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31.
Michael W. Mullowney Eoghainín ó hAinmhire Anam Shaikh Xiaomei Wei Urszula Tanouye Bernard D. Santarsiero Joanna E. Burdette Brian T. Murphy 《Marine drugs》2014,12(6):3574-3586
As part of our program to identify novel secondary metabolites that target drug-resistant ovarian cancers, a screening of our aquatic-derived actinomycete fraction library against a cisplatin-resistant ovarian cancer cell line (OVCAR5) led to the isolation of novel diaza-anthracene antibiotic diazaquinomycin E (DAQE; 1), the isomeric mixture of diazaquinomycin F (DAQF; 2) and diazaquinomycin G (DAQG; 3), and known analog diazaquinomycin A (DAQA; 4). The structures of DAQF and DAQG were solved through deconvolution of X-Ray diffraction data of their corresponding co-crystal. DAQE and DAQA exhibited moderate LC50 values against OVCAR5 of 9.0 and 8.8 μM, respectively. At lethal concentrations of DAQA, evidence of DNA damage was observed via induction of apoptosis through cleaved-PARP. Herein, we will discuss the isolation, structure elucidation, and biological activity of these secondary metabolites. 相似文献
32.
Cotton?K.?RandallEmail author Mary?L.?Duryea Susan?W.?Vince R.?Jeffery English 《New Forests》2005,29(3):245-260
Sprouting can be an important mechanism of regeneration in harvested cypress (Taxodium sp.) wetlands; however, limited information exists on factors influencing sprouting. We examined the influence of harvesting practices, tree size, and site conditions on stump sprouting in eight pondcypress (Taxodium distichum var. nutans (Ait.) Sweet) wetlands in Florida, USA. Stumps were cut at three heights: 20 cm below high water level, at high water, and 40 cm above high water. Half of each site was harvested using a sawhead feller and the other half using shearhead. We monitored sprouting for 2 years post-harvest. Stump height affected first year sprouting, but the effect disappeared during year 2. Felling equipment did not affect sprouting; however, the shearhead caused greater stump damage. Sprouting declined with increasing parent tree size; percentage of stumps with sprouts after 2 years was 68% for trees 10–14 cm dbh and 28% for trees 20–24 cm dbh. Stump sprouting ranged among sites from 23 to 54% after 2 years. Results indicate that sprouting may be an important, but inadequate, form of regeneration. At sites with short hydroperiods, dense understories, and/or predominance of trees greater than 20 cm dbh, reduced sprouting may occur, and planting seedlings may be beneficial. 相似文献
33.
34.
Summary Plants were grown from seed of two-rowed wild barley, Hordeum spontaneum, and six-rowed brittlerachised barley. H. agriocrithon, collected in Ladakh, north-western India. Whereas the H. spontaneum remained true to type, segregation for morphological characters was observed in progeny rows grown from heads of H. agriocrithon plants. The H. agriocrithon heads also showed segregation for a biochemical character, the polypeptide pattern of the endosperm storage protein fraction (hordein). The H. agriocrithon seed therefore originated from natural hybridization between cultivated H. vulgare and weedy H. spontaneum. Crosses of H. vulgare and H. spontaneum gave progeny which resembled H. agriocrithon and showed similar hordein polypeptide segregation patterns. The results indicate that six-rowed brittle-rach ised barleys from the Himalayas have a similar origin to forms found in the Middle East, and that H. agriocrithon does not play a direct role in the evolution of barley. 相似文献
35.
Kim N. Irvine Mary F. Perrelli Ratchadawan Ngoen-klan Ian G. Droppo 《Journal of Soils and Sediments》2009,9(4):328-341
Background, aim and scope Street sediment samples were collected at 50 locations in a mixed land use area of Hamilton, Ontario, Canada, and metal levels
were analyzed using a sequential extraction procedure for different particle size classes to provide an estimate of potential
toxicity as well as the potential for treatment through best management practices (BMPs).
Methodology The street sediment samples were dry sieved into four different particle size categories and a sequential extraction procedure
was done on each size category following the methodology proposed by Tessier et al. 1979 using a Hitachi 180-80 Polarized Zeeman Atomic Absorption Spectrophotometer.
Results and discussion Analysis of variance, post hoc least-significant difference tests, and kriging analysis showed that spatially Mn and Fe levels
were associated with a well-defined heavy industrial area that includes large iron- and steel-making operations; Cu and Pb
were associated with both the industrial and high-volume traffic areas, while Zn tended to be more associated with high-volume
traffic areas. The potential bioavailability of the metals, based on the sum of chemical fractions 1 (exchangeable) and 2
(carbonate-bound), decreased in order: Zn > Cd > Mn > Pb > Cu > Fe. Based on aquatic sediment quality guidelines, there is
some concern regarding the potential impact of the street sediment when runoff reaches receiving waters.
Conclusions It is possible that a combination of BMPs, including street sweeping and constructed wetlands, could help to reduce street
sediment impact on environmental quality in the Hamilton region. The data presented here would be important in developing
and optimizing the design of these BMPs. 相似文献
36.
Soil organic carbon (SOC) has considerable spatial and temporal variability both at the hillslope and catchment scale as well as down the soil profile. In recent years the distribution of SOC down the soil profile has become an area of interest in the understanding of the carbon sequestration potential of soils. Most studies however have concentrated on highly disturbed agricultural sites with little data available for untilled locations. In this study the vertical distribution of SOC is examined at a grassland site in the Young River area of Western Australia that has remained undisturbed by human activity for 50 years. Soil physical properties (texture, rock content) as well as the distribution of the environmental tracers 137Cs and 210Pb were assessed with the aim of better understanding the transport processes which produce the observed vertical distribution of SOC. While no consistent relationship was found between SOC and soil physical properties significant relationships were found between the distribution of SOC and the environmental tracers, 137Cs and 210Pb. Finite element simulations based on a diffusion/convection/decay model showed that the transport of 137Cs and 210Pb down the soil profile is likely to be driven by the same (primarily diffusive) processes. The same model used in conjunction with plant input and decay data generated from the RothC-26.3 soil carbon model revealed that transport of SOC down the soil profile, while also a diffusion process, was significantly slower indicating that different processes and/or pathways are involved in SOC transport at this site. 相似文献
37.
Mary R. Arkoosh Joseph P. Dietrich Mary Beth Rew Wendy Olson Graham Young Frederick W. Goetz 《Aquaculture Research》2018,49(1):205-216
Wild sablefish, Anoplopoma fimbria, are a valuable commercial species whose populations are declining. Fortunately, sablefish are excellent species for commercial aquaculture. Sablefish raised under high‐density conditions in the marine environment require the use of efficacious vaccines to control disease. Sablefish impacted by disease in net pens may have poor flesh quality and high mortality during grow‐out. As a result, disease can cause financial hardship for sablefish aquaculture operators. The efficacy of a multivalent vaccine preparation for sablefish, administered either by intraperitoneal (i.p.) injection or by immersion, against atypical and typical Aeromonas salmonicida, the causative agents of atypical and typical furunculosis, respectively, was examined. A. salmonicida can affect sablefish at any age and size. Consequently, an efficacious vaccine that can be appropriately and optimally administered to all life stages is desirable. Sablefish vaccinated by immersion at ~1.5 or ~4.5 g with a whole‐cell multivalent vaccine were not protected against either typical or atypical A. salmonicida. Factors that may have contributed to the ineffectiveness of the immersion vaccine are discussed. By contrast, the relative per cent survival (RPS) or potency of the whole‐cell multivalent vaccine injected i.p. in juvenile sablefish at ~50 g against typical and atypical A. salmonicida was 94.3% and 81.7% respectively. The high RPS values indicated that the vaccine successfully initiated an immune response in sablefish upon a second encounter with the pathogen. 相似文献
38.
Adriana Vella Sinéad Murphy Joan Giménez Renaud de Stephanis Barbara Mussi Joseph G. Vella Kamel Larbi Doukara Daniela Silvia Pace 《水产资源保护:海洋与淡水生态系统》2021,31(Z1):110-136
- Preservation of endangered species is now recognized as a relevant component of regional and global conservation actions achieved through the maintenance of the remaining populations in a favourable conservation status. The Mediterranean common dolphin (Delphinus delphis) is listed as an Endangered subpopulation in the International Union for Conservation of Nature Red List, with declining numbers in some of the areas where it has been studied for numerous years. Prey depletion, fisheries bycatch, pollution, health risks, and climate change are among the recognized threats that the species is facing in the region.
- The existing national, regional, and international legislation pertinent to Mediterranean countries should aid greater science–policy–management integration and support innovative research and monitoring towards effective conservation. The challenges inherent in studying the pelagic D. delphis should not preclude the use of long-term, year-round, multiplatform surveys, side by side with the latest molecular tools, which aid our understanding of its populations' genetic diversity, resistance to diseases, and resilience in a semi-enclosed fast-changing sea.
- Addressing the multiplicity of problems confronting the species requires collaborative effort at all levels to share and merge resources, data, and expertise more efficiently. In this review, we provide an overview of the current knowledge on the Mediterranean common dolphin, the main threats, and knowledge gaps that are priorities for addressing its conservation, while providing clear recommendations for effectively safeguarding the species and its habitat at both local and regional levels.
39.
Simons AL Renouf M Hendrich S Murphy PA 《Journal of agricultural and food chemistry》2005,53(10):4258-4263
The relationship between chemical structure and gut microbial degradation rates of 14 flavonoids, flavone, apigenin, chrysin, naringenin, kaempferol, genistein, daidzein, daidzin, puerarin, 7,4'-dihydroxyflavone, 6,4'-dihydroxyflavone, 5,4'-dihydroxyflavone, 5,3'-dihydroxyflavone, and 4'-hydroxyflavone, was investigated by anaerobically fermenting the flavonoids with human gut microflora (n = 11 subjects). Degradation rates for the 5,7,4'-trihydroxyl flavonoids, apigenin, genistein, naringenin, and kaempferol, were significantly faster than the other structural motifs. Puerarin was resistant to degradation by the gut microflora. Extensive degradation of flavonoids by gut microflora may result in lower overall bioavailability than those flavonoids that are slowly degraded because rapidly degrading flavonoids are less likely to be absorbed intact. 相似文献
40.
Eileen A. Murphy John Dooley Herbert L. Windom Ralph G. Smith Jr. 《Water, air, and soil pollution》1994,78(1-2):61-72
Water samples from 78 private potable wells in southern New Jersey were collected for mercury analysis in 1991–1992. Analyses were performed for the quantification of reactive, volatile and methyl mercury species. Relationships of mercury with other water quality parameters were evaluated also. Total mercury concentrations varied from <1 ng L?1 to over 36 Μg L?1. The dominant forms in which mercury occurred in the wells sampled were Hg? and HgCl2?, although methyl mercury was present in some wells and comprised up to 8% of the total mercury in the ground water samples. 相似文献