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31.
Integrating principles of ecological intensification into weed management strategies requires an understanding of the many relationships among weeds, crops and other organisms of agro‐ecosystems in a changing context. Extensively used during the last two decades in weed science, trait‐based approaches have provided general insights into weed community response to agricultural practices, and recently to understanding the effect of weeds on agro‐ecosystem functioning. In this review, we provide a holistic synthesis of the current knowledge on weed response and effect functional traits. Based on the literature and recent advances in weed science, we review current knowledge on (i) weed functional groups and ecological strategies, (ii) weed functional response traits to cropping systems and (iii) weed functional effect traits affecting agro‐ecosystem functioning. For each functional trait, we explicitly present the assumptions and evidence on the linkage between trait values and ecological functions, in response to either management practices, for example tillage, sowing and herbicides, or biotic interactions, for example crop–weed competition and pollination. Finally, we address and discuss major research avenues that may significantly improve the use of traits and the knowledge of functional diversity in weed science for the future, especially to design and implement more environmentally sustainable weed management strategies.  相似文献   
32.
We investigated whether enhanced nitrogen (N) and water inputs would redistribute the microbial community within different soil aggregate size classes in a field manipulation experiment initiated in 2005. Distribution of microbial groups was monitored in large macroaggregates (>2000 μm), small macroaggregates (250–2000 μm), and microaggregates (<250 μm) in a semiarid grassland. Both arbuscular mycorrhizal (AM) fungi and saprophytic fungi were the most abundant in soil macroaggregates. The gram-negative bacteria were more abundant in soil microaggregates. Total phospholipid fatty acid (PLFA) concentration in general and actinomycetes in particular decreased with N addition under ambient precipitation but was unaffected by combined additions of N and water within the three soil aggregate fractions as compared to control plots. In contrast, the abundance of saprophytic fungi decreased with combined N and water addition, but it was not affected by N addition under ambient precipitation. The abundance of gram-positive bacteria increased with N addition under both ambient and elevated water conditions for all soil aggregate fractions. In summary, the higher short-term nutrient and water availabilities provoked a shift in soil microbial community composition and increased total PLFA abundance irrespectively of the level of soil aggregation. In the long term, this could destabilize soil carbon pools and influence the nutrient limitation of soil biota within different soil aggregate size classes under future global change scenarios.  相似文献   
33.
Grain compositional components impacting barley (Hordeum vulgare L.) use in food, feed and fuel products, must be combined with improved agronomic traits to produce a commercially viable barley cultivar. Little current information is available on grain composition and variability among winter barley genotypes. This study was conducted to determine the variability among modern hulled and hulless winter barley genotypes in grain composition. Barley types varied significantly in grain and kernel weight, starch, beta-glucan, oil and ash content, but not in protein concentration. Hulless barley had significantly higher grain test weight and starch concentration than hulled and malting types, and significantly higher beta-glucan than malting barley. Hulless barley had significantly lower kernel weights, oil, and ash concentrations than hulled and malting types. Higher starch and lower fiber and ash in grain of hulless barley versus hulled feed or malting type barley are characteristics that increase hulless barley desirability. Selection for high starch concentration among all barley types is feasible and will facilitate development of barley cultivars better suited for use in feed, malt, and ethanol production.  相似文献   
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35.
AIM: To establish a one-stage model of experimental acute necrotizing pancreatitis (ANP) in rats characterized by the simplicity of performance and a high degree of repeatability. METHODS: ANP modeling in rats was performed based on modification of the ligation model as follows: synthetic material ligature using an atraumatic needle was performed to capture pancreatic gland ducts and marginal duodenum vessels. Ligature tips were exteriorized to the abdominal wall, and the ligature was skinned over to avoid catching intestine loops. Pancreatic macroscopic appearance and histological changes were observed. Blood biochemical and hemostatic indicators were also determined. RESULTS: Laboratory analysis of rats with experimental ANP showed a pattern of disturbances similar to that observed during pancreatic necrosis in humans as soon as the first day. General blood analysis revealed enhanced leukocytosis and alterations in leukogram characteristics, indicating acute inflammation. Serum levels of amylase, aspartate aminotransferase and creatinine significantly increased (P<0.05). Hemostatic indicators showed alterations indicating formation of disseminated intravascular coagulation, and signs of endotoxicosis were observed. These typical pancreatic necrosis patterns of disturbances were validated by the results of histological investigation. CONCLUSION: Histological changes and laboratory indicators confirm the development of a suitable model of ANP.  相似文献   
36.
Widespread epidemics of Stenocarpella ear rot (formerly Diplodia ear rot) have occurred throughout the central U.S. Corn Belt in recent years, but the influence of S. maydis infected grain on corn ethanol production is unknown. In this study, S. maydis infected ears of variety Heritage 4646 were hand‐harvested in 2010 from a production field in central Illinois and segregated into one of five levels of ear rot severity based upon visual symptoms. The concentration of ergosterol, a sterol produced by fungi but not plants, was observed to increase with the severity of ear rot (127–306.5 μg/g), and none was detected in the control corn. Corn test weight declined with progression of the disease and was 42.6% lower for the most severely rotted grain from ears infected early in their development. Accompanying changes in composition were also apparent. Crude fat and oil contents decreased (from 4.7 to 1.5%) and fiber increased (from 6.6 to 9.6%), but starch content remained largely invariant. Oil composition also varied among the infected samples. Control and infected corn samples were subjected to ethanol fermentation with a laboratory‐scale corn dry‐grind ethanol process. Ethanol yields for control and infected samples were similar on an equivalent weight basis (2.77–2.85 gal/bu). In comparison with the control, S. maydis infection altered the distillers dried grains with solubles (DDGS) properties, wherein the crude protein was significantly higher and oil significantly reduced, and ash, fiber, and yield per ton were not significantly different. Based upon these results, we conclude that Stenocarpella ear rot has the potential to affect DDGS composition but not ethanol yield on an equivalent weight basis.  相似文献   
37.
Mucopolysaccharidosis (MPS) types I and VII are inborn errors of metabolism caused by mutation of enzymes involved in glycosaminoglycan catabolism, which leads to intralysosomal accumulation of glycosaminoglycans. In children, severe forms of MPS I and VII are characterized by somatic and neurologic manifestations, including a poorly understood hearing loss. The purpose of this study is to describe the age-related histopathologic changes of the ear in spontaneous canine models of MPS I and VII. Pathologic changes in the ear were assessed in MPS I and VII dogs ranging from 1.6 to 9.3 months of age. Paraffin-embedded sections of the whole ear and Epon-embedded semithin sections of the cochlea were examined. The following lesions were blindly scored in the middle and inner ear: inflammation, cells vacuolization, thickening of osseous and membranous structures, perivascular vacuolated macrophages infiltration, and bone resorption. All dogs had lysosomal storage within cells of tympanic membrane, ossicles, tympanic bone and mucosa, cochlear bone, spiral ligament, limbus, and stria vascularis. The MPS I dogs mainly had progressive cochlear lesions. The MPS VII dogs had severe and early middle ear lesions, including chronic otitis media and bone resorption. The MPS I dog only partially recapitulates the pathology seen in humans; specifically, the dog model lacks inflammatory middle ear disease. In contrast, the MPS VII dog has severe inflammatory middle ear disease similar to that reported in the human. In conclusion, the canine MPS VII model appears to be a good model to study MPS VII-related deafness.  相似文献   
38.
The purpose of this study was to investigate the pharmacological effects of calcium alginate on carbon tetrachloride (CCL4)-induced hepatotoxicity in rats. The study included two experiments. In the first experiment the animals were given daily CCL4 through gavage for 7 days and then 10, 50, or 250 mg/kg b.w. of calcium alginate for 21 days. The increased bilirubin level, enhanced alanine and aspartate aminotransferase activity in plasma and reduced liver glycogen content induced by CCL4 were partly normalized by alginate administration in a dose-dependent manner. In addition, alginate significantly improved CCL4-induced alterations of pro-oxidant and antioxidant biochemical parameters in liver and plasma compared to those of rats administered CCL4. In the second experiment the animals were given daily 10, 50 or 250 mg/kg b.w. of calcium alginate for 21 days before 7-day administration of CCL4. Pretreatment with alginate before CCL4 administration resulted in significantly inhibited increase of the blood enzymatic activities of alanine and aspartate aminotransferases and bilirubin level in a dose-dependent manner. Also, preliminary administration of alginate prevented elevation of lipid peroxidation products and reduction of liver glutathione content in rats given CCL4. These results suggest that calcium alginate exerts healing and preventive effects on CCL4-induced hepatotoxicity in rats.  相似文献   
39.
Journal of Soils and Sediments - The aim of this study was to characterize the influence of soil contamination on the trace element accumulation in nodules, inter-element relationships inside the...  相似文献   
40.
Many studies already investigated marker-assisted selection (MAS) efficiency but mainly in biparental populations. Connected multiparental populations address a broader diversity and confer a gain of power for QTL detection which must be beneficial for MAS. Our objective was to compare multiparental connected designs to biparental populations taken separately for MAS and phenotypic selection. We first detected QTL for flowering time and grain yield in an experimental maize design involving four parental inbred lines crossed to produce six different biparental populations and confirmed the advantage of multiparental connected designs over biparental populations for QTL detection. Based on these results we performed stochastic simulations to evaluate the expected efficiency of four generations of MAS and phenotypic selection. Different parameters were considered: trait heritability, genetic architecture and whether QTL were assumed to be known or have to be detected. Genetic gains were higher in the multiparental design than on average over the biparental populations considered separately, especially when favourable alleles were equally distributed among parental lines. When QTL detection was included in the simulation process, we found that type I error risk considered for declaring QTL as significant should be adapted to the design. Type I error risks leading to the best response were higher for the biparental populations than for the multiparental design. Besides addressing a broader diversity, multiparental designs increase the power of QTL detection, which reinforces their superiority over biparental designs for MAS. Application of MAS to multiparental designs therefore appears promising to accelerate genetic gain in plant breeding programs.  相似文献   
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