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31.
Zhao Z Shin HS Satsu H Totsuka M Shimizu M 《Journal of agricultural and food chemistry》2008,56(10):3863-3868
Although chlorogenic acid (CHA) easily reaches a millimolar level in the gastrointestinal tract because of its high concentration in coffee and fruits, its effects on intestinal epithelial cells have been little reported. We investigated in this study the down-regulative effects of 5-caffeoylquinic acid (CQA), the predominant isomer of CHA, on the H(2)O(2-) or TNF-alpha-induced secretion of interleukin (IL)-8, a central pro-inflammatory chemokine involved in the pathogenesis of inflammatory bowel diseases, in human intestinal epithelial Caco-2 cells. After the cells had been pre- and simultaneously treated with CQA, the oversecretion of IL-8 and overexpression of its mRNA induced by H(2)O(2) were significantly suppressed in a dose-dependent manner in the range of 0.25-2.00 mmol/L. We further found that a metabolite of CQA, caffeic acid (CA), but not quinic acid, significantly inhibited the H(2)O(2)-induced IL-8 secretion and its mRNA expression in the same dose-dependent manner. Both CQA and CA suppressed the TNF-alpha-induced IL-8 secretion as well. Caffeic acid at 2.00 mmol/l was able to absolutely block the H(2)O(2)- or TNF-alpha-induced oversecretion of IL-8 in Caco-2 cells. However, CQA and CA did not suppress the TNF-alpha-induced increase in the IL-8 mRNA expression, indicating that the suppressive mechanisms are different between TNF-alpha-induced and H(2)O(2)-induced IL-8 production models. These results suggest that the habit of drinking coffee and/or eating fruits with a high CHA content may be beneficial to humans in preventing the genesis of inflammatory bowel diseases. 相似文献
32.
Mamoru?SatoEmail author Wei?Wei Kenji?Watanabe 《Journal of General Plant Pathology》2003,69(6):391-396
A mulberry epiphytic Enterobacter cloacae MUL1 harbors plasmid pMUL1 encoding five drug-resistance genes. This plasmid was examined upon its conjugal transfer into epiphytic Erwinia herbicola on the phylloplane of mulberry and 12 species of weeds. The plasmid was transferred into Er. herbicola at a frequency of 10–5–10–3/recipient in mulberry and Lolium multiflorum LAM. 1–8 days after wound inoculation with 106–108/ml suspensions. In Chenopodium album L. and C. album L. var. centrorubrum, however, it was transferred only after wound inoculation with a 108/ml suspension, but not with 107/ml or 106/ml suspensions, owing to the weak epiphytic fitness of Ent. cloacae on these weeds. Transconjugants were also obtained for seven other species of weeds in the case of inoculation with a 108/ml suspension. In contrast, when bacterial suspensions were sprayed on mulberry leaves with or without fresh wounds, transconjugants were obtained only in wounded leaves, which were considered suitable for bacterial conjugation. These findings suggest that epiphytic bacteria, including Ent. cloacae and Er. herbicola, may be carriers of drug-resistance genes distributed among plant pathogenic bacteria in nature. 相似文献
33.
Noriko SHIOMI-SUGAYA Kouji KOMATSU Jingwen WANG Mamoru YAMASHITA Fumitaka KIKKAWA Akira IWASE 《The Journal of reproduction and development》2015,61(3):161-168
Ovaries contain follicles at various stages of development, including primordial, primary, secondary, antral and Graafian follicles. Although the growth of these follicles is controlled to maintain regular ovulation, the mechanism through which this occurs remains unclear. In our study, we found that the growth rate of cultured secondary follicles separated from mice ovaries differed between follicles. After 4 days of culture, the size of some secondary follicles was markedly increased, while that of others had either slightly increased, remained unchanged or shrunk. We compared the expression levels of growth factors between these secondary follicles and found that the growth rate of cultured secondary follicles correlated with the expression level of insulin-like growth factor 1 (Igf1) mRNA. Igf1 mRNA expression level in secondary follicles containing theca cells was higher than that in secondary follicles without theca cells, and the
granulosa cell proliferation around follicles containing theca cells was increased. Furthermore, an IGF1 inhibitor also inhibited the granulosa cell proliferation, and administration of IGF1 to secondary follicles without growth promoted granulosa cell proliferation. These results indicated that the theca cells of secondary follicles induced the expression of IGF1 and promoted the follicle growth. 相似文献
34.
Hiroshi OKAMOTO Mamoru SATO Yoshio MIYATA Masami YOSHIKAWA Makoto ISAKA 《Journal of General Plant Pathology》2000,66(1):86-94
Bacterial strains isolated from the rhizosphere of angelica trees were evaluated for their antagonistic activity against Phytophthora cactorum, a causal agent of Phytophthora root rot. Of these, three bacterial strains, designated as T-1-8, T-1-14 and T-1-23, strongly inhibited mycelial growth of
P. cactorum ARE-862 in a dual-culture plate assay. Biocontrol activity of these strains was then examined by dipping root of young seedlings
of angelica trees into a bacterial suspension. The incidence of Phytophthora root rot was markedly suppressed for at least 79 days in pot tests when treated seedlings were planted in naturally infested
soil. The suppression was maintained through June of the next year. In addition, these strains significantly reduced the development
of Phytophthora root rot up to 47 days in naturally infested field and up to 63 days (the last day of testing) in an artificially (moderately)
infested field. Based on their main bacteriological properties, strain T-1-14 was identified as Enterobacter cloacae and T-1-8 and T-1-23 were identified as Serratia ficaria.
Received 5 July 1999/ Accepted in revised form 25 October 1999 相似文献
35.
Several epiphytic strains of Enterobacter cloacae isolated from mulberry leaves were resistant to antibiotics such as streptomycin, kanamycin, ampicillin, tetracycline and
chloramphenicol, and harbored a 100-kb plasmid designated pMUL1. Plasmid profile analysis of spontaneous mutants derived from
Ent. cloacae MUL1 and MUL1 (RSF1010) suggested that pMUL1 confers resistance to multiple antibiotics. Southern blot analysis using probes
of five antibiotic-resistance genes against EcoRI-digested DNA from pMUL1
and defective pMUL1s (mutants) revealed that all these genes were located within a 24-kb region of pMUL1 and that some genes
were assigned to the defective plasmids. A similar antibiotic-resistance plasmid was detected in several orchid-pathogenic
strains of Ent. cloacae, but not in the type-culture strain (JCM 1232) or strains of Ent. cloacae of insect-origin. Strains MUL1 and MUL1 (RSF1010) were then mated with epiphytic Erwinia spp. and Pseudomonas spp. on filters, respectively. Several recipient strains of epiphytic Er. herbicola simultaneously acquired plasmid pMUL1 and the phenotype of multiple-antibiotic resistance. Thus, pMUL1 was verified to be
conjugative and to encode genes for multiple-antibiotic resistance, including genes homologous to the strA-strB of the nonconjugative IncQ plasmid RSF1010. These findings suggest that epiphytic Ent. cloacae may play a role in the dissemination of antibiotic-resistance genes among epiphytic bacteria and plant pathogenic bacteria.
Received 10 January 2002/ Accepted in revised form 29 March 2002 相似文献
36.
Ageyama N Hanazono Y Shibata H Ono F Nagashima T Ueda Y Yoshikawa Y Hasegawa M Ozawa K Terao K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(5):507-510
Genes and proteins of human origin are often administered to monkeys for research purposes, however, it can be difficult to obtain sufficient levels of the products in vivo due to immunological clearance. In this study, we showed that human erythropoietin (hEPO) induces generation of anti-hEPO antibody in cynomolgus macaques (n=2), although 92% of amino acid residues are common between the human and macaque EPO. The administered hEPO was thus eliminated from the animals. On the other hand, when an immunosuppressant, cyclosporin A (CyA), was administered (6 mg/kg) intramuscularly every other day in combination with hEPO (n=2), no anti-hEPO antibody was generated and high serum levels of hEPO were obtained during administration of hEPO, resulting in an increase in serum hemoglobin levels. No adverse effects associated with CyA were observed. Thus, CyA treatment is useful for prevention of immune responses associated with the administration of human proteins in monkeys. 相似文献
37.
Watanabe O Ishii J Kitagaki T Okawa H Matsumoto H Kameyama M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(5):573-581
Establishment of a disposal plan for carcasses in advance is important for prevention of epidemics. A disposal plan for contaminated goods such as poultry carcasses infected with highly pathogenic avian influenza (HPAI) virus was studied in Hyogo Prefecture, Japan. We investigated all poultry farms with over 1,000 birds for their locations, species and numbers of birds, structure and size of poultry facilities and land spaces of the farms. Moreover, we judged whether they could dispose of all the carcasses at their farms. In 2005, 5.5 million layers and 2.7 million broilers were being kept. If HPAI had broken out, 44.0% of the farmers could bury all the carcasses, and 65.6% could compost them at their farms. However, 23.4% could not dispose of them except by burning them at incineration facilities. We decided to choose burning first for rapid disposal as long as the virus was not a pandemic type. 相似文献
38.
Norihito Yamauchi Hayato Horinouchi Kazuhiko Sakai Kengo Yonemoto Mamoru Satou Takashi Shirakawa 《Journal of General Plant Pathology》2011,77(4):260-262
The race of field isolates of Peronospora farinosa f. sp. spinaciae (Pfs), causal agent of spinach downy mildew, were identified using race-differential cultivars. One isolate was similar to
race Pfs:6. Three isolates were identified as race Pfs:8, the first time the race has been reported in Japan as far as we
know. The differential reaction caused by the other two isolates did not match any known to be caused by races Pfs:1 through
Pfs:11; thus, this strain appears to a new pathogenic strain in Japan. 相似文献
39.
40.
Boonchit S Alhassan A Chan B Xuan X Yokoyama N Ooshiro M Goff WL Waghela SD Wagner G Igarashi I 《Veterinary parasitology》2006,137(1-2):28-35
Recombinant antigen-based enzyme-linked immunosorbent assay (ELISA) was developed for the serological diagnosis of Babesia bigemina infection by using a full-length B. bigemina rhoptry-associated protein 1 (rRAP-1) and the truncated C-terminal RAP-1 (rRAP-1/CT). While the rRAP-1 showed cross reactivity between B. bigemina- and Babesia bovis-infected bovine sera, the rRAP-1/CT was highly specific to B. bigemina-infected bovine sera and proved useful in the detection of sequential sera collected from an experimentally infected cow during the acute and latent infection. The high yield of soluble rRAP-1/CT and its diagnostic specificity demonstrate its potential in the diagnosis of B. bigemina infection. Its usefulness for epidemiological investigation is currently being evaluated. 相似文献