Fatty Acid Composition and Oxidative Stability of Fish Oil Products in Turkish Retail Market

The fatty acid composition and oxidative stability of fish oil and syrups available in the Turkish retail market were examined in this study. The major saturated fatty acids in capsules and syrups were palmitic acid (16:0) and stearic acid (18:0). The monounsaturated fatty acid contents of fish oil products have very different results (10.71–50.46%). The results show that the label claims for total omega-3, eicosapentaenoic acid, and docosahexaenoic acid presented a reasonable accuracy for the examined products, but it was noted that some of the results showed a considerable difference with the labels.

The free fatty acid level in fish oil products was generally low (0.13–1.95% of oleic acid). Peroxide value of all examined products was below the limit of 10 meq kg^?1 oils for edible oil as indicated in Codex (1999). It was detected that the p-Anisidine value of fish oil capsules (5.36–8.90) was considerably lower than for fish oil syrups (21.86–26.74). According to our results, the totox value of fish oil capsules evaluated in this study (7.08–17.35) was within acceptable limits. However, fish oil syrups (34.72–38.06) highly exceeded the upper tolerable limit (26).     

Note:Cnaemidophorus rhododactyla, a NewRosa spp. Pest for Turkey, and its new parasitoids, a NewRosa spp. Pest for Turkey, and its new parasitoids

In the present study,Cnaemidophorus rhododactyla (Denis & Schiffermüller) (Lepo Pterophoridae) was noted for the first time as a pest ofRosa spp. in Turkey. The larva ofC. rhododactyla makes a cavity by feeding in the flower-bud and destroys at least 60% of the mass of the flower-bud. There are approximately 12 species ofRosa in Erzurum and Kars provinces, butRosa dumalis Bechst. andR. subcanina L. suffered more damage than the otherRosa species. One species of Tachinidae (Diptera) —Pseudoperichaeta palesoidea (Robineau-Desvoidy), and two species of Ichneumonidae (Hymenoptera) —Sinophorus turionus (Ratz.) andScambus brevicornis Gravenhorst, were reared as larval parasitoids ofC. rhododactyla. C. rhododactyla is a new host record for these parasitoids. Additionally,S. turionus is a new record for the Turkish fauna.     

Recruitment of the threatened mussel Margaritifera margaritifera in relation to mussel population size,mussel density and host density

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Effects of Pressurized Low Polarity Water Extraction Parameters on Antioxidant Properties and Composition of Cow Cockle Seed Extracts

Antioxidant activity of pressurized low polarity water (PLPW) extracts of cow cockle seed and extraction residues were determined using DPPH, ABTS, and FRAP assays. The effect of extraction conditions (temperature (125, 150 and 175 °C) and time) on the antioxidant activity and the relationship amongst the antioxidant activity and extract composition (total phenolics and saponin content) were determined. The antioxidant activity of PLPW extracts increased with extraction temperature. Increasing activity with time was also observed at 175 °C. PLPW extraction residues had the highest activity suggesting antioxidant compounds were not completely extracted by PLPW. Antioxidant activity correlated well with total phenolics content of samples (R ² ≥ 0.94), however no correlation was observed with the saponin content. A strong correlation was observed between the antioxidant activity values obtained using different methods (R ² ≥ 0.94). These results point to the potential of PLPW extraction as a method to modify the activity of biological materials for the production of customized extracts.     

Present-day impact cratering rate and contemporary gully activity on Mars

The Mars Global Surveyor Mars Orbiter Camera has acquired data that establish the present-day impact cratering rate and document new deposits formed by downslope movement of material in mid-latitude gullies on Mars. Twenty impacts created craters 2 to 150 meters in diameter within an area of 21.5 x 10(6) square kilometers between May 1999 and March 2006. The values predicted by models that scale the lunar cratering rate to Mars are close to the observed rate, implying that surfaces devoid of craters are truly young and that as yet unrecognized processes of denudation must be operating. The new gully deposits, formed since August 1999, are light toned and exhibit attributes expected from emplacement aided by a fluid with the properties of liquid water: relatively long, extended, digitate distal and marginal branches, diversion around obstacles, and low relief. The observations suggest that liquid water flowed on the surface of Mars during the past decade.     

Differential gene expression of CYP3A isoforms in equine liver and intestines

Tydén, E., Löfgren, M., Pegolo, S., Capolongo, F., Tjälve, H., Larsson, P. Differential gene expression of CYP3A isoforms in equine liver and intestines. J. vet. Pharmacol. Therap.  35 , 588–595. Recently, seven CYP3A isoforms – CYP3A89, CYP3A93, CYP3A94, CYP3A95, CYP3A96, CYP3A97 and CYP129 – have been isolated from the horse genome. In this study, we have examined the hepatic and intestinal gene expression of these CYP3A isoforms using TaqMan probes. We have also studied the enzyme activity using luciferin‐isopropyl acetal (LIPA) as a substrate. The results show a differential gene expression of the CYP3A isoforms in the liver and intestines in horses. In the liver, CYP3A89, CYP3A94, CYP3A96 and CYP3A97 were highly expressed, while in the intestine there were only two dominating isoforms, CYP3A93 and CYP3A96. The isoform CYP3A129 was not detected in the liver or the intestine, although this gene consists of a complete set of exons and should therefore code for a functional protein. It is possible that this gene is expressed in tissues other than the liver and intestines. In the intestine, both CYP3A96 and CYP3A93 showed the highest gene expression in the duodenum and the proximal parts of the jejunum. This correlated with a high protein expression in these tissues. Studies of the enzyme activity showed the same K_m for the LIPA substrate in the liver and the intestine, while the maximum velocity (V_max) in the liver was higher than in the intestine. Our finding of a differential gene expression of the CYP3A isoforms in the liver and the intestines contributes to a better understanding of drug metabolism in horses.     

The effect of different nitrogen levels on yield and quality of stevia (Stevia rebaudiana bert.)

In the present work, the efficiency of different nitrogen doses (0, 50, 100, 150, and 200 kg ha^?1) on growth, yield, and quality of stevia (Stevia rebaudiana Bert.) was investigated in 2011–2013. The study was conducted in Antalya located in the Mediterranean Region of Turkey. Terra rossa type soil (LVx, FAO) characteristics of the experimental field were clay loam, with high amounts of lime (33,9%) and slightly alkaline (pH 7.7). The experiment was carried out in randomized block design with four replications. All the results were summarized as mean of three years. The highest fresh and dry biomass yields (26.75 t ha^?1 and 7.5 ha^?1, respectively) were obtained from 150 kg ha^?1 N dose and followed by 100 kg ha^?1 N dose (26.29 t ha^?1 and 7.24 ha^?1, respectively). Whereas the highest fresh and dry leaf yields (13.27 t ha^?1 and 3.82 t ha^?1, respectively) were realized in 100 kg ha^?1 N dose. Actually, all nitrogen doses gave higher biomass and leaf yields compared to the control. On the hand, major steviol glycosides (stevioside and rebaudioside A) in the leaf were not influenced by nitrogen levels. In conclusion, 100 kg ha^?1 N dose was found to be suitable for cultivation of stevia under field conditions.     

Alleviation of salt stress by increasing potassium sulphate doses in four medicinal and aromatic plants

In this study, the effects of potassium doses (control, 150, 300 and 450?mg K₂SO₄ kg^?1) and salt stress (control, 100?mM NaCl) on the yield and some element content of four medicinal and aromatic (Coriandrum sativum, Anethum graveolens, Ocimum basilicum and Foeniculum vulgare) plants were investigated in climate chamber. Both salinity and K fertiliser levels affected the fresh and dry weight of all evaluated plants. Anethum graveolens, Ocimum basilicum species are more sensitive to salinity, particularly at the vegetative productive stages. The highest fresh and dry weights of leaves, stems, roots and herb in Coriandrum sativum and Foeniculum vulgare species was observed in 300?mg?kg^?1 fertiliser applications. In general, there was a relatively consistent and positive correlation between root element content and aerial parts element content. The result of the present study showed that NaCl treatment caused an increase in Na⁺ concentration, and a decrease in K⁺ and Ca⁺² concentration in Coriandrum sativum, Anethum graveolens, Ocimum basilicum and Foeniculum vulgare. There was an interaction between K₂SO₄ application and salinity effects on Na⁺ concentration in the all evaluated plants. Given the experimental results, especially Foeniculum vulgare and Coriandrum sativum species were the most resistant to salt stress.     

Heavy loads of parasitic freshwater pearl mussel (Margaritifera margaritifera L.) larvae impair foraging,activity and dominance performance in juvenile brown trout (Salmo trutta L.)

The life cycle of the endangered freshwater pearl mussel (Margaritifera margaritifera) includes a parasitic larval phase (glochidia) on the gills of a salmonid host. Glochidia encystment has been shown to affect both swimming ability and prey capture success of brown trout (Salmo trutta), which suggests possible fitness consequences for host fish. To further investigate the relationship between glochidia encystment and behavioural parameters in brown trout, pairs (n = 14) of wild‐caught trout (infested vs. uninfested) were allowed to drift feed in large stream aquaria and foraging success, activity, agonistic behaviour and fish coloration were observed. No differences were found between infested and uninfested fish except for in coloration, where infested fish were significantly darker than uninfested fish. Glochidia load per fish varied from one to several hundred glochidia, however, and high loads had significant effects on foraging, activity and behaviour. Trout with high glochidia loads captured less prey, were less active and showed more subordinate behaviour than did fish with lower loads. Heavy glochidia loads therefore may negatively influence host fitness due to reduced competitive ability. These findings have implications not only for management of mussel populations in the streams, but also for captive breeding programmes which perhaps should avoid high infestation rates. Thus, low levels of infestation on host fish which do not affect trout behaviour but maintains mussel populations may be optimal in these cases.