Characterization of wood-based molding bonded with citric acid

The wood-based moldings were fabricated by using only citric acid as an adhesive. The mechanical properties, water resistances, thermal properties and chemical structure were investigated. Wood powder obtained from Acacia mangium was mixed with citric acid under certain weight ratios (0–40 wt%), and each powder mixture was molded using two types of metal molds at 200°C and 4 MPa for 10 min. The modulus of rupture and the modulus of elasticity values of the wood-based molding containing 20 wt% citric acid were 35.8 MPa and 5.4 GPa, respectively. The maximum impact strength was 0.94 kJ/m² with the same citric acid content. The water resistance increased with increasing citric acid content, and the good resistance to boiling water was also recognized. However, some elution of substances derived from citric acid was observed at high levels of citric acid, causing a decrease in the thermal properties. The ester linkages were detected by Fourier transform infrared spectroscopy, indicating that the citric acid reacted with the wood. Our results demonstrated that citric acid brought about good adhesiveness for fabricating the wood-based molding.     

Alternative BSE risk assessment methodology for beef and beef offal imported into Japan

The Food Safety Commission (FSC) of Japan, established in July 2003, has its own initiative to conduct risk assessments on food stuffs known as "self-tasking assessment". Within this framework, the FSC decided to conduct a risk assessment of beef and beef offal imported into Japan from countries with no previous BSE reports; thus, a methodology was formed to suit to this purpose. This methodology was partly based on the previous assessments of Japanese domestic beef and beef imported from U.S.A./Canada, but some modifications were made. Other organizations' assessment methods, such as those used for BSE status assessment in live cattle by the OIE and EFSA's GBR, were also consulted. In this review, the authors introduce this alternative methodology, which reflects (1) the risk of live cattle in the assessed country including temporal risks of BSE invasion and domestic propagation, with the assessment results verified by surveillance data, and (2) the risk of beef and beef offal consisting of cumulative BSE risk by types of slaughtering and meat production processes implemented and the status of mechanically recovered meat production. Other possible influencing factors such as atypical BSE cases were also reviewed. The key characteristic of the current assessment is a combination of the time-sequential risk level of live cattle and qualitative risk level of meat production at present in an assessed country.     

The effects of inclusion levels of urea-treated potato pulp silage in concentrate and roughage sources on finishing performance and carcass quality in cull beef cows

An experiment was conducted to determine the effects of inclusion levels of potato pulp silage in concentrate (0, 15, 30 or 45%; on a dry matter basis) and roughage sources (rice straw or wheat straw) on finishing performance and carcass quality of cull beef cows. Sixteen Japanese Black (Wagyu) mature cull cows (490 ± 31 kg of BW) were used in this experiment. Increasing the levels of potato pulp silage in concentrate and roughage sources did not significantly affect feed intake in cows. In addition, the final body weight, daily gain and feed : gain ratio were not influenced by the inclusion levels of potato pulp silage in concentrate and the type of roughage. Increasing the inclusion levels of potato pulp silage in concentrate would probably decrease the marbling score. The L* values of the longissimus muscle (LM) tended to respond quadratically ( P  = 0.078) as the inclusion levels of potato pulp silage in concentrate increased, and was lowest for cows fed the concentrate which included 30% potato pulp silage. The a* and b* values of the LM and fat color were not affected by the inclusion levels of potato pulp silage in concentrate. No effects of roughage sources on finishing performance were observed.     

Manufacture and properties of high-performance oriented strand board composite using thin strands

Three-layered composite oriented strand boards were manufactured using very thin hinoki (Japanese cypress, Chamaecyparis obtusa Endl.) strands oriented in the faces and mixtures of sugi (Japanese cedar, Cryptomeria japonica D. Don.) and hinoki particles in the core. The boards were composed of two density levels, with 1:8:1, 0.5: 9 : 0.5, and 0: 10 : 0 face: core: face ratios. Polymeric and emulsion type isocyanate resins were used. The resin contents for the strands in the face and particles in the core were 10% and 5%, respectively. The steam-injection press was applied at 0.62MPa (160°C), and the steam-injection time was 2min. The mechanical and physical properties of the boards were evaluated based on the Japanese Industrial Standard. The parallel moduli of rupture and elasticity along the strand orientation direction and the wood screw retaining force increased with increasing face/core ratios. Incorporation of 10%–20% of thin strands in the face of the boards improved the parallel moduli of rupture and elasticity by 47%–124% and 30%–65%, respectively. In addition, the thickness swelling after water-soaking at 20°C for 24h, and the parallel linear expansion after boiling for 2h and water-soaking at 20°C for 1 h, of the three-layered composite boards were below 8% and 0.15%, respectively, despite a short steam-injection press time. The thickness swelling of the boards decreased with increasing face/core ratios. In contrast, the presence of face strands seems to have a minimal effect on the moduli of rupture and elasticity along the perpendicular direction of the three-layered composite boards. A similar trend was observed for the internal bond strength, hardness, and linear expansion along the perpendicular direction.This paper was presented at the 47th annual meeting of the Japan Wood Research Society, Kochi, April 1997     

Effects of mat moisture content and press closing speed on the formation of density profile and properties of particleboard

Isocyanate resin-bonded 0.5 and 0.7 g/cm³ lauan (Shorea sp.) particleboards were produced from mats with uniform and distributed moisture content (MC) distributions, using three hot press closing speeds. The effects of these processing variables on the formation of density profile in particleboard and board properties were analyzed statistically. A definition of the density profile was introduced, and the correlations among the various defining factors were established. The results are summarized as follows. (1) The peak density (PD) of particleboard could be increased, with a slight reduction in the core density (CD), using mats with different MC distributions. (2) In a conventional density profile, CD and PD are highly dependent on the board mean density (MD); and the gradient factor (GF), peak distance from the faces (Pdi), and peak base (Pb) are significantly correlated to each other, at the 99% significance level. (3) Greater press closing speed reduces Pdi and Pb, with an increase in GF. (4) Greater press closing speed could increase the PD in board of low MD, with minimal effect on CD. (5) The modulus of elasticity (MOE) of particleboards from mats with high MC near the faces were consistently higher than those from mats with uniform MC, irrespective of the press closing speed, whereas their modulus of rupture (MOR) became indifferent at higher MD under slow and fast closing speeds. (6) Sanding does not improve the MOR and MOE of particleboard significantly.     

Estimating the spawning season of black sea bream Acanthopagrus schlegelii in Hiroshima Bay,Japan, from temporal variation in egg density

Fisheries Science - We aimed to determine the spawning period of black sea bream Acanthopagrus schlegelii in Hiroshima Bay, Japan, based on the distribution of its eggs, to assist in the resource...     

Antiradical function of sulfhydryl residues and oxidative stress markers in dairy cattle plasma

The antiradical function of sulfhydryl (SH) residue in dairy cattle plasma and the relationship of SH residue concentrations to other oxidative stress markers, ascorbic acid and thiobarbituric acid reactive substance (TBARS) in plasma were investigated. The concentration of reduced glutathione (GSH) in phosphate buffer solution (pH 7.6) and SH residues in dairy cattle plasma decreased significantly (P < 0.05) in vitro by the addition of peroxyradicals at 38°C, depending on incubation periods. The decrease of GSH concentration with the peroxyradical solution was partially protected by the addition of sodium ascorbate solution. A positive and significant correlation with SH residues and albumin concentration in the fresh plasma obtained from 15 dairy cattle was observed (P < 0.05). The SH residue concentration was not correlated with the TBARS concentration in plasma. The total ascorbic acid and SH residues concentration in the plasma correlated positively but not significantly (P < 0.10). These results suggested that SH residues in dairy cattle plasma play important part in the antiradical function.     

Anisotropic thermal properties of molded carbon phenolic spheres

Anisotropic thermal properties of molded carbon phenolic spheres (CPS), a mixture of sugi wood charcoal powders and phenol formaldehyde resin molded with a hot press, were investigated. The effects of the carbonizing temperature, particle size of chars, and density of the CPS on thermal properties were discussed. The molded CPS specimens were measured for their thermal properties using the laser flash method in both horizontal and vertical directions. The configuration of the CPS was observed by scanning electron microscopy. Anisotropy of the thermal properties (thermal diffusivity and thermal conductivity) between horizontal and vertical directions of the molded CPS was much higher than that of the uncarbonized molded phenolic spheres. Therefore, converting wood into molded CPS is an effective way to enhance the thermal-anisotropy properties. More marked effects of the carbonizing temperature, particle size, and density were observed in the horizontal direction than in the vertical direction. Anisotropy in thermal properties of the molded CPS may be considered an advantage for developing a new fire-retardant material for wood composites.An outline of this study was presented at the 47^th and 48^th annual meetings of the Japan Wood Research Society, Kochi and Shizuoka, 1997 and 1998.     

Transgenic Pigs with Pancreas-specific Expression of Green Fluorescent Protein

The development and regeneration of the pancreas is of considerable interest because of the role of these processes in pancreatic diseases, such as diabetes. Here, we sought to develop a large animal model in which the pancreatic cell lineage could be tracked. The pancreatic and duodenal homeobox-1 (Pdx1) gene promoter was conjugated to Venus, a green fluorescent protein, and introduced into 370 in vitro-matured porcine oocytes by intracytoplasmic sperm injection-mediated gene transfer. These oocytes were transferred into four recipient gilts, all of which became pregnant. Three gilts were sacrificed at 47–65 days of gestation, and the fourth was allowed to farrow. Seven of 16 fetuses obtained were transgenic (Tg) and exhibited pancreas-specific green fluorescence. The fourth recipient gilt produced a litter of six piglets, two of which were Tg. The founder Tg offspring matured normally and produced healthy first-generation (G1) progeny. A postweaning autopsy of four 27-day-old G1 Tg piglets confirmed the pancreas-specific Venus expression. Immunostaining of the pancreatic tissue indicated the transgene was expressed in β-cells. Pancreatic islets from Tg pigs were transplanted under the renal capsules of NOD/SCID mice and expressed fluorescence up to one month after transplantation. Tg G1 pigs developed normally and had blood glucose levels within the normal range. Insulin levels before and after sexual maturity were within normal ranges, as were other blood biochemistry parameters, indicating that pancreatic function was normal. We conclude that Pdx1-Venus Tg pigs represent a large animal model suitable for research on pancreatic development/regeneration and diabetes.