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排序方式: 共有904条查询结果,搜索用时 15 毫秒
31.
Tamura K Arai H Ueno E Saito C Yagihara H Isotani M Ono K Washizu T Bonkobara M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(9):925-930
Dendritic cell (DC) vaccination is one of the most attractive immunotherapies for malignancies in dogs. To examine the differences in DC-mediated immune responses from different types of malignancies in dogs, we vaccinated dogs using autologous DCs pulsed with keyhole limpet hemocyanin (KLH) and cell lysate prepared from squamous cell carcinoma SCC2/88 (SCC-KLH-DC), histiocytic sarcoma CHS-5 (CHS-KLH-DC), or B cell leukemia GL-1 (GL-KLH-DC) in vitro. In vivo inductions of immune responses against these tumor cells were compared by the delayed-type hypersensitivity (DTH) skin test. The DTH response against SCC2/88 cells were observed in dogs vaccinated with autologous SCC-KLH-DC, while the response was undetectable against CHS-5 and GL-1 cells in dogs vaccinated with autologous CHS-KLH-DC and GL-KLH-DC. Skin biopsies taken from DTH challenge sites were then examined for immunohistochemistry, and recruitment of CD8 and CD4 T cells was detected at the site where SCC2/88 cells were inoculated in dogs vaccinated with SCC-KLH-DC. By contrast, neither CD8 nor CD4 T cell infiltration was found at the DTH challenge site in the dogs vaccinated with CHS-KLH-DC or GL-KLH-DC. These findings may reflect that the efficacy of immune induction by DC vaccination varies among tumor types and that immune responses could be inducible in squamous cell carcinoma. Our results encouraged further investigation of therapeutic vaccination for dogs with advanced squamous cell carcinoma in clinical trials. 相似文献
32.
Azakami D Bonkobara M Washizu T Iida A Kondo M Kato R Niikura Y Iwaki S Tamahara S Matsuki N Ono K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(12):1343-1346
Seven novel cell lines from canine histiocytic sarcoma (HS), three of which were disseminated cutaneous HS and four of which were synovial HS, were established. All of the established cell lines had the same morphological (by light and electron microscopic findings), cytochemical (alpha-naphthyl butyrate esterase-positive), and immunohistochemical (vimentin- and lysozyme-positive, and cyto-keratin-negative) characteristics as the original HS tumor cells. All of the established cell lines injected into nude mice subcutaneously produced solid tumors. Because the established cell lines also showed phagocytic and processing activities, the HS tumor cells appear to originate from the mononuclear phagocytic system cells, despite their differences in locations or organs. 相似文献
33.
Matsuura Y Suzuki M Hayakawa D Asano M Sasaki M Kitamura N Yamada J Tsubota T Ohtaishi N 《The Japanese journal of veterinary research》2004,51(3-4):167-172
We analyzed the localization of steroidogenic enzymes (P450 scc, 3 beta HSD, P450 arom and P450 c17) in the corpora lutea of two Hokkaido sika deer (Cervus nippon yesoensis) during the early mating season. Two corpora lutea were found in each female and the timing of formation of the corpora lutea seemed different. P450 scc, and 3 beta HSD, positive luteal cells were found in both corpora lutea. The existence of two functional corpora lutea from the early mating season through pregnancy suggests that progesterone secreted by two or more corpora lutea is necessary for maintenance of pregnancy in sika deer. 相似文献
34.
Makoto ANDO 《中国林学(英文版)》2012,14(2):85-91
We studied the impact of stand density and forest floor humus removal on regeneration of Pinus densiflora in a mixed conifer and broad-leaved forest on the Daimonji-Yama Mountain,Kyoto,Japan.Three levels of stand density were set,i.e.,a clear-cut site,a heavily thinned site and a lightly thinned site.In each density treatment,comparisons were carried out between humus removal (A 0 -free)and humus intact(A 0 -intact)subplots.We counted the emergence of sown pine seeds and measured survival and growth of pine seedlings over the next four years.In addition,light conditions and the recovery of understory were monitored.Results show that thinning intensity and humus removal promoted the establishment and growth of seedlings.One exception,however,was that seedling growth was minimal in the heavily thinned A 0 -intact subplots.This was due to a strong response of understory vegetation growth on the treatment combination,which hindered the pine seedling growth.Furthermore,we found that the canopy openness measured at 1.3-m height remained at least 35%for the heavily thinned site in 2008.The results suggest that the removal of the A 0 layer after heavy thinning is the most effective and labor-saving operation for P.densiflora regeneration. 相似文献
35.
Makoto Kiguchi Yutaka Kataoka Hiroshi Matsunaga Koichi Yamamoto Philip D. Evans 《Journal of Wood Science》2007,53(3):234-238
The market for wood-fiber plastic composites (WPCs) is expanding rapidly in many countries including Japan, where WPCs are
mainly used for exterior products. In such applications, WPCs undergo undesirable color change, chalking, and shrinkage and
swelling, and accordingly there is a need to better understand the mechanisms responsible for the weathering of WPC and develop
methods of improving their weathering resistance. In this study, weatherability of WPC was assessed by natural and accelerated
weathering trials. Discoloration (whitening) of WPC during exposure was caused by degradation of both wood and plastic. Darker
color pigments as additives improved the color stability of WPC; however, chalking on the surfaces still occurred. The color
stability of WPC was improved by application of exterior coatings. Preweathering of WPC (before coatings were applied) increased
the absorption of coatings by the WPC and had a positive effect on the color stability and prevented chalking of the composites.
Parts of this study were presented at the IUFRO XXII World Congress Meeting, Brisbane, August 2005 and the IAWPS 2005, Yokohama,
November 2005 相似文献
36.
Makoto?Watanabe Masahiro?Yamaguchi Hideyuki?Matsumura Yoshihisa?Kohno Takeshi?IzutaEmail author 《European Journal of Forest Research》2012,131(2):475-484
Tropospheric ozone (O3) is considered to be the air pollutant relating to the decline of Fagus crenata forest in Japan. In the present study, we assessed a risk of O3 impact on the growth of F. crenata in Japan, giving consideration to the effects associated with atmospheric nitrogen (N) deposition based on the experimental
study, national monitoring data for oxidant concentration and atmospheric N deposition, and a national vegetation survey.
The average and maximum O3-induced relative growth reduction (RGred) of F. crenata across Japan were estimated to be 3.2 and 9.7%, respectively. Current levels of atmospheric N deposition were found to significantly
affect the sensitivity of F. crenata to O3. When the N deposition was assumed as zero, the estimated average and maximum RGred were 2.3% and 5.7%, respectively. The inclusion of atmospheric N deposition data thus increased the estimated values for
average and maximum RGred (by 38% and 71%, respectively). Our results demonstrate that a change in the sensitivity to O3 associated with atmospheric N deposition is an important consideration in the risk assessment of O3 impact on the growth of F. crenata in Japan. 相似文献
37.
Dietary intake of iodine‐enriched eggs decreases the incidence of mouse mammary tumors caused by the activated ErbB2 oncogene 下载免费PDF全文
Kazunori Oyama Takahiro Shimoda Makoto Miyagawa Mizuki Sone Jiro Yokoyama Katsuhiko Nishimori Tomokazu Fukuda 《Animal Science Journal》2018,89(8):1169-1177
Human epigenetic studies suggest that consumption of seaweed prevents mammary cancer, which possibly is explained by iodine daily intake. In this study, we evaluated the efficacy of dietary intake of iodine‐enriched eggs on mammary tumor incidence caused by the expression of activated type ErbB2. Female transgenic mice were divided into three groups, and fed a basic diet, a diet supplemented with ordinary eggs, or with iodine‐enriched eggs. The number of mammary tumors greater than 5 mm in diameter was recorded in mice at 6 months of age. We report that the average number of mammary tumors per mouse was significantly lower in the iodine‐enriched egg‐added diet group than in either the basic diet or ordinary egg diet groups. These results indicate that iodine intake through livestock‐derived products can reduce the incidence of mammary cancers caused by the expression of activated type ErbB2. 相似文献
38.
Iimure T Kimura T Araki S Kihara M Sato M Yamada S Shigyou T Sato K 《Journal of agricultural and food chemistry》2012,60(6):1548-1554
Beer foam stability is an important characteristic. It has been suggested that isoforms of protein Z, that is, protein Z4 and protein Z7, contribute to beer foam stability. We investigated the relationship between beer foam stability and protein Z4 and protein Z7 using their deficient mutants. As a protein Z4-deficient mutant, cv. Pirkka was used. Protein Z7 deficiency was screened in 1564 barley accessions in the world collection of Okayama University, Japan. The barley samples from normal, protein Z4-deficient, protein Z7-deficient, and double-deficient were genotyped in F(2) populations and then pooled based on the DNA marker genotypes of protein Z4 and protein Z7. For a brewing trial, F(5) pooled subpopulations were used. After malting and brewing, the foam stability was determined, and the results showed that the levels of foam stability in the four samples were comparable. Two-dimensional gel electrophoresis was used to investigate the proteome in these beer samples. The results showed that low molecular weight proteins, including lipid transfer protein (LTP2), in the deficient mutants were higher than those in the normal sample. Our results suggest that the contribution of protein Z4 and protein Z7 to beer foam stability was not greater than that of other beer proteins. 相似文献
39.
Masashi?Hatamoto Takanori?Tanahashi Jun?Murase Kazuo?Matsuya Motoki?Hayashi Makoto?Kimura Susumu?AsakawaEmail author 《Biology and Fertility of Soils》2008,44(3):527-532
To estimate the succession and phylogenetic composition of the eukaryotic communities responsible for the decomposition of
rice straw compost under flooded conditions during the cultivation period of paddy rice, denaturing gradient gel electrophoresis
(DGGE) analysis targeting 18S rDNA followed by sequencing was conducted in a Japanese paddy field. The eukaryotic communities
in rice straw compost incorporated into the flooded paddy field were influenced by the mid-season drainage and mainly composed
of fungi (Ascomycota, Zygomycota, and Chytridiomycota) and protozoa (Ciliophora, Euglyphida, and Dactylopodida), most of which
existed continuously during the cultivation period of paddy rice. The results indicated that these eukaryotic members were
associated with the decomposition of rice straw compost in paddy field soil directly or indirectly. 相似文献
40.