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11.
Blume E Bischoff M Moorman TB Turco RF 《Journal of agricultural and food chemistry》2004,52(24):7382-7388
Understanding the dissipation rates of chemicals in unsaturated and saturated zones of subsurface soils will help determine if reductions of concentrations to acceptable levels will occur. Chemical properties and microbial biomass and activity were determined for the surface (0-15 cm), lower root (50-105 cm), and vadose (175-220 cm) zones in a Huntington silty clay loam (Fluventic Hapludoll) collected from an agricultural field near Piketon, OH. The rates of sorption, mineralization, and transformation (formation of bound residues and metabolites) of atrazine were determined. Microbial activity was estimated from the mineralization of (14)C-benzoate. We observed decreased levels of nutrients (total organic carbon, N, and P) and microbial biomass with depth, while activity as measured with benzoate metabolism was higher in the vadose zone than in either the surface or the root zones. Sorption coefficients (K(f)) declined from 8.17 in the surface to 3.31 in the vadose zone. Sorption was positively correlated with organic C content. Rates of atrazine mineralization and bound residues formation were, respectively, 12-2.3-fold lower in the vadose than in the surface soil. Estimated half-lives of atrazine ranged from 77 to 101 days in the surface soil, but increased to over 900 days in the subsurface soils. The decreased dissipation of atrazine with increasing depth in the profile is the result of decreased microbial activity toward atrazine, measured either as total biomass or as populations of atrazine-degrading microorganisms. The combination of reduced dissipation and low sorption indicates that there is potential for atrazine movement in the subsurface soils. 相似文献
12.
Negligible contribution from roots to soil-borne phospholipid fatty acid fungal biomarkers 18:2ω6,9 and 18:1ω9 总被引:1,自引:0,他引:1
The phospholipid fatty acid biomarkers 18:1ω9, 18:2ω6,9 and 18:3ω3,6,9 are commonly used as fungal biomarkers in soils. They have, however, also been found to occur in plant tissues, such as roots. Thus, the use of these PLFAs as fungal biomarkers in sieved soil, which may still contain small remains of roots, has been questioned. We used data from a recent beech tree girdling experiment to calculate the contribution of roots to these biomarkers and were able to demonstrate that not more than 0.61% of 18:1ω9 and 18:2ω6,9 in sieved soil samples originated from roots (but 4% of 18:3ω3,6,9). Additionally, the abundance of the biomarker 18:2ω6,9 in the soil was found to be highly correlated to ectomycorrhizal root colonization, which further corroborates its fungal origin. PLFA biomarkers were substantially reduced in vital roots from girdled trees compared to roots of control trees (by up to 76%), indicating that the major part of PLFAs measured in roots may actually originate from ectomycorrhizal fungi growing inside the roots. We calculated, that even a near to 50% reduction in fine root biomass - as observed in the girdling treatment - accounted for only 0.8% of the measured decrease of 18:2ω6,9. Our results demonstrate that both 18:1ω9 and 18:2ω6,9 are suitable biomarkers for detecting fungal dynamics in soils and that especially 18:2ω6,9 is a reliable biomarker to study mycorrhizal dynamics in beech forests. 相似文献
13.
In order to meet the demands of modern, information-based society, higher education in natural resource management needs to be transformed. Enhanced mobility of students and staff, multi- and transdisciplinary approaches, as well as innovative educational approaches are called for. Moreover, the urban component needs to be better incorporated, as exemplified by the emerging field of urban forestry. This paper relates to the developments in higher education within the context of education on urban forests and urban trees in Europe. Presented here are the main findings of a comparative European study led by the Danish Forest and Landscape Research Institute within the framework of the EU-funded COST Action E12 'Urban Forests and Trees'. In order to obtain an overview of the status of higher education on urban forestry in Europe, 180 educational institutions in 28 countries were sent a questionnaire. Results show that urban forestry is an expanding element of European education, as urban elements are becom 相似文献
14.
Singsaas EL Laporte MM Shi JZ Monson RK Bowling DR Johnson K Lerdau M Jasentuliytana A Sharkey TD 《Tree physiology》1999,19(14):917-924
Because the rate of isoprene (2-methyl-1,3-butadiene) emission from plants is highly temperature-dependent, we investigated natural fluctuations in leaf temperature and effects of rapid temperature change on isoprene emission of red oak (Quercus rubra L.) leaves at the top of the canopy at Harvard Forest. Throughout the day, leaves often reached temperatures as much as 15 degrees C above air temperature. The highest temperatures were reached for only a few seconds at a time. We compared isoprene emission rates measured when leaf temperature was changed rapidly with those measured when temperature was changed slowly. In all cases, isoprene emission rate increased with increasing leaf temperature up to about 32 degrees C and then decreased with higher temperatures. The temperature at which isoprene emission rates began to decrease depended on how quickly measurements were made. Isoprene emission rates peaked at 32.5 degrees C when measured hourly, whereas rates peaked at 39 degrees C when measurements were made every four minutes. This behavior reflected the rapid increase in isoprene emission rate that occurred immediately after an increase in leaf temperature, and the subsequent decrease in isoprene emission rate when leaf temperature was held steady for longer than 20 minutes. We concluded that the observed temperature response of isoprene emission rate is a function of measurement protocol. Omitting this parameter from isoprene emission models will not affect simulated isoprene emission rates at mild temperatures, but can increase isoprene emission rates at high temperatures. 相似文献
15.
Hedie A. Bustamante Alfredo R. Rodríguez Daniel E. Herzberg Marianne P. Werner 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(4):405-411
Lameness is one of the most painful conditions that affects dairy cattle. This study was conducted to evaluate clinical signs and plasma concentration of several pain and stress biomarkers after oligofructose-induced lameness in dairy heifers. Lameness was induced using an oligofructose overload model in 12 non-pregnant heifers. Clinical parameters and blood samples were obtained at 48 and 24 h and at 6, 12, 24, 36 and 48 h after induction of lameness. Clinical parameters included heart rate, respiratory rate, ruminal frequency and lameness score. Plasma biomarkers included cortisol, haptoglobin, norepinephrine, beta-endorphin and substance P. Differences were observed in all parameters between control and treated heifers. The plasma concentration of biomarkers increased significantly in treated animals starting 6 h after induction of lameness, reaching maximum levels at 24 h for cortisol, 48 h for haptoglobin, 6 h for norepinephrine, 12 h for substance P and at 24 h for beta-endorphin. Overall, our results confirm that lameness associated pain induced using the oligofructose model induced changes in clinical parameters and plasma biomarkers of pain and stress in dairy heifers. 相似文献
16.
Intravenous sufentanil‐midazolam versus sevoflurane anaesthesia in medetomidine pre‐medicated Himalayan rabbits undergoing ovariohysterectomy 下载免费PDF全文
Patricia Hedenqvist Marianne Jensen‐Waern Åsa Fahlman Ragnvi Hagman Anna Edner 《Veterinary anaesthesia and analgesia》2015,42(4):377-385
ObjectiveTo compare physiological effects of sufentanil-midazolam with sevoflurane for surgical anaesthesia in medetomidine premedicated rabbits.Study designProspective, randomized controlled experimental study.AnimalsEighteen female Himalayan rabbits, weight 2.1 ± 0.1 kg.MethodsPremedication with 0.1 mg kg−1 medetomidine and 5 mg kg−1 carprofen subcutaneously, was followed by intravenous anaesthetic induction with sufentanil (2.3 μg mL−1) and midazolam (0.45 mg mL−1). After endotracheal intubation, anaesthesia was maintained with sufentanil-midazolam (n = 9) or sevoflurane (n = 9). Ovariohysterectomy was performed. Intermittent positive pressure ventilation was performed as required. Physiological variables were studied perioperatively. Group means of physiologic data were generated for different anaesthetic periods. Data were compared for changes from sedation, and between groups by anova. Post-operatively, 0.05 mg kg−1 buprenorphine was administered once and 5 mg kg−1 carprofen once daily for 2–3 days. Rabbits were examined and weighed daily until one week after surgery.ResultsSmooth induction of anaesthesia was achieved within 5 minutes. Sufentanil and midazolam doses were 0.5 μg kg−1 and 0.1 mg kg−1, during induction and 3.9 μg kg−1 hour−1 and 0.8 mg kg−1 hour−1 during surgery, respectively. End-tidal sevoflurane concentration was 2.1% during surgery. Assisted ventilation was required in nine rabbits receiving sufentanil-midazolam and four receiving sevoflurane. There were no differences between groups in physiologic data other than arterial carbon dioxide. In rabbits receiving sevoflurane, mean arterial pressure decreased pre-surgical intervention, heart rate increased 25% during and after surgery and body weight decreased 4% post-operatively. Post-operative problems sometimes resulted from catheterization of the ear artery.ConclusionSevoflurane and sufentanil-midazolam provided surgical anaesthesia of similar quality. Arterial blood pressure was sustained during sufentanil-midazolam anaesthesia and rabbits receiving sevoflurane lost body weight following ovariohysterectomy. Mechanical ventilation was required with both anaesthetic regimens.Clinical relevanceAnaesthesia with sufentanil-midazolam in medetomidine premedicated healthy rabbits is useful in the clinical and the research setting, as an alternative to sevoflurane. 相似文献
17.
Quantitative sandwich ELISA for the determination of tropomyosin from crustaceans in foods 总被引:3,自引:0,他引:3
The ubiquitous muscle protein tropomyosin has been identified as the major shrimp allergen and is suggested to be a cross-reacting allergen. Previously, only a few methods for the detection of tropomyosin in food have been published. A quantitative sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of tropomyosin from crustaceans in foods has been developed and validated. A polyclonal rabbit antitropomyosin capture antibody and the biotinylated conjugate of the same antibody for detection were the basis for the ELISA, which was specific for crustaceans. The ELISA was able to quantitate tropomyosin in various food matrixes, had a detection limit of 1 microg/g, and cross-reacted to some extent with cockroach. Recoveries ranged from 63 to 120%, and the intra and interassay coefficients of variation were <6 and <14%, respectively. 相似文献
18.
Marianne Frantzen Arne M. Arnesen Brge Damsgrd Helge Tveiten Helge K. Johnsen 《Aquaculture (Amsterdam, Netherlands)》2004,240(1-4):561-574
Four groups of Arctic charr (age 3+), previously exposed to natural photoperiod, were subjected to different photoperiod regimes from February 20: Simulated natural photoperiod (SNP; Tromsø 70°N), continuous light (LD 24:0) followed by short day (LD 6:18) from either May 1 (LFeb–May) or June 25 (LFeb–June), or continuous light (LD 24:0) throughout the experiment (LContinuous). In females, peak levels of estradiol-17β and testosterone were 6 and 10, 6 and 7, and 3 and 5 weeks advanced, in LFeb–May, LFeb–June and LContinuous, respectively, whereas in males plasma levels of 11-ketotestosterone peaked 8 weeks earlier in LFeb–May and LFeb–June than in SNP and LContinuous. Median ovulation time in LFeb–May and LFeb–June was advanced by 10 weeks compared to SNP and LContinuous, and median spermiation time (first observation of running milt in each individual) in LFeb–May, LFeb–June and LContinuous was advanced by 10, 7 and 5 weeks, compared to SNP. A switch from long to short day early in the reproductive cycle (LFeb–May and LFeb–June) apparently resulted in stronger synchronization of both ovulation and spermiation. Ovulation in LFeb–May and LFeb–June occurred over 3 weeks (LFeb–May) and 8 weeks (LFeb–June), as compared to 10 weeks in the SNP group, whereas all males (except one individual) began spermiating over a period of 3 weeks (LFeb–June), or less (LFeb–May), as compared to 7 weeks in the SNP group. Lack of a short day stimulus (LContinuous), on the other hand, resulted in larger variation in the timing of final maturation, with ovulation and spermiation being spread over 15 weeks. Low egg survival in LFeb–May (17% versus 60% and 97% for LContinuous and SNP) indicate that a long day signal of 10 weeks early in the reproductive cycle may be too short to ensure good egg and sperm quality. Photoperiod manipulation did not affect the proportion of maturing fish. 相似文献
19.
Fresh applications of phosphorus (P) may cause ‘incidental’ losses of dissolved and particulate P forms in land runoff when rainfall interacts directly with fertilizers and manures which are spread, or excreted, onto the soil surface. Research indicates that when incidental P losses (IPL) occur, they often make the dominant (50–98 %) contribution to measured P loads in surface and sub‐surface runoff from field plots, with increased risk of eutrophication where P‐enriched runoff is routed directly to the watercourse. Rates of P loss are temporally and spatially very variable (< 1 to 25 % of total P applied) depending on the amount of P applied; the P release properties of the materials applied (% P extractable in water), the timing of storm events after application and the amounts of runoff generated. Large P applications left on the surface of wet, frozen, compacted, and intensively underdrained soils in high rainfall areas are particularly vulnerable to IPL. Concentrations of P in runoff are often greatest during the first storm event following P application, but can remain high for several weeks, or even months after application. Catchment scale impacts are more difficult to quantify due to the effects of dilution with runoff from fields without IPL, and the contributions of P from other sources. Approaches to predicting IPL are discussed and need to be improved in order to assess the contribution of IPL to catchment P loads in river basin management planning. Strategies to control IPL should focus on adoption of more sensitive management practices in fields with a high risk of rapid runoff or preferential flow, and are likely to very effective. 相似文献
20.
We report a conceptual framework for a simple screening tool to evaluate the vulnerability of different fields in the landscape to phosphorus (P) loss within the European agricultural context. Our work builds on the principles established for the P Index system developed in the United States. We examine the differing needs and criteria for a range of EU member states and EFTA countries and highlight essential and optional criteria for inclusion in the decision support framework depending on their ecoregional characteristics. A framework such as this, meets in principle, the needs of the COST832 Action ‘Quantifying the Agricultural Contribution to Eutrophication’ as it deals with diffuse nutrient sources from agriculture, and aids more targeted use of practical strategies to mitigate nutrient losses to minimise the potential for the eutrophication of receiving waters. 相似文献