Detection and molecular characterization of sugarcane grassy shoot phytoplasma in Vietnam

Sugarcane is an important cash crop in Vietnam and has been widely promoted at national and provincial level. In 2006, a new disease was discovered in sugarcane in the Nghean Tate&Lyle Sugar Mill in Nghean Province in north-central Vietnam. The key symptoms were the formation of green grassy shoots around the base of mature stools. We applied nested polymerase chain reaction (PCR) using P1/P7 and R16F2n/R16R2 for detection and characterization of phytoplasma from the symptomatic tissues. PCR products of the expected size (approx. 1200?bp) were obtained from the 16S rDNA of the phytoplasma. The restriction fragment length polymorphism profiles indicated that all samples were infected by the same phytoplasma. Phylogenetic analysis confirmed that the SCGS phytoplasma from Vietnam belong to the 16SrXI group, formerly Rice Yellow Dwarf group.     

武汉地区三角梅遮光催花试验研究

本试验以1～3 a生的三角梅盆花为试材,以遮光处理为研究手段进行武汉地区三角梅花期调控试验探索,结果表明:在20～30℃的温度条件下,用栽培地布对三角梅进行连续遮光处理20 d,能完成稳定的花芽分化,继续遮光25 d可开花,并在此期间将腐熟的有机肥上清液稀释10倍灌根代替浇水能有效促进三角梅开花。     

浅谈中国园林中的水榭

通过若干实例分析,探讨了中国园林中水榭的形式风格以及在园林中的功能。     

Solid-Phase Extraction Embedded Dialysis (SPEED), an Innovative Procedure for the Investigation of Microbial Specialized Metabolites

Solid-phase extraction embedded dialysis (SPEED technology) is an innovative procedure developed to physically separate in-situ, during the cultivation, the mycelium of filament forming microorganisms, such as actinomycetes and fungi, and the XAD-16 resin used to trap the secreted specialized metabolites. SPEED consists of an external nylon cloth and an internal dialysis tube containing the XAD resin. The dialysis barrier selects the molecular weight of the trapped compounds, and prevents the aggregation of biomass or macromolecules on the XAD beads. The external nylon promotes the formation of a microbial biofilm, making SPEED a biofilm supported cultivation process. SPEED technology was applied to the marine Streptomyces albidoflavus 19-S21, isolated from a core of a submerged Kopara sampled at 20 m from the border of a saltwater pond. The chemical space of this strain was investigated effectively using a dereplication strategy based on molecular networking and in-depth chemical analysis. The results highlight the impact of culture support on the molecular profile of Streptomyces albidoflavus 19-S21 secondary metabolites.     

利用手工克隆技术培育体细胞克隆莱芜猪

为探讨手工克隆技术在克隆中国地方猪种的可行性,本研究组利用手工克隆技术平台,以中国地方猪品种莱芜猪的耳皮肤细胞作为核移植的供体细胞,以体外成熟的猪卵母细胞为受体细胞,进行体细胞克隆猪培养,成功培育了25头克隆莱芜猪。表明手工克隆技术可应用于莱芜猪体细胞克隆,为莱芜猪的保种及育种提供了新的途径。     

小麦苗期根系性状的全基因组关联分析与优异位点挖掘

【目的】植物根系对水分及营养的获取、作物的生长发育和产量的形成至关重要。挖掘小麦苗期根系性状显著关联的SNP位点,预测相关候选基因,为解析小麦根系建成遗传机制及选育具有优良根系构型的小麦品种奠定基础。【方法】以189份小麦品种组成的自然群体为供试材料,调查2种培养条件（霍格兰营养液和去离子水）下培育21 d的苗期根系总长度（TRL）、根系总表面积（TRA）、根系总体积（TRV）、根系平均直径（ARD）及根系干重（RDW）等5个根系性状,试验进行2次重复,同时结合小麦660K SNP芯片的分型结果进行全基因组关联分析（genome-wide association study,GWAS）。此外,通过序列比对、结构域分析和注释信息预测候选基因,并采用竞争性等位基因特异性PCR(kompetitive allele specific PCR,KASP)技术开发根系性状的分子标记。【结果】霍格兰营养液培养条件下的根系性状变异范围较大,根系整体粗短;而去离子水条件下的根系细长、侧根较多。选用贝叶斯信息与连锁不平衡迭代嵌套式模型（BLINK）、压缩式混合线性模型（CMLM）、固定随机循环概率模型（...     

《花木栽培技术》的教学改革策略探寻

《花木栽培技术》是一门具有很强理论性和实践性的基础课程。现阶段《花木栽培技 术》课程教学中存在许多问题,在“宽专业、厚基础、重应用”的教育改革方向下,如何对《花木栽 培技术》教学进行科学、合理、有效的改革,提高《花木栽培技术》的教学质量和教学水平,值得一 线教育工作者进行深刻思考。本文以《花木栽培技术》教学中的问题为切入点,进一步探讨《花 木栽培技术》的教学改革策略。     

“蜜脆”食荚豌豆的选育及应用研究

食荚豌豆多以扁荚、高杆为主。课题组自1986年起经过6年努力有成了矮秆、早熟、适合多季栽培的圆棍型食荚豌豆新品种——蜜脆,并已在全国12个省市推广应用。     

耐旱灌木蒙古莸花的资源及保护利用

蒙古莸是分布在黄土区和干旱的荒漠区,以及沙区的一种耐旱性小灌木,不仅抗性强,而且繁殖更新容易。它是一种花型优美、叶和种子含樟香型油。是很好的水土保持和环境绿化、美化树种,并且又是良好的“木本饲料”。今后对蒙古莸的保护、开发、利用前景广阔