辽宁绒山羊CSN3基因PCR-RFLP多态性分析

本试验以辽宁绒山羊和内蒙古绒山羊为实验动物,采用PCR-RFLP技术对-酪蛋白基因(CSN3)片段的Ⅰ的酶切多态性进行分析,结果表明:辽宁绒山羊和内蒙古绒山羊2个山羊群体中均存在CSN3基因Ⅰ酶切位点的多态性,且均有A、B两个等位基因。辽宁绒山羊群体等位基因A和B的基因频率分别为0.46和0.54;内蒙古绒山羊群体中等位基因A和B的基因频率分别为0.31和0.69。CSN3基因Ⅰ酶切位点的基因型分布在辽宁绒山羊群体中极显著偏离Hardy-Weinberg平衡定理(<0.01),在内蒙古绒山羊群体中符合Hardy-Weinberg平衡定理(>0.05),但两个群体间存在显著差异(<0.01)。     

Uterine Expression of WNT7A and β-catenin After Induction of Oestrus in Sheep

WNT7A and β-catenin localisations and roles in regulating periimplantation ovine conceptus development under natural estrous conditions have been elaborated.However,their locations and expression patterns have not been reported under induction of oestrus.The localisation,expression and function of WNT7A and β-catenin in the uterine tissues of the early pregnant and non-pregnant sheep on days 10,12,14,16 and 18 following artificial induction of oestrus were investigated by means of in situ hybridisation,real-time RT-PCR,immuno-histochemistry and western blotting methods.WNT7A and β-catenin mRNA and protein were both restricted to the apical surfaces of the uterine luminal epithelium (LE) and glandular epithelium (GE).In pregnant sheep,protein localisation of WNT7A and β-catenin was observed both in the endometrial LE and GE.Their staining presented on day 10,increased between day 12 and day 16,and decreased on day 18.WNT7A and β-catenin mRNA and protein expression increased initially and then decreased from day 10 to day 18,peaking on day 16,and β-catenin reaching a peak on day 18 in the uterine tissues of pregnant sheep (p0.05).By contrast,no significant changes in WNT7A and β-catenin mRNA and protein expression levels were observed from day 10 to day 18 of the oestrus cycle in the uterine tissues of non-pregnant sheep (p0.05).Additionally,WNT7A and β-catenin mRNA and protein expression levels in the uterine tissues of the early pregnant sheep were significantly higher than those of non-pregnant sheep (p0.05).Treatment of endometrial epithelial cells with WNT7A increased the mRNA expressions of β-catenin,c-myc and Cyclin D1.These results provided an underlying mechanism of periimplantation ovine conceptus development under induction of oestrus.     

景观园林施工设计及绿化养护技术要点分析

无论是景观园林施工还是绿化养护,均是城市环境建设的重要组成部分,与人们的生产生活 息息相关。大多数综合性景观园林施工面临成本难以控制的问题,因其工程量较大、施工周期较长、 周边环境复杂,整个施工过程不可预测的变数较多,施工成本和施工难度居高不下。文章主要就景观 园林施工设计及绿化养护技术要点进行了分析与阐述,意在提高景观园林施工及绿化养护的水平和 质量。     

不同控释氮肥运筹对粳稻养分吸收与氮素利用的影响

以绥粳18为供试材料,通过盆栽试验,在一次性施入控释氮肥免追肥条件下,设置不同施肥量(高、中、低、无)和施肥深度(5 cm、10 cm),探讨不同氮肥施用方式对粳稻养分吸收和氮素利用的影响。结果表明,在不同氮肥运筹条件下,粳稻各生育期氮、磷、钾的吸收、转运均存在明显的协同效应,施肥深度5 cm组,随着氮肥施用量的增加,粳稻的氮吸收总量增加,同时可促进磷的吸收,但影响幅度较小。钾变化与氮、磷变化有所不同。施肥深度10cm组,施高量氮肥抑制了粳稻对磷的吸收、减少了对氮的吸收,但有利于提高氮素利用率,对钾的吸收无显著影响。施用树脂包膜控释肥,纯氮用量为153 kg/hm^2、施肥深度5 cm、一次性施肥免追肥可获得最优水稻氮素利用效率并显著提高磷钾肥利用效率。     

An integrated rice panicle phenotyping method based on X-ray and RGB scanning and deep learning

Rice panicle phenotyping is required in rice breeding for high yield and grain quality.To fully evaluate spikelet and kernel traits without threshing and hulling,using X-ray and RGB scanning,we developed an integrated rice panicle phenotyping system and a corresponding image analysis pipeline.We compared five methods of counting spikelets and found that Faster R-CNN achieved high accuracy(R~2 of 0.99)and speed.Faster R-CNN was also applied to indica and japonica classification and achieved 91%accuracy.The proposed integrated panicle phenotyping method offers benefit for rice functional genetics and breeding.     

菊花幼嫩花瓣愈伤组织的诱导和植株再生

以菊花幼嫩花瓣作为供体材料,ＭＳ作为基本培养基,在激素组合为６ＢＡ１～３ｍｇ／Ｌ＋ＮＡＡ１ｍｇ／Ｌ或２,４ Ｄ１～３ｍｇ／Ｌ＋ＫＴ１ｍｇ／Ｌ的范围内均能诱导出愈伤组织,但以ＭＳ附加６ＢＡ２ｍｇ／Ｌ和ＮＡＡ１ｍｇ／Ｌ的诱导率最高,可达１００％。将愈伤组织转移到ＭＳ＋６ＢＡ２ｍｇ／Ｌ＋ＮＡＡ０２ｍｇ／Ｌ的培养基上,分化芽的频率为７７８％。将３ｃｍ左右的茎段转移到不含激素的ＭＳ培养基或ＭＳ＋ＮＡＡ０１ｍｇ／Ｌ的培养基上,生根率可达９５％以上。     

人参生长过程中根重变化规律的研究

通过田间试验,对1993-2001年人参生长过程中根重变化规律进行了研究,并采用数学模拟的方法。对参根增重过程中某些数量规律和特点进行了分析。结果表明：参根增重的变化过程为一个S形曲线。曲线特征为逻辑斯谛(Logistie)增长曲线;参根增重速度(P)是一个连续变化的单峰曲线,极值点出现在第5年,年最大增重速度为22．65g;突变点出现在t1=3．5(年)。t2=6(年),在t1之前和t2之后,根重增长缓慢,在t1-t2之间,根重增长迅速。     

基于响应面法的杨树桑黄菌种液体培养基优化

杨树桑黄(Sanghuangporus vaninii)作为大型药用真菌,由于其生长年限长且开发不合理,导致其资源短缺,而通过液体发酵培养可以在短时间内得到大量菌丝体.本试验以菌丝体生物量为主要指标,对杨树桑黄液体培养基进行优化.通过响应面分析法对杨树桑黄液体培养基的碳源、氮源、无机盐进行筛选.结果表明:杨树桑黄ST菌...     

基于中小型制造业的MCR控制、实施、考核研究——以美国卡莱轮胎公司为例

不合理的生产计划可能导致生产出客户不需要的产品而产生大量库存,或者是订单充足但因计划安排不合理造成拖欠订单等问题,进而导致生产计划和生产现场之间的信息脱节.针对传统生产计划中存在的这些问题,提出了以生产时间、产量和主生产计划等为约束条件,采用MCR(Mixing Completion Rate,混合完成率)控制表对生产计划进行控制.实践表明,将此控制工具应用于卡莱轮胎公司的生产中,成功地降低了企业库存,加速了企业资金流动,充分表明了该控制工具在企业生产中具有良好的应用性.     

利用RAPD技术对牡丹江地区红松根际土壤微生物多样性的分析

土壤微生物对土壤中有机质的转换起着至关重要的作用,土壤微生物多样性的变化对地表生态系统、土壤的结构、土壤的肥力和植物的健康等有重要的影响.本文利用RAPD技术分析牡丹江地区的3个林型及其3个不同林龄共9块样地的根际微生物的多样性,得到了椴树红松林、蒙古栎红松林和云冷杉红松林3种林型内的土壤微生物多样性的变化趋势,及其微生物构成差异性.