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71.
SD JOHNSTON D. BLYDE J. GAMBLE D. HIGGINS H. FIELD J. COOPER 《Australian veterinary journal》1997,75(9):648-651
Objectives To evaluate electro-ejaculation of free-range eastern grey kangaroos in the field and assess the efficacy of four diluents to preserve sperm motility over a 48-h period at 5°C.
Procedure and design Under gaseous anaesthesia, 25 free-range kangaroos were electro-ejaculated and characteristics of the ejaculate noted. Spermatozoa obtained from eight ejaculates were diluted in phosphate buffered saline containing various combinations of egg yolk and glucose and refrigerated at 5°C for 48 h.
Results Spermatozoa were recovered from 24 of 28 ejaculates. Mean (± SEM) semen volume (mL) and pH were 25.0 ± 1.9 and 7.1 ± 0.1 respectively. The forward motility (%), rate of movement of sperm (0 to 5) and sperm concentration (x 106 /mL) were 77.4 ± 1.5, 3.8 ± 0.9 and 31.2 ± 7.3 respectively. There was no significant difference between the four diluents in their ability to maintain forward motility of spermatozoa over 48 h. However, rate of movement over the same period was significantly (P < 0.01) improved when sperm were diluted in phosphate buffered saline containing 10% egg yolk.
Conclusions Electro-ejaculation is a safe and reliable method for collecting semen from free-ranging eastern grey kangaroos. Preliminary attempts at short-term preservation showed that the motility of kangaroo spermatozoa could be adequately stored for 24 h and that the addition of egg yolk to the semen diluent was beneficial for improving the rate of sperm movement. 相似文献
Procedure and design Under gaseous anaesthesia, 25 free-range kangaroos were electro-ejaculated and characteristics of the ejaculate noted. Spermatozoa obtained from eight ejaculates were diluted in phosphate buffered saline containing various combinations of egg yolk and glucose and refrigerated at 5°C for 48 h.
Results Spermatozoa were recovered from 24 of 28 ejaculates. Mean (± SEM) semen volume (mL) and pH were 25.0 ± 1.9 and 7.1 ± 0.1 respectively. The forward motility (%), rate of movement of sperm (0 to 5) and sperm concentration (x 10
Conclusions Electro-ejaculation is a safe and reliable method for collecting semen from free-ranging eastern grey kangaroos. Preliminary attempts at short-term preservation showed that the motility of kangaroo spermatozoa could be adequately stored for 24 h and that the addition of egg yolk to the semen diluent was beneficial for improving the rate of sperm movement. 相似文献
72.
73.
74.
Potassium, protein and basic amino acid relationships in swine 总被引:3,自引:0,他引:3
75.
76.
Parsons SD Cooper D McCall AJ McCall WA Streicher EM le Maitre NC Müller A Gey van Pittius NC Warren RM van Helden PD 《Veterinary immunology and immunopathology》2011,142(1-2):113-118
African buffaloes (Syncerus caffer) are the most significant wildlife maintenance hosts of Mycobacterium bovis, the causative organism of bovine tuberculosis (BTB). Current diagnostic tests for the detection of M. bovis infection in free-ranging buffaloes have numerous limitations and we wished to evaluate a modification to a human TB assay, the QuantiFERON-TB Gold (In-Tube) assay (QFT), as a practical diagnostic test for BTB in buffaloes. One hundred and seventy-five buffaloes were tested using the single intradermal comparative tuberculin test (SICTT) and a modified QFT (mQFT). An appropriate cut-off point for the mQFT was derived from SICTT results using receiver operator characteristic curve analysis. Twenty-six SICTT-positive buffaloes were killed and subjected to necropsy, and selected tissues were processed for mycobacterial culture and speciation. An optimal cut-off point for the mQFT was calculated as 66pg/ml. The assay correctly detected 39/40 SICTT-positive buffaloes and 129/134 TST-negative buffaloes and M. bovis was cultured from 21/26 slaughtered SICTT/mQFT-positive animals. The mQFT shows promise as a practical test for M. bovis infection in buffaloes and shows a sensitivity and specificity at least similar to that of the TST. 相似文献
77.
Kramer L Grandi G Passeri B Gianelli P Genchi M Dzimianski MT Supakorndej P Mansour AM Supakorndej N McCall SD McCall JW 《Veterinary parasitology》2011,176(4):357-360
Adulticide therapy in heartworm (Dirofilaria immitis)-infected dogs can lead to thromboembolism, which can seriously compromise post-treatment health status. Lung pathology following adulticide therapy was evaluated in three groups of experimentally infected dogs. Group 1 was treated with doxycycline at 20 mg/kg per os once daily for 30 days post infection followed by an intramuscular injection of melarsomine dihydrochloride (2.5 mg/kg) at Week 12, followed 1 month later by two injections 24 h apart. Group 2 was treated as described for Group 1, with the addition of ivermectin at 6 mcg/kg given monthly per os for 24 weeks post-infection. Group 3 received melarsomine alone, as described above. All dogs were necropsied at Week 24 and lung pathology was evaluated. Lesion criteria included perivascular inflammation and endothelial proliferation. Lesions were scored by two independent pathologists who were blinded as to treatment. Results indicate that doxycycline treatment alone or combined with ivermectin had lower lesion scores than lungs from dogs who had received melarsomine alone. Dogs that received the combined doxycycline/ivermectin protocol and treated with adulticide showed less severe arterial lesions and the virtual absence of thrombi. 相似文献
78.
Maia FC McCall JW Jr VA Peixoto CA Supakorndej P Supakorndej N Alves LC 《Veterinary parasitology》2011,176(4):304-312
Clinical signs are seldom observed in feline heartworm disease, and the pathophysiological changes in the lungs of infected animals remain undefined. The goal of this study was to evaluate the structural and ultrastructural changes in the lungs of cats experimentally infected with Dirofilaria immitis. Six healthy cats were each infected with two adult heartworms by intravenous transplantation (Receptor Group, RG). The control group consisted of two uninfected animals kept under the same conditions as the RG. At 42 days after transplantation, all cats were euthanized and necropsied for worm recovery and collection of lung samples for examination by light microscopy (LM) and transmission electron microscopy. By LM, lung sections from the six infected cats exhibited bronchial and bronchiolar lesions. Alterations in all tissues of the pulmonary arteries were observed in the infected animals. In conclusion, cats infected experimentally with D. immitis developed lesions in their lungs as a consequence of arterial disease and intense interstitial pneumonia. 相似文献
79.
80.
R B McCall 《Science (New York, N.Y.)》1970,170(958):644-648
Comparisons between sibling and parent-child pairs with unrelated control pairs matched for year of birth and parental education were made to determine the relative heritability of the general level of intelligence quotient as opposed to that of the sequential pattern of IQ change over age (3 to 12 years). There was greater similarity among related siblings relative to matched controls for general level than for pattern of IQ over age. Relationships between the IQ's of children and that of their parents as children were not consistent across age. 相似文献