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111.
João Paulo Rodrigues Marques Lilian Amorim Marcel Bellato Spósito Beatriz Appezzato-da-Glória 《European journal of plant pathology / European Foundation for Plant Pathology》2013,135(4):783-790
Postbloom fruit drop (PFD) is caused by both Colletotrichum acutatum and C. gloeosporioides and is a potentially serious disease in citrus that occurs when flowering coincides with rainfall. The fungus incites necrotic lesions in petals and stigmas leading to premature fruit drop and reduced yield. The mechanisms of infection and survival of the causal agents remain to be fully elucidated. In the present study, we investigated the histopathology of PFD caused by C. acutatum in the petals and stigmas of sweet oranges using electron and light microscopy. In the petals, pathogen penetration occurred intra and intercellularly and also through the stomata, with intercellular penetration occurring most frequently. The distinct tissues of the petals were colonised, including the vascular system, particularly the xylem. Acervuli were observed on both sides of the petals. Although the fungus did not penetrate through the epidermal cells of the stigma, C. acutatum caused necrosis and an increase of phenolics in this tissue. A protective layer rich in lipophilic and phenolic compounds was formed under the necrotic area and crystals of oxalate were associated with the sites where the pathogen was present. 相似文献
112.
João Paulo Rodrigues Marques Lilian Amorim Marcel Bellato Spósito Denis Marin Beatriz Appezzato-da-Glória 《European journal of plant pathology / European Foundation for Plant Pathology》2013,136(1):35-40
Postbloom fruit drop (PFD), an important disease caused by Colletotrichum spp., affects citrus yields in Brazil. PFD is characterised by the presence of necrotic lesions on the petals and stigmas of citrus flowers and by the subsequent abscission of young fruit. PFD epidemics have high disease progress rates, which is unusual for a pathogen that produces acervuli and is dispersed by rain. It is possible that other dispersal agents, such as insects and pollen, are involved in the spread of this disease. The objective of this work was to test whether citrus pollen grains can be colonised by Colletotrichum acutatum. Studies using light and electron microscopy showed that the pollen of Citrus sinensis can be infected by C. acutatum. This pathogen can penetrate and colonise citrus pollen grains 24 h after inoculation with the pathogen. The germ tube of conidia either penetrates the pollen sporodermis directly or passes through pollen germ pores. A single hypha can colonise more than one pollen grain. On the surface of the stigma, conidium formation can be observed. This study shows that Citrus sinensis pollen may, in fact, play a role in the spread of C. acutatum in citrus orchards. 相似文献
113.
Red lead (Pb3O4)‐ and ZnO‐containing anticorrosion paints in the past have been extensively applied to high‐voltage steel pylons which has led to heavy metal (HM) soil contaminations in their vicinity. Since pylons are commonly found on agricultural land, there is a potential risk of HM plant uptake. This is promoted by the fact that in contrast to the moderate total Pb contents (several 100 mg kg–1) in three nutrient‐poor and acidic pylon soils the Pb amounts extractable with NH4NO3 were extremely high, reaching almost 20% of total Pb. A 18‐week field pot trial (three harvests in a six‐week interval) using Lolium multiflorum was conducted to study the HM plant uptake and the efficiency of the four soil additives, lime (LI), Novaphos (NP), water‐treatment sludge (WS), and ilmenite residue (IR) in reducing the plant uptake and NH4NO3‐extractability of Pb and Zn in the soils. Lead concentrations in L. multiflorum shoots grown in the untreated soils reached maximum values of 128 mg (kg dry weight)–1. Novaphos was most efficient in decreasing shoot Pb (–90%) followed by LI (–78%) and WS (–73%). For Zn, too, LI (–82%), NP, and WS (both –66%) substantially reduced plant uptake. Ilmenite residue was generally only poorly efficient. The dry‐matter yield in the NP, LI, and WS treatments was significantly increased. While the relationship between Pb‐NH4NO3 and Pb‐plant was high when considering the three harvests separately (R > 0.93) a poor relationship (R = 0.63) exists over all harvests together. This was attributed to different transpiration rates affecting the HM flux into the plants, since the temperature regime changed greatly during the cultivation period. For Zn, no such close relationship between the NH4NO3‐extractable soil fraction and shoot Zn was found, most likely due to antagonistic effects from Mg which greatly varied in the three soils. 相似文献
114.
Bertalan Juhasz Dawrin Pech-Puch Jioji N. Tabudravu Bastien Cautain Fernando Reyes Carlos Jimnez Kwaku Kyeremeh Marcel Jaspars 《Marine drugs》2021,19(6)
Three dermacozines, dermacozines N–P (1–3), were isolated from the piezotolerant Actinomycete strain Dermacoccus abyssi MT 1.1T, which was isolated from a Mariana Trench sediment in 2006. Herein, we report the elucidation of their structures using a combination of 1D/2D NMR, LC-HRESI-MSn, UV–Visible, and IR spectroscopy. Further confirmation of the structures was achieved through the analysis of data from density functional theory (DFT)–UV–Visible spectral calculations and statistical analysis such as two tailed t-test, linear regression-, and multiple linear regression analysis applied to either solely experimental or to experimental and calculated 13C-NMR chemical shift data. Dermacozine N (1) bears a novel linear pentacyclic phenoxazine framework that has never been reported as a natural product. Dermacozine O (2) is a constitutional isomer of the known dermacozine F while dermacozine P (3) is 8-benzoyl-6-carbamoylphenazine-1-carboxylic acid. Dermacozine N (1) is unique among phenoxazines due to its near infrared (NIR) absorption maxima, which would make this compound an excellent candidate for research in biosensing chemistry, photodynamic therapy (PDT), opto-electronic applications, and metabolic mapping at the cellular level. Furthermore, dermacozine N (1) possesses weak cytotoxic activity against melanoma (A2058) and hepatocellular carcinoma cells (HepG2) with IC50 values of 51 and 38 μM, respectively. 相似文献
115.
Crispness of bread is rapidly lost because of water migration inside the crumb towards the crust. How crumb properties determine this process independent of crust properties has not been examined before. Therefore, the aim of this study was to analyze and explain the influence of crumb morphology on the overall crispness retention. Crispness retention was determined by analyzing the acoustic emission of breads differing in either crust or crumb morphology. When crumb morphology is coarse with a lower number of large connections between the air cells, the effective diffusion coefficient is reduced. This effective diffusion coefficient of crumb, which equals approximately half the value of air, was estimated using X-ray micro-computer tomography images of crumb pieces. If the crumb has a lower effective diffusion coefficient, bread with similar crust properties has significantly longer crispness retention. Despite this, our data show that variations in properties of crust, which has 30 times higher permeability than crumb, have a larger impact on crispness retention than variations in properties of crumb. 相似文献
116.
Yellow passion fruit (Passiflora edulis f. flavicarpa Degener) rind pectic substances were fractionated with water, ammonium oxalate, and dilute acid solutions. The extracted pectins were rich in anhydrogalacturonic acid and had a low degree of methyl esterification. Moreover, their acetyl groups and neutral sugar contents were relatively low. Furthermore, a low amount of proteinaceous material was also found within them. Their gelling ability and viscoelastic properties as evaluated by the SAG and small amplitude oscillatory shear tests, respectively, were comparable to those of a commercial citrus low-methoxyl pectin. Hence, yellow passion fruit rind occurs as a potentially good source of naturally low-methoxyl pectin. 相似文献
117.
Eveline C. van der Deijl Marcel van der Perk Hans Middelkoop 《Journal of Soils and Sediments》2017,17(11):2620-2636
Purpose
A thorough understanding of mechanisms controlling sedimentation and erosion is vital for a proper assessment of the effectiveness of delta restoration. Only few field-based studies have been undertaken in freshwater tidal wetlands. Furthermore, studies that measured sediment deposition in newly created wetlands are also sparse. This paper aims to identify the factors controlling the sediment trapping of two newly created freshwater tidal wetlands.Materials and methods
Two recently re-opened polder areas in the Biesbosch, The Netherlands are used as study area. Field measurements of water levels, flow velocities, and turbidity at both the in- and outlet of the areas were carried out to determine the sediment budgets and trapping efficiencies under varying conditions of river discharge, tide, and wind in the period 2014–2016.Results and discussion
Short-term sediment fluxes of the two study areas varied due to river discharge, tide, and wind. A positive sediment budget and trapping efficiency was found for the first study area, which has a continuing supply of river water and sediment. Sediment was lost from the second study area which lies further from the river and had a lower sediment supply. The daily sediment budget is positively related to upstream river discharge, and in general, export takes place during ebb and import during flood. However, strong wind events overrule this pattern, and trapping efficiencies decrease for increasing wind strengths at mid-range river discharges and for the highest river discharges due to increased shear stress.Conclusions
Delta restoration, based on sedimentation to compensate for sea-level rise and soil subsidence, could only be effective when there is a sufficient supply of water and sediment. Management to enhance the trapping efficiency of the incoming sediment should focus on directing sufficient river flow into the wetland, ensuring the supply of water and sediment within the system during a tidal cycle, creating sufficiently large residence time of water within the polder areas for sediment settling, and decreasing wave shear stress by the establishment of vegetation or topographic irregularities.118.
Fernando Scala Ernesto Fattorusso Marialuisa Menna Orazio Taglialatela-Scafati Michelle Tierney Marcel Kaiser Deniz Tasdemir 《Marine drugs》2010,8(7):2162-2174
In the present study, 13 bromopyrrole alkaloids, including the oroidin analogs hymenidin (2), dispacamide B (3) and dispacamide D (4), stevensine (5) and spongiacidin B (6), their derivatives lacking the imidazole ring bromoaldisin (7), longamide B (8) and longamide A (9), the dimeric oroidin derivatives sceptrin (10) and dibromopalau’amine (11), and the non-oroidin bromopyrrolohomoarginin (12), manzacidin A (13), and agelongine (14), obtained from marine sponges belonging to Axinella and Agelas genera have been screened in vitro against four parasitic protozoa, i.e., two Trypanosoma species (T. brucei rhodesiense and T. cruzi), Leishmania donovani and Plasmodium falciparum (K1 strain, a chloroquine resistant strain), responsible of human diseases with high morbidity and, in the case of malaria, high mortality. Our results indicate longamide B (8) and dibromopalau’amine (11) to be promising trypanocidal and antileishmanial agents, while dispacamide B (3) and spongiacidin B (6) emerge as antimalarial lead compounds. In addition, evaluation of the activity of the test alkaloids (2–14) against three different enzymes (PfFabI, PfFabG, PfFabZ) involved in the de novo fatty acid biosynthesis pathway of P. falciparum (PfFAS-II) identified bromopyrrolohomoarginin (12) as a potent inhibitor of PfFabZ. The structural similarity within the series of tested molecules allowed us to draw some preliminary structure-activity relationships. Tests against the mammalian L6 cells revealed important clues on therapeutic index of the metabolites. This is the first detailed study on the antiprotozoal potential of marine bromopyrrole alkaloids. 相似文献
119.
120.
Yangyang JIA Marcel G. A. VAN DER HEIJDEN Alain Y. VALZANO-HELD Markus JOCHER Florian WALDER 《土壤圈》2024,34(2):399-410
Nitrogen (N) is one of the most limited nutrients of terrestrial ecosystems, whose losses are prevented in tightly coupled cycles in finely tuned systems. Global change-induced N enrichment through atmospheric deposition and application of vast amounts of fertilizer are now challenging the terrestrial N cycle. Arbuscular mycorrhizal fungi (AMF) are known drivers of plant-soil nutrient fluxes, but a comprehensive assessment of AMF involvement in N cycling under global change is still lacking. Here, we simulated N enrichment by fertilization (low/high) in experimental grassland microcosms under greenhouse conditions in the presence or absence of AMF and continuously monitored different N pathways over nine months. We found that high N enrichment by fertilization decreased the relative abundance of legumes and the plant species dominating the plant community changed from grasses to forbs in the presence of AMF, based on aboveground biomass. The presence of AMF always maintained plant N:phosphorus (P) ratios between 14 and 16, no matter how the soil N availability changed. Shifts in plant N:P ratios due to the increased plant N and P uptake might thus be a primary pathway of AMF altering plant community composition. Furthermore, we constructed a comprehensive picture of AMF’s role in N cycling, highlighting that AMF reduced N losses primarily by mitigating N leaching, while N2O emissions played a marginal role. Arbuscular mycorrhizal fungi reduced N2O emissions directly through the promotion of N2O-consuming denitrifiers. The underlying mechanism for reducing N leaching is mainly the AMF-mediated improved nutrient uptake and AMF-associated microbial immobilization. Our results indicate that synergies between AMF and other soil microorganisms cannot be ignored in N cycling and that the integral role of AMF in N cycling terrestrial ecosystems can buffer the upcoming global changes. 相似文献