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31.
32.
Almundena Bayo-Canha Luciana Delgado-Benarroch Julia Weiss Marcos Egea-Cortines 《Scientia Horticulturae》2007
We have studied the effect of artificial modification of leaf area on inflorescence and floral quality in Antirrhinum majus. We used two treatments where we clipped all the leaves except the first three pairs of true leaves, or we cut every leaf visible by the time the plant had six leaf pairs. Decreased leaf area caused a decrease in stem elongation that could be traced back to a decrease in internode elongation. Leaves left on the plant increased their size beyond control. Inflorescence quality was strongly affected since total floral number showed a strong decrease with reduced leaf area. In contrast, floral size hardly changed. Breeding programs in Antirrhinum should take into account growth conditions affecting leaf area when searching for changes in inflorescence quality while floral size can be screened independently of this factor. 相似文献
33.
Plant breeders have been trying to predict the performance of hybrids based on their parental performance. One application of molecular markers is its use in selection. The objectives were to map quantitative trait loci (QTL) and verify its congruence in maize lines and in their testcrosses and verify the possibility to select testcrosses from the predicted means of the lines by using information from markers. Two-hundred and fifty six lines and the testcrosses of these lines with two testers were evaluated in six environments, considering grain yield, plant lodging, days to anthesis and silking, anthesis-silking interval, plant and ear height and ear placement. QTL were mapped in the lines and in testcrosses and the predicted means of the lines were computed based on QTL effects and in all markers of the genome. The congruence of QTL detected in the lines and testcrosses were small for all traits. The correlations between the predicted means of the lines and the phenotypic means of the testcrosses ranged from low for grain yield to moderate for cycle and stature traits. The highest coincidences of the lines and selected testcrosses were observed for cycle and stature traits and the lowest for grain yield. Even by using molecular markers information, it is only possible to predict the testcrosses performance from the lines information to less complex traits and with reduced dominance effect. For complex traits and with pronounced dominance effect, information of markers must be obtained directly in the testcrosses, so they can be used for selection. 相似文献
34.
Electro-acupuncture and Chinese herbs for treatment of cervical intervertebral disk disease in a dog
Ayne Murata Hayashi Júlia Maria Matera Tatiana Soares da Silva Ana Carolina Brand?o de Campos Fonseca Pinto Sílvia Renata Gaido Cortopassi 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):95-98
A non-ambulatory dog with tetraparesis following a pain episode that had evolved over 2 months was submitted for medical treatment and diagnosed with intervertebral disk disease at C3-C4 and dorsal extradural compression at C1-C2 and C3-C4 using myelography and computed tomography. The dog experienced ambulation recovery after 15 days of treatment with only electroacupuncture and Chinese herbal medicine, with marked improvement occurring after only 10 treatments. Six months of follow-up demonstrated that the dog was stable and had no recurrence of symptoms. Therefore, it was concluded that the combination of electroacupuncture and Chinese herbal medicine was responsible for motor rehabilitation. 相似文献
35.
Soares RM Lopes EG Keid LB Sercundes MK Martins J Richtzenhain LJ 《Veterinary parasitology》2011,175(1-2):168-172
Toxoplasma gondii, Hammondia hammondi, Neospora caninum, Neospora hughesi and Hammondia heydorni are members of the Toxoplasmatinae sub-family. They are closely related coccidians with similarly sized oocysts. Molecular diagnostic techniques, especially those based on polymerase chain reaction (PCR), can be successfully applied for the differentiation of Hammondia-like oocysts. In this paper, we describe a rapid and simple method for the identification of H. heydorni oocysts among other members of the Toxoplasmatinae sub-family, using a heminested-PCR (hnPCR-AP10) based on a H. heydorni RAPD fragment available in molecular database. DNA of oocysts of H. heydorni yielded a specific fragment of 289-290 bp in the heminested-PCR assay. No product was yielded when the primers were used for the amplification of DNA extracted from T. gondii, N. caninum, N. hughesi and H. hammondi, thus allowing the differentiation of H. heydorni among other members of the Toxoplasmatinae sub-family. The hnPCR-AP10 was capable of detecting H. heydorni genetic sequences from suspensions with at least 10 oocysts. In conclusion, the hnPCR-AP10 proved to be a reliable method to be used in the identification of H. heydorni oocysts from feces of dogs. 相似文献
36.
Carlos E. Fidelis Manoela Franke Letícia C. R. de Abreu Tomasz Jagielski Mrcio G. Ribeiro Marcos V. dos Santos Juliano L. Gonalves 《Journal of veterinary diagnostic investigation》2021,33(6):1168
We evaluated the use of MALDI-TOF MS for the identification of 3 major, dairy-associated Prototheca species, namely, Prototheca bovis (formerly P. zopfii genotype 2), P. blaschkeae, and P. ciferrii (formerly P. zopfii genotype 1). The MALDI-TOF MS spectra established for those species were introduced into the reference spectra library of the Bruker Biotyper MALDI-TOF MS analysis software. Next, 31 Prototheca isolates from Holstein cows with mastitis, from herds located in the midwestern area of São Paulo State, Brazil, were subjected to MALDI-TOF MS profiling. MALDI-TOF MS allowed identification of 22 of 27 P. bovis and 3 of 4 P. blaschkeae isolates with scores >2.0, with 5 of 27 P. bovis and 1 of 4 P. blaschkeae isolates identified only to the genus level. With our extended algae database, MALDI-TOF MS can contribute to quick and effective speciation of Prototheca from mastitis cases. 相似文献
37.
The recent change in the regional development strategy of the European Union (EU) results in an important need to study the terms of change and their respective implications. This study seeks, through a bibliometric analysis, to ascertain the developments taking place in studies on research and innovation strategies for smart specialisation (RIS3) to identify shortcomings and opportunities for future research. This bibliometric review drew upon the Scopus database with the sample selected containing all the articles containing the keywords “Research and Innovation Strategies for Smart Specialisation” or “RIS3.” This correspondingly reports how some authors maintain that the path the EU should take involves imitative innovation. Meanwhile, others propose that each region should specialise in those industries that are already established there and thus avoid targeting any areas they do not already know/specialise in. Our findings detail six clusters in RIS3 research, which help in contextualising the literature review: (a) business discovery; (b) smart specialisation; (c) innovation; (d) specialisation; (e) regional policies; and (f) regional development. This study furthermore sets out perspectives for future lines of research and correspondingly seeks to convey a vast theoretical basis that may serve as the point of departure for future studies. 相似文献
38.
AISB Villaverde EG Fioratti FC Landim-Alvarenga JC de Carvalho Balieiro CM Melo FO Papa MD Lopes 《Reproduction in domestic animals》2009,44(S2):309-312
This study was aimed at assessing the changes in sperm motion patterns and the percentage of acrosome reaction (AR) in domestic cat semen after treatment with either ionomycin or progesterone (P4 ). Ten ejaculates were collected from five tomcats using an artificial vagina, and were diluted, centrifuged and resuspended in a capacitation medium. Samples were evaluated and divided into seven equal aliquots and, after 2 h at 25°C, were incubated for 30 min at 38°C in 5% CO2 and then analyzed. Computer-assisted sperm analysis and a combination of three fluorescent probes were used to assess sperm plasma, acrosomal membrane integrity and mitochondrial transmembrane potential. Thirty minutes after the start of incubation, P4 was added (10 μg/ml) to the P1 group. Groups P2 and P3 were supplemented with P4 (10 and 20 μg/ml, respectively) only after 2 h of incubation, and groups I1 and I2 were supplemented with ionomycin (4 and 8 μ m , respectively) 2 h after incubation. Group E was supplemented with ethanol (0.6%) at 2 h after incubation and group C received no supplementation. Ionomycin and P4 treatments led to a hyperactivation-like sperm motion and an increase (p < 0.05) in the percentage of AR. Although a higher (p < 0.05) percentage of AR was obtained in group I2 when compared with all P4 groups, a decrease (p < 0.05) in total and progressive motility was observed in I2 group. As I1 group was similar to I2 to induce AR without diminishing sperm motility, we can conclude that ionomycin at 4 μ m seems to be more suitable to trigger AR in domestic cat sperm. 相似文献
39.
Laura de Andrade Moura Ana Carolina Marqui de Almeida Thaisa Francielle Souza Domingos Fredy Ortiz-Ramirez Diana Negr?o Cavalcanti Valéria Laneuville Teixeira André Lopes Fuly 《Marine drugs》2014,12(5):2471-2484
Cardiovascular diseases represent a major cause of disability and death worldwide. Therapeutics are available, but they often have unsatisfactory results and may produce side effects. Alternative treatments based on the use of natural products have been extensively investigated, because of their low toxicity and side effects. Marine organisms are prime candidates for such products, as they are sources of numerous and complex substances with ecological and pharmacological effects. In this work, we investigated, through in vitro experiments, the effects of three diterpenes (pachydictyol A, isopachydictyol A and dichotomanol) from the Brazilian marine alga, Dictyota menstrualis, on platelet aggregation and plasma coagulation. Results showed that dichotomanol inhibited ADP- or collagen-induced aggregation of platelet-rich plasma (PRP), but failed to inhibit washed platelets (WP). In contrast, pachydictyol A and isopachydictyol A failed to inhibit the aggregation of PRP, but inhibited WP aggregation induced by collagen or thrombin. These diterpenes also inhibited coagulation analyzed by the prothrombin time and activated partial thromboplastin time and on commercial fibrinogen. Moreover, diterpenes inhibited the catalytic activity of thrombin. Theoretical studies using the Osiris Property Explorer software showed that diterpenes have low theoretical toxicity profiles and a drug-score similar to commercial anticoagulant drugs. In conclusion, these diterpenes are promising candidates for use in anticoagulant therapy, and this study also highlights the biotechnological potential of oceans and the importance of bioprospecting to develop medicines. 相似文献
40.
Jos Diomedes Barbosa Carlos Magno C. de Oliveira Marcos Dutra Duarte Gabriela Riet-Correa Paulo Vargas Peixoto Carlos Hubinger Tokarnia 《Journal of Equine Veterinary Science》2006,26(9):393-398
The clinical and pathological aspects of a neurological disease observed in 16 horses in Pará, Amazonia, Brazil, are presented. The symptoms were mainly motor incoordination, paresis of the tongue, somnolence, difficulties in apprehension, chewing and swallowing of food, as well as instability and standing with abducted members. The clinical course was subacute or chronic and in most cases was not fatal. Postmortem examination performed in one already very sick, euthanized animal, did not show significant macroscopic lesions; histopathological examination revealed slight edema and degenerative alterations of a few axons, mainly in the medulla oblongata. In all pastures where horses were affected, plenty of bamboo had been eaten, probably because of scarcity of pasture. By feeding large amounts of fresh bamboo leaves of this region, in different growing stages, to three horses (horse 1, 47 g/kg/d for 30 days; horse 2, 10 g/kg/d for 60 days; horse 3, 18 g/kg on the first day, and 31 g/kg/d for 6 more days)—the animals ate the leaves unassisted—it was possible to reproduce nervous symptoms essentially identical to those observed in the natural disease 24 to 72 hours after the first feeding of the plant. In spite of continuous administration of the plant, intensity of the clinical signs did not increase. Based on field observations and comparison of the clinical and pathological pictures seen in the natural and experimental disease, the described illness can be concluded to be caused by the ingestion of large amounts of the leaves of Bambusa vulgaris f. vulgaris. 相似文献