The frequency of eight common point mutations in CYP21 gene in Iranian patients with congenital adrenal hyperplasia

BACKGROUND: Congenital Adrenal Hyperplasia (CAH, the inherited inability to synthesize cortisol) is one of the most common (1 in 10000 to 1 in 15000) autosomal recessive disorders. More than 95% of cases of CAH are caused by 21-hydroxylase deficiency (21-OHD). Females with severe, classic 21-OHD are exposed to excess androgens prenatally and are born with virilized external genitalia. Most patients cannot synthesize sufficient aldosterone to maintain sodium balance and may develop potentially fatal salt wasting crisis if not treated. METHODS: We applied allele specific PCR to detect the eight common mutations in the CYP21 gene in patients. Fifty unrelated patients with symptoms of classical CAH were studied. RESULTS AND CONCLUSION: Seventy percent of our subjects had these mutations. The most frequent mutations were found to be I2G and del-8 bp (28% and 13%, respectively). The frequencies of other alleles were as following: I172N, 9%; V281L, 3%; exon 6 cluster (I236N, V237E and M239K), 4%; Q318X, 9%; R356W, 5%; and P30L, 0%. The frequency of mutations did not differ substantially from other ethnics, however, a higher rate of del-8 bp (13%) was found in our population. The aim of this study was to detect common mutations for setting up a molecular method for prenatal diagnosis.     

Effect of Metasystox-R on marine Nitrosomonas sp. as a nitrification inhibitor

Metasystox-R is a systemic soluble liquid insecticide for the control of aphids on brassica vegetable crops, cotton and lupins and it is possible enter to the marine environment and may be have a hazard effects for the marine organisms and nitrification processes. Effect of Metasystox-R on ammonia oxidizing activity by marine Nitrosomonas sp. was investigated by determining nitrification inhibitor assay in the cell suspension. Results showed that 8 microg mL(-1) of Metasystox-R with PI50 = 4.48 significantly inhibited nitrite production by marine Nitrosomonas sp. These results suggested marine Nitrosomonas sp. may be one of the target bacteria which was inhibitor and decreasing nitrification in the marine environment.     

Is there any association between overweight, obesity and symptoms of reflux disease?

The present study was aimed to identify the association of overweight and obesity with gastroesophageal reflux disease (GERD). This age- and sex-matched case-control study was carried out in a sample of subjects referred to the specialized clinic of Tabriz University of Medical Sciences from November 2006 to March 2007. Data were collected using a demographic questionnaire and a checklist to determine reflux symptoms. Weight and height were measured and Body Mass Index (BMI) was calculated. Logistic regression was used to examine the association between overweight, obesity and reflux symptoms. The study population included 106 cases (with reflux symptoms) and 111 controls with a mean age of 35.2 +/- 12.9 years. The mean BMI was 2.4 units greater in cases than controls (p = 0.0001). In unadjusted model, overweight (OR = 3.41, CI: 1.8-6.44) and obesity (OR = 2.84, CI: 1.38-5.82) were significantly associated with GERD. Results of multivariate logistic regression revealed significant association between overweight and GERD after adjusting for confounders (OR = 2.87, CI: 1.49-5.53). Given the increasing prevalence of patients with both overweight/obesity and GERD, Serious dietary intervention studies for weight loss as a therapeutic strategy should be carried out in GERD patients complicated with obesity.     

A retrospective survey of hydatidosis in livestock in Shiraz, Iran, based on abattoir data during 1999-2004

Meat inspection records in an abattoir located in Shiraz, a city in southwest Iran and the capital of Fars province, during a 5-year period from 20 March 1999 to 19 March 2004 were used to determine the prevalence of hydatidosis in sheep, cattle, and goats in the region. A total of 844,039 animals (cattle 131,716; sheep 577,090; goats 135,233) slaughtered in the 5-year period and overall 34,856 (4.1%) livers and 123,402 (14.62%) lungs were condemned. Hydatidosis was responsible for 28.7 and 15.4% of total livers and lungs condemnations, respectively. The overall trend for all livestock and in both organs was a significant downward one. The annual prevalence of liver condemnations due to hydatidosis was decreased from 2.1, 1.8, and 1.0% in 1999-2000 to 0.62, 0.33, and 0.20% in 2003-2004 for cattle, sheep, and goats, respectively. The corresponding features for lung condemnation due to hydatidosis were relatively higher than liver, declining from 3.0, 4.6, and 4.4% in 1999-2000 to 1.3, 1.5, and 0.56% in 2003-2004, for cattle, sheep, and goats, respectively. Greater awareness among farmers, destruction of organs containing hydatid cysts, prevention of access of dogs to raw offals and implementation of national rabies control program could be responsible factors. Data showed significant seasonal pattern for hydatidosis in all species. Liver condemnations due to hydatidosis were higher in the spring and summer for sheep, whereas lung condemnations were higher in summer for both sheep and goats. Liver and lung condemnations in cattle were higher in winter. This could be attributed to various factors such as sources of slaughtered animals, changes in management practice and ecological factors. The overall trend was relatively the same when annual condemnations were adjusted for the effect of seasonal variation. The present survey provides baseline data for the future monitoring of this potentially important parasitic disease in the region.     

Effects of drought stress on the seedling growth,development, and metabolic activity in different cultivars of canola

ABSTRACT

The comparative effects of drought stress on seed performance, growth parameters, free proline content, lipid peroxidation, and several antioxidative enzymes activities were studied in both cultivars of Brassica napus L. Drought stress increased mean germination rate and mean of day germination in both cultivars, and its effect was more pronounced in RGS003. The length and dry weight of root increased significantly in both cultivars under drought. RGS003 was more tolerant and obtained more biomass under drought than that of Sarigol. Proline content and pyrroline-5-carboxylate synthetase expression increased in Sarigol under drought. Measurement of malondialdehyde content in seedlings showed that lipid peroxidation was lower in RGS003 than in Sarigol. Antioxidant enzyme activities showed different trends in the two cultivars under drought stress but were higher in RGS003 than in Sarigol. These results suggest that RGS003 is better protected against drought-induced oxidative damage. Lipoxygenase activity only induced under water deficit condition in RGS003. Changes of respiratory enzymes activities of RGS003 subjected to drought stress showed a pattern different with Sarigol. Drought stress induced aconitase activity in RGS003, but it reduced fumarase and succinate dehydrogenase activity in Sarigol. This study showed that RGS003 exhibits a better protection mechanism against oxidative damage and RGS003 is more drought-tolerant than Sarigol possibly by maintaining and/or increasing growth parameters, antioxidant enzyme activities, and respiratory enzymes activities.     

Role of selenium in mitigation of cadmium toxicity in pepper grown in hydroponic condition

Selenium (Se) is an essential element for humans but is not considered as essential for plants. However, its beneficial role in improving plant growth and stress tolerances is well established. In order to study the role of Se in cadmium (Cd) toxicity in pepper (Capsicum frutescens cv. Suryankhi Cluster), this experiment was carried out in greenhouse conditions. Treatments comprised Cd [0, 0.25, and 0.5 mM cadmium chloride (CdCl₂)] and Se [0, 3, and 7 µM sodium selenite (Na₂SeO₃)] with three replications. The result showed that Cd decreased chlorophyll a, chlorophyll b, and carotenoids, whereas Se supplementation diminished Cd toxicity on photosynthetic pigment. Selenium at 7 µM significantly increased the leaf area in the plants grown at 0.25 mM Cd. The application of Se at 3 µM with 0.25 mM Cd and Se at 3 µM and Se at 7 µM with 0.5 mM Cd increased the activity of catalase (CAT). Selenium at 7 µM decreased the proline content of pepper leaves exposed to Cd at 0.5 mM (30%). Selenium significantly enhanced the antioxidant activity of leaves, which was diminished by Cd toxicity. In general, Se has a beneficial effect on plant growth and is an antioxidant enzyme of pepper cv. Suryankhi Cluster under Cd stress and non-stress conditions.     

Growth characteristics of Artemisia sieberi influenced by super absorbent polymers in texturally different soils under water stress condition

The effects of super absorbent polymers (SAPs) on growth characteristics and seedlings survival of Artemisia sieberi (under two soil textures, three irrigation levels and seven hydrogel compositions with three replications) were investigated. The studied traits were shoot height, shoot dry weight, root dry weight, ratio of root/shoot, root length, root perimeter, root area and root volume. SAPs successfully enhanced growth capability of A. sieberi in two soil textures compared to the controls. Ideally, 5 g kg^?1 Aquasorb? (SNF Company, France) with 100% irrigation and 10 g kg^?1 Stockosorb® (Evonik Corporation, Germany) with 75% irrigation in a sandy loam texture and 10 g kg^?1 Boloorab A? (Boloorab Company, Iran) with 75% irrigation in a loamy texture significantly affected all traits, resulting in 100% survival for A. sieberi seedlings. Aquasorb? and Stockosorb® showed the best results in the sandy loam texture and preferable outputs were obtained by Boloorab A? application in the loamy texture. In other words, because of the basic differentiation among soils in terms of mineralogy, temperature and moisture content, different SAPs should be applied. Production of dense root network and root aggregation stimulated by SAPs increased root contact with moisture. Therefore, improving the growth and survival of the plants is accessible using SAPs under water stress condition.     

Integrated effects of cattle manure-derived biochar and soil moisture conditions on soil chemical characteristics and soybean yield

ABSTRACT

A greenhouse experiment was carried out to evaluate the effect of 0, 25, 50 and 100 ton ha^?1 of cattle manure-derived biochar (BC) and soil moisture conditions (SMC) of 100%, 70% and 55% of water-holding capacity (WHC) on soybean production. Most of the macro and micronutrient, soil chemical characteristics and yield production of soybean were significantly affected by BC. Under drought, BC was more effective than that of the non-stressed conditions. Interaction effect of SMC and BC on soil iron (Fe), zinc (Zn), manganese (Mn), calcium (Ca), magnesium (Mg), phosphorus (P) and nitrogen (N) concentrations was also significant indicating that different levels of water supply might show different impact under different levels of BC. Under drought, the higher BC applied, the higher grain yield obtained. Application of 0 to 75 ton BC ha^?1 under WHC caused a high grain yield but 100 ton BC ha^?1 significantly decreased it as compared with the other levels of BC. Positive impacts of BC may be more pronounced under drought. High level of BC in non-stressed conditions may reduce grain yield as occurred in this study. The statistical techniques indicated that higher content of macro and micronutrients negatively associated with yield production of soybean.     

Effect of nitrogen and wheat residue on cotton (Gossypium hirsutum L.) yield and weed control

Wheat (Triticum aestivum L.) residues and nitrogen (N) management are the major problems in the southern part of Iran where irrigated wheat–cotton (Gossypium hirsutum L.)–wheat rotation is a common practice. A 2-year (2009–2011) field experiment was conducted as a split plot design with four replications at a cotton field (Darab), Fars Province, Iran, to determine the influence of different rates of wheat residue (0%, 25%, 50%, and 75%) incorporation and N rates (150, 200, 300, and 400 kg ha^?1) on weed suppression, yield, and yield components of cotton. Results showed that a higher residue incorporation and a lower N rate improved weed suppression in both years. For treatments receiving 150 kg N ha^?1 and 75% of wheat residues (2250 kg ha^?1), weed biomass and density were significantly lower compared to treatments receiving 400 kg N ha^?1. The highest cotton lint yield (about 2400–2700 kg ha^?1) was obtained by 300 kg N ha^?1 in the absence of residue application, in both years. Incorporation of 25% of wheat residue (750 kg ha^?1) and application of 300 kg N ha^?1 are recommended to guarantee an optimum level of cotton lint yield and weed suppression in a wheat–cotton–wheat rotation in this region.