Adaptation of the tropical hybrid Euphorbia × lomi Rauh to the exposure to the Mediterranean temperature extremes

Poysean (Euphorbia × lomi Rauh) has been introduced in the Mediterranean countries as an ornamental plant for indoor uses. When used outdoor, few information is available about its ability to withstand the Mediterranean temperature extremes. An experiment in an open stand was performed to evaluate poysean tolerance to the winter and summer temperatures of the thermo Mediterranean climate and its ornamental value for urban greening. Two genotypes of poysean (Nguen Muang, NM; and Soi Budsanin, SB) with similar bracts and flower colours were grown at either 2 or 3 plants per pot during 23 months. Number of flower racemes and leaves per plant and plant height were recorded monthly. Overall ornamental value of each pot was scored by means of a panel test (PT) performed by independent examiners. Score assignment was homogeneous between examiners. On average, NM showed a 36% lower number of flower racemes, but a 30% higher ornamental value than SB. During fall and beginning of the winter, NM showed a higher number of flower racemes per plant than SB. Increasing plant density from 2 to 3 plants per pot did not influence flower racemes and leaves per plant. From March to November of the second year, number of flower racemes in NM was similar to the first year, whereas SB flower production was markedly higher, which can be due to a genotypic difference to Mediterranean temperature tolerance. Correlation between number of flower racemes per pot and ornamental value was higher in SB than NM, which suggest that in NM flower abundance contribute less to the pot ornamental value. The present data suggest that poysean is able to withstand the Mediterranean temperature extremes and still produces flower racemes during winter, which indicate it as a suitable ornamental plant in outdoor applications.     

Time trend in reference evapotranspiration: analysis of a long series of agrometeorological measurements in Southern Italy

This study aims to evaluate the potential effects of the climatic variations on the reference evapotranspiration (ET₀) and, consequently, on the crop water requirements in the Apulian Tavoliere, one of the largest irrigated districts of Southern Italy. To reach this purpose, both climatic parameters (air temperature and rainfall) and estimated water requirements of ??processing?? tomato (among the most representative irrigated crops in the district since the mid-1970s and, therefore, chosen as a study-case) were analyzed in order to find out if a time trend exists or does not. The analysis covered the period from 1957 to 2008. The analysis showed that the rainfall amounts decreased (?3.4 mm per year in the analyzed period), while air temperature increased (0.18 °C and 0.25?°C per decade for minimum and maximum, respectively). As a consequence of the climatic variation during the considered period, a growth trend of the ET₀ (1.4 mm per year) and water deficit (3.2 mm per year) took place. As a consequence, the water amounts for irrigating the same crop in the considered period were growing. This increased consumption is in agreement with the perception of the farmers of the district but never documented. Through the FAO AquaCrop model, the tomato irrigation water requirements have been simulated during the considered period. The trend analysis of the seasonal evapotranspiration values simulated in 52 years confirmed the increase in tomato water requirements (0.7 mm per year).     

Modelling the event-based hydrological response of olive groves on steep slopes and clayey soils under mulching and tillage management using the SCS-CN,Horton and USLE-family models

The SCS-CN, Horton and USLE-family models are used worldwide, but few examples are available for olive groves on steep and clayey soils under mulching of pruning residues. We evaluate the accuracy in predicting runoff and soil loss of a steep (20%) and clayey olive grove subject to three soil conservation practices: mechanical tillage (MT), mulching with pruning residues (NTR) and standard protection (SP), measured at plot scale in Southern Italy during 30 months under natural rainfall. The models were calibrated by adjusting the Curve Numbers (for the SCS-CN model) and the C-factors (MUSLE and USLE-M); the Horton model was not calibrated. The model performance was assessed by qualitative and quantitative procedures. In all practices, the SCS-CN was more accurate for runoff predictions (mean difference of 7% compared with corresponding observations) compared with the Horton (mean difference of 30%). For soil erosion, the MUSLE showed better performance in soils subject to MT or total protection (differences lower than 10%), while the USLE-M was more reliable to simulate soil loss in mulched plots (differences of 8%). A set of Curve Numbers (95 for MT, 70 for SP and 85 for NTR) and C-factors (0.4 for MT, 0.2 for SP and 0.1 for NTR) are proposed for applications in steep slope and clayey soil olive orchards using the SCS-CN and USLE proposed models, respectively. Validation exercises in other environmental experimental conditions would enhance the export these models for runoff and erosion control in agricultural soils treated with mulching.     

High Rate Composting with Innovative Process Control

Despite the variety of composting plants present on the market, the design and operation of composting systems to improve the rate of the process and quality of end products are still an objective that has not been achieved. The composting process evolves through a thermophilic phase followed by a mesophilic one. The metabolism of microorganisms involved in the process is deeply disturbed by environmental changes like temperature, oxygen level and moisture. The aim of this study was to carry out a composting process in two separate reactors: the first, only in the thermophilic phase, with the purpose of pathogen destruction and decomposition by selected thermophilic microorganisms; the second, only in the mesophilic phase, to perform the main microbial transformations with a higher rate of volatile solids destruction. The results confirmed that maintaining constant the main parameters which affect the process, the microbial activity is enhanced. In the two-phase process, the evolution of parameters like moisture, organic carbon, humified organic matter, organic N, C/N and germination index (phytotoxicity) is more rapid relative to the single phase process. Also pathogen destruction is improved with the complete elimination of Faecal coliforms and Enterobacteriaceae and with a stronger reduction of Faecal streptococci.     

Profile of fatty acid lipid fractions of omega-3 fatty acid-enriched table eggs

The omega-3 fatty acid (FA) enrichment of yolk is a key means one of the main objectives to improve the nutraceutical properties of eggs. We evaluated the effect of the dietary inclusion of extruded linseed fed to laying hens on the fatty acid composition of the polar and non-polar lipid classes of the eggs. Two groups of 36 Lohmann White Leghorn layers (65 weeks old) were each fed one of two different diets for a period of 12 weeks. The two diets consisted of a conventional cereal-based diet concentrate (C) and a diet concentrate containing 5% linseed (L). The inclusion of linseed in the diet increased the content of α-linolenic (C18:3n-3), eicosapentaenoic (C20:5n-3) and docosahexaenoic (C22:6n-3) acids in neutral lipids, while a concomitant decrease in arachidonic acid (C20:4n-6) was observed. As regards the polar fraction, the fatty acid composition was slightly affected by the dietary treatments except for C18:0 (+1.14 fold), C18:2n-6 (+1.23 fold), C18:3n-3 (+2.8 fold) and C22:6n-3 (+1.41 fold). Principal component analysis demonstrated that very long-chain FAs were more representative of polar lipids, except for C20:5n-3, while neutral lipids were characterized by dietary n-3 FA (C18:3n-3).     

A statistical approach to evaluate compost genotoxicity

Parallel experiments were carried out to evaluate the induction of genotoxic effects in Vicia faba seedlings treated both with aqueous and organic extracts (dimethyl sulphoxide 5%) of 13 composts, or grown on soil samples to which different doses of the same composts were added. Pearsons linear correlation coefficient and multiple regression were used to study how these two different methodological approaches (treatment with the extracts versus direct contact with the composts in soil) agree. In addition, multiple regression analysis was used to investigate the role of heavy metals and humic substances in genotoxic effects and to construct a model to be used to accurately predict potential genotoxicity of the composts. Experimental data obtained at the highest application rate of compost on soil were found to fit the model and evidenced the role of the humic substances in modulating the potential genotoxicity of the different composts.     

Electrochemical bioassay for the investigation of chlorpyrifos-methyl in vine samples

This paper reports the optimization of an electrochemical bioassay for the determination of chlorpyrifos-methyl and its application to the analysis of grape and vine leaf samples treated with that pesticide. The analytical method was based on electrochemical determination of the extent of the inhibition exerted by the pesticide on acetylcholinesterase using the substrate acetylthiocholine. Two similar calibration plots were obtained, in the range of 1-300 ng/mL, respectively, for chlorpyrifos-methyl in pure standard form and in the commercial preparation Reldan, with comparable coefficients of variation (CV) in the range of 10% < CV < 20%. After an insecticide treatment, samples were analyzed to evaluate its persistence both in grapes and in vine leaves. Samples were evaluated using different extraction procedures: one based on solvent extraction of pesticide residue from grapes and the other based on aqueous extraction from vine leaves using phosphate buffer. The grape solvent extracts were analyzed using both gas chromatography and electrochemical bioassay, whereas the vine leaf buffer extracts were analyzed using the electrochemical bioassay. Quantitative analysis of chlorpyrifos-methyl determined in the two samples, with the electrochemical bioassay, showed a comparable decrease profile over the experimental period.     

Estimating the variance of the sample mean in two-dimensional systematic sampling

This article investigates the problem of estimating the sampling error when the population mean (total) is estimated from a single two-dimensional systematic sample. In particular, two-dimensional extensions of known approximate variance estimators used in linear systematic sampling are introduced. These almost new variance estimators have the advantage of taking into account the spatial ordering of sample units and, consequently, the spatial autocorrelation among them. An investigation of their properties is carried out through a series of simulations and an empirical study.     

Profundal macrobenthos structure as a measure of long-term environmental stress in a polluted lake

Profundal macrobenthic community in a polluted lake (Lake Nemi, Italy) was analyzed in three distinct surveys from 1976 to 1984, in order to define long-term changes in environmental conditions of sediments. Profundal macrobenthos was mainly composed of Oligochaeta. Only a few Chironomidae and a few other taxa were characteristically found in the superior zone. The long-term stress, due to persistent hypolimnetic O₂ deficit, caused progressive simplification of the total community structure. This leads to a high faunal similarity among stations at different depths. The temporal trend of the community structure parameters and the significant role of some taxa (Potamothrix heuscheri, Procladius and Chaoborus flavicans) in the eutrophication process of the lake are also discussed.     

Subtle changes in rhizosphere microbial community structure in response to increased boron and sodium chloride concentrations

Two of the major constraints to grain production in large areas of South-East Australia and cropping soils worldwide are high levels of subsoil boron (B) and excessive salinity (NaCl). Although the effect of these constraints is often studied in plants, the effect on microbially mediated plant-beneficial processes is unclear. To that end, we investigated the impact of B and NaCl on soil microbial community structure (MCS) in the wheat rhizosphere using BIOLOG ecoplates and terminal restriction fragment length polymorphism (T-RFLP). In addition, the effects of B and NaCl on the nitrogen (N) cycle processes of N fixation and ammonia oxidation were assessed by the construction of clone libraries of diazotrophic (nifH) and ammonia oxidising (amoA) rhizobacteria. Analysis of BIOLOG plates using non-metric multidimensional scaling (MDS) revealed addition of both B and NaCl significantly changed MCS, the latter of which was also significant through the analysis of T-RFLP data. Utilisation of several chemical groups of BIOLOG substrates significantly changed in NaCl-amended soil; both B and NaCl affected utilisation of several individual substrates indicative of plant stress including serine and malic acid. A significant decrease in diversity and species richness was observed in high B rhizosphere soil. The community structure of ammonia-oxidising bacteria (AOB), all of which clustered with Nitrosospira-like sequences, did not significantly change in response to addition of B or NaCl, but addition of the latter resulted in a significant increase of diazotroph clones within the α-proteobacteria similar to Azospirillum sp. It appeared that the addition of B and NaCl to soil changed rhizosphere MCS indirectly through increased soil moisture and subtle changes in root exudate patterns, the addition of the latter producing a more distinct change through increased osmotic pressure, leading to a greater increase in rhizodeposition of nutrients, especially carbohydrates. The implications for the current study are that B and NaCl are more likely to affect rhizosphere MCS indirectly through root exudate quantity and/or quality than directly through microbial toxicity, and that plant health is a major determinant in rhizosphere MCS and normal N cycling.