Effects of neem (Azadirachta indica) seed kernel extracts from different solvents on the carmine spider mite,Tetranychus cinnabarinus

Extracts of neem (Azadirachta indica A. Juss) seed kernels prepared from various solvents affected the behavior and fecundity of the carmine spider mite,Tetranychus cinnabarinus (Boisd.). Bean leaf discs freshly sprayed with different concentrations of the extracts strongly repelled the females from the treated leaves and egg-laying was reduced. On the basis of EC 50s for both parameters, the order of effectiveness of the extracts was pentane > chloroform >n-butanol > acetone > methanol > H₂ 0 (inactive). The activity decreased with increasing dielectric constant of the extracting solvent. The chloroform and butanol extracts in the original solvents were phytotoxic. Bean leaf discs 7 days after spraying with the pentane and acetone extracts, still caused mortality and reduction in fecundity of adult females, but no repellency. The acetone and the pentane extracts sprayed directly on adult female mites on bean leaf discs, caused repellency and reduction of fecundity and also mortality of adults.     

Effect of commonly used pesticides onChiracanthium mildei and other spiders occurring on apple

Phytoparasitica - Laboratory experiments employing two test procedures were carried out to evaluate the effect of three commercial pesticides commonly used against apple pests in Israel, on the...     

Acute and Chronic Effects of a Contraceptive Compound RTI‐4587‐073(l) on Testicular Histology and Endocrine Function in Miniature Horse Stallions

The objective of this study was to evaluate acute endocrine effects as well as histological changes in testicular parenchyma induced by the contraceptive compound RTI‐4587‐073(l). Six miniature stallions were used in this experiment. The treatment group (n = 3) received one oral dose of 12.5 mg/kg of RTI‐4587‐073(l), and the control group (n = 3) received placebo only. The stallions' baseline parameters (semen, testicular dimensions, endocrine values) were collected and recorded for 5 weeks before treatment and for 6 weeks after treatment. Multiple blood samples were collected for endocrine analysis. Testicular biopsies were obtained before treatment, 1 day after treatment and every other week after treatment. Ultrasound exams were performed to monitor the dimensions of the stallions' testes. All stallions were castrated 6 weeks after treatment. Sperm numbers, motility and percentage of morphologically normal sperm decreased (p < 0.05), while the number of immature germ cells increased in ejaculates from treated animals (p < 0.05). Serum concentrations of inhibin and follicle‐stimulating hormone did not change. Testosterone concentrations initially transiently decreased (p < 0.05) after administration of RTI‐4587‐073(l), and increased several days later (p < 0.05). Testicular content of testosterone and estradiol 17‐β was lower in treated stallions than in control stallions on Day 1 after treatment (p < 0.05). Severe disorganization of the seminiferous tubules, significant loss of immature germ cells and complete depletion of elongated spermatids were observed in testicular biopsies obtained from treated stallions 1 day, 2 and 4 weeks after treatment. These changes were still present in the testicular samples taken from treated stallions after castration. The results of this study confirmed that RTI‐4587‐073(l) has antispermatogenic effects in stallions. Furthermore, we concluded that this compound causes acute sloughing of immature germ cells from the seminiferous tubules. RTI‐4587‐073(l) has significant but transient effects on Leydig cell function in stallions.     

Association of a novel SNP in exon 10 of the IGF2 gene with growth traits in Egyptian water buffalo (Bubalus bubalis)

Insulin-like growth factor 2 (IGF2) plays an important role in muscle growth and it might be used as a marker for the growth traits selection strategies in farm animals. The objectives of this study were to detect polymorphisms in exon 10 of IGF2 and to determine associations between these polymorphisms and growth traits in Egyptian water buffalo. PCR-single-strand conformation polymorphism (SSCP) and DNA sequencing methods were used to detect any prospective polymorphism. A novel single nucleotide polymorphism (SNP), C287A, was detected. It was a non-synonymous mutation and led to replacement of glutamine (Q) amino acid (aa) by histidine (H) aa. Three different SSCP patterns were observed: AA, AC, and CC, with frequencies of 0.540, 0.325, and 0.135, respectively. Association analyses revealed that the AA individuals had a higher average daily gain (ADG) than other individuals (CC and AC) from birth to 9 months of age. We conclude that the AA genotype in C287A SNP in the exon 10 of the IGF2 gene is associated with the ADG during the age from birth to 9 months and could be used as a potential genetic marker for selection of growth traits in Egyptian buffalo.     

The 18O/16O and 17O/16O ratios in atmospheric nitrous oxide: A mass-independent anomaly

Measurements of the oxygen isotope ratios (18O/16O and 17O/16O) in atmospheric nitrous oxide (N2O) from La Jolla, Pasadena, and the White Mountain Research Station (elevation, 3801 meters) in California and the White Sands Missile Range in New Mexico show that N2O has a mass-independent composition. These data suggest the presence of a previously undefined atmospheric process. The La Jolla samples can be explained by a mixing between an atmospherically derived source of mass-independent N2O and biologically derived mass-dependent N2O. Possible origins of the mass-independent anomaly in N2O are discussed.     

Production of low-fat cookies and their nutritional and metabolic effects in rats

Two weaning formulations were designed using computer software and then processed on a pilot plant scale by three different schemes involving extrusion cooking and traditional food processes such as soaking, decorticating and toasting to yield a total of six precooked experimental mixtures. The ingredients incorporated into these formulations were maize, cowpeas, peanuts, soybeans and soybean oil. Proximate composition indicated protein and lipid contents of 17.5-20.0 g/100 g and 7.8-9.1 g/100 g, respectively. Mineral analyses performed on samples indicated that the concentrations of copper, magnesium and zinc were comparable to those predicted whereas the concentrations of calcium and iron were lower than had been expected. Thiamin, riboflavin and niacin content ranged between 50-80% of the values predicted by the software. The differences could be due to varietal differences inherent in the nutrient profile of the raw materials or a result of processing these mixtures. However, a 100 g portion of all the supplements met at least one-third of the daily nutrient requirement of the 0.5-0.9 year old infant for selected micronutrients.     

Enriched Artemia with L-lysine and DL-methionine on growth performance,stress resistance,and fatty acid profile of Litopenaeus vannamei postlarvae

A 15-day study was conducted to evaluate the effect of enrichment of Artemia metanauplii with four different emulsions, including: (A) Easy-DHA Selco; (B) Easy-DHA Selco + L-lysine (4 g/L); (C) Easy-DHA Selco + DL-methionine (4 g/L); and (D) Easy-DHA Selco + L-lysine (2 g/L) + DL-methionine (2 g/L) on growth and stress resistance of white leg shrimp Litopenaeus vannamei larvae (from Mysis I to postlarvae12). It is noticed that shrimp PL in group B had the highest wet (36.1 ± 3.1 mg) and dry (1116.7 ± 60.0 µg) weights as well as stress resistance to fresh water (64.0 ± 5.5%).     

Identification of lactoferrin and glutamate receptor‐interacting protein 1 in bovine cervical mucus: A putative marker for oestrous detection

Accurate detection of oestrus is important for artificial insemination. The aim of this study was to identify oestrous‐specific bovine cervical mucus proteins that could be used to determine the optimal time for artificial insemination. Non‐oestrous and controlled internal drug release (CIDR)‐induced oestrous‐stage mucus proteins were purified and subjected to surface‐enhanced laser desorption/ionization time‐of‐flight mass spectrometry, sodium dodecyl sulphate polyacrylamide gel electrophoresis and MALDI‐TOF/TOF. Among differentially expressed proteins, lactoferrin (LF) and glutamate receptor‐interacting protein 1 (GRIP1) showed a twofold increase during the CIDR‐induced oestrous stage compared to the levels in non‐oestrous stage in bovine cervical mucus. The RT‐PCR, Western blotting and immunohistochemistry results showed that LF and GRIP1 expression was significantly increased during the oestrous stage in the uterus. This study demonstrated that bovine LF and GRIP1 exist during the oestrous stage, but not during the non‐oestrous stage, suggesting that cervical mucus LF and GRIP1 are useful oestrous detection markers in cattle.     

Distribution changes of woody plants in Western Iran as monitored by remote sensing and geographical information system: a case study of Zagros forest

The status of woody plants in dry-land systems is a fundamental determinant of key ecosystem processes. Monitoring of this status plays an important role in understanding the dynamics of woody plants in arid and semi-arid ecosystems. The present study determined the dynamism of the Zagros forests in Iran using Remote Sensing and Geographic Information System techniques and statistical science. The results show that the density of trees varied from 10 to 53 % according to the physiographic and climatic conditions of semi-arid regions. The best and lowest correlation between vegetation indices and forest density were obtained for the global environmental monitoring index (GEMI; R ² = 0.94) and soil adjust vegetation index (R ² = 0.81), respectively. GEMI is used to monitor land use changes over a 10-year period. Results show that 2720 ha² of forest have been destroyed by human interference and tillage on steep slopes during this period which also resulted in the loss of the fertile soil layer. GEMI determined the areas with a biomass of trees and could normally separate border regions with low biomass density of trees from regions without canopy cover. The results revealed that assessment of forest and vegetation cover in arid and semi-arid arduous forest regions using satellite digital numbers and ordinary sampling is subject to uncertainty. A stratified grouping procedure should be established to increase the accuracy of assessment.