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11.
Hikono H Miyazaki A Mase M Inoue M Hasegawa M Saito T 《Veterinary immunology and immunopathology》2012,146(1):92-96
Protecting pigs from simultaneous infection with avian, swine, and human influenza viruses would be an effective strategy to prevent the emergence of reassortants with pandemic potential. M2 protein is a candidate antigen for so-called 'universal vaccines,' which confer cross-protection to different influenza viruses in a strain- and subtype-independent manner. We tested whether a recombinant F gene-deleted Sendai virus vector that contained an M2 gene derived from an H5N1 avian influenza virus (SeV/ΔF/H5N1M2) could induce a cross-reactive antibody response to the extracellular domain of M2 protein (M2e) in pigs. SeV/ΔF/H5N1M2 induced an antibody response to M2e when the vector was inoculated intramuscularly. The antibodies induced by SeV/ΔF/H5N1M2 cross-reacted with M2e derived from different avian, swine, and human influenza viruses. In mice, however, SeV/ΔF/H5N1M2 did not confer cross-protection to challenge with a heterologous H3N2 influenza virus. Our results confirm those of other groups indicating that antibodies to M2e do not mediate protection to influenza viruses in pigs. 相似文献
12.
Tanabe T Shimoda M Soeno T Suzuki M Tajima M Sato H 《Veterinary immunology and immunopathology》2008,126(1-2):20-26
The interferon-stimulated gene 15 (ISG15) is induced by type I interferon (IFN). Recent studies have revealed that like ubiquitin, ISG15 is conjugated with target proteins. In this study, the feline ISG15 (FeISG15) gene was cloned from feline IFNomega (FeIFNomega)-stimulated feline kidney epithelial (CRFK) cells. According to gene sequence results, cDNA was 474bp long and encoded a protein of 157 amino acids. The putative amino acid sequences showed 62.5-72.1% identity with those of other mammalian ISG15s. Similar to human and mouse ISG15, FeISG15 included tandem ubiquitin-like domains; its homology with feline ubiquitin was 36.3-39.5%. The LRLRGG conjugating motif was located only in the carboxyl terminal ubiquitin-like domain. FeISG15 also lacked the carboxyl terminal extension after the LRLRGG motif, which is present in mouse and human ISG15. Recombinant FeISG15 protein was expressed as a His-tagged fusion protein in Escherichia coli and purified by ion-exchange chromatography followed by affinity chromatography. Monoclonal anti-FeISG15 antibodies revealed free FeISG15 and FeISG15 conjugated with target proteins in cells after IFNomega stimulation by Western blotting analysis. Furthermore, mRNA of IFNgamma was detected from peripheral blood mononuclear cells (PBMCs) after stimulation with rFeISG15 extracellularly by RT-PCR. Taken together, these results suggested that FeISG15 had ubiquitin- and cytokine-like activity, as in other species. 相似文献
13.
Tran Van Do Phung Dinh Trung Mamoru Yamamoto Osamu Kozan Nguyen Toan Thang Dang Van Thuyet 《Journal of Sustainable Forestry》2018,37(1):1-14
Forest ecosystems can modify the atmospheric CO2 through biomass accumulation mostly in tree stems with diameter at breast height (DBH) ≥ 10 cm. Aboveground biomass increment (ΔAGB), and changes in stand AGB, no. stems and basal area (BA) were calculated from mortality, recruitment, and growth data of tree stems in tropical evergreen broadleaved forest, Central Highland Vietnam. Data were derived from ten 1-ha permanent plots established in 2004, where all stems with DBH ≥ 10 cm were tagged, identified to species, and measured for DBH in 2004 and 2012. In an 8-year duration, the increment was 53 ± 10 stems ha–1, 7.8 ± 0.3 m2 ha–1 for BA and 86.0 ± 4.6 Mg ha–1 for AGB. The stem mortality rate was 0.9% year–1 and the stem recruitment rate was 2.2% year–1. Annual ΔAGB was 10.8 Mg ha–1 year–1, equaling to 5.4 Mg C ha–1 year–1. Of which, tree stems of 35–80 cm DBH classes accounted for 65%. The results indicated that the forest is in stage of carbon sequestration. Any disturbances causing death of 35–80 cm DBH tree stems will much reduce carbon sequestration capacity and it will take a long time for AGB to return to pre-disturbance stage. 相似文献
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15.
Mamoru Satou Yoshiaki Chikuo Yosuke Matsushita Katsuhiko Sumitomo 《Journal of General Plant Pathology》2010,76(3):225-228
Cutting rot of chrysanthemum was found on cuttings of cv. Jimba No.2 in 2008. The cuttings were imported, then transplanted
in Aichi Prefecture. Root development was not initiated in about 30% of the cuttings. The cut stem ends developed black discolouration
and decay. When healthy cuttings were the fungus isolated from diseased cuttings, these cuttings developed the same disease
symptoms. The characteristics and morphology of the fungal culture were identical to those of Plectosporium tabacinum. We propose that the new disease be named cutting rot of chrysanthemum. 相似文献
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17.
Polyhedra ofSpilosoma imparilis nucleopolyhedrovirus (SiNPV) were administered per os toSpilosoma imparilis (Lepidoptera: Arctiidae) larvae with and without the spindles of an entomopoxvirus fromAnomala cuprea (Coleoptera: Scarabaeidae). It was found that the addition of the spindles to the inoculum (the polyhedra) strongly enhanced
the infection of SiNPV in 5th and 6th instar larvae; the enhancement degree was estimated to be several thousand-fold in 6th
instar larvae. 相似文献
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19.
Tomohiro SASANAMI Mamoru OHTSUKI Yukio DOI Ryota TANAKA Atsushi FUJISHIMA Makoto MORI 《Animal Science Journal》2008,79(1):104-110
The extracellular matrix surrounding the oocyte before ovulation is called the perivitelline membrane (PL) in avian species. The PL is constructed with two major glycoproteins, ZPC and ZP1, which are synthesized in the ovarian granulosa cells and the liver, respectively. Although the properties of the major components in the PL have been examined, knowledge about the nature of its minor constituents is lacking. In this study we focused on PL protein, which migrates at 46‐kDa in the gel of SDS‐PAGE. N‐terminal sequence analysis demonstrated that the 46‐kDa protein is the C‐terminal fragment of ZP1. Analysis of lysylendopeptidase digests or cyanogens bromide‐degraded fragments of ZP1 confirmed this postulate. Western blot analysis using antiserum against 46‐kDa protein indicated the absence of 46‐kDa protein in the serum. Moreover, small immunoreactive bands, thought to be cleaved fragments of ZP1, were detected in the PL lysate by western blot analysis using antiserum against the N‐terminal peptide of ZP1. These results indicated that the N‐terminal proteolytic processing of ZP1 might take place after the arrival of ZP1 at the ovary, and the resulting product, 46‐kDa protein, is incorporated into the PL. 相似文献
20.
Kim JS Hinchcliff KW Yamaguchi M Beard LA Markert CD Devor ST 《Veterinary journal (London, England : 1997)》2005,169(3):397-403
The purpose of the present study was to determine the age-related changes in myosin heavy chain (MHC) composition and muscle oxidative and glycolytic capacity in 18 horses ranging in age from two to 30 years. Muscle samples were collected by excisional biopsy of the semimebranosus muscle. MHC expression and the key enzymatic activities were measured. There was no significant correlation between horse age and the proportions of type-IIA and type-IIX MHC isoforms. The percentage of type-I MHC isoforms decreased with advancing age. Muscle citrate synthase activity decreased, whereas lactate dehydrogenase activity increased with increasing age. Muscle 3-OH acyl CoA dehydrogenase activity did not change with ageing. The results suggest that, similar to humans, the oxidative capacity of equine skeletal muscle decreases with age. The age-related changes in muscle metabolic properties appear to be consistent with an age-related transition in MHC isoforms of equine skeletal muscle that shifts toward more glycolytic isoforms with age. 相似文献