Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: a collaborative trial

Diagnostic differentiation between the ruminant pathogens Mycoplasma agalactiae and Mycoplasma bovis is known to be problematic when only conventional serological and biochemical tests are used. The main reason for this is that both agents share a considerable number of related proteins and common epitopes. DNA-based detection methods offer advantages in terms of specificity and sensitivity. However, there is an urgent need to compare currently used PCR assays because they target different genomic regions and, therefore, may perform differently. In the present work, five laboratories, which use PCR routinely, evaluated the specificity of four different PCR systems for M. agalactiae and three systems for M. bovis on a total of 41 strains of the two Mycoplasma species including six previously unidentified strains. As the vast majority of PCR examinations (97.1% of all tests) correctly identified the strains the specificity of all seven detection systems appears to be high. In four cases, incorrect identification by conventional diagnostic methods was rectified by PCR. Isolates from non-typical hosts, i.e. three M. bovis strains from small ruminants and two M. agalactiae strains from cattle, were characterised by sequencing the 16S and part of the 23S ribosomal RNA genes.     

The role of the wood mechanical industry in the Swedish forest industry cluster

The forest and forest products form one of the most important basis for the transfer to a bio-based economy in Sweden. About 75% of the area covered by forest in Sweden is used industrially to produce raw material for the wood-refining industries. Every year, this cluster uses 75 million m³ of roundwood and has an export value of €12 billion. This review paper is devoted to the wood mechanical industry, i.e. the industry which turns the forest into sawn timber, packaging, construction wood, furniture and interior fittings. The sawmills consume about half of the volume of softwood which is felled, and about two-thirds of the sawn timber go to export without any further refining within the country. Nevertheless, in spite of the relatively low degree of refinement in the sawmill and the fact that the sawmills in general over time have a very low profitability, they are responsible for 70–80% of the forest owners' profits on the sale of timber. An increased upgrading of the sawn timber within the country is desirable from a national economic viewpoint – increased employment opportunities, increased export income, etc. It should then in the first place be for products with a higher added value, such as furniture and fittings. Today, the refinement value is 15–20 times higher for products from joinery and furniture industries compared to that of the sawn timber, and the added value of the wood within the building industry is only about 1.5 times.     

ZUR SELEKTIVITAT VON PHENMEDIPHAM ALS NACHAUFLAUFHERBIZID IN BETA-RÜBEN*

Summary. Sugar beet are outstandingly tolerant to 3–methoxycarbonyIaminophenyl-A′-(3′-methylphenyl) carbamate phenmedipham) and the difference in susceptibility between the main weeds prevalent in beet crops and the beet themselves can be used to obtain selective weed control. The herbicide is applied post-emergence; action via the soil can be excluded as non-existent. A test with twenty-nine varieties of sugar beet and one variety of fodder beet showed no interaction between phytotoxicity and variety. No variety particularly susceptible to phenmedipham was found. Investigations on the mode of action and selectivity proved that phenmedipham is a strong inhibitor of the Hill reaction. There were no differences in the extent of inhibition of the Hill reaction in chloroplasts of a sensitive plant (mustard) as compared with a resistant species (sugar beet). Measurements of photosynthesis using infra-red absorption methods and intact plants showed that it was inhibited in both sensitive and resistant species: the degree of inhibition, however, varied according to the species. In contrast to susceptible weeds, the resistant sugar beet and Amarantus relroflexus showed recovery in assimilation, and the recovery phases were significant. It can thus be concluded that sugar beet and A. retroftexus are able to inactivate phenmedipham. Sélectivité du phenmediphame, herbicide de post-levée dans les cultures de de betteraves sucrièes     

The effect of dietary vitamin E on the pleopodal egg number of Astacus leptodactylus (Eschscholtz, 1823)

The effect of vitamin E on the pleopodal egg number of Astacus leptodactylus (Eschscholtz, 1823) was studied. Crayfish were fed 2% of their total wet weight daily with vitamin E supplemented diets and a control for 70 days. The vitamin E content of the control diet, diet 1, diet 2 and diet 3 were 20 mg kg^–1, 40 mg kg^–1, 80 mg kg^–1 and 160 mg kg^–1 respectively on a dry weight basis. Vitamin E levels of the control and experimental diets were analysed by High Performance Liquid Chromatography. Results showed that diets containing supplemental vitamin E were associated with an increase in the number of pleopodal eggs. The best result was obtained with diet 2 containing 80 mg kg^–1 supplemental vitamin E.     

Masked,diluted and drowned out: how global seafood trade weakens signals from marine ecosystems

Nearly 40% of seafood is traded internationally and an even bigger proportion is affected by international trade, yet scholarship on marine fisheries has focused on global trends in stocks and catches, or on dynamics of individual fisheries, with limited attention to the link between individual fisheries, global trade and distant consumers. This paper examines the usefulness of fish price as a feedback signal to consumers about the state of fisheries and marine ecosystems. We suggest that the current nature of fisheries systems and global markets prevent transmission of such price signals from source fisheries to consumers. We propose several mechanisms that combine to weaken price signals, and present one example – the North Sea cod – to show how these mechanisms can be tested. The lack of a reliable price feedback to consumers represents a challenge for sustainable fisheries governance. We therefore propose three complimentary approaches to address the missing feedback: (i) strengthening information flow through improved traceability and visibility of individual fishers to consumers, (ii) capitalizing on the changing seafood trade structures and (iii) bypassing and complementing market mechanisms by directly targeting citizens and political actors regarding marine environmental issues through publicity and information campaigns. These strategies each have limitations and thus need to be pursued together to address the challenge of sustainability in global marine fisheries.     

Impact of origin and condition of host fish (Salmo trutta) on parasitic larvae of Margaritifera margaritifera

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Pancam multispectral imaging results from the Spirit Rover at Gusev Crater

Panoramic Camera images at Gusev crater reveal a rock-strewn surface interspersed with high- to moderate-albedo fine-grained deposits occurring in part as drifts or in small circular swales or hollows. Optically thick coatings of fine-grained ferric iron-rich dust dominate most bright soil and rock surfaces. Spectra of some darker rock surfaces and rock regions exposed by brushing or grinding show near-infrared spectral signatures consistent with the presence of mafic silicates such as pyroxene or olivine. Atmospheric observations show a steady decline in dust opacity during the mission, and astronomical observations captured solar transits by the martian moons, Phobos and Deimos, as well as a view of Earth from the martian surface.     

Selection for egg shell strength in laying hens using shell membrane characteristics

1. Divergent selection for attachment strength between the shell membrane and the calcium shell was performed in a White Leghorn strain. Multivariate analysis was used to estimate genetic parameters for shell membrane measurements and shell thickness. The aim was to investigate the possibility of improving shell strength in laying hens by selecting for increased attachment strength.

2. A significant direct selection response for attachment strength was achieved. There was a favourable correlated selection differential in the frequency of cracked eggs which resulted in a nonsignificant but favourable, correlated response. Selection for a strong attachment resulted in a thinner egg shell, and vice versa. Heritabilities were all relatively high (0.30 to 0.70). Most of the genetic correlations were in agreement with the achieved correlated responses.

3. Two factors that probably contributed to the responses in egg shell thickness were an unfavourable genetic correlation between attachment strength and shell thickness, and natural selection against changes in attachment strength, in the form of increased mortality during incubation and hatching. Problems connected with breeding for increased shell strength are discussed.