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91.
The pathogenicity of four isolates ofFusarium oxysporum obtained from infected cultivated rocket (Eruca vesicaria) and wild (sand) rocket (Diplotaxis tenuifolia) was tested on the following cruciferous hosts: stock, radish, wild and cultivated rockets, and various species in the cabbage
tribe: cabbage (Brassica oleracea var.sabauda), cauliflower (Brassica oleracea var.botrytis), Brussels sprouts (Brassica oleracea var.gemmifera), broccoli (Brassica oleracea var.italica), turnip (Brassica rapa var.rapa). The results indicated that isolates ofF. oxysporum from cultivated and wild rocket belong to theforma specialis raphani. The isolates from rocket were pathogenic on cabbage, Brussels sprouts, broccoli, turnip, radish and stock; isolates ofF. oxysporum conglutinans from cabbage and radish, and the isolate ofF. oxysporum f.sp.raphani from rape obtained from the ATCC collection, were pathogenic on both cultivated and wild rocket. 相似文献
92.
Florian Chain Gérard Riault Maxime Trottet Emmanuel Jacquot 《European journal of plant pathology / European Foundation for Plant Pathology》2007,117(1):35-43
Serial passage experiments (SPE) of a Barley yellow dwarf virus-PAV (BYDV-PAV) isolate were performed on Zhong ZH and TC14 wheat lines to evaluate the durability of their resistance to BYDV. At different passage numbers (from the 2nd to the 114th), biological properties of the produced isolates were recorded either by monitoring infection percentages and virus titers of the first 3 weeks of viral infection or by measuring their impact on yield components. Statistical analyses using the area under pathogen progress curves and the area under concentration progress curves demonstrated that these two resistant lines induce, after only a few passages, a selection of variant(s) with significantly modified infection abilities. Isolates resulting from SPE performed on these lines induced important decreases of yield components. These results indicate that the use of Zhong ZH and TC14 lines in BYDV-resistant breeding programmes should be approached with caution. 相似文献
93.
Laboratory rearing of insects for scientific research ensures a constant supply of insects. However, laboratory colonies may
diverge genetically and/or phenotypically from wild populations due to selection pressures in their artificial environment.
This study examined how variation in a colony ofTrichoplusia ni influenced reproducibility over time of bioassays of toosendanin (a limonoid extracted from the bark ofMelia azedarach). In four of five experiments insect growth was reduced significantly (P=0.05) by the presence of toosendanin in the diet. The fifth experiment showed the same trend, but the effect was not significant.
Mean larval mass of both control and treated insects also varied greatly among experiments. The considerable variability observed
in this population highlights the importance of replicating experiments in time, and not just space, to confirm reproducibility
of treatment effects.
http://www.phytoparasitica.org posting Jan. 23, 2007. 相似文献
94.
Land use changes operate at different scales. They trigger a cascade of effects that simultaneously modify the composition
or structure of the landscape and of the local vegetation. Mobil animals, and birds in particular, can respond quickly to
such multi-scalar changes. We took advantage of a long term study on the response of songbirds to land-use changes on four
Mediterranean islands in Corsica and Sardinia to explore the benefits of a multi-scale analysis of the relationships between
songbird distribution, vegetation structure and landscape dynamics. Field data and aerial photographs were used to describe
the vegetation at three different scales. Birds were censused by point counts. We used statistical variance decomposition
to study how bird distribution and vegetation at various scales were linked. We analysed multi-scale vegetation changes (floristic
composition, plot vegetation type, and landscape structure) and their consequences on bird distribution with multivariate
and non-parametrical tests. The distribution of most species was linked to at least two spatial scales. The weight of a given
scale was consistent with life-history traits for species whose biology was well-known. In the examples studied, vegetation
composition, vegetation type and landscape changes that resulted from land abandonment negatively affected birds depending
on open or heterogeneous areas. Our results emphasize that multi-scale analyses can greatly enhance our understanding of bird
distribution and of their changes. Management of these populations should take into account measures at various spatial scales
depending on the sensitivity of the species. 相似文献
95.
Heike Kappes Kurt Jordaens Frederik Hendrickx Jean-Pierre Maelfait Luc Lens Thierry Backeljau 《Landscape Ecology》2009,24(5):685-697
Habitat fragmentation is a major cause for species loss, but its effect on invertebrates with low active dispersal power,
like terrestrial gastropods, has rarely been studied. Such species can not cross a hostile habitat matrix, for which the predictions
of island theory, such as positive relations between species richness and patch size, should apply. In order to test this
prediction, we studied gastropod species diversity by assessing gastropod assemblage characteristics from 35 sites in 19 fragments
of deciduous old-growth forests in the Lower Rhine Embayment, Germany. Assemblages differed between larger (≥700 ha) and smaller
forests (<400 ha), those of large forests held a higher percentage of forest species. Although α-diversity was similar between
the two forest size classes, small forests often comprised matrix species, resulting in a higher β-diversity. Edge effects
on the species richness of matrix species were noticeable up to 250 m into the forest. Hierarchical partitioning revealed
that distance to disturbances (external edge, internal edges like roads) explained most assemblage variables, whereas forest
size and woodland cover within a 1 km radius from the sites explained only a few assemblage variables. Densities of two forest-associated
species, Discus rotundatus and Arion fuscus, decreased with forest size. Yet, forest size was positively correlated with richness of typical forest species and densities
of Limax cinereoniger. The latter species seems to need forests of >1,000 ha, i.e., well above the size of most fragments. In conclusion, the prediction
is valid only for forest species. The response to fragmentation is species specific and seems to depend on habitat specialization
and macroclimatic conditions.
Jean-Pierre Maelfait: Deceased. 相似文献
96.
Eckhard Koch Annegret Schmitt Dietrich Stephan Carola Kromphardt Marga Jahn Hermann-Josef Krauthausen Gustaf Forsberg Sigrid Werner Tahsein Amein Sandra A. I. Wright Federico Tinivella Maria L. Gullino Steven J. Roberts Jan van der Wolf Steven P. C. Groot 《European journal of plant pathology / European Foundation for Plant Pathology》2010,127(1):99-112
The current study was initiated to evaluate the efficacy of physical methods (hot water, aerated steam, electron treatment) and agents of natural origin (resistance inducers, plant derived products, micro-organisms) as seed treatments of carrots for control of Alternaria dauci and A. radicina. Control of both Alternaria species by seed treatment with the resistance inducers was generally poor. Results were also not satisfactory with most of the formulated commercial micro-organism preparations. Based on the average of five field trials, one of these, BA 2552 (Pseudomonas chlororaphis), provided a low but significant increase in plant stand. Among the experimental micro-organisms, the best results were obtained with Pseudomonas sp. strain MF 416 and Clonostachys rosea strain IK726. A similar level of efficacy was provided by seed treatment with an emulsion (1%) of thyme oil in water. Good and consistent control was generally achieved with the physical methods aerated steam, hot water and electron treatment. Aerated steam treatment was, apart from the thiram-containing chemical standard, the best single treatment, and its performance may at least partially be due to extensive pre-testing, resulting in dosages optimally adapted to the respective seed lot. In some of the experiments the effect of the hot water treatment, which was tested at a fixed, not specifically adapted dosage, was significantly improved when combined with a Pseudomonas sp. MF 416 or C. rosea IK726 treatment. The results are discussed in relation to the outcome of experiments in which the same seed treatment methods and agents were tested in other seed-borne vegetable pathosystems. 相似文献
97.
Christopher R. Thornton 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(3):347-353
Species of the genus Trichoderma are ubiquitous soil-borne fungi that exhibit antagonism towards a number of economically important plant-pathogenic fungi
and oomycetes. This review discusses recent developments in the use of monoclonal antibodies to detect these fungi in their
natural soil environments and to quantify their population dynamics during antagonistic interactions with saprotrophic competitors
in soil-based systems. Immunological approaches to detection and quantification are examined in relation to conventional plate
enrichment techniques and to nucleic acid-based procedures. An example of recent research using a mAb-based assay to quantify
the effects of saprotrophic competition on the growth of Trichoderma isolates in mixed species, soil-based, microcosms is presented. Future technological developments in immunoassays for tracking
Trichoderma populations in soil are discussed and results presented showing the accurate detection and visualization of a plant growth-promoting
isolate of T. hamatum in the rhizosphere of lettuce using mAb-based immunodiagnostic assays. 相似文献
98.
含PRRS病毒ORF5的伪狂犬病病毒TK基因缺失转移载体的构建 总被引:2,自引:0,他引:2
提取伪狂犬病病毒(PRV)BarthaK61株基因组DNA,用限制性内切酶KpnI充分消化,回收5.9kb片段(J片段),将其克隆于质粒pUC119 KpnI位点上,获得pBKJ。用两对针对PRV TK基因的特异性引物对重组质粒进行PCR鉴定,证明其中含有PRV TK基因。然后用KpnI、PstI和BamHI等限制性内切酶对其进行酶切分析,确定了克隆片段的物理图谱。进一步研究证实TK基因位于其中的 相似文献
99.
Cannas da Silva J Noordhuizen JP Vagneur M Bexiga R Gelfert CC Baumgartner W 《The Veterinary quarterly》2006,28(1):23-32
The nature of veterinary work in dairy health management in Europe has changed over the past years and will change even more dramatically in the near future. The consumers and the media show increasing concern about animal welfare, safety of products of animal origin and traceability of animal products. Farmers in Europe have to produce under strict, often expensive and laborious regulations, while still commercially competing with farmers outside the EU and not subject to the same rules. Veterinarians should adapt their knowledge and skills to the new challenges and developments of the dairy sector. Dairy farmers nowadays ask for support in areas that go beyond clinical activities: environmental protection, welfare, nutrition, grassland management, economics and business management. Bovine practitioners should be able to advise in many different areas and subjects--that is the challenge to our profession. Veterinary education with regards to cattle health management should start with individual animal clinical work, which constitutes the basis of herd health advisory programmes. The bovine practitioner should then look beyond that and regard the herd as the unit. Each diseased cow or group of cows should be detected early enough to avoid financial losses or such losses should be prevented altogether by detecting and managing risk factors contributing to disease occurrence. Herd health and production management programmes represent the first level to optimise dairy farm performance. Expansions to that should further be considered, comprising both animal health and welfare issues, as well as food safety and public health issues. The latter could be addressed by quality risk management programmes following the HACCP-principles. Cattle veterinarians should follow recent developments and invest in new skills and knowledge in order to maintain their usefulness to the modern dairy farmer. Finally we are convinced that the cattle practitioner should evolve into this direction, otherwise the veterinarian as we know him will miss the train in the next years. 相似文献
100.
Lopez-Jimena B Alonso Mdel C Thompson KD Adams A Infante C Castro D Borrego JJ Garcia-Rosado E 《Veterinary microbiology》2011,154(1-2):86-95
The distribution of viral genome in the tissues of juvenile European seabass (Dicentrarchus labrax) during the course of a Red Spotted Grouper Nervous Necrosis Virus (RGNNV) infection has not yet been described. The present study addresses this and indicates which target organs may be involved in viral replication. This information should enable more accurate detection of virus in asymptomatic carriers, and in turn help to control the spread of the disease. The aim of this study was to examine the pattern of expression of viral genomic segments RNA1 and RNA2, using two absolute real-time PCRs (RT-qPCR), over the course of a RGNNV infection after administering the virus by intramuscular injection. In situ hybridization was also used to locate the RNA2 viral segment in different organs throughout the infection. The experimental challenge provoked an acute form of viral nervous necrosis (VNN), with a resulting cumulative mortality of 37%. The RT-qPCRs designed allowed the detection of both genomic segments in all the organs tested (nervous and non-nervous tissues) at all sampling times examined. The highest viral RNA copy number was found in eyes, although viral replication appeared to begin in the brain. Viral replication was also recorded in pooled internal organs and in caudal fin. However, the increase in the viral RNA copy number in these organs did not result in an increased viral titre, which may indicate that a productive infection does not take place in non-nervous tissues, possibly due to a failure in a viral post-replication step. 相似文献