Declines of the gopher tortoise on protected lands

We compared two assessments of the status of gopher tortoise populations at 10 protected sites in Florida, taken about a decade apart. We assessed status indirectly, using surveys of burrows along belt transects. Transect placement and timing were identical between surveys. We compared numbers of burrows, relative numbers of burrows of different activity conditions, and size distributions of burrows between surveys. The comparisons indicated that populations had declined at as many as eight of the sites. We found no strong connection between population decline and decline in habitat quality, as reflected in decreased ground cover and/or increased canopy cover between surveys. The response of a population to decline in habitat quality may depend on initial habitat structure, the degree of change in habitat structure, the period of time over which change is measured, the amount of habitat involved, and the level of habitat management.     

Flea blood feeding patterns in cats treated with oral nitenpyram and the topical insecticides imidacloprid, fipronil and selamectin

A series of studies was conducted to determine the effect of systemically and topically active insecticides on blood consumption by fleas (Ctenocephalides felis). Infestations were conducted by placing fleas into plexi-glass chambers attached to the lateral rib cage of domestic short-hair cats. After pre-defined periods, fleas and flea feces were extracted using vacuum aspiration and spectrophotometrically analyzed for hemoglobin using Drabkin's reagent. To determine how rapidly nitenpyram kills actively feeding fleas, a single oral treatment was administered 24h after infestation. To determine the effect of nitenpyram on blood consumption of newly acquired fleas, cats were infested with fleas 1h post-treatment and fleas and flea feces from both studies were extracted at 15, 30, 60, 120, 240 and 480min post-treatment or post-infestation. To compare the effects of topically versus systemically active insecticides, 20 cats each with 2 chambers attached, were randomly allocated among groups and were infested with fleas 1h after each of 4 nitenpyram treatments, or at 7, 14, 21 and 28 days after a single application of commercial spot-on formulations of fipronil, imidacloprid or selamectin. Infestations were also completed for untreated (control) cats. Twenty-four hours after infestation, fleas and flea feces were removed for host blood quantification. If at any time, flea blood consumption in a treated group did not significantly differ from that of fleas infesting controls, that treatment group was withdrawn from the study. Nitenpyram effects on actively feeding fleas were first observed at 60min post-dosing when 38% of fleas were dead or moribund, and at 240min 100% were dead or moribund. Nitenpyram produced a significant reduction in flea blood consumption (p<0.05), which appeared to cease 15min after infestation. For the treatment comparisons, significantly more (p<0.05) blood was consumed by fleas taken from imidacloprid and fipronil-treated cats than from the nitenpyram or selamectin groups. Only on nitenpyram- or selamectin-treated cats were there significant reductions (p<0.05) in flea blood consumption on days 21 and 28, with significant difference (p>0.05) between these two groups on day 28. In this study systemically acting insecticides such as nitenpyram, and the topically applied but systemically active insecticide selamectin, were more effective in interfering with flea blood feeding than were imidacloprid and fipronil.     

Quantitatively tracing bacterial transport in saturated soil systems

Three isolates of Escherichia coli were labeled by their resistance to sodium azide and, separately, to novobiocin, nalidixic acid, and tetracycline. The strains exhibited a high degree of persistence in the soil environment and were recoverable on strain specific media at levels within the 95% confidence interval of the numbers covered on nonselective media. The E. coli strains were subsequently used to evaluate the events which would occur when a septic tank drainfield became submerged in a perched water table and effluentborne bacteria escaped into the groundwater. Field experiments were conducted by introducing the strains into horizontal lines installed into the A, B, and C horizons of a soil profile and transport was evaluated by collecting groundwater samples from 5 rows of piezometers (sampling six separate depth zones/row) located downslope from the injection lines. The major portion of subsurface transport of the bacterial populations occurred in specific zones in the soil profile and at an apparent maximum velocity of 17.0 cm min^?1. The maximum bacterial density in the groundwater, observed at each sampling distance downslope, was used to produce a mathematical relationship which described the overall decrease in numbers of organisms with increased distance through the soil. The potential health hazards which could occur by the subsurface transport of fecal organisms in relation to these experiments are discussed.     

Simulation of putting-green soil water dynamics: Implications for turfgrass water use

Results from a field experiment examining soil water fate within U.S. Golf Association (USGA) putting greens were used to examine the validity of a water flow simulation model. The experiment used six different sandy root zones each with depths of 300 mm overlying a 100 mm thick gravel layer. Data collected over two growing seasons consisted of measured rainfall, irrigation, drainage volume, and soil water contents; and calculated turfgrass evapotranspiration (ET). Turfgrass rooting was measured at the end of each growing season, and water retention curve and saturated hydraulic conductivity measurements were conducted at the end of the study. For each root zone treatment, HYDRUS-2D (H2D) was calibrated using a subset of the experimental data and then validated by comparing observed and predicted water contents at 76, 152 and 229 mm depth and over both growing seasons. Model efficiency (E) ranged from 0.33 to 0.78; Mean Absolute Error (MAE) ranged from 0.012 to 0.024 m³ m⁻³; and Root Mean Square Error (RMSE) ranged from 0.015 to 0.028 m³ m⁻³, for the six treatments and both years. Also, RMSE values were at best slightly larger than and at worst twice as large as the mean standard deviation values of replicate measurements. Thus, H2D simulation performed reasonably well in describing the water content results of the field study. The calibration results provide evidence of hysteresis in water retention where water retention properties from the field appear to follow the sorption or wetting curve as compared with the laboratory measurements following the desorption or drying curve. This suggests that standard laboratory measurements of water release would not precisely predict water retention behavior in the field and over estimate water storage of these capillary barrier soils. The validation results provide evidence for turfgrass use of perched water held within these profiles, even though turfgrass rooting is shallow and water storage principally occurs deep within the root zone. Thus, the perched water of USGA putting greens should serve reasonably well as a water reservoir for subsequent turfgrass use, allowing for water conserving irrigation practices that makes use of this stored water.     

Use of mammary gland and colostral characteristics for prediction of colostral IgG1 concentration and intramammary infection in Holstein cows.

OBJECTIVE: To determine whether mammary gland or colostral characteristics at calving could be used to predict colostral immunoglobulin G1 (IgG1) concentration or intramammary infection (IMI) and whether leakage of colostrum affects IgG1 concentration. DESIGN: Prospective study. ANIMALS: 113 multiparous Holstein cows. PROCEDURE: Cows were examined within 3 hours of calving, and mammary gland and colostral characteristics, colostral volume, somatic cell count, and concentrations of IgG1, fat, and protein were determined. Bacteriologic culture of mammary secretions was performed approximately 14 and 7 days before calving and at calving. Associations of gland and colostral characteristics with colostral IgG1 concentration, colostral volume, and IMI were examined. RESULTS: Thick or thin colostrum had higher IgG1 concentration than colostrum of intermediate viscosity. Colostrum from mammary glands that were firm had low IgG1 concentration. Colostral IgG1 concentration was weakly correlated with volume. Intramammary infection was likely to be detected if colostrum contained clots or blood or if the California Mastitis Test (CMT) score was > or = 2. Somatic cell count was higher for glands with IMI than for uninfected glands, and CMT score was correlated with cell count. CLINICAL IMPLICATIONS: Mammary gland and colostral characteristics were of little value in predicting IgG1 concentration. Our findings do not support recommendations that first milking colostrum that is thin (watery) or that is from cows producing large volumes not be fed to dairy calves. Colostral characteristics, particularly CMT score, were of value for predicting IMI.     

Diabetes mellitus associated with bilateral granulosa cell tumors in a mare

Both ovaries in a 10-year-old American Saddle Horse mare were determined to be larger than normal. The mare had problems with infertility during the preceding 3 years. Clinical evaluation revealed that the mare was polyuric, polydypsic, hyperinsulinemic, and hyperglycemic, and had reduced tolerance to exogenous glucose. Bilateral granulosa cell tumors were identified on histologic examination of the ovaries. The precise mechanism for the secondary diabetes mellitus was not determined; however, the cause was suspected to be a result of the synthesis and release of a hormonal substance by the tumor that affected carbohydrate metabolism and resulted in decreased peripheral sensitivity to insulin.