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101.
Apicomplexan parasites such as Toxoplasma gondii and Plasmodium species actively invade host cells through a moving junction (MJ) complex assembled at the parasite-host cell interface. MJ assembly is initiated by injection of parasite rhoptry neck proteins (RONs) into the host cell, where RON2 spans the membrane and functions as a receptor for apical membrane antigen 1 (AMA1) on the parasite. We have determined the structure of TgAMA1 complexed with a RON2 peptide at 1.95 angstrom resolution. A stepwise assembly mechanism results in an extensive buried surface area, enabling the MJ complex to resist the mechanical forces encountered during host cell invasion. Besides providing insights into host cell invasion by apicomplexan parasites, the structure offers a basis for designing therapeutics targeting these global pathogens.  相似文献   
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103.
Forestry practices such as fuel-reduction burning and maintenance of road networks can negatively impact avian assemblages, both directly by changing habitat structure and indirectly by creating conditions favorable for predators or competitors. The Brigalow Belt forests include some of the largest contiguous areas of native forest in the temperate and sub-humid zones of eastern Australia. Over 1 million ha of these forests are proposed to be converted from forestry to conservation tenure, yet the impacts on the avifauna of current and potential future forest management practices are not known. We investigated the influence of road edges and habitat type and structure on the avifauna of a 356 000 ha forest. Survey sites were either <50 m or >300 m from a road, and in either cypress pine Callitris glaucophylla forest, spotted gum Corymbia citriodora forest with a regenerating cypress pine/buloke Allocasuarina luehmannii understorey or spotted gum forest with an open understorey due to fuel-reduction burning. The avifauna differed significantly among vegetation types but not with proximity to a road, with the greatest differences between cypress pine and both types of spotted gum forest. The noisy miner Manorina melanocephala, an aggressive avian competitor, appears to be the factor mediating these assemblage-level differences. Noisy miners were rare in cypress pine forest but were three times more numerous than any other species in spotted gum forest. Spotted gum forest with a regenerating understorey had fewer noisy miners. Although total bird abundance was highest in open spotted gum forest, the species richness and abundance of passerines smaller than noisy miners was significantly lower in this forest type. Abundance of small passerines was eight times higher in sites where <3 noisy miners were recorded. Only one species, the eastern yellow robin, was influenced by proximity to a road edge. Cypress pine forest is potentially an important refuge for smaller birds. The results suggest that burning regimes that reduce regeneration of the cypress pine and buloke subcanopy in spotted gum forest potentially are exacerbating the problem of noisy miner domination of the avifauna.  相似文献   
104.
The growing number of honey bee colonies and beekeepers in Canada has led to a great diversity of beekeeping practices. All beekeeping operations, however, need to implement consistent management measures for the control of diseases. The objective of this study was to document the actual disease management practices of beekeeping productions in southwestern Quebec, Canada. A survey was conducted to describe management practices used by 15 beekeepers who own 1824 colonies in that area. Data were obtained by telephone interviews. When infectious diseases were suspected, beekeepers generally avoided using potentially toxic acaricides and chemical treatments associated with antimicrobial resistance and instead used preventive, physical or management methods, although laboratory diagnosis was rarely used. This study highlights the wide variety of operation sizes, activities, and disease management strategies among beekeepers in southwestern Quebec. It identifies the need to encourage the use of services available to them and to propose a standardized preventive medical approach for field veterinarians to avoid the spread of infectious diseases.  相似文献   
105.
The occurrence of twelve chlorinated and brominated volatile compounds was investigated by a HRGC (High Resolution Gas Chromatography) device with a dynamic purge (PTI, Chrompack) in the water of the river Seine under dry conditions and under contrasting hydrological regimes, according to three longitudinal profiles from upstream to downstream of the Paris urban centre. Our results showed an obvious rise of the solvent concentrations in the river Seine water along the profiles. The total chlorinated solvent concentration, which was below 1 μg L-1 upstream of Paris, reached a maximum value of 8 μg L-1 dowstream of the urban centre in December 1994. This corresponded to a flux of around 300 kg d-1. This contamination originated in successive waste inputs, both domestic and industrial, from the Paris main sewers. The losses by evaporation from the river were taken into account for the estimation of the waste. The waste inputs represented about 85 to 97% of the total chlorinated solvent flux observed downstream of Paris. During the two first sampling sessions, dichloromethane represented 67% of the inputs. Though the hydrological regime plays a part both in the waste dilution and in the losses by evaporation processes, other factors such as the industrial activities or the ambient temperature may have a major influence upon the river contamination.  相似文献   
106.
1. The influence of in-feed lactoferrin (Lf) on bird production, intestinal microbiota, mucosal immune system and gut microarchitecture was assessed in male Cobb 500 broiler chickens.

2. Birds were given one of four diets from day of hatch: Control (basal diet with no additives), ZnB (basal diet?+?50?mg/kg zinc bacitracin), Lf 250?mg/kg (basal diet?+?250?mg/kg Lf) and Lf 500?mg/kg (basal diet?+?500?mg/kg Lf); n?=?24 birds/treatment. An apparent metabolisable energy study was performed between d 25-32.

3. Lf did not affect growth rate or feed conversion in the period 0-21?d of age, nor performance or energy metabolism during the 7?d metabolism experiment which commenced at 25?d of age.

4. The profiles of caecal microbial communities were significantly different in birds given ZnB compared with birds given a diet with no additives, or supplemented with 250?mg/kg Lf. Birds given 250?mg/kg Lf also had a different microbial profile compared with birds given 500?mg/kg Lf.

5. In comparison to control birds, Lf treated birds showed some differences in the T cell proportions in caecal tonsil and spleen. No differences in ileal villus height, crypt depth or goblet cell proportions were observed amongst dietary treatments.

6. Whilst Lf had little effect on the measured parameters, the use of an integrated approach to study the influence of novel feed additives may facilitate a greater understanding of the relationships between nutrition, gut health and bird performance.  相似文献   

107.
Fish oils extracted from marinated herring (frozen and unfrozen) byproducts and maatjes herring byproducts were evaluated on their chemical and sensory properties. The obtained crude oils had very low content of copper (<0.1 mg/kg oil), and iron values were 0.8, 0.1, and 0.03 mg/kg oil, respectively, for oil from maatjes and frozen and fresh byproducts. For the maatjes oil, a much lower value was found for alpha-tocopherol compared to the other oils. Storage stability results showed that the oils behave differently. Secondary oxidation products were measured for fresh oil, while for the maatjes and frozen byproducts' oil, tertiary oxidation products were detected. Over storage time, the maatjes and frozen byproducts' oils became more intense in odor, correlating positively at the end with sensory attributes of train-oil, acidic, marine and fishy. The best correlation between sensory and chemical analyses was found for FFA and fishy off-odor (r = 0.781).  相似文献   
108.
109.
In the Lower Mekong Basin, paddy fields often appear as mosaics, with soil mounds covered by trees or other plants in a spotty distribution. These soil mounds are commonly named termite ‘lenticular mounds’ because termite bioturbation is considered to be at their origin. Termite mounds host a large diversity of animals and plants, increasing landscape patchiness. Because the preservation of these islands of biodiversity is threatened by modern agricultural practices, the aim of this study was to quantify their abundance and the services they provide to the local population. The abundance of mounds and their use by the population were quantified in a catchment in Cambodia. We found that mounds density reached ~2 mounds ha−1. Interviews carried out within the catchment showed that most of the interviewees used mounds for increasing the fertility of their field and for the cultivation of rice and other plants (e.g. sponge gourd and pumpkin). In addition to their potential to increase plant productivity, the survey revealed that animals (rats and snakes), mushrooms and 13 plant species found on or in mounds were consumed by the population. In addition to potentially contributing to an increase in food diversity, mounds also impacted farmers' health by allowing access to 20 medicinal plant species and indirectly via a reduction in pesticide use. In conclusion, this study is the first attempt to quantify the large number of services provided by termite mounds in Cambodia. This increase in the knowledge of the diversity of environmental and socioeconomic services provided by termite mounds is likely to contribute to their preservation and provide a basis for the sustainable management of biodiversity in paddy fields in the Lower Mekong Basin region.  相似文献   
110.
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