全文获取类型
收费全文 | 1256篇 |
免费 | 76篇 |
专业分类
林业 | 54篇 |
农学 | 23篇 |
基础科学 | 3篇 |
219篇 | |
综合类 | 130篇 |
农作物 | 85篇 |
水产渔业 | 72篇 |
畜牧兽医 | 671篇 |
园艺 | 21篇 |
植物保护 | 54篇 |
出版年
2023年 | 11篇 |
2022年 | 13篇 |
2021年 | 20篇 |
2020年 | 28篇 |
2019年 | 37篇 |
2018年 | 29篇 |
2017年 | 32篇 |
2016年 | 32篇 |
2015年 | 23篇 |
2014年 | 32篇 |
2013年 | 58篇 |
2012年 | 74篇 |
2011年 | 107篇 |
2010年 | 37篇 |
2009年 | 41篇 |
2008年 | 81篇 |
2007年 | 72篇 |
2006年 | 69篇 |
2005年 | 83篇 |
2004年 | 84篇 |
2003年 | 75篇 |
2002年 | 58篇 |
2001年 | 18篇 |
2000年 | 9篇 |
1999年 | 8篇 |
1998年 | 10篇 |
1997年 | 11篇 |
1996年 | 10篇 |
1995年 | 10篇 |
1994年 | 13篇 |
1993年 | 7篇 |
1992年 | 11篇 |
1991年 | 10篇 |
1990年 | 5篇 |
1988年 | 5篇 |
1987年 | 4篇 |
1986年 | 8篇 |
1985年 | 6篇 |
1984年 | 6篇 |
1983年 | 6篇 |
1982年 | 5篇 |
1975年 | 5篇 |
1973年 | 3篇 |
1972年 | 6篇 |
1970年 | 4篇 |
1969年 | 4篇 |
1967年 | 4篇 |
1966年 | 6篇 |
1959年 | 4篇 |
1953年 | 4篇 |
排序方式: 共有1332条查询结果,搜索用时 15 毫秒
21.
Gui Xiao Frances Nikki Borja Ramil Mauleon Jonas Padilla Mary Jeanie Telebanco-Yanoria Jianxia Yang Guodong Lu Maribel Dionisio-Sese Bo Zhou 《Rice》2017,10(1):37
Background
The rice Pi2/9 locus harbors multiple resistance (R) genes each controlling broad-spectrum resistance against diverse isolates of Magnaporthe oryzae, a fungal pathogen causing devastating blast disease to rice. Identification of more resistance germplasm containing novel R genes at or tightly linked to the Pi2/9 locus would promote breeding of resistance rice cultivars.Results
In this study, we aim to identify resistant germplasm containing novel R genes at or tightly linked to the Pi2/9 locus using a molecular marker, designated as Pi2/9-RH (Pi2/9 resistant haplotype), developed from the 5′ portion of the Pi2 sequence which was conserved only in the rice lines containing functional Pi2/9 alleles. DNA analysis using Pi2/9-RH identified 24 positive lines in 55 shortlisted landraces which showed resistance to 4 rice blast isolates. Analysis of partial sequences of the full-length cDNAs of Pi2/9 homologues resulted in the clustering of these 24 lines into 5 haplotypes each containing different Pi2/9 homologues which were designated as Pi2/9-A5, ?A15, ?A42, ?A53, and -A54. Interestingly, Pi2/9-A5 and Pi2/9-A54 are identical to Piz-t and Pi2, respectively. To validate the association of other three novel Pi2/9 homologues with the blast resistance, monogenic lines at BC3F3 generation were generated by marker assisted backcrossing (MABC). Resistance assessment of the derived monogenic lines in both the greenhouse and the field hotspot indicated that they all controlled broad-spectrum resistance against rice blast. Moreover, genetic analysis revealed that the blast resistance of these three monogenic lines was co-segregated with Pi2/9-RH, suggesting that the Pi2/9 locus or tightly linked loci could be responsible for the resistance.Conclusion
The newly developed marker Pi2/9-RH could be used as a potentially diagnostic marker for the quick identification of resistant donors containing functional Pi2/9 alleles or unknown linked R genes. The three new monogenic lines containing the Pi2/9 introgression segment could be used as valuable materials for disease assessment and resistance donors in breeding program.22.
Context
Spatial heterogeneity has myriad influences on ecosystem processes, ecosystem services, and thus the sustainability of urban areas. It acts as a medium for urban design, planning, and management to determine how processes affecting sustainability can operate and interact. Therefore, how spatial heterogeneity is conceptualized and measured in cities is crucial for enhancing sustainability.Objectives
We show that the two most commonly used, but contrasting paradigms of urban ecology, ecology IN versus ecology OF the city, determine how spatial heterogeneity is thought of and used in different ways. We identify the key implications of these theoretical contrasts for the practice and assessment of sustainability in urban areas.Methods
We review and compare the different ways in which ecology IN versus ecology OF the city affect how to conceptualize, model and map urban spatial heterogeneity. We present a new framework to guide the comparison of spatial heterogeneity under the two paradigms.Results and conclusion
The integrative nature of this new framework becomes apparent under the ecology OF the city paradigm, because it recognizes the hybrid social and bioecological nature of heterogeneity in urban ecosystems. The hybrid approach to patchiness resonates with the three pillars of sustainability—environment, society, and economy. We exemplify how the more comprehensive and integrated framework of spatial heterogeneity under the ecology OF the city paradigm (1) supports more effective measurement and integration of the three components of sustainability, (2) improves management of heterogeneous urban ecosystems, and (3) satisfies calls for improved ecological tools to support urban ecosystem design.23.
Paige E Boyle Michael D Richardson Mary C Savin Douglas E Karcher Daniel A Potter 《Pest management science》2019,75(8):2071-2078
Earthworm activity is beneficial in most natural and agricultural systems, but excessive earthworm casting is a problem on sports fields worldwide. Expulsion of soil‐rich earthworm fecal matter, or casts, as mounds of soil on the turf canopy can muddy the surface, reduce photosynthesis, and lead to thinning, weed invasion and surface softening. Casts affect ball roll on sports fields, cause fouling of maintenance equipment, and dull mower blades. Build‐up of cast material on reel mower units can affect height and quality of cut. Casting activity is dependent on environmental conditions such as soil texture, temperature, moisture, pH, and organic matter. Response to environmental conditions varies by species. Management options are limited, because no pesticides are specifically labeled for earthworm control at this time, and cultural control methods such as soil modification, turfgrass clipping removal, and sand topdressing have limited and inconsistent efficacy. Products containing plant‐derived saponins and irritants show promise for earthworm management. Pest management practices to mitigate excessive earthworm casting will likely need to be species‐specific, but limited knowledge of earthworm identification by end‐users further inhibits the efficacy of control measures. © 2019 Society of Chemical Industry 相似文献
24.
Predicting the impact of irrigation practices on soil N mineralization and N balance is an important issue to optimize N fertilization
and reduce the N losses towards the environment. The effect of summer irrigation on N dynamics was investigated in two arable
fields in Southern France. Net N mineralization was assessed by combining frequent measurements of water and mineral N contents
in soil and the use of a calculation model (LIXIM). It was first calculated assuming that denitrification was negligible.
This hypothesis led to inconsistent results, apparent net N mineralized being smaller under irrigated than non-irrigated conditions
and net mineralization kinetics being erratic. The occurrence of denitrification was confirmed by the use of 15NO3 tracing in an experiment carried out in summer, including three irrigated treatments. The average 15N recovery varied from 45% to 85% and was smallest in the most frequently irrigated treatment. Over the 8-week experiment,
the N losses varied from 30 to 38 kg ha−1 in the irrigated treatments. They were satisfactorily simulated by a simple denitrification model (NEMIS). Combining the
LIXIM model and the simulated or calculated denitrification allowed to predict satisfactorily the evolution of soil mineral
N accounting for the effects of temperature and moisture. The net N mineralized for 8 weeks varied from 34 kg N ha−1 in the un-irrigated to 46 kg N ha−1 in the irrigated treatments. The drying–rewetting cycles did not induce a flush of N mineralization. Our results suggest
that denitrification has to be accounted for in irrigated systems, particularly in warm conditions and when the topsoil contains
high nitrate contents. 相似文献
25.
Bradley D Ohlinger Roger Schürch Sharif Durzi Parry M Kietzman Mary R Silliman Margaret J Couvillon 《Journal of insect science (Online)》2022,22(1)
Honey bees (Linnaeus, Hymenoptera: Apidae) are widely used as commercial pollinators and commonly forage in agricultural and urban landscapes containing neonicotinoid-treated plants. Previous research has demonstrated that honey bees display adverse behavioral and cognitive effects after treatment with sublethal doses of neonicotinoids. In laboratory studies, honey bees simultaneously increase their proportional intake of neonicotinoid-treated solutions and decrease their total solution consumption to some concentrations of certain neonicotinoids. These findings suggest that neonicotinoids might elicit a suboptimal response in honey bees, in which they forage preferentially on foods containing pesticides, effectively increasing their exposure, while also decreasing their total food intake; however, behavioral responses in semifield and field conditions are less understood. Here we conducted a feeder experiment with freely flying bees to determine the effects of a sublethal, field-realistic concentration of imidacloprid (IMD) on the foraging and recruitment behaviors of honey bees visiting either a control feeder containing a sucrose solution or a treatment feeder containing the same sucrose solution with IMD. We report that IMD-treated honey bees foraged less frequently (–28%) and persistently (–66%) than control foragers. Recruitment behaviors (dance frequency and dance propensity) also decreased with IMD, but nonsignificantly. Our results suggest that neonicotinoids inhibit honey bee foraging, which could potentially decrease food intake and adversely affect colony health. 相似文献
26.
27.
28.
Geoffrey G. Briggs George R. Cayley Glenn W. Dawson David C. Griffiths Ewen D. M. Macaulay John A. Pickett Mary M. Pile Lester J. Wadhams Christine M. Woodcock 《Pest management science》1986,17(4):441-448
Two analogues of the aphid alarm pheromone (E)-β-farnesene, a trifluorofarnesene and a difluoro-1-norfarnesene, were found to be highly active and were more readily detectable than the parent compound. For (Z)-hexadec-11-enal, a component of some lepidopteran sex attractant pheromones, replacement of the carbonyl oxygen with a difluoromethyl group to give a difluoroheptadecadiene resulted in loss of activity. A trifluoroacetoxyhexadecanolide was a more volatile analogue of the mosquito oviposition pheromone (?)-(5R,6S)-6-acetoxy-5-hexadecanolide and was highly active. 相似文献
29.
Luis R Padilla Christopher J Dutton Joan Bauman Mary Duncan 《Journal of zoo and wildlife medicine》2005,36(3):498-503
A 2-yr-old Arabian oryx (Oryx leucoryx) was presented for evaluation of abnormal genitalia and infantile behavior. The oryx had a penis and a scrotum, but testes were not palpable within the scrotum or inguinal canal. The total serum testosterone for the individual was lower than in age-matched males of the same species. Surgical exploration showed markedly hypoplastic intra-abdominal gonads, which demonstrated both testicular and uterine tissue on histologic examination. After karyotype analysis, the individual was classified as an XY male pseudohermaphrodite. This condition resembles two human intersex syndromes: embryonic testicular regression syndrome and partial gonadal dysgenesis syndrome, which occur in familial lines. 相似文献
30.
Riitta Nissinen Shaaban Kassuwi Riikka Peltola Mary C. Metzler 《European journal of plant pathology / European Foundation for Plant Pathology》2001,107(2):175-182
Clavibacter michiganensis subsp. sepedonicus, a Gram positive bacterium that causes bacterial ring rot of potato, was studied in eggplant, an alternate host, using strains that differed in phenotype. Two factors affecting virulence, the ability to induce a hypersensitive response (HR) and cellulase production, were studied. A plasmid-free isolate of C. michiganensis subsp. sepedonicus that causes HR on tobacco but is unable to produce cellulase multiplied efficiently in planta, but caused only weak symptoms. In contrast, a strain that is unable to induce HR on tobacco but produces cellulase was impaired in the ability to multiply in the host and caused no symptoms. When the two non-virulent strains were coinoculated into eggplants, typical disease symptoms developed. This enhancement was not due to formation of a new phenotype or significant increases in population density of either of the strains. Our results suggest that both cellulase production and the ability to induce HR are required for a successful infection process and disease induction by C. michiganensis subsp. sepedonicus. Our results additionally suggest that the ability to induce HR on non-host plants is required for multiplication in the host plant, whereas cellulase expression is necessary for induction of disease symptoms. 相似文献