Effect of incubation temperature on the embryonic development and yolk‐sac larvae of the Pacific red snapper Lutjanus peru (Nichols & Murphy, 1922)

The effect of incubation temperature on embryonic development and yolk‐sac larva of the Pacific red snapper Lutjanus peru were evaluated by testing the effect of 26, 28 and 30°C, as this is the natural thermal interval reported during the spawning season of Pacific red snapper in the Gulf of California, Mexico. Sixteen developmental stages were observed. The incubation temperature affected the rate of development and time to hatching, being shorter at 30 than at 26°C, but no significant effect (P < 0.05) on larval length at hatching was registered. The depletion rate of yolk sac and oil globule was affected by incubation temperature particularly during the first 12 h post hatching (hph). At the end of the experiment (48 hph), significantly (P < 0.05) larger larvae were recorded at 26°C (TL = 3.22 ± 0.01 mm) than at 28° (TL = 3.01 ± 0.02 mm) and 30°C (TL = 2.97 ± 0.05 mm). Incubation of newly fertilized eggs at 26°C produces larger larvae, which may help to improve feeding efficiency and survival during first feeding.     

Use of geophysical methods for the study of sandy soils under Campinarana at the National Park of Viruá, Roraima state,Brazilian Amazonia

Purpose

The vegetation of the Campinaranas occurs in humid areas with hydromorphic sandy soils at the Amazon region. Thus, the determination and in situ monitoring of moisture content in Campinarana soils, besides the detection of subsurface layers are key measures for studying these soil–vegetation systems. Also, the application of ground penetrating radar (GPR) in deep sandy sedimentary sequence of Amazonia is a promising tool to enhance the knowledge on depositional and soil formation features.

Materials and methods

We studied representative soils of the Campinaranas at the National Park of Viruá, state of Roraima (Brazilian Amazonia), through the use of geophysical methods (soil moisture sensors and GPR). The study was applied in four sandy soils. Besides chemical and physical analysis of soils, soil moisture sensors were installed for monitoring during an entire hydrological year (2010/2011), and performed the calibration of sensors , coupled with imaging of the soil along transects with GPR.

Results and discussion

As a result of calibration of the soil moisture sensors we obtained a general equation with an R ² greater than 0.9. There is an influence of soil moisture content and soil temperature in the distribution of vegetation types in Campinaranas. The use of GPR identified some determinants characteristics in these soils for the differentiating the Campinaranas, represented by spodic and C horizons.

Conclusions

The spodic horizons in soils under Forest Campinarana provided potential errors in the determination of soil moisture, requiring calibration data for the precise use of this device. The investigation of the soil through the GPR showed interesting results, which allowed continuous visualization of the main soil horizons along transects in the phytophysiognomies of Campinaranas.     

A kinetic model of phosphorus metabolism in growing goats

The effect of increasing phosphorus (P) intake on P utilization was investigated in balance experiments using 12 Saanen goats, 4 to 5 mo of age and weighing 20 to 30 kg. The goats were given similar diets with various concentrations of P, and 32P was injected to trace the movement of P in the body. A P metabolism model with four pools was developed to compute P exchanges in the system. The results showed that P absorption, bone resorption, and excretion of urinary P and endogenous and fecal P all play a part in the homeostatic control of P. Endogenous fecal output was positively correlated to P intake (P < .01). Bone resorption of P was not influenced by intake of P, and P recycling from tissues to the blood pool was lesser for low P intake. Endogenous P loss occurred even in animals fed an inadequate P diet, resulting in a negative P balance. The extrapolated minimum endogenous loss in feces was .067 g of P/d. The minimum P intake for maintenance in Saanen goats was calculated to be .61 g of P/d or .055 g of P/(kg(.75) x d) at 25 kg BW. Model outputs indicate greater P flow from the blood pool to the gut and vice versa as P intake increased. Intake of P did not significantly affect P flow from bone and soft tissue to blood. The kinetic model and regressions could be used to estimate P requirement and the fate of P in goats and could also be extrapolated to both sheep and cattle.     

Synergistic Interaction Between Tomato chlorosis virus and Tomato spotted wilt virus Results in Breakdown of Resistance in Tomato

ABSTRACT Multiple viral infections frequently are found in single plants of cultivated and wild hosts in nature, with unpredictable pathological consequences. Synergistic reactions were observed in mixed infections in tomato plants doubly infected with the positive-sense and phloem-limited single-stranded RNA (ssRNA) crinivirus Tomato chlorosis virus (ToCV) and the negative-sense ssRNA tospovirus Tomato spotted wilt virus (TSWV). Synergism in a tomato cultivar susceptible to both viruses resulted in a rapid death of plants. A pronounced enhancement of ToCV accumulation mediated by TSWV co-infection was observed with no evident egress of ToCV from phloem tissues. No consistent alteration of TSWV accumulation was detected. More remarkable was the synergism observed in tomato cultivars which carry the Sw-5 resistance gene, which are resistant to TSWV. Pre-infection with ToCV resulted in susceptibility to TSWV, whereas co-inoculations did not. This suggested that a threshold level or a time lapse is needed for ToCV to interfere or downregulate the defense response in the TSWV-resistant plants.     

Supervised independent component analysis as an alternative method for genomic selection in pigs

The objective of this work was to evaluate the efficiency of the supervised independent component regression (SICR) method for the estimation of genomic values and the SNP marker effects for boar taint and carcass traits in pigs. The methods were evaluated via the agreement between the predicted genetic values and the corrected phenotypes observed by cross‐validation. These values were also compared with other methods generally used for the same purposes, such as RR‐BLUP, SPCR, SPLS, ICR, PCR and PLS. The SICR method was found to have the most accurate prediction values.     

Understanding overbidding: using the neural circuitry of reward to design economic auctions

We take advantage of our knowledge of the neural circuitry of reward to investigate a puzzling economic phenomenon: Why do people overbid in auctions? Using functional magnetic resonance imaging (fMRI), we observed that the social competition inherent in an auction results in a more pronounced blood oxygen level-dependent (BOLD) response to loss in the striatum, with greater overbidding correlated with the magnitude of this response. Leveraging these neuroimaging results, we design a behavioral experiment that demonstrates that framing an experimental auction to emphasize loss increases overbidding. These results highlight a role for the contemplation of loss in understanding the tendency to bid "too high." Current economic theories suggest overbidding may result from either "joy of winning" or risk aversion. By combining neuroeconomic and behavioral economic techniques, we find that another factor, namely loss contemplation in a social context, may mediate overbidding in auctions.     

Retrospective and prospective studies of transmissible viral proventriculitis in broiler chickens in Brazil

We investigated the occurrence and pathologic findings of transmissible viral proventriculitis (TVP) associated with the chicken proventricular necrosis virus (CPNV) in commercial broiler chickens in southeastern Brazil. Seventy-three broilers, 25–36 d old, with a history of reduced growth, were referred to our veterinary pathology services from 2013 to 2017. Broilers were clinically examined, weighed, and euthanized for postmortem examination. Broilers of different ages with proventricular histologic lesions were positive for CPNV by RT-PCR; however, the intensity of histologic lesions was higher among 33-d-old animals, and viral RNA detection was more frequent among those that were 28 d old. In the proventriculi of 35 of 73 (48%) broilers, lesions were characterized by glandular epithelial necrosis, lymphoplasmacytic and histiocytic infiltrates, and metaplasia of glandular epithelium to ductal epithelium. In 24 of 73 (36%) broilers with histologic TVP-compatible lesions, CPNV was detected by RT-PCR for the viral protein 1 (VP1) gene. Broilers with histologic lesions were lighter than expected compared to the Cobb 500 standard weight. TVP has not been reported previously in broiler chickens in Brazil, to our knowledge.     

Inorganic Parameters as Water Quality Indicators in Acidic Groundwater in a Tropical Region – Brasilia-DF (Brazil)

In Brazilian Tropical Cerrado Region, reactions related to parental-material breakdown and oxisols genesis strongly affect the groundwater chemical characteristics. These reactions are responsible for the low water pH values, the soil charge balance, and the re-equilibrium of dissolved inorganic carbon species. Brasilia, the capital of Brazil, lies in the Distrito Federal, in the central part of the Brazilian Cerrado Region and has been experiencing an intense urban development during the last 15 years. Many town-house complexes have been built without proper water supply or wastewater disposal conditions. In this work, we discuss the adequacy of traditional ground waters geochemical classification models applied to the most exploited aquifers in DF. Forty groundwater samples were collected and pH, conductivity and total dissolved solids were determined in the field. Na, K, Ca, Mg, Fe, Al, Cu, Cd, Cr Mn e Zn were determined by Inductively Coupled Plasma Atomic Emission Spectrometry and Atomic Absorption Spectrophotometry. We identify inorganic indicators of human interference on the quality of these waters and also suggest a new mean of interpretation of dissolved inorganic elements based on statistic treatment of multivariate data. It is revealed a particular geochemical feature concerning to the alkali and the earth alkali metals concentrations, and a typical trace-element behaviour, closely related to the human activities and to the geological-bearing material characteristics. For all of the samples supposed to be altered by any kind of human activity, the physico-chemical parameters were lower than the standards established by the water quality legal system.