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101.
Dong-Jin Kang Hirofumi Tazoe Yasuyuki Ishii Katsunori Isobe Masao Higo Masatoshi Yamada 《Journal of Crop Science and Biotechnology》2018,21(2):113-119
Potassium (K) fertilizer closely affects both above-ground biomass production and radiocesium-137 (137Cs) uptake by plants from Cs-contaminated soil. Here we investigated the effects of low levels of K on aboveground biomass, 137Cs concentration, and 137Cs removal ratio (CR) using Napier grass (Pennisetum purpureum Schum.). Experimental plots fertilized with either 2 g K (20% K) or 6 g K (60% K) per m2 were compared with that with no K fertilization (0% K) under low-, medium-, and high-density planting conditions. As a result, with increasing amounts of K fertilizer, the aboveground biomass of Napier grass tended to increase, and the 137Cs concentration significantly decreased. The greatest CR was observed at the plot fertilized with 20% K and with plants grown under highly dense conditions. CR was positively correlated with biomass in all the experimental plots. Moreover, a positive correlation between CR and 137Cs concentration was found only at low levels of K application (0% and 20% K). In the present study, the application of 20% K produced more aboveground biomass and smaller decreases in 137Cs concentration in Napier grass compared with those with 0% K application; therefore, applying a fertilizer with low levels of K, such as 20% K, is better for 137Cs decontamination than applying 0% or 60% K fertilizer. 相似文献
102.
大麦过氧化物体膜上的抗坏血酸过氧化物酶拟南芥的耐盐机制 总被引:1,自引:0,他引:1
Ascorbate peroxidases (APX), localized in the cytosol, peroxisome, mitochondria, and chloroplasts of plant cells, catalyze the reduction of H2O2 to water by using ascorbic acid as the specific electron donor. To determine the role of peroxisomal type ascorbate peroxidase (pAPX), an antioxidant enzyme, in protection against salt-induced oxidative stress, transgenic Arabidopsis thaliana plant carrying a pAPX gene (HvAPX1) from barley (Hordeum vulgate L.) was analyzed. The transgenic line pAPX3 was found to be more tolerant to salt stress than the wild type. Irrespective of salt stress, there were no significant differences in Na^+, K^+, Ca^2+, and Mg^2+ contents and the ratio of K^+ to Na^+ between pAPX3 and the wild type. Clearly, the salt tolerance in pAPX3 was not due to the maintenance and reestablishment of cellular ion homeostasis. However, the degree of H2O2 and lipid peroxidation (measured as the levels of malondialdehyde) accumulation under salt stress was higher in the wild type than in pAPX3. The mechanism of salt tolerance in transgenic pAPX3 can thus be explained by reduction of oxidative stress injury. Under all conditions tested, activities of superoxide, glutathione reductase, and catalase were not significantly different between pAPX3 and the wild type. In contrast, the activity of APX was significantly higher in the transgenic plant than in wild type under salt stress. These results suggested that in higher plants, HvAPX1 played an important role in salt tolerance and was a candidate gene for developing salttolerant crop plants. 相似文献
103.
104.
Toriumi H Tsumagari S Kuwahara Y Ichikawa Y Takeishi M Sakai T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(2):243-247
In ultrasonographic diagnosis of ovarian disorders and the estrous cycle in sows, transverse observation of the uterus yielded more characteristic findings than observation of sagittal sections. Transverse ultrasonography revealed that the low progesterone (P) type uterus showed a round structure, while the high P type uterus showed a flattened structure. These results corresponded well with rectal palpation findings: the low P type uterus had a hard, pipe-like structure and the high P type a soft, balloon-like structure. For gilts, we employed a minimum convex type transrectal prostate probe that had an approximately 18 cm insertion handle. The images of the uterus obtained thereby were a similar to those obtained from sows. The above results suggest that it should be possible to diagnose and treat many ovarian disorders in sows and gilts based only on the ultrasonograhic findings. In short, ultrasonograhic findings of a round structure of the uterine wall might be an indication for PMSG (pregnant mare serum gonadotropin) treatment, while findings of a flattened structure might be an indication for PGF(2) (alpha) administration. 相似文献
105.
Molecular cloning of canine monoamine oxidase subtypes A (MAOA) and B (MAOB) cDNAs and their expression in the brain 总被引:4,自引:0,他引:4
Hashizume C Suzuki M Masuda K Momozawa Y Kikusui T Takeuchi Y Mori Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(8):893-898
The role of monoamine oxidase has been shown to be related to some behavioral changes including aggression and cognitive dysfunction. In order to demonstrate the basic expression patterns of monoamine oxidase in the canine brain, we determined the full-length nucleotide sequences of cDNA for canine monoamine oxidase type A (MAOA) and type B (MAOB) genes that were isolated from the canine brain cDNA library. Oligonucleotide primers for PCR were constructed based on the conserved sequences reported thus far for other mammalian species. The nucleotide sequences had open reading frames of 1584 and 1563 bp for MAOA and MAOB, respectively. Both of these genes showed relatively high homology with other species in both nucleotide (> 81%) and deduced amino acid (> 85%) sequences. In Northern blot analyses MAOA mRNA was expressed broadly in various parts of the canine brain, whereas MAOB mRNA was found only in specific brain regions, such as the hypothalamus, hippocampus, brain stem and olfactory bulb. These results suggest that MAOA and MAOB mRNAs have subtype-specific expression patterns in the canine brain. 相似文献
106.
To examine whether the pathogenic bacterium, Erwinia carotovora subsp. carotovora, causal agent of soft rot of Chinese cabbage (Brassica campestris L., pekinensis group), can overwinter in plant debris and soil and serve as inoculum the following year, we monitored field
populations of rifampicin-resistant, phage-sensitive strains of the bacterium. Chinese cabbage (cv. Matsushima Kohai W1116)
were planted in field soil in pots that were sunk into the field on Aug. 2, 1996 and eventually reduced to one plant per pot.
Outer petioles of the plants were inoculated with mixture of 13 bacterial strains of E. carotovora subsp. carotovora on Sept.5, 1996. After the soft rot spread throughout the plant, the diseased plant was buried in the potted soil. New seeds
were sown in the pots on April 30, 1997, and the disease was observed in June and July. The bacterial strains were re-isolated
from the potted soil, diseased tissue and rhizosphere soil by the dilution plating method on modified Drigalski's medium containing
100 ppm rifampicin and by the enrichment technique. In addition to rifampicin resistance, phage sensitivities of some of the
re-isolated strains were identical to those of the strains buried in the soil with the diseased plant in the previous year.
From these results, some of the 13 strains overwintered in the soil and infested plant tissue and acted as primary inoculum
the following year. The frequency of re-isolation varied among the strains, perhaps because of competition among the strains,
differences in epidemiological behavior and stabilizing selection among the strains, and the presence of different ecotypes
of the organism.
Received 21 July 2000/ Accepted in revised form 19 September 2000 相似文献
107.
Hideki YAMAMOTO Tadahiko KIGUCHI Mineo SENDA Kenji UEDA Masayasu INOUE Tsutomu MATSUMOTO 《Journal of General Plant Pathology》2001,67(4):309-311
The three missense mutations on the gene for the 130-K protein of Tomato mosaic virus (ToMV) L11A have been thought to be responsible for the attenuation of its virulence. The Eco47I RFLP detecting the missense mutation at 2349 successfully discriminated L11A and its derivative attenuated isolates from ToMV virulent ones. RFLP analysis and mismatch amplification assay detecting
the missense mutations at 1117 and 2754, respectively, could not discriminate some of the attenuated isolates from the virulent
ones. These results indicated that, of the three missense mutations, only the one at 2349 was conserved in all the L11A-derivative attenuated isolates.
Received 16 March 2001/ Accepted in revised form 22 June 2001 相似文献
108.
Otani I Niwa T Tajima M Ishikawa A Watanabe T Tsumagari S Takeishi M Kanayama K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(5):441-444
CD56+ cells in canine blood leukocytes were characterized by flow-cytometric analysis of peripheral blood of 30 healthy adult beagle-dogs (15 males and 15 non-pregnant females). In 19 of the 30 dogs, anti human CD56 antibody, Leu-19, reacted with 8.8-21.7% of peripheral blood lymphocytes. All CD56+ cells simultaneously expressed CD3 molecules on their surface. Further phenotypic analysis revealed that 50.6+/-13.1% of the CD56+ cells showed CD4-CD8+ phenotype and 43.7+/-10.1% showed CD4+CD8- phenotype. Expression intensity of CD56 on the CD4-CD8+CD56+ cells was significantly higher than that on CD4+CD8-CD56+ cells (P<0.001). These findings indicate that CD56, which is a neural cell adhesion molecule, is uniquely expressed on subsets of T lymphocytes in canine peripheral blood. 相似文献
109.
110.
Chikara MASUTA Yuko SESHIMO Motomi MUKOHARA Hye Jin JUNG Shigenori UEDA Ki Hyun RYU Jang Kyung CHOI 《Journal of General Plant Pathology》2002,68(2):163-168
We analyzed the evolutionary histories of two lily strains of Cucumber mosaic virus (CMV) isolated in Japan and Korea (HL- and Ly2-CMVs). They share common biological characteristics in that their host ranges
are very restricted perhaps from a unique adaptation to lily plants. Although HL and Ly2 were isolated independently from
different lily species in separate countries, their RNA3 sequences had a very high sequence similarity (97%). The evolutionary
relationships between the two isolates were characterized by comparing their phylogenetic trees for the 3a and CP genes. The
two lily CMVs always formed a distinct cluster within subgroup IB in 3a, but within IA in CP. Together, the phylogenetic tree
topology and the sequence identity between the two lily CMVs suggest that they evolved from a common progenitor.
Received 5 November 2001/ Accepted in revised form 11 January 2002 相似文献