Genetics of resistance to Sesamia inferens infestation and its correlation with yield in maize

Sesamia inferens (Walker) causes 25.7–78.9% losses in maize production in South and South‐East Asia. The genetic basis of host plant resistance is the prerequisite for resistance breeding. Twenty‐four populations derived from S. inferens resistant × susceptible inbreds were used to study the genetic regulation of resistance to S. inferens in maize, to determine the importance of genetic effects through generation mean analysis (GMA) and to understand correlation between resistance and yield. Resistant and susceptible inbreds differed significantly in leaf and stem injury ratings (LSIR), a measure of host plant resistance. Mean LSIR range among resistant and susceptible parents was 2.15–2.55 and 7.83–8.22, respectively. The broad‐sense heritability ranged from 0.40 to 0.71, and the mean number of effective factors ranged from 1.9 to 2.6. The resistance against S. inferens was largely governed by additive × additive (i), followed by dominance (d) and additive (a) gene effects. Significant negative correlation (?0.27 to ?0.96) was observed between LSIR and yield. The findings suggest reciprocal recurrent selection for development of new inbreds with resistance followed by their involvement in hybrid development to exploit additive and non‐additive gene effects/variance.     

Biocontrol of soil diseases and soil profile associated with rhizosphere of rice (Oryza sativa Subsp. Japonica) growing paddy fields in Kansai region,Japan

ABSTRACT

This research focused on in vitro and in vivo biocontrol of two major rice diseases, Bacterial Leaf Blight caused by Xanthomonus oryzae pv oryzae (Xoo) and Sheath Blight caused by Rhizoctonia solani. Furthermore, the soil of paddy fields in Kansai region, Japan was analysed for the presence of these pathogens, and their microbial communities were evaluated according to the location and management systems. For biocontrol, the rhizospheric bacteria were isolated and molecularly identified. The results indicated that DAR17225040 and DAR17225017 were 99% similar to Bacillus aryabhattai and Bacillus megaterium. The isolated bacteria were able to suppress Bacterial Leaf Blight and Sheath Blight disease 78% and 86% respectively, under greenhouse conditions. The 16S amplicon sequencing (Illumina MiSeq 250bp) was used to analyse the bacterial community in paddy fields of soil taken from five locations under conventional and organic systems. Xoo and R. solani were detected in sampled soil, however, no clear difference was noted, regarding systems. From amplicon sequencing data, Chloroflexi, Acidobacteria, Proteobacteria, Actinobacteria, Planctomycetes were the dominant phyla of rice soil in Kansai region. The phylum Kazan-3B-28 was found more in organic system than conventional. The bacterial community was more affected by location and soil type than by management systems.     

Prevalence of Dirofilaria immitis infection in living raccoon dogs assessed by hematological examination

The prevalence of Dirofilaria immitis in free-ranging raccoon dogs (Nyctereutes procyonoides viverrinus) was examined in the southeast region of Kanagawa Prefecture, Japan, using a rapid immunomigration (RIM) test kit. Between April 2007 and March 2010, we examined 108 raccoon dogs rescued and housed by the Kanazawa Zoological Garden. D. immitis infection was found in 8 (7.4%) raccoon dogs. This is the first report to reveal the prevalence of D. immitis infection in living raccoon dogs. The prevalence of the infection was lower than previously reported values obtained on postmortem examination. One reason might be that the present study included young raccoon dogs infected with immature worms. Significant high-risk areas of D. immitis infection in the raccoon dogs were not observed.     

Fracture propagation to the base of the Greenland Ice Sheet during supraglacial lake drainage

Surface meltwater that reaches the base of an ice sheet creates a mechanism for the rapid response of ice flow to climate change. The process whereby such a pathway is created through thick, cold ice has not, however, been previously observed. We describe the rapid (<2 hours) drainage of a large supraglacial lake down 980 meters through to the bed of the Greenland Ice Sheet initiated by water-driven fracture propagation evolving into moulin flow. Drainage coincided with increased seismicity, transient acceleration, ice-sheet uplift, and horizontal displacement. Subsidence and deceleration occurred over the subsequent 24 hours. The short-lived dynamic response suggests that an efficient drainage system dispersed the meltwater subglacially. The integrated effect of multiple lake drainages could explain the observed net regional summer ice speedup.     

Seismotectonics of mid-ocean ridge propagation in Hess Deep

Hydroacoustic data from the eastern equatorial Pacific reveal low-magnitude seismicity concentrated at the propagating tip of the Galapagos Rise in Hess Deep. The patterns of seismicity and faulting are similar to those observed in the process zone of laboratory-scale propagating tensile cracks. Because the fracture energy required for propagation scales with crack length and process zone size, it follows that ridges can propagate stably in the brittle crust without exceptional resisting forces as proposed by previous models based on linear elastic fracture mechanics.     

Association of staygreen trait with canopy temperature depression and yield traits under terminal heat stress in wheat (Triticum aestivum L.)

The presence or absence of the staygreen trait was screened for 3 consecutive years in 963 wheat lines from various sources, including Indian and CIMMYT germplasm. Staygreen was assessed at the late dough stage by visual scoring (0–9 scale) and the leaf area under greenness (LAUG) measurement. Around 5.5 % of the lines were staygreen, 10.5 % were moderately staygreen, and the remaining lines showed little or no expression of the trait. One hundred lines showing diversity for the staygreen trait were sown under three different sowing dates (timely, late and very late) for 3 consecutive years in three replications to determine the association of staygreen with heat tolerance. There was a decline in yield, biomass, grain filling duration (GFD) and 1,000 grain weight (TGW) under late and very late sowing conditions owing to terminal stress at anthesis and later stages. However, the decline was relatively less in staygreen genotypes compared to the non-staygreen (NSG) ones. The correlation study showed that LAUG and canopy temperature depression (CTD) were strongly correlated. LAUG and CTD were also significantly associated with grain yield, GFD and biomass. To further confirm the association of the staygreen trait with terminal heat stress, individual F₂-derived F₇ progenies from the cross of the ‘staygreen’ lines with NSG were evaluated for yield and yield traits at the three sowing dates. In each cross, the staygreen progenies showed a significantly smaller decline in yield and TGW under heat stress than the NSG progenies. These results appear to suggest an association between the staygreen trait and terminal heat stress and, thereby, that the staygreen trait could be used as a morphological marker in wheat to screen for heat tolerance.     

Sexing of Dog Sperm by Fluorescence In Situ Hybridization

Effective preselection of sex has been accomplished in several species of livestock and also in humans using the flow cytometric sperm sorting method. A guaranteed high sorting accuracy is a key prerequisite for the widespread use of sperm sexing. The standard validation method is flow cytometric remeasurement of the DNA content of the sexed sperm. Since this method relies on the same instrument that produced the original sperm separation, it is not truly independent. Therefore, to be able to specifically produce either male or female offspring in the dog, we developed a method of direct visualization of sex chromosomes in a single sperm using fluorescence in situ hybridization (FISH) as a validation method. Denaturation of canine spermatozoa by immersion in 1 M NaOH for 4 min yielded consistent hybridization results with over 97% hybridization efficiency and a good preservation of sperm morphology. There was no significant difference between the theoretical ratio (50:50) and the observed ratio of X- and Y-chromosome-bearing spermatozoa in any of the three dogs. In addition, the mean purities of flow-sorted sex chromosomes in spermatozoa of the three dogs were 90.8% for the X chromosome fraction and 89.6% for the Y chromosome fraction. This sorting was evaluated by using the dual color FISH protocol. Therefore, our results demonstrated that the FISH protocol worked reliably for both unsorted and sexed sperm samples.     

Ethnobotanical flora used by four major tribes of Koraput, Odisha, India

The aim of this study is to chronicle the utilization pattern of a number of plant species by four major tribes inhabiting in Koraput district of Odisha, India. Fifteen villages dominated by tribal communities, under five Community Development Blocks were selected. A semi structured questionnaire was developed to bring together data on traditional crops and forest species still used by the communities. The methodology engaged was personal discussions with mixed focused groups combined with field visits, photographs, voice recordings and collection of materials. Findings were that tribal communities cultivate a diverse crop species with varying durations to meet their food and economic requirement throughout the year. This was supplemented by wild food collections during scarcity periods. Various forest species provide wood, fibre, leaves and grasses for agriculture, house construction and specific uses besides enhancing household income. Tribal health care practitioners collect medicinal plants from the wild and semi wild to treat both humans and livestock. At the end of the study the team assumed that tribal communities would continue to depend on traditional crops and wild forest foods for their household consumption and a diverse species of plants for other specific use. Conservation of these valuable resources in its natural habitat would be an appropriate approach for ensuring food security of future generations. Conventional plant breeders and biotechnologists should undertake scientific validation of these crops and plants to make their value explicitly known. This knowledge could be utilized for crop improvement and bio-prospecting. There is also scope for equitable sharing of benefits if used by the seed and pharmaceutical industries in future.     

Physiochemical and Textural Alterations in Indian Squid (Loligo duvauceli) Mantle During Frozen Storage and Cooking

Textural variations in squid mantle and the role of proteins on texture during frozen storage and cooking were investigated. Myofibrillar protein (62.36%) and pepsin soluble collagen (10.70%) accounted for the major fraction of total protein (22.17%). The histochemistry of mantle tissue showed a mesh-like arrangement of myofibrillar proteins with a collagenous dermal layer and feebly passing collagen through myotome bundles. Texture profile analysis of unfrozen mantle suggested the first phase of hardening at 50°C with hardness 1 (H1) of 11.53 kgf and hardness 2 (H2) of 9.68 kgf; and the second phase of hardening with optimal texture and maximum juiciness at 70°C (H1, 8.11 kgf; H2, 7.13 kgf) that varied with extended frozen storage. Increased frozen storage and cooking led to protein denaturation and formation of new low molecular weight proteins as evidenced in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE); these possibly influenced the functional and microstructural properties of the tissue.