum leptin was positively correlated with congruent BCS (r2 = 0, 10, P = 0.06), but not weight (P = 0.14). Following parturition, ewe blood serum leptin was positively correlated with BCS, weight, and milk serum leptin (r2 = 0.14, P < 0.0001, r2 = 0.12, P < 0.0001, and r2 = 0.028, P = 0.04). Leptin in milk serum was correlated with ewe weight (r2=0.05, P = 0.007) but not ewe BCS (P = 0.7); however, concentrations of leptin in both ewe blood and milk serum varied with day of lactation (P = 0.0001), being maximal within 24 h of parturition and declining to nadir concentrations by d 5. Leptin in lamb serum was correlated with milk serum leptin, (r2 = -0.05; P = 0.001), but not ewe blood serum leptin (P = 0.5). Concentrations of leptin in lamb serum increased from birth to d 5 and declined thereafter to nadir concentrations by d 19. Elevated concentrations of leptin in milk during the early stages of lactation may provide a mechanism for thermoregulation, satiation, and homeostatic endocrine control in the neonate.     

The influence of lime and nitrogen fertilisers on spore counts of Pithomyces chartarum in pasture

AIMS: To determine whether the application of lime or nitrogen to pasture affected the spore counts of Pithomyces chartarum.

MATERIALS AND METHODS: The lime application studies were undertaken on a spring-calving, pasture-based, commercial dairy farm near Te Awamutu, New Zealand. On 6 November 2012, five randomly selected paddocks were split into three equal sections. In two of the sections, lime was applied at either 1.5 or 2.5?t/ha, and the central section was left as an untreated control. Each section was sampled for spore counting weekly from 16 January to 15 May 2013.

Starting in January 2013, five other randomly selected paddocks were monitored for spore counts. On 20 March 2013 the average spore counts in three paddocks were >100,000 spores/g of pasture. These paddocks were then divided into three equal sections and lime was applied as described above. Spore counting in each section continued weekly until 15 May 2013.

The nitrogen application study was carried out on three commercial dairy farms near Te Awamutu, New Zealand. Two randomly selected paddocks on each farm were divided into three equal sections and, on 20 December 2012, nitrogen in the form of urea was applied at either 50 or 80?kg urea/ha to two of the sections; the central section remained as an untreated control. Each section was sampled for spore counting weekly from 16 January to 15 May 2013.

RESULTS: Following pre-summer lime application, treatment at 1.5 or 2.5?t/ha did not affect spore counts over time compared with the control section (p>0.26). Similarly following autumn lime application, treatment at 1.5 or 2.5?t/ha did not affect spore counts over time compared with the control section (p>0.11). Following nitrogen application median spore counts remained <20,000 spores/g pasture throughout the trial period and there was no effect of treatment on spore counts over time (p>0.49).

CONCLUSION: This study found that application of lime before the risk period for facial eczema, in November, application of lime after a spore count rise, in March, or urea application in December did not affect changes in number of spores produced by P. chartarum.

CLINICAL RELEVANCE: This study does not support previous suggestions that fertilising pasture with lime or urea could alter the spore counts of P. chartarum. Fertiliser use does not provide an alternative to, or support, conventional methods of facial eczema control such as zinc prophylaxis or treatment of pasture with fungicides.     

Studies on the biology of black mould (Aspergillus niger) on temperate and tropical onions. 2. The effect of treatments on the control of seedborne A. niger

Treatment of A. niger -inoculated onion seed with benomyl dust (1 g a.i./kg), or a foliar spray of thiram (0·4% a.i./ha) applied to plants grown from inoculated seed under temperate (UK) conditions reduced the incidence of A. niger in harvested crops. Treatment of naturally contaminated Sudanese onion seed with a benomyl + thiram mixture at a rate of 2·5 + 2·5 g a.i./kg or soaking the seed in hot water (15 min at 60°C) reduced the incidence of black mould on bulbs grown in the Sudan in field soil that had not previously been used for onion production. The seed treatments were less effective in crops produced in fields regularly used for onion production. However, incubation of harvested bulbs in moist chambers showed that damage to the internal storage tissue of onion bulbs caused by A. niger was reduced by seed treatment.     

Evolution des hydrates de carbone non structuraux chez la morelle jaune (Solatium elaeagnifolium Cav.)

Les teneurs en hydrates de carbone non structuraux (TNC) des racines, collets et tiges des in-dividus de la morelle jaune (Solarium elaeagnifolium Cav.) issue des bordures, champ de cotonnier et verger d'oliviers ont été déter-minées mensuellement pendant une année. Les racines et, dans une certaine mesure, les collets ont été les organes où les réserves glucidiques étaient les plus importantes. Ces glucides ont atteint leur niveau le plus bas au moment de la formation des boutons floraux. La reconstitution des réserves en TNC a lieu entre la floraison et la maturation des fruits sur une durée de 2, 4 et 5 mois pour les individus issus du champ de cotonnier, des bordures et du verger d'oliviers, respec-tivement. L'évolution des TNC est plus influencée par le stade phénologique de l'espèce que par rhumidité et la température du sol. Les implications de ces réquitals pour définir une stratégic de lutte centre la morelle jaune sont également discutées. Seasonal changes in non-structural carbohydrates in silver-leaf nightshade (Solanum elaeagnifolium Cav.) The levels of non-structural carbohydrates (TNC) in the roots, root collar and stems of S. elaeagnifolium collected from field margins, cotton fields and olive plantations were determined monthly over a period of 1 year. The roots, and to a certain extent the root collar, were the organs with the largest carbohydrate reserves. The carbohydrate level was lowest at the time of flower bud formation. There was a build-up of TNC reserves between flowering and fruit maturation over periods of 2, 4 and 5 months from plants growing in cotton fields, field margins and olive plantations respectively. Changes in TNC are influenced more by the phenological stage of the plant than by soil humidity or temperature. The implications of these findings in defining a control strategy for this weed are discussed. Saisonale Einlagerung von Kohlenwasserstoffen beim Ölweidenblättrigen Nachtschatten (Solanum eleagnifolium Cav.) Die Gehalte unstrukturierler Kohlenwasser-stoffe (TNC) des Wurzeln, des Wurzelhalses und des Sprosses von Solanum elaeagnifolium Cav. von Feldrainen, Baumwollfeldern und Olivengärten wurden monatlich über l Jahr hin bestimmt. Die Wurzeln und in bestimmten Maβe der Wurzelhals waren die Pflanzenteile, in denen die Kohlenwasserstoffe die wichtigsten Re-servesubstanzen waren; sie hatten ihr nie-drigstes Niveau zur Blütenbildung. Der Wiederaufbau dieser Reservestoffe zwischen der Blüte und der Fruchtreife dauerte bei den Pflanzen auf den Baumwollfeldern, den Feldrainen und den Olivengärten 2, 4 bzw. 5 Monate. Die Kohlenhydratbildung wurde mehrnach den Entwicklungsstadienals durch Boden feuchte und-temperatur beeinflußt. Die Aspekte dieserUntersuchungsergebnisse für die Bekämpfung dieses Unkrauts werden diskutiert.     

Morphological,Physiological, and Biochemical Responses to Water Stress in Melon (Cucumis melo) Subjected to Regulated Deficit Irrigation (RDI) and Partial Rootzone Drying (PRD)

Journal of Crop Science and Biotechnology - In this study, the impact of two water-saving techniques, Regulated Deficit Irrigation (RDI) and Partial Rootzone Drying (PRD) was investigated by...     

Demonstration of salmon farming as a net producer of fish protein and oil

To date aquaculture’s reliance on dietary marine sources has been calculated on a fish weight‐to‐weight basis without considering the absolute amounts of nutrients but this approach neglects the often considerable differences in the nutritional value of fish. We propose simple nutrient‐to‐nutrient‐based dependency measures that take into account these nutritional differences. In the first study reported here, individually tagged Atlantic salmon (Salmo salar) were reared in seawater supplied tanks with feed collection facilities. In the second, commercial net pens were used to grow over 200 000 fish. For both studies, a low marine ingredient feed containing approximately 165 g kg^?1 fishmeal was compared to a control feed (approx 300 g kg^?1 fishmeal) whilst fish oil inclusion was less markedly reduced. The low marine feeds supported similar growth and feed efficiency compared to the control feeds. With the low marine ingredient feeds, the weight of salmon protein and lipid produced through growth exceeded the weight of marine protein and lipid consumed by the fish meaning that salmon farming can be a net producer of fish protein and oil. The amount of n‐3 long‐chain polyunsaturated fatty acids deposited was sufficient to meet current recommendations from human health organizations.     

Intermediate Filament Proteins Expression and Carbohydrate Moieties in Trophoblast and Decidual Cells of Mature Cat Placenta

The aim of this study was to characterize cytoskeletal intermediate filament proteins and glycoconjugates of syncytiotrophoblast, cytotrophoblast and decidual cells of feline endotheliochorial placenta. Samples from 12 normal pregnant female cats, after 45 ± 5 days of gestation, were obtained removing the uterine horns by hysterectomy. Sections were processed for routine observation and for immunohistochemistry using anticytokeratin, antivimentin and antidesmin antibodies. In addition, lectin histochemistry was performed using a panel of several biotinylated lectins to characterize glycosides expression profile. Cytotrophoblast and syncytiotrophoblast showed immunoreactivity only with acidic and basic cytokeratins. Decidual cells were only positive to vimentin, consistent with their origin from endometrial fibroblasts. Trophoblast expressed a broad population of glycans, highly exposing terminal N‐acetyl glucosamine residues and non‐sialylated galactose and N‐acetyl galactosamine oligomers. Oligosaccharides bound by Phaseolus vulgaris erythroagglutinin were the only highly branched N‐linked residues evidenced in cats, and they were restricted to the syncytium. Unlike results reported on humans, mice and rats on lectin affinity of decidual cells, sialid acids and complex N‐linked oligosaccharides were not demonstrated in cats. Glycosylation of proteins determines many of their final properties, thus becoming essential for the embryo‐maternal dialogue during implantation and placentation. Changes in glycosylation pattern have been related to pathological pregnancies in other species. Hence, the knowledge about glycosylation profile of the normal cat placenta may lead to a better understanding of both normal and pathological reproductive events.     

Endometrial nitric oxide synthase activity in mares susceptible or resistant to persistent breeding‐induced endometritis and the effect of a specific iNOS inhibitor in vitro

Emerging research suggests that the nitric oxide system may play a role in persistent breeding‐induced endometritis (PBIE) in the mare. Differences in uterine nitric oxide (NO) levels between mares susceptible or resistant to PBIE and a dose‐dependent inhibitory effect of NO on uterine contractility have been demonstrated. The objectives of this study were to investigate the difference in total nitric oxide synthase (NOS) activity of the endometrium between susceptible and resistant mares and the effect of a specific inducible nitric oxide synthase (iNOS) inhibitor on the endometrial NOS activity in vitro. Six susceptible and six resistant mares were selected based on preset criteria and the results of an intrauterine challenge with killed spermatozoa during oestrus. Endometrial biopsy samples were collected 24 hr post‐challenge and cultured at 37°C for 24 hr in L‐arginine supplemented minimum essential medium with or without a specific iNOS inhibitor (1,400 W dihydrochloride, 1 mM). The medium and the cultured endometrial tissue were collected after 24 hr of culture and assayed for NO and total protein, respectively. Total NO content of the medium, normalized to endometrial tissue wet weight or total protein, was used as a measure of endometrial NOS activity. Non‐parametric tests were applied for statistical analysis. Susceptible mares had significantly greater endometrial NOS activity than resistant mares. The iNOS inhibitor treatment significantly reduced NOS activity in endometrial samples derived from susceptible and resistant mares. These findings provide a basis for in vivo testing of specific iNOS inhibitors as preventative or therapeutic options for PBIE in mares.