Interspecific hybridization between Atlantic salmon and brown trout introduced in the subantarctic Kerguelen Islands

In the subantarctic French territory of the Kerguelen Islands, Atlantic salmon Salmo salar was stocked to create a new population in the Korrigans drainage system. The analysis of historical scale collections showed that hybrids were inadvertently introduced with the original Atlantic salmon stocks. Later colonization of the Korrigans system by brown trout Salmo trutta was followed by interspecific hybridization between the two species. Our results highlight the importance of both routine genetic monitoring of hatchery stocks and considering the possible influence of other species when stocking is envisaged.     

Liquid chromatographic determination of tetracycline residues in animal feeds

A liquid chromatographic method for the multiresidue determination of tetracyclines (TCs) in feeds is described. The levels of quantitation were 10 ppm each for tetracycline-HCl (TC), oxytetracycline (OTC), and chlortetracycline-HCl (CTC); the detection limit was 40 ppb for each. The calibration curves were linear between 2.5 and 100 ppm. The procedure involved double extraction with pH 2.0 and pH 4.5 McIlvain buffers, cleanup on a Sephadex LH-20 column, separation on a Nova-Pak C18 column, and detection at 370 nm. Recoveries of 10 micrograms/g of each TC in multiresidue feed samples ranged from 55.8 to 75.5% for OTC, 71.6 to 100% for TC, and 22.4 to 60.6% for CTC. The identities of the TCs were confirmed by thin layer chromatography.     

Juice and phenolic fractions of the berry Aristotelia chilensis inhibit LDL oxidation in vitro and protect human endothelial cells against oxidative stress

Oxidative modification of low-density lipoprotein (LDL) particles is a key event in the development of atherosclerosis. Oxidized LDL induces oxidative stress and modifies gene expression in endothelial cells. Berries constitute a rich dietary source of phenolic antioxidants. We found that the endemic Chilean berry Aristotelia chilensis (ach) has higher phenol content and scores better for total radical-trapping potential and total antioxidant reactivity in in vitro antioxidant capacity tests, when compared to different commercial berries. The juice of ach is also effective in inhibiting copper-induced LDL oxidation. In human endothelial cell cultures, the addition of ach juice significantly protects from hydrogen peroxide-induced intracellular oxidative stress and is dose-dependent. The aqueous, anthocyanin-rich fraction of ach juice accounts for most of ach's antioxidant properties. These results show that ach is a rich source of phenolics with high antioxidant capacity and suggest that it may have antiatherogenic properties.     

Bioactive compounds in cod (Gadus morhua) products and suitability of 1H NMR metabolite profiling for classification of the products using multivariate data analyses

This work investigates the suitability of (1)H NMR spectroscopy to identify the fate of some bioactive compounds in seafood submitted to several processing conditions and examines the possibility of using (1)H NMR spectroscopy profiling to classify such products. Perchloric acid extracts of cod white muscle from newly killed and (i) unprocessed, (ii) boiled, and (iii) fried fillets and from (iv) frozen fillets, (v) the frozen fillets after thawing, and (vi) their drip loss and from (vii) rehydrated cod klippfish (n = 5) were analyzed by 500 MHz (1)H NMR spectroscopy. It was possible to identify taurine, betaine, anserine, creatine, and trimethylamine oxide (TMAO) in all extracts examined, and frozen fish was recognizable by the presence of dimethylamine (DMA). None of the heating procedures seemed to induce the loss of bioactive compounds from the fillet, but freezing and thawing did: the compounds were lost in what is known as drip loss. About 80% of the samples were correctly classified using a probabilistic neural network procedure having as inputs the scores of the first 20 principal components of the principal component analysis of a selected region of the NMR spectra.     

Microfungal biomass and number of propagules in an andosol

The microfungal community of an andosol, under an acid beech forest located in a zone of the Province of Navarra (Spain) characterized by a temperate climate and high rainfall was studied. Mycelial length, number of spores and fungal biomass were eslimated by the agar-film technique. The number of fungal propagules were estimated by plate counting. There was a pronounced decrease of fungal propagules and mycelial biomass with soil depth; the highest values were obtained in the spring and the autumn. Seasonal variations were attenuated with increasing depth. In autumn the leaves of the A₀₀ horizon contained a great number of fungal spores but there was little mycelial growth.     

The larch wood pasture: structure and dynamics of a cultural landscape

As a consequence of land abandonment and marginalization, open areas and traditionally managed wood pastures are disappearing from many alpine valleys. Landscape and stand scale dynamics were analyzed in two neighboring inner valleys of central Italian Alps (Valmalenco, SO) dominated by larch (Larix decidua Mill.) forests and experiencing different historical land use intensities. Land cover mapping obtained from object-oriented analysis of aerial photographs was used to quantify land cover changes between 1961 and 2003. Spatial statistics were used to quantify landscape patterns, and intensive sampling in permanent plots was employed to perform point pattern analysis at the stand scale. Expansion of forested area at the expense of wood pastures was the main land cover transition observed. Differences in land use intensities were responsible for different patterns at the landscape scale: (1) heavier grazing and human pressure created a more diverse and fragmented mosaic of meadows, open woodlands, forests and wood pastures, (2) lower human impact favored a more homogeneous and stable system. A decline in grazing pressure in the late 1970s favored the establishment of larch and spruce in a clustered pattern, typical of the subalpine belt. Subalpine wood pastures, likewise many other semi-natural environments, are in danger of disappearing and lose their productive and cultural characteristics, because they are developing into more closed and dense stands.     

A basal dinosaur from the dawn of the dinosaur era in southwestern Pangaea

Upper Triassic rocks in northwestern Argentina preserve the most complete record of dinosaurs before their rise to dominance in the Early Jurassic. Here, we describe a previously unidentified basal theropod, reassess its contemporary Eoraptor as a basal sauropodomorph, divide the faunal record of the Ischigualasto Formation with biozones, and bracket the formation with (40)Ar/(39)Ar ages. Some 230 million years ago in the Late Triassic (mid Carnian), the earliest dinosaurs were the dominant terrestrial carnivores and small herbivores in southwestern Pangaea. The extinction of nondinosaurian herbivores is sequential and is not linked to an increase in dinosaurian diversity, which weakens the predominant scenario for dinosaurian ascendancy as opportunistic replacement.