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61.
Nakata M Sakai M Sakai T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1223-1224
A crossbred Maltese dog, 6-year-old, male, was presented to us for examination due to coagulopathy. On examination of blood coagulation screening tests, activated partial thromboplastin time (APTT) was markedly prolonged (63.6 sec). Therefore, a defect in the intrinsic pathway of coagulation was suspected. An additional serum test was also examined and APTT was returned to within the normal range. Furthermore, factor IX coagulation activity was markedly low (2.3%). On the basis of these results, the dog was diagnosed with hemophilia B. The dog has since been presented to us because of hemorrhage problems again after 5, 10, and 16 months, but blood transfusions have maintained good control of its coagulopathy for more than two years. 相似文献
62.
63.
Sakai M Otani I Ishigaki K Edamura K Takahashi T Koie H Yamaya Y Watari T Shibuya H Sato T Kanayama K Tokuriki M Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1219-1221
We evaluated hepatic T lymphocyte phenotypes in a dog with chronic hepatitis. Before treatment, numerous CD3+ lymphocytes were demonstrated in the liver, and the ratio of CD4+/CD8+ was remarkably high (2.96; reference range, 0.33+/-0.12). After treatment, CD3+ lymphocyte infiltration in the liver was reduced, and the ratio of CD4+/CD8+ decreased to 0.31. Therefore, hepatic T lymphocytes, especially CD4+ lymphocytes, might play a central role in the pathogenesis of this dog with chronic hepatitis. 相似文献
64.
C Pöhlker KT Wiedemann B Sinha M Shiraiwa SS Gunthe M Smith H Su P Artaxo Q Chen Y Cheng W Elbert MK Gilles AL Kilcoyne RC Moffet M Weigand ST Martin U Pöschl MO Andreae 《Science (New York, N.Y.)》2012,337(6098):1075-1078
The fine particles serving as cloud condensation nuclei in pristine Amazonian rainforest air consist mostly of secondary organic aerosol. Their origin is enigmatic, however, because new particle formation in the atmosphere is not observed. Here, we show that the growth of organic aerosol particles can be initiated by potassium-salt-rich particles emitted by biota in the rainforest. These particles act as seeds for the condensation of low- or semi-volatile organic compounds from the atmospheric gas phase or multiphase oxidation of isoprene and terpenes. Our findings suggest that the primary emission of biogenic salt particles directly influences the number concentration of cloud condensation nuclei and affects the microphysics of cloud formation and precipitation over the rainforest. 相似文献
65.
Manabu Nemoto Hiroshi Tsunemitsu Harutaka Murase Yasuo Nambo Shinsuke Sato Yasuhiro Orita Hiroshi Imagawa Hiroshi Bannai Koji Tsujimura Takashi Yamanaka Tomio Matsumura Takashi Kondo 《Acta veterinaria Scandinavica》2012,54(1):63
Background
Both the G3P[12] and the G14P[12] type of equine group A rotavirus (RVA) have recently become predominant in many countries, including Japan. G3 types are classified further into G3A and G3B. The G3A viruses have been circulating in Europe, Australia, and Argentina, and the G3B viruses have been circulating in Japan. However, only an inactivated vaccine containing a single G3BP[12] strain is commercially available in Japan. To assess the efficacy of the current vaccine against recently circulating equine RVA strains, we examined antibody responses in pregnant mares to recent G3BP[12] and G14P[12] strains by virus neutralization test.Findings
After vaccination in five pregnant mares, the geometric mean serum titers of virus-neutralizing antibody to recent G3BP[12] strains increased 5.3- to 7.0-fold and were similar to that against homologous vaccine strain. Moreover, antibody titers to recent G14P[12] strains were also increased 3.0- to 3.5-fold.Conclusions
These results suggest that inoculation of mares with the current vaccine should provide foals with virus-neutralizing antibodies against not only the G3BP[12] but also the G14P[12] RVA strain via the colostrum. 相似文献66.
We studied the longevity of mice produced without sperm using the genomes of oocytes that are already committed to a germline cell lineage. The first sperm-free mouse "KAGUYA", which we term 'bi-maternal mouse', was born on 3 February, 2003. Bi-maternal embryos were generated using 2 sets of female genomes--one derived from fully grown oocytes from normal adults and the other from non-growing oocytes from newborn pups. These genomes were combined by nuclear transfer. We refined the technique for generating bi-maternal mice and found that genetic manipulations in only 2 regions--the imprinting centres of Igf2-H19 and Dlk1-Gtl2--on chromosomes 7 and 12 of the newborn pups allowed us to generate bi-maternal mice at a high rate. Studying bi-maternal conceptuses and mice provides further insight into the mechanisms by which paternally methylated imprinted genes regulate mammalian ontogenesis. 相似文献
67.
Bannai H Tsujimura K Kondo T Nemoto M Yamanaka T Sugiura T Kato T Maeda K Matsumura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(6):791-795
The immunoglobulin G (IgG) subclass response was investigated in horses with or without pyrexia after natural infection with equine herpesvirus type 1 (EHV-1) in the field. All horses were kept at the training centers of the Japan Racing Association and were immunized with an inactivated EHV-1 vaccine before EHV-1 infection. An IgG subclass response dominated by IgGa and IgGb was induced in horses without pyrexia after EHV-1 infection. In contrast, horses that developed pyrexia showed increased IgGc and IgG (T) subclass production in addition to IgGa and IgGb. Although inactivated EHV-1 vaccines are considered to induce a mainly Th-2-biased response, these results indicated that the responses in horses inoculated with inactivated EHV-1 vaccine were not uniform, and that horses with a Th-1-biased response were likely to be protected from pyrexia. 相似文献
68.
Yamanaka T Bannai H Nemoto M Tsujimura K Kondo T Muranaka M Hobo S Minamijima YH Yamada M Matsumura T 《Veterinary journal (London, England : 1997)》2012,193(2):358-362
Equine influenza A virus (EIV) of the H3N8 subtype is an important pathogen causing acute respiratory disease in horses. Peramivir is a selective inhibitor of the influenza virus neuraminidase (NA). The characteristics of peramivir are not only its capacity for parenteral administration, but also its strong affinity for NA and slow off-rate from the NA-peramivir complex, suggesting that it could lead to a prolonged inhibitory effect and thus allow a lower dosing frequency. The aims of this study were to evaluate the inhibitory efficacy of peramivir against the NA activities of EIV in vitro and the treatment efficacy of a single intravenous dose of peramivir in horses experimentally infected with EIV. Peramivir inhibited the activities of NA from the seven contemporary EIV strains in vitro, with 50% inhibitory concentrations ranging from 0.10 to 0.20nmol/L. Horses treated with a single IV dose of peramivir (3000mg/600mL/animal, 7.8-9.3mg/kg of bodyweight) showed significantly milder clinical signs (pyrexia, nasal discharge and cough) with a shorter duration than control horses injected with normal saline. Moreover, the mean duration of virus shedding for the horses treated with peramivir was significantly shorter than for the control horses. These findings suggested that a single IV administration of peramivir had good potential for the treatment of equine influenza, and may help to limit the spread of the disease in the horse population. 相似文献
69.
Feline gastrointestinal eosinophilic sclerosing fibroplasia was diagnosed in an 8-month-old Scottish fold that had a primary gastrointestinal mass involving the stomach, duodenum and mesenteric lymph nodes. Histopathologically, the most characteristic feature of this mass was granulation tissue with eosinophil infiltration and hyperplasia of sclerosing collagen fiber. Immunohistochemically, large spindle-shaped cells were positive for smooth muscle actin and vimentin. This case emphasizes the importance of feline gastrointestinal eosinophilic sclerosing fibroplasia as a differential diagnosis of gastrointestinal neoplastic lesions such as osteosarcoma and mast cell tumor in cats. 相似文献
70.
Environmental controls on photosynthetic production and ecosystem respiration in semi-arid grasslands of Mongolia 总被引:3,自引:0,他引:3
Tomoko Nakano Manabu Nemoto Masato Shinoda 《Agricultural and Forest Meteorology》2008,148(10):1456-1466
The Mongolian steppe zone comprises a major part of East Asian grasslands. The objective of this study was to separately evaluate the quantitative dependencies of gross primary production (GPP) and ecosystem respiration (Reco) on the environmental variables of temperature, moisture, radiation, and plant biomass in a semi-arid grassland ecosystem. We determined GPP and Reco using transparent and opaque closed chambers in a grassland dominated by Poaceae species in central Mongolia during five periods: July 2004, May 2005, July 2005, September 2005, and June 2006. Values of GPP were linearly related to live aboveground biomass (AGB) enclosed by the chamber. The amount of GPP per unit ground area differed among the study periods, whereas GPP normalized by the amount of AGB did not differ significantly among the periods, suggesting that plant production per unit green biomass did not depend on the phenological stage. GPP/AGB fit well a rectangular hyperbolic light–response curve for all the study periods. When the air and soil were dry, considerable reduction in GPP was observed. The GPP/AGB ratio was also expressed as individual functions of air temperature, vapor pressure deficit, and volumetric soil water content. Reco was exponentially related to the soil temperature and the relationship was modified by soil moisture. The amount of Reco and its temperature sensitivity (Q10) declined with decreasing soil moisture. Sharp increases of Reco after rainfall events were observed. The values of Reco, even including the rain-induced pulses, were expressed well as a bivariate function of soil temperature and soil moisture near the soil surface. 相似文献