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81.
Sakurako NEO Iyo TAKEMURA-UCHIYAMA Jumpei UCHIYAMA Hironobu MURAKAMI Ayaka SHIMA Hideki KAYANUMA Taiki YOKOYAMA Satoshi TAKAGI Eiichi KANAI Masaharu HISASUE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(7):1019
Although the biliary system is generally aseptic, gallbladder microbiota has been reported in humans and some animals apart from dogs. We screened and analyzed the bacterial deoxyribonucleic acid in canine gallbladders using bile sampled from 7 healthy dogs and 52 dogs with liver- or gallbladder-associated disease. PCR screening detected bacteria in 17.3% of diseased dogs (9/52) and none in healthy dogs. Microbiota analysis of PCR-positive samples showed that the microbial diversity differed between liver- and gallbladder-associated disease groups. Thus, a specific bacterial community appears to occur at a certain frequency in the bile of diseased dogs. 相似文献
82.
Kawarai S Hashizaki K Kitao S Nagano S Madarame H Neo S Ishikawa T Furuichi M Hisasue M Tsuchiya R Tsujimoto H Yamada T 《Veterinary immunology and immunopathology》2006,113(1-2):30-36
A primary cultured cell line named CHKS was established from a hepatocellular carcinoma (HCC) of a dog showing a high level of serum alpha-fetoprotein (AFP). CHKS secreted a 66 KDD AFP into the growth medium regardless of the presence or absence of fetal bovine serum (FBS). Cloning CHKS with limiting dilution produced 4 clones, CHKS-1, -2, -3, and -4, which secreted 826, 471, 70, and less than 10 ng/ml, respectively, of AFP into the culture medium. In culture, these cell lines were similar in morphology and proliferation pattern to epithelial cells and positive to periodic acid-Schiff (PAS) staining. The presence of mRNA for canine albumin was demonstrated by nested PCR. The doubling times of the clone cell lines were 21, 45, 36, and 35 h, saturation densities 34, 18, 22, and 24 x 10(4)/cm(2), and plating efficiencies 18, 45, 46, and 45%, respectively. Chromosome analysis of these cell lines showed near triploidy. These results show that CHKS and its clones have hepatic cell functions and are useful for carcinogenetic and clinical studies of canine HCC. 相似文献
83.
Hiroshi Saotome Hideaki Korai Takahiko Iida Tomoko Hamano Kensuke Kawarada Masaharu Ohmi 《Journal of Wood Science》2012,58(5):423-428
An air-injection press (AIP) was developed to prevent accidental blowouts of boards during production. In this study, the effects of the AIP on preventing blowouts were investigated by artificially creating a blowout-prone condition, and the press was shown to be effective in preventing blowouts. The modulus of rupture of the boards was almost constant irrespective of pressing time. Longer pressing time resulted in higher internal bond strength when pressed at 170 °C. The thickness swelling of the boards pressed at 170 or 190 °C was almost uniform irrespective of pressing time, and the manufactured boards showed performance similar to those manufactured with an ordinary press. The AIP prevented blowouts sufficiently even when the pressure of the injected air was reduced, and this reduction did not adversely decrease the performance of the boards. Air injection reduced formaldehyde emissions from the board. 相似文献
84.
Tadatoshi OHTAKI Masaharu MORIYOSHI Ken NAKADA Toshihiko NAKAO Yutaka SAWAMUKAI 《Animal Science Journal》2012,83(9):644-649
Blood estrone sulfate (E1S), estrone (E1), estradiol (E2) and progesterone (P4) in newborn piglets were measured to clarify the relationships among birth and placental weight, vitality of offspring and litter size. First, the association between vital status (normal, weak and stillborn) from 165 newborn piglets of 18 litters and steroid concentrations; second, steroid concentrations from 152 newborn normal piglets and litter size; and third, steroid content in fetal placenta from 50 newborn normal piglets of six litters and litter size, were investigated. In the normal group, the birth and placental weight were significantly higher than those in the other groups. Blood E1S levels in the stillborn group were significantly lower, whereas E1, E2 and P4 were significantly higher compared to the normal group. Blood and placental E1S levels in the small litter group were significantly higher than those in the other groups. However, there was no significant difference among the three litter size groups in the levels of steroid hormones in maternal blood. These results indicate that vitality of newborn piglets is related to E1S concentration of neonate, to birth weight and placental weight. However, steroid hormone concentrations of newborn piglets were greatly affected by the number of littermates. 相似文献
85.
Hiroshi Saotome Masaharu Ohmi Hiroshi Tominaga Kiyoharu Fukuda Yutaka Kataoka Makoto Kiguchi Yasushi Hiramatsu Atsushi Miyatake 《Journal of Wood Science》2009,55(3):190-196
High-density and high-resin-content boards were produced by phenolic resin impregnation into board materials prepared by the
water-vapor-explosion process (WVE) to develop high-durability wood composite boards for exterior use. Wet-dry cyclic tests
and accelerated weathering tests were conducted, and the fundamental properties were determined to examine the effect of resin
impregnation on board qualities. Bending and internal bond strength of resin-impregnated boards (I-board) satisfied the criterion
for 18-type particleboard described in JIS A 5908. Thickness swelling (TS) after 24-h water immersion was approximately 2%.
Resin impregnation improved the dimensional stability of the boards. In wet—dry cyclic testing, TS of I-board was the same
as that of plywood. The retention ratio of modulus of rupture of I-board was large; thus, I-board had high bond durability.
Color change of I-board was less than that of ordinary particleboard after a 500-h accelerated weathering test. I-Board had
lower surface roughness than boards produced by a spray application method (S-board) and higher water repellency, although
the difference in resin contents of the face layer was small. Thus, it is suggested that the surface properties and weatherability
of I-board were improved by impregnation of phenolic resin. High-density and resin-impregnated boards made from the WVE elements
are expected to withstand actual exterior use.
Part of this report was presented at the 54th Annual Meeting of the Japan Wood Research Society, Sapporo, August 2004 相似文献
86.
Kobayashi J Mori Y Okamoto K Akiyama R Ueno M Kitamori T Kobayashi S 《Science (New York, N.Y.)》2004,304(5675):1305-1308
We have developed an efficient system for triphase reactions using a microchannel reactor. Using this system, we conducted hydrogenation reactions that proceeded smoothly to afford the desired products quantitatively within 2 minutes for a variety of substrates. The system could also be applied to deprotection reactions. We could achieve an effective interaction between hydrogen, substrates, and a palladium catalyst using extremely large interfacial areas and the short path required for molecular diffusion in the very narrow channel space. This concept could be extended to other multiphase reactions that use gas-phase reagents such as oxygen and carbon dioxide. 相似文献
87.
Kenji?TakiiEmail author Hidetsuyo?Hosokawa Sadao?Shimeno Masaharu?Ukawa Akifumi?Kotani Yojiro?Yamada 《Fisheries Science》2005,71(3):499-503
ABSTRACT: The present study aimed to investigate a suitable anesthesia protocol for nutritional studies, fasting tolerance, and nutrient requirements for maintenance in juvenile northern bluefin tuna Thunnus orientalis weighing 0.8–0.9 g. The fish grew in a mass seedling production under completely controlled aquaculture. The solution of 0.2 mL 2-phenoxyethanol/L prepared by filtered seawater has been notably estimated as a anesthesia protocol for the juvenile, due to a shorter mean time necessary for anesthesia and recovery and higher survival rate at 24 h after recovery. All other anesthetics, 0.1 and 0.3 mL 2-phenoxyethanol/L, 0.05, 0.1 and 0.2 mL 4-allyl-2-methoxyphenol/L, and cool water temperature shocks at 16.8, 12.6 and 10.4°C, were inferior. In a 5-day tolerance test, the juvenile was fed sand lance Ammodytes personatus through the rearing period or fasted for 2, 3, 4 and 5 consecutive days. Survival rates were about 80 or 60, 50, 10 and 0%, respectively. From the relationships between days fasting and energetic and nutritional retentions, juvenile tuna require about 142.7 kJ, 5.46 g protein and 0.44 g fat/kg body weight a day for maintenance, indicating remarkably higher requirements than other aquaculture fish. 相似文献
88.
Poor reproductive efficiency is a worldwide problem that has affected the dairy industry
during the last several decades. In an attempt to explain the changes in reproductive
physiology caused by high milk production, a model of elevated steroid metabolism in
lactating dairy cows has been proposed. A slow increase in levels and low peak levels of
estradiol (E2) and progesterone (P4) characterize endocrine changes
in high producing cows. Similar changes have been reported in the repeat breeder cows. The
abnormal changes in E2 and P4 concentrations of these cows may cause
an improper uterine environment due to disturbed expression of growth factors and
cytokines in the endometrium. This review focuses on the alteration in epidermal growth
factor (EGF) profile in the endometrium during the estrous cycle. The normal cow has two
peaks of EGF concentrations on days 2–4 and 13–14. Low concentrations of EGF on these days
distinguished both high-producing and repeat breeder cows from normal cows. Alteration of
the EGF profile could be found in 70 and 40% of the repeat breeder and high-producing
cows, respectively. Treatment with a high dose of estradiol benzoate and an intravaginal
progesterone-releasing device restored the normal EGF profile in about 70% of the affected
cows. The cows having a normal EGF profile after treatment showed a higher pregnancy rate
than the cows with the altered profile. Further studies to understand the etiology of the
alteration in the EGF profile are needed to develop another treatment option and
preventive management for this problem. 相似文献
89.
Molecular survey of Mycoplasma haemofelis and 'Candidatus mycoplasma haemominutum' infection in cats in Yamaguchi and surrounding areas 总被引:1,自引:0,他引:1
Inokuma H Taroura S Okuda M Hisasue M Itamoto K Une S Nakaichi M Taura Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(8):1017-1020
A molecular survey of hemoplasma (Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum') in Yamaguchi Prefecture and surrounding areas was performed by using molecular methods. PCR-RFLP with HindIII revealed that 2 cats were infected with M. haemofelis, and 16 with 'C. Mycoplasma haemominutum' among 102 randomly selected cats. Partial 16S rRNA gene sequences of M. haemofelis and 'C. Mycoplasma haemominutum' determined in this study showed percent similarities of 98.3-99.8% and 96.4-100%, respectively, with those from other countries. Hemoplasma infections were more frequently detected in free-roaming cats than inside cats. Also, the status of FeLV infection was another significant risk factor for hemoplasma infection. 相似文献
90.
Komeno M Akimoto A Fujita T Aramaki T Aoki M Shimada T Ohashi F 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(1):53-57
To clarify the role of nitric oxide (NO) in hemodialysis (HD)-related hypotension, the relationship between plasma NO metabolites (NOx) and blood pressure changes, and the effect of N(G)-monomethyl-L-arginine (L-NMMA), a NO synthase inhibitor, on changes in blood pressure were evaluated in an experimental renal dysfunctional dog model. In order to create a renal dysfunction model, gentamicin was administered to male beagles in which 7 of 8 renal artery branches had been ligated. Normal renal functional and dysfunctional dogs underwent 3 hr of HD per day for 3 days. HD induced a transient decrease in mean blood pressure in the normal renal functional dogs. In renal dysfunctional dogs, a continuous hypotension occurred with a gradual increase in the plasma NOx concentration during HD. Although L-NMMA prevented the fall in blood pressure, it did not significantly change the plasma NOx concentration during HD. These results suggest that NO contributes to HD-related hypotension in renal dysfunctional dogs but the plasma NOx concentration does not reflect the change in blood pressure. 相似文献