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151.
Catia Pereira Itziar Aurora Montalbán Olatz García-Mendiguren Tomás Goicoa Maria Dolores Ugarte Sandra Correia Jorge Manuel Canhoto Paloma Moncaleán 《Journal of Forest Research》2016,21(3):143-150
Pinus halepensis has been described as a drought-tolerant species with high plasticity to growth in different environments. Its eco-physiological characteristics could facilitate the use of this species in large afforestations in the future scenery of climate change. Somatic embryogenesis is a biotechnological tool with potential for large-scale clonal propagation. In order to establish an improved regeneration protocol for Pinus halepensis, the effects of different temperatures (18, 23, and 28 °C) and water availability conditions (2, 3, and 4 g L?1 Gelrite®), during initiation of embryonal masses on the rate of initiation, proliferation, maturation, and the number of embryos developed, were evaluated. It was found that environmental conditions during the initiation stage of Pinus halepensis somatic embryogenesis influence the success of initiation and proliferation. In contrast, there was no effect of these conditions on the maturation rates and the number of somatic embryos. Somatic embryos were obtained in all treatments tested, indicating that plants can be produced from extreme conditions of induction, such as high temperatures (28 °C) and low water availability conditions (4 g L?1). 相似文献
152.
153.
Jorge Miguel Silva Faria Inês Sena Bruno Ribeiro Ana Margarida Rodrigues Carla Maria Nobre Maleita Isabel Abrantes Richard Bennett Manuel Mota Ana Cristina da Silva Figueiredo 《Journal of pest science》2016,89(1):207-217
The Columbia root-knot nematode (CRKN), Meloidogyne chitwoodi, is an EPPO A2 type quarantine pest since 1998. This nematode causes severe damage in economically important crops such as potato and tomato, making agricultural products unacceptable for the fresh market and food processing. Commonly used nematicidal synthetic chemicals are often environmentally unsafe. Essential oils (EOs) may constitute safer alternatives against RKN. EOs, isolated from 56 plant samples, were tested against CRKN hatching, in direct contact bioassays. Some of the most successful EOs were fractionated and the hydrocarbon molecules (HM) and oxygen-containing molecules (OCM) fractions tested separately. 24 EOs displayed very strong hatching inhibitions (≥90 %) at 2 µL mL?1 and were further tested at lower concentrations. Dysphaniaambrosioides, Filipendula ulmaria, Ruta graveolens, Satureja montana and Thymbra capitata EOs revealed the lowest EC50 values (<0.15 µL mL?1). The main compounds of these EOs, namely 2-undecanone, ascaridol, carvacrol, isoascaridol, methyl salicylate, p-cymene and/or γ-terpinene, were putatively considered responsible for CRKN hatching inhibition. S. montana and T. capitata OCM fractions showed hatching inhibitions higher than HM fractions. The comparison of EO and corresponding fractions EC50 values suggests interactions between OCM and HM fractions against CRKN hatching. These species EOs showed to be potential environmentally friendly CRKN hatching inhibitors; nonetheless, bioactivity should be considered globally, since its HM and OCM fractions may contribute, diversely, to the full anti-hatching activity. 相似文献
154.
Rauter AP Filipe MM Prata C Noronha JP Sampayo MA Justino J Bermejo J 《Fitoterapia》2005,76(5):433-438
Diacronema sp. was cultured and its sterols were separated by column chromatography on silica gel. The new sterol 24-ethyl-4alpha-methyl-cholestane-3,20-diol (1) was characterised by NMR and MS spectrometry, as well as (22E)-24-ethyl-4alpha-methyl-5alpha-cholest-22-en-3beta-ol (2) and beta-sitosterol, the major components of the sterol fractions. Neither the biosynthetic origin of the new dihydroxysterol nor its role in the biochemistry of Diacronema is known. 相似文献
155.
A new acylated quercetin glycoside, quercetin 3-O-(2-t-p-coumaroyl)-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranoside (1), together with the known 3-(3,4-dihydroxyphenyl)lactic acid (2) were isolated from Ranunculus lanuginosus leaves and identified on the basis of detailed spectral analysis, including 2D-NMR spectrometry and ESI-MS. 相似文献
156.
Maria Alcubilla Hertha v. Aufsess R. Heibl K. E. Rehfuess 《Forstwissenschaftliches Centralblatt》1988,107(1):205-218
In einem kombinierten Düngungs- und Infektionsversuch wurden die Beziehungen zwischen dem Ern?hrungszustand, dem Wachstum
und der Rotf?uledisposition von N-Mangel-Fichten auf Kalkmergel-Rendzina geprüft. Die vorliegende Arbeit berichtet über die
Wirkung der N-Düngung und künstlicher Infektionen mit Heterobasidion-Mycel auf den Gehalt an Extraktstoffen im Bast und Holz.
Wurzelbast vom August (nicht aber vom April) von mehrmals gedüngten Fichten enthielt mehr Chloroform-und Ethanol/Wasser-Extrakt
sowie Zucker- und Phenolkomponenten als die ungedüngten Varianten. Auch das Wurzelholz gedüngter Fichten war im August reicher
an Extraktstoffen. Die N-Gaben steigerten au?erdem in beiden Geweben den Gehalt an l?slichen N-Verbindungen. Im Stamm erh?hte
die Düngung die Gehalte an. Methanol-Extrakt sowie an einigen N-haltigen und phenolischen Verbindungen nur im ?u?eren Splint. 相似文献
157.
Variations in soil chemical properties and microbial biomass in artificial gaps in silver fir stands
Changes in soil chemical and microbiological parameters in small (185 m2) and medium (410 m2) gaps, created in mature silver fir (Abies alba Mill) stands (mean height 30 m) in the Calabrian Apennines (Southern Italy), were studied over 2 years. Medium gaps had high
soil temperature and photosynthetically active radiation (PAR) transmittance and low soil moisture. Within small gaps we detected
a greater amount of organic matter, with respect to under canopy cover sites and medium gaps. Moreover, a different trend
of the organic matter between small and medium gaps was observed. In the medium gaps, with respect to under canopy cover sites
and small gaps, we found a relatively low content of organic matter, associated to a lower amount of humic acid, which suggested
that the organic substrate undergoes a mineralization rather than a humification process. An opposite trend was observed in
small gaps, where the increase of organic matter content, associated to an increase of humic acid and microbial biomass, suggested
a better humification process. Moreover, in small gaps the highest level of urease and the greatest fluorescein diacetate
(FDA) hydrolysis were observed. The different trend of organic matter observed in small and medium gaps may be related to
changes in the environmental conditions. PAR transmittance, significantly higher in medium gaps, contributed to increase soil
temperature and decrease soil moisture, affecting soil microbial populations and organic matter trend. These results suggest
that the creation of small gaps represents the silvicultural practice with minor environmental impact. 相似文献
158.
159.
Maria Alcubilla Hertha v. Aufsess R. Heibl K. E. Rehfuess 《European Journal of Forest Research》1988,107(1):205-218
Zusammenfassung In einem kombinierten Düngungs- und Infektionsversuch wurden die Beziehungen zwischen dem Ern?hrungszustand, dem Wachstum
und der Rotf?uledisposition von N-Mangel-Fichten auf Kalkmergel-Rendzina geprüft. Die vorliegende Arbeit berichtet über die
Wirkung der N-Düngung und künstlicher Infektionen mit Heterobasidion-Mycel auf den Gehalt an Extraktstoffen im Bast und Holz.
Wurzelbast vom August (nicht aber vom April) von mehrmals gedüngten Fichten enthielt mehr Chloroform-und Ethanol/Wasser-Extrakt
sowie Zucker- und Phenolkomponenten als die ungedüngten Varianten. Auch das Wurzelholz gedüngter Fichten war im August reicher
an Extraktstoffen. Die N-Gaben steigerten au?erdem in beiden Geweben den Gehalt an l?slichen N-Verbindungen. Im Stamm erh?hte
die Düngung die Gehalte an. Methanol-Extrakt sowie an einigen N-haltigen und phenolischen Verbindungen nur im ?u?eren Splint.
Die Inokulation der St?mmchen durch Anbohren induzierte die Bildung von Reifholz und übergangszonen (zwischen Splint und verf?rbtem
Reifholz), die besonders reich an Petrolether-Extrakt, Methanol-Extrakt und Lignanen waren. Diese Reaktionen der Fichten waren
durch Düngung nicht beeinflu?t, da sie in inneren, vor der Düngung angelegten Stammbereichen abliefen.
Die wiederholte N-Düngung führte im Wurzelbast vom April zu geringerer, in den August-Proben zu gesteigerter Hemmwirkung gegenüber
Heterobasidion annosum im Biotest. Die Hemmung im August war mit dem Gehalt an phenolischen Komponenten positiv korreliert.
Wurzelholz und ?u?erer Stamm-Splint von gedüngten B?umchen enthielten mehr Stickstoff und mehr Aminos?uren als die Kontrollfichten
und stellten deshalb ein besonders günstiges, Substrat für den Pilz dar. Die Hemmwirkung des Reifholzes war positiv korreliert
mit dem Gehalt an Methanol-Extrakt bzw. an Lignanen; ein Düngungseinflu? war nicht erkennbar. Für Stammholzfraktionen der
inokulierten Fichten fand sich ein positiver Zusammenhang zwischen der Hemmwirkung und dem Gehalt an harzartigen Komponenten,
die als Reaktion des Baumes auf die Verletzung vermehrt produziert wurden.
Nitrogen fertilization experiments in a Norway spruce stand (Picea abies Karst.) of stagnant growth on devastated marly rendzina: III. Influence of fertilization and inoculation on the extractives in phloem and wood
Summary A combined fertilization and inoculation experiment was designed to study the interrelationships between nutritional status, growth, and susceptibility to heart rot of planted N-deficient Norway spruce growing on a rendzina soil (Rendoll). The present report describes the impact of N-fertilization and of inoculation withHeterobasidion annosum mycelium on the contents of extractives in the phloem and in the wood. Root phloem in August (but not in April) of spruces fertilized repeatedly contained more chloroform- and ethanol/water-soluble substances, and more sugars and phenolic compounds as compared with phloem from the controls. Root wood of fertilized trees was also richer in extractives. N-fertilization, in addition, always raised the contents of soluble N-fractions in both tissues, as well as the amount of methanol-soluble substances, N-containing compounds, and phenolics in the outer sapwood region of the stem. Inoculation of stems by introducingH.a. mycelium into auger holes induced the formation of heartwood and of transition zones (between sapwood and heartwood) which were especially rich in petrolether- and methanol-soluble substances and in lignans. These reactions were not influenced by fertilization, since they were located in the inner stem regions formed in advance of fertilization. Repeated N-application reduced the fungistatic effect onH.a. of root phloem in April but increased it in August. The inhibition in August was positively correlated with the contents of phenolic compounds. Root wood and outerstem sapwood of fertilized spruces were richer in N and amino acids than control trees and therefore offered a favorable substratum for the fungus. The inhibitory effect of heartwood was positively related to the content of methanol-soluble compounds and lignans; an effect of fertilization could not be found. For wood fractions of inoculated trees a positive interrelationship was detected between fungistatic effect and content of resin compounds, the production of which was promoted as a reaction of the tree to wounding.相似文献
160.
Guarrera PM 《Fitoterapia》2005,76(1):1-25
In this study, the more significant results of extensive ethnopharmacobotanical research carried out by the author in the years 1977-2000 in 175 localities of three regions of Central Italy (Marche, Abruzzo, and Latium) have been reported and compared. The usages of 80 species belonging to 36 families are described, of which 71 were used in human therapy and 29 in veterinary medicine. Uses are suited with the number of localities in which they have been mentioned. Among the wild plant mainly still used, Malva sylvestris, Urtica dioica, and Sambucus nigra are particularly highly considered, while major uses of plants concern these plants in addition to Allium sativum, Rubus ulmifolius, Parietaria diffusa, Cynodon dactylon, and Ficus carica. Unusual phytotherapic uses concern Brassica oleracea, Taraxacum officinale (warts), Ruscus aculeatus, S. nigra (chilblains), Allium cepa (chilblains; to remove thorns and splinters), Juglans regia, R. ulmifolius (burns), and Euphorbia paralias (bites of weevers). Among new uses with only one quotation, we remember Cirsium arvense (intestinal disturbances), Centaurea bracteata (cough), Lupinus albus (calluses), Melittis melissophyllum (eye inflammations, antispasmodic), and Artemisia absinthium (tendon inflammations), while among plants employed in various regions with interesting less-known properties, there are C. arvense (emergency haemostatic), P. diffusa (insect bites), and Scrophularia canina (antiseptic and cicatrizing agent for wounds in bovines and sheep). 相似文献