Evaluation of platelet-rich plasma applied in the coronary band of healthy equine hooves

Platelet-rich plasma (PRP) is a widely used hemocomponent that holds great promise in equine medicine due to its feasible production and regenerative therapy potential. Its use has been considered as a treatment for chronic laminitis, mainly in terms of its analgesic properties and because it can induce growth in affected hooves. The aim of this study was to evaluate the effect on hoof growth attributable to PRP applied in the coronary band of clinically healthy horses by comparing the responses to PRP, saline, and trimming alone. After randomization, the forelimbs of 9 horses received specific treatment at intervals of 33 days and were trimmed, measured, and radiographed at the same time. Neither hoof growth nor hoof angles were different between the treatment groups at any time point. The application of PRP in the coronary band of horses may be a safe procedure; however, it had no effect on the growth or conformation of hooves in clinically healthy horses.     

BAR domains as sensors of membrane curvature: the amphiphysin BAR structure

The BAR (Bin/amphiphysin/Rvs) domain is the most conserved feature in amphiphysins from yeast to human and is also found in endophilins and nadrins. We solved the structure of the Drosophila amphiphysin BAR domain. It is a crescent-shaped dimer that binds preferentially to highly curved negatively charged membranes. With its N-terminal amphipathic helix and BAR domain (N-BAR), amphiphysin can drive membrane curvature in vitro and in vivo. The structure is similar to that of arfaptin2, which we find also binds and tubulates membranes. From this, we predict that BAR domains are in many protein families, including sorting nexins, centaurins, and oligophrenins. The universal and minimal BAR domain is a dimerization, membrane-binding, and curvature-sensing module.     

Effects of suboptimal low temperature on yield,fruit appearance and quality in muskmelon (Cucumis melo L.) cultivars

Summary

The effects of suboptimal low temperature were investigated on an array of yield, fruit appearance and fruit quality characters in 23 cultivars of melon, Cucumis melo L. The cultivars were grown in two temperature regimes: (I) a heated greenhouse (T1) with temperatures similar to those used in commercial cultivation in the winter in Israel; and (ii) an unheated greenhouse (T2) that had significantly lower night temperatures than T1. Significant differences were found among the cultivars in all characters. Plants from T2 showed an extended fruiting period, more fruits and higher total yield but smaller and lighter fruits than in T1. Fruits from T2 also had significantly more netting and higher amounts of total soluble sugars (TSS), sucrose and fructose than fruits from T1. Hybrid cultivars showed significantly higher electrical conductivity (EC), pH, acidity, TSS, sucrose, fructose and total sugars than open pollinated cultivars. Three trends were found among the characters in respect to the performance of the cultivars between T1 and T2: (a) most cultivars had significantly higher amounts in T2 than T1 (fruit/plant, yield/plant and fructose); (b) only cultivars with amounts below the pooled mean in T1 had higher amounts in T2 (percent dry weight, sucrose, TSS and total sugars); (c) no trend was found between T1 and T2 (mean fruit weight, pH, EC, glucose). Suboptimal low temperatures, when used appropriately, can aid in improving fruit quality.     

Whole-genome shotgun assembly and analysis of the genome of Fugu rubripes

The compact genome of Fugu rubripes has been sequenced to over 95% coverage, and more than 80% of the assembly is in multigene-sized scaffolds. In this 365-megabase vertebrate genome, repetitive DNA accounts for less than one-sixth of the sequence, and gene loci occupy about one-third of the genome. As with the human genome, gene loci are not evenly distributed, but are clustered into sparse and dense regions. Some "giant" genes were observed that had average coding sequence sizes but were spread over genomic lengths significantly larger than those of their human orthologs. Although three-quarters of predicted human proteins have a strong match to Fugu, approximately a quarter of the human proteins had highly diverged from or had no pufferfish homologs, highlighting the extent of protein evolution in the 450 million years since teleosts and mammals diverged. Conserved linkages between Fugu and human genes indicate the preservation of chromosomal segments from the common vertebrate ancestor, but with considerable scrambling of gene order.