Derivation, safety and efficacy of a Marek's disease vaccine developed from an Australian isolate of very virulent Marek's disease virus

Objective To develop a serotype 1 Marek's disease (MD) vaccine from a very virulent MDV (vvMDV) pathotype and demonstrate safety and efficacy against early challenge with very virulent field strains in the presence of maternal antibody.
Study design Strain BH 16 was isolated and attenuated by serial cell culture passage. One of two cloned passages was selected for vaccine development following early laboratory-scale protection trials in commercial birds. Comparative protection trials were carried out on the BH 16 vaccine and on a CVI 988 Rispens vaccine using commercial and SPF chickens. Challenge viruses used were either a low passage strain BH 16 virus, the Woodlands No. 1 strain or MPF 57 strain of MDV. The BH 16 vaccine was back-passaged in SPF chickens six times and virus recovered from the final passage and the original vaccine virus were tested for safety. The immunosuppressive potential of the BH 16 and Rispens vaccines was also assessed in parallel.
Results The BH 16 and Rispens vaccines induced comparable levels of protection when used as monovalent or multi-valent vaccines, although protection achieved with the mono-valent vaccines was lower. No gross tumour formation was evident in any birds receiving the BH 16 vaccine or bird-passaged virus, although microscopic lesions were present in 2/12 birds that received the bird-passaged virus. In tests for immunosuppression, there was no histological evidence of damage to either the bursa of Fabricius or the thymus.
Conclusion The BH 16 vaccine was shown to be safe and at least as protective as the Rispens vaccine against three highly virulent MD challenge viruses.     

玉米的不同温度和时间烘烤加工处理对泌乳奶牛瘤胃干物质和淀粉降解规律的研究

以3头安装永久性瘤胃瘘管的泌乳前期荷斯坦奶牛为试验动物，在精粗比为55：45的日粮条件下，采用In sacco法测定了不同温度和时间烘烤加工处理的玉米在奶牛瘤胃内干物质和淀粉的降解规律。结果表明，玉米经过不同温度和时间的烘烤处理，干物质和淀粉的瘤胃降解率都有不同程度的降低，随着温度逐渐升高、作用时间逐渐延长，干物质和淀粉的降解率也逐渐降低。在所有处理组中，140℃35min处理组可以明显降低玉米的瘤胃淀粉降解率（P〈O．05），比未处理玉米组降低了15．26％。淀粉在瘤胃中降解的影响因素最主要的是淀粉的快速可溶部分和降解速率。     

Solid‐Surface Vitrification and In‐Straw Dilution After Warming of In Vitro‐Produced Bovine Embryos

Three experiments were designed to test a solid‐surface vitrification system for bovine in vitro‐produced embryos and to develop a simple method of in‐straw dilution after warming, which can be potentially used for direct transfer in the field. Experiment 1 evaluated embryo survival rates (i.e. re‐expansion and hatching) after vitrification and warming in three different solutions: VS1 (20% ethylene glycol (EG) + 20% propanediol (PROH) + 0.25 m trehalose (Tr)), VS2 (20% EG + 1M Tr) or VS3 (30% EG + 0.75 m Tr). Re‐expansion and hatching rates were higher (p < 0.05) for embryos vitrified in VS3 (72.2 ± 1.9 and 58.2 ± 0.8) than VS1 (64.4 ± 0.9 and 37.2 ± 2.5) or VS2 (68.5 ± 1.5 and 49.6 ± 1.0; p < 0.05). Experiment 2 was designed to compare two methods of vitrification: glass micropipettes or solid surface, using the VS1 or VS3 solutions. No significant differences were detected between the two methods; but re‐expansion and hatching rates were higher (p < 0.05) with VS3 (73.5 ± 3.1 and 47.1 ± 2.1) than VS1 (63.3 ± 3.3 and 39.7 ± 2.8). In experiment 3, embryos were vitrified by solid surface in VS1 or VS3 solutions and cryoprotectants were diluted in‐straw after warming in a TCM 199, 0.25 m sucrose solution or holding media. Survival rates of embryos vitrified in VS3 did not differ between those exposed to 0.25 m sucrose (74.7 ± 1.3 and 57.2 ± 2.2) or holding (77.3 ± 1.4 and 58.0 ± 2.5) medium after warming; however, survival rates of embryos vitrified in VS1 were higher (p < 0.05) in those exposed to 0.25 m sucrose (67.7 ± 2.3 and 47.0 ± 1.7) than holding medium (54.5 ± 1.0 and 27.7 ± 3.1). In conclusion, solid‐surface vitrification using simplified EG‐based solutions and in‐straw dilution with holding media may be a practical alternative for cryopreservation and direct transfer of in vitro‐produced bovine embryos.     

欧拉羊与山谷型藏羊杂交效果观察初报

引进草地型藏羊欧拉羊杂交改良山谷型藏羊,结果表明:引进的欧拉羊生理指标正常,对在林间草甸类和高山灌丛草场与山谷型藏羊同群放牧较适应;试验组受胎率、繁殖率、繁殖成活率比对照组分别高2.65%、2.69%、3.81%;欧×山杂交F1代初生公、母羊体高、体长、胸围及管围比同期对照组公、母羊分别提高了3.20 cm和4.50 cm、1.97 cm和2.25 cm、4.10 cm和2.20 cm、0.53cm和0.40 cm;体重提高0.62 kg和0.53 kg,比对照组分别提高了19.68个百分点和17.97个百分点。经过t检验,杂交F1代初生公、母羊体重、体高、体长、胸围极显著的高于对照组初生公、母羊(P0.01);管围差异显著(P0.05)。     

马尾松半同胞家系子代林早期生长差异分析

以湖北省京山县太子山林场管理局马尾松无性系嫁接种子园半同胞8年生子代林为研究对象,在对胸径、树高、枝下高、冠幅和材积等生长因子进行实地调查的基础上进行遗传力分析.结果表明:不同半同胞家系林木各生长因子之间存在极显著性差异,显示马尾松半同胞家系林木间存在较为丰富的遗传变异,具有较大的选育潜力;8年生马尾松半同胞家系子代林树高、胸径和材积的遗传力分别为0.7436、0.6055和0.6500;采用20%的入选率时,筛选出5个优良半同胞家系且入选半同胞家系的材积、树高和胸径遗传增益分别为51.34%、17.45%和22.19%.     

阿克苏引种面包小麦济南17号超高产栽培初探

阿克苏地区2000-2001年从山东省农科院引进强筋面包小麦新品种济南17号,在地区农业试验站进行品种区域适应性比较试验,并对其生育和植株性状进行了观测,在参试的8个品种中,济南17号平均产量6804kg/hm2,位居第三,较对照邯5316(地区小麦主栽品种)增产840kg/hm2,增产率14%。2001-2002年又在地区农业试验站对该品种示范种植800.04m2,产量8550kg/hm2,首次实现本地区引种面包小麦单产超8250kg/hm2的产量水平,两年试验示范结果表明,该品种在阿克苏地区具有较强的适应性和丰产性,同时取得了实现强筋小麦济南17号高产栽培的实践经验。     

Adaptation characteristics of mitochondria in plateau pikas at different altitudes

AIM: To investigate the adaptive characteristics of mitochondria in plateau pikas at different altitudes. METHODS: According to the altitudes of the capture area, plateau pikas were divided into 4 300 m group (Mado Star Sea, n=6) and 2 900 m group (Laoji Mountain South, n=6). The red blood cells and hemoglobin of the animals in 2 groups were measured. The levels of mitochondrial oxidative phosphorylation (OXPHOS) and fatty acid oxidation (FAO) in the liver and skeletal muscle, and mitochondrial H₂O₂ production level in the skeletal muscle were determined by high-resolution mitochondrial respirometer. RESULTS: The hemoglobin of the plateau pika in 4 300 m group was significantly lower than those in 2 900 m group. The liver state 4 respiration rate of mitochondrial respiratory chain complex I (CI-LEAK), mitochondrial respiratory chain complex I state 3 respiration rate (CI-OXPHOS) and respiratory chain complex I+Ⅱ state 3 respiration rate(CI+Ⅱ-OXPHOS), electron transfer capacity of respiratory chain complex I+Ⅱ (CI+Ⅱ-ETS), the electron transport capacity of the respiratory chain complex Ⅱ (CⅡ-ETS) in 2 900 m group were increased, and the coupling efficiency was decreased compared with 4 300 m group (P<0.05). The skeletal muscle CI-LEAK,CI+Ⅱ-OXPHOS and CI+Ⅱ-ETS were higher than those in 4 300 m group. Mitochondrial state 4 respiration rate with palmitic acid carnitine and malate as substrates (FAO-LEAK) of liver and skeletal muscle in 2 900 m group were increased compared with 4 300 m group. The skeletal muscle with palmitic acid carnitine as a substrate for fatty acid metabolism state 3 respiratory rate (FAO-OXPHOS) was increased in 2 900 m group compared with 4 300 m group. The H₂O₂/O₂ flow ratio (ΔH₂O₂) in 2 900 m group was lower in state 4 respiration rate of mitochondrial respiratory chain complex I (LEAK), state 3 of mitochondrial respiratory chain complex I (OXPHOS), state 3 of mitochondrial respiratory chain complex I+Ⅱ (OXPHOS*) and electron transfer capacity of respiratory chain complex I+Ⅱ (ETS) than 4 300 m group. CONCLUSION: Plateau pikas with different altitudes have different mitochondrial adaptation characteristics.     

不同温度挤压膨化玉米在泌乳奶牛瘤胃内干物质和淀粉降解规律的研究

本试验选用3头安装有永久性瘤胃瘘管的泌乳前期荷斯坦奶牛为试验动物,在精粗比为55∶45的日粮条件下,采用尼龙袋法测定了不同温度挤压膨化加工处理的玉米在奶牛瘤胃内干物质和淀粉的降解规律。试验结果表明,玉米经过挤压膨化加工处理,能明显提高干物质和淀粉在泌乳奶牛瘤胃中的降解率(P<0.05)。随着挤压膨化温度的不断升高,干物质和淀粉的瘤胃降解率线性下降。     

Efficacy of a novel copper-based footbath preparation for the treatment of ovine footrot during the spread period

Objective: To determine the efficacy of a novel copper based footbath preparation (CHF-1020) for treatment of ovine footrot during the spread period.
Design: A series of field trials with treated and control groups run together.
Animals: Mobs of at least 125 sheep on each of six properties in southern New South Wales with equal numbers of controls.
Procedure: Sheep of group A were treated after minimal paring by making them stand in CHF-1020 for 15 minutes. Treatment was undertaken at intervals throughout the period of the trials (14 September to 17 December 1993). Group A sheep were run on the same pasture as those from group B (untreated sheep).
Results: The percentage of sheep exhibiting clinical signs of ovine footrot at the start of the trial ranged from 35 to 88% at score 3 or higher, using a 0 to 5 footscoring system. During the trial, the percentage of infected sheep (greater or equal to score 2) in group B increased and ranged from 40 to 90%. The level of infected sheep in group A on each property was reduced progressively to 1 to 16%. Cure rates of 45 to 94% were achieved, with the lowest rate being on a property with a metal footbath. The next lowest cure rate was 73%. Results indicated that treatment should be undertaken at 2-weekly intervals while spread continues. Treated sheep can be returned to contaminated pastures.
Conclusion: CHF-1020 is effective during the spread period and can be used for the progressive eradication of ovine footrot.