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71.
Yamamoto Y Ishikawa R Omoe K Yoshikawa N Yamaguchi-Yamada M Taniguchi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(3):265-271
The inwardly rectifying K+ channels, Kir1.1, Kir2.3 and Kir4.1-Kir5.1, are the candidate chemosensory molecules for CO2/H+. We determined the mRNA expression and immunohistochemical localization of these channels in the medulla oblongata of the rat. RT-PCR analysis revealed mRNAs of Kir1.1, Kir2.3, Kir4.1 and Kir5.1 were detected in the medulla. The immunoreactivities for Kir1.1, Kir2.3, Kir4.1, and Kir5.1 were observed in the medulla, and immunolabeling pattern was varied by the subunit. Immunoreactivities for Kir1.1 and Kir2.3 were observed in the nerve cell bodies and glial cells both in the chemosensory areas [nucleus tractus solitarius (NTS), nucleus raphe obscurus (RO), pre-B?tzinger complex (PreB?tC)] and non-chemosensory area [hypoglossal nucleus (XII), inferior olive nucleus (IO)]. Kir4.1 immunoreactivity was observed in the glial cells and neuropil, especially in XII and IO. Kir5.1 immunoreactivity was observed in the nerve cell bodies in the XII, RO, and PreB?tC, but not in the NTS or IO. In the NTS, a dense network of varicose nerve fibers showed immunoreactivity for Kir5.1. Our findings suggest that Kir channels may not act specific to the central chemoreception, but regulate the ionic properties of cellular membranes in various neurons and glial cells. 相似文献
72.
73.
Kelly Vargas Yoshiho Asakura Minoru Ikeda Nobuhiko Taniguchi Yasuhiro Obata Katsuyuki Hamasaki Kotaro Tsuchiya Shuichi Kitada 《Fisheries Science》2008,74(3):533-543
ABSTRACT: Allozyme variation of the littleneck clam Ruditapes philippinarum was evaluated in four samples from Nameishi and Matsuo in the Ariake Sea, Ryugatake and Ushibuka in the Shiranui Sea off Kyushu Island, Japan, and in one sample from Jinzhou, China, in the Bohai Sea. A Ruditapes bruguieri sample imported from the Korean Bay off Nampo, North Korea was also studied. Among the R. philippinarum samples, heterozygosity varied from 0.265 to 0.301 and F IS estimates indicated significant homozygosity excess in 15 of 40 loci analyzed. Deviations from Hardy–Weinberg equilibrium were significant in all samples ( P < 0.05). Pairwise F ST estimates indicate that genetic differences between the Chinese and Japanese samples were very low, but significantly different from zero. Mixture proportions with 95% confidence intervals of Chinese R. philippinarum in Nameishi and Matsuo were estimated at 0.4098 [0.2512, 0.5705] and 0.4899 [0.3262, 0.6540], respectively. However, genetic invasion of stocked Chinese R. philippinarum into wild populations in the Ariake Sea remains uncertain due to the low precision of the estimates caused by the high similarity of allele frequencies between Jinzhou and the Ariake Sea. 相似文献
74.
M Hiruma K Ogawa K Taniguchi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(5):881-889
Effects of dehydration on vasopressin-secreting cells (VP cells) of the supraoptic nucleus (SON) and the paraventricular nucleus (PVN) in the Mongolian gerbils were studied immunocytochemically and morphometrically. The plasma osmolality was measured at the time of sacrifice of individual animals and the body weight was measured every day during dehydration. The plasma osmolality increased significantly on day 3 of dehydration, followed by a gradual increase to reach nearly its equibilium state on day 10. The body weight decreased rapidly until day 10, followed by a gradual decrease thereafter. The area of VP cells increased significantly in both the SON and PVN on day 1 of dehydration, the level being nearly the same until days 3 to 5 and going up on day 7 to reach the plateau after day 15. These findings seem to reflect a compensation mechanism between the volume of body fluid and the plasma osmolality and to reflect responses of VP cells to the osmotic stimuli. Electron microscopic observation revealed that, at the beginning and late stages of dehydration, the increase in the area of VP cells was in parallel with the expansion of the Golgi area and with the distension of cisternae of the rough endoplasmic reticulum. 相似文献
75.
Oikawa T Saito H Taniguchi K Taniguchi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(7):759-765
Differential maturation of three types of olfactory organs, the olfactory epithelium (OE), the vomeronasal organ (VNO) and the septal olfactory organ of Masera (MO), was examined immunohistochemically in embryonic and newborn rats by the use of antiprotein gene product 9.5 (PGP 9.5) serum. These olfactory organs were derived in common from the olfactory placode as neuroepithelia. In the OE, PGP 9.5-immunopositive olfactory cells first appeared at 13 days of gestation. The OE maturated completely, and showed the same cytological features as in the adult at 20 days of gestation. The MO first appeared as a dense mass of PGP 9.5-immunopositive sensory cells on the most ventrocaudal part of the nasal septum at 15 days of gestation and was evidently isolated from the OE by the decrease of immunopositive cells in the intercalated epithelium between the OE and the MO at 20 days of gestation. However, even at 7 days after birth, the MO did not complete its development and contained sensory cells aggregating in the mass. The VNO was separated from the nasal cavity at 13 days of gestation as a tubular structure of a neuroepithelium including PGP 9.5-immunopositive sensory cells. These cells gradually increased in number in the sensory epithelium of the VNO and extended their dendritic processes to the free surface at 7 days after birth. These findings clarified the differential maturation of these olfactory organs. That is, the OE completes its development before birth, while the MO and VNO after birth. 相似文献
76.
Taniguchi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1311-1314
The expression of T1R3, a taste receptor essential for the perception of sweetness and umami-taste, was examined by immunohistochemistry to determine whether and where it may be localized in the liver and pancreas. In the liver, both immunopositive and immunonegative reactions were detected; bile ducts and intercalated portions of the bile ductules were immunopositive to T1R3, while arterioles and venules were immunonegative in interlobular connective tissue. In the hepatic lobule, all other cells including liver cells (hepatocytes) and bile capillaries were immunonegative. In the pancreas, all endocrine portions of the pancreas were immunonegative to T1R3. Within the exocrine portions, immunopositive reactions were detected in excretory duct cells, intercalated cells, and centroacinar cells. In contrast, acinar cells were immunonegative, as were vessels, lymph capillaries, nerve fibers, and connective tissue cells in the exocrine portions. The restricted localization of T1R3 in the duct cells of the liver and pancreas in the present study may indicate that T1R3 is involved in monitoring changes in the makeup of bile and pancreatic juices in the hepatic and pancreatic duct systems. 相似文献
77.
Maeda M Yabuki A Matsumoto M Taniguchi K Suzuki S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(10):1259-1262
The kidneys of non-diabetic NOD and wild type ICR mice were examined morphometrically at 3 and 6 months of age. Kidney weights and diameter of renal corpuscles of non-diabetic NOD mice were less than those of ICR mice. No lesions were observed in glomeruli or uriniferous tubules. Renin-positive areas were more common in NOD mice than in ICR mice, but no differences were detected in the Western blot analyses. 相似文献
78.
Yamamoto Y Mori M Saito S Amano M Yamanome T Taniguchi K Oikawa T Yamamori K Taniguchi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1609-1611
Differentiation of the histochemical characteristics of the olfactory receptor cells (ORC) was examined by immunohistochemistry for protein gene product 9.5 (PGP 9.5) and calretinin (CR) and lectin histochemistry for Phaseolus vulgaris agglutinin-L (PHA-L) in the developing olfactory epithelium (OE) of the barfin flounder. PGP 9.5 immunoreactivity was diffuse and CR immunoreactivity was restricted at day 7, but these immunoreactivities became intense in the OE toward day 91. Crypt cells were first identified at day 56. PHA-L staining was faint at day 28, but became intense toward day 91. These findings suggest that PGP 9.5-immunopositive cells, CR-immunopositive cells, crypt cells and PHA-L-reactive cells differentiate independently in the developing OE and constitute subsets of the ORC in the OE. 相似文献
79.
Abanin DA Morozov SV Ponomarenko LA Gorbachev RV Mayorov AS Katsnelson MI Watanabe K Taniguchi T Novoselov KS Levitov LS Geim AK 《Science (New York, N.Y.)》2011,332(6027):328-330
Transport measurements have been a powerful tool for discovering electronic phenomena in graphene. We report nonlocal measurements performed in the Hall bar geometry with voltage probes far away from the classical path of charge flow. We observed a large nonlocal response near the Dirac point in fields as low as 0.1 tesla, which persisted up to room temperature. The nonlocality is consistent with the long-range flavor currents induced by the lifting of spin/valley degeneracy. The effect is expected to contribute strongly to all magnetotransport phenomena near the neutrality point. 相似文献
80.
K Yamasaki T Taga Y Hirata H Yawata Y Kawanishi B Seed T Taniguchi T Hirano T Kishimoto 《Science (New York, N.Y.)》1988,241(4867):825-828
Interleukin-6 (IL-6/BSF-2/IFN beta 2) is a multifunctional cytokine that regulates the growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. A complementary DNA encoding the human IL-6 receptor (IL-6-R) has now been isolated. The IL-6-R consists of 468 amino acids, including a signal peptide of approximately 19 amino acids and a domain of approximately 90 amino acids that is similar to a domain in the immunoglobulin (Ig) superfamily. The cytoplasmic domain of approximately 82 amino acids lacks a tyrosine/kinase domain, unlike other growth factor receptors. 相似文献