Aurasperone A Inhibits SARS CoV-2 In Vitro: An Integrated In Vitro and In Silico Study

Several natural products recovered from a marine-derived Aspergillus niger were tested for their inhibitory activity against SARS CoV-2 in vitro. Aurasperone A (3) was found to inhibit SARS CoV-2 efficiently (IC₅₀ = 12.25 µM) with comparable activity with the positive control remdesivir (IC₅₀ = 10.11 µM). Aurasperone A exerted minimal cytotoxicity on Vero E6 cells (CC₅₀ = 32.36 mM, SI = 2641.5) and it was found to be much safer than remdesivir (CC₅₀ = 415.22 µM, SI = 41.07). To putatively highlight its molecular target, aurasperone A was subjected to molecular docking against several key-viral protein targets followed by a series of molecular dynamics-based in silico experiments that suggested M^pro to be its primary viral protein target. More potent anti-SARS CoV-2 M^pro inhibitors can be developed according to our findings presented in the present investigation.     

武汉地区三角梅遮光催花试验研究

本试验以1～3 a生的三角梅盆花为试材,以遮光处理为研究手段进行武汉地区三角梅花期调控试验探索,结果表明:在20～30℃的温度条件下,用栽培地布对三角梅进行连续遮光处理20 d,能完成稳定的花芽分化,继续遮光25 d可开花,并在此期间将腐熟的有机肥上清液稀释10倍灌根代替浇水能有效促进三角梅开花。     

体细胞数对发酵乳品质的影响研究

为确定用于生产发酵乳的原料乳质量标准,以提高发酵乳产品品质,选用蒙牛自有牧场30头不同健康状况、不同泌乳期的荷斯坦牛原料乳,按体细胞数分为低于20万个/mL组、20万-80万个/mL组及大于80万个/mL组,在发酵乳制备的当天及样品冷藏后的第1、7、14、21天分别测定发酵的产酸曲线、发酵乳的质构特性。结果表明,随着原料乳中体细胞数的增加,产品黏度下降,弹性降低,硬度增加,pH值降低,品质下降。     

京西山地棕壤和淋溶褐土饱和导水率的分析

导水率Ｋ（ｈｙｄｒａｕｌｉｃｃｏｎｄｕｃｔｉｖｉｔｙ）是表示孔隙介质透水性能的综合比例系数,即单位水力势梯度下水的通量密度或渗透流速。Ｋ值是土壤颗粒大小分布、固体物质的化学组成和土壤紧实度的函数。因此,对于不同的土壤或同一土壤的不同层次Ｋ值亦可能有差...     

三亚市10年间景观格局变化分析

为了充分了解热带滨海城市发展过程中景观变化特征及其影响因素,利用基于SPOT的影像数据,应用RS、GIS 软件和景观生态学方法,以海南省三亚市作为研究对象,分析了2002—2012 年三亚市土地利用及景观格局的动态变化情况。结果表明：（1）三亚市仍以耕地和阔叶林为主,建设用地大幅度增加,各土地利用类型有相互转移表现,2002—2012 年,水田面积减少了25.75%,水库/坑塘减少6.21%;草丛、建设用地和旱地面积分别增加143.16%、86.89%和10.20%;（2）运用景观格局指数和转移矩阵分析,得出研究区域景观斑块总体数量减少,说明这期间三亚景观破碎化趋势降低,主要由于人类活动的干扰不断加强,城市化进程加快使原来分散的乡镇村落等居民集中地聚集成片,转变为城市化建筑群,从而导致了研究区域景观破碎化程度的下降。     

刈割对龙葵生长和富集镉的影响及其机理

通过盆栽实验,研究了25mg·kg-1镉胁迫下刈割对超富集植物龙葵(Solanum nigrum L.)再生长能力和富集镉效果的影响。结果表明留茬30cm轻度刈割,新芽再生速度较快,留茬15cm深度刈割处理的再生速度则相对较慢,二者差异显著,但相对于对照来说刈割处理都能显著提高龙葵的生物量和生长速率。通过对不同时期龙葵体内的镉积累量的比较,发现刈割能有效提高龙葵富集镉的能力。刈割是提高龙葵对镉污染土壤修复效率的一种策略。     

On the Analysis of EMCS and Its Application in the Project of Shenzhen Underground

In this paper, the selection of type, function and technological requirements of EMCS for Shenzhen Underground has been introduced.Based on the investigation for EMCS's worldwide applications and the analysis of practice in Shenzhen Underground, the comparison and selection of PLC and DDC have been done completely.At the same time,the introduction of EMCS is organized by the center level, station level and the spot level.     

Identification and characterization of <Emphasis Type="Italic">Choanephora</Emphasis> spp. causing Choanephora flower rot on <Emphasis Type="Italic">Hibiscus syriacus</Emphasis>

Hibiscus syriacus, as a national flower of Korea, is most popularly used for ornamental purposes and includes numerous cultivars, and it is widely planted in temperate zones that feature hot summers. We investigated Choanephora flower rot on H. syriacus from 2012 to 2014 in Korea and Japan and confirmed Choanephora infection in several localities in both countries. Here, our objectives were to identify the main causal agent of Choanephora flower rot on H. syriacus and describe its morphological and molecular characteristics. We identified 44 out of 50 isolates as Choanephora cucurbitarum and the remainder as C. infundibulifera based on morphological characterization and phylogenetic analysis. The sequences of the internal transcribed spacer region (ITS) of ribosomal DNA and the D1/D2 region of the large subunit (LSU) rDNA of examined isolates were compared with sequences obtained from GenBank, and the analysis of the results revealed 100 % identity with the corresponding sequences of C. cucurbitarum and C. infundibulifera strains. Classification of the Choanephora species performed here according to the key described by Kirk (1984) corresponded with the results of the phylogenetic analysis of this study. Through intraspecific and interspecific mating tests, the characteristics of zygospore were described in details. Pathogenicity tests using both species showed the same symptoms, causing blossom blight and soft rot on the flowers, which were identical to those observed in the field. All identified causal agents of Choanephora rot were indeed Choanephora species, where C. cucurbitarum was identified in the majority, while the others were in the minority of examined samples.     

耐旱灌木蒙古莸花的资源及保护利用

蒙古莸是分布在黄土区和干旱的荒漠区,以及沙区的一种耐旱性小灌木,不仅抗性强,而且繁殖更新容易。它是一种花型优美、叶和种子含樟香型油。是很好的水土保持和环境绿化、美化树种,并且又是良好的“木本饲料”。今后对蒙古莸的保护、开发、利用前景广阔     

Detection and molecular characterization of sugarcane grassy shoot phytoplasma in Vietnam

Sugarcane is an important cash crop in Vietnam and has been widely promoted at national and provincial level. In 2006, a new disease was discovered in sugarcane in the Nghean Tate&Lyle Sugar Mill in Nghean Province in north-central Vietnam. The key symptoms were the formation of green grassy shoots around the base of mature stools. We applied nested polymerase chain reaction (PCR) using P1/P7 and R16F2n/R16R2 for detection and characterization of phytoplasma from the symptomatic tissues. PCR products of the expected size (approx. 1200?bp) were obtained from the 16S rDNA of the phytoplasma. The restriction fragment length polymorphism profiles indicated that all samples were infected by the same phytoplasma. Phylogenetic analysis confirmed that the SCGS phytoplasma from Vietnam belong to the 16SrXI group, formerly Rice Yellow Dwarf group.