全文获取类型
收费全文 | 349篇 |
免费 | 42篇 |
专业分类
林业 | 29篇 |
农学 | 20篇 |
基础科学 | 1篇 |
52篇 | |
综合类 | 13篇 |
农作物 | 27篇 |
水产渔业 | 40篇 |
畜牧兽医 | 164篇 |
园艺 | 6篇 |
植物保护 | 39篇 |
出版年
2023年 | 3篇 |
2022年 | 14篇 |
2021年 | 20篇 |
2020年 | 17篇 |
2019年 | 27篇 |
2018年 | 16篇 |
2017年 | 16篇 |
2016年 | 21篇 |
2015年 | 16篇 |
2014年 | 18篇 |
2013年 | 21篇 |
2012年 | 27篇 |
2011年 | 31篇 |
2010年 | 15篇 |
2009年 | 14篇 |
2008年 | 14篇 |
2007年 | 15篇 |
2006年 | 12篇 |
2005年 | 10篇 |
2004年 | 12篇 |
2003年 | 9篇 |
2002年 | 9篇 |
2001年 | 6篇 |
2000年 | 1篇 |
1999年 | 3篇 |
1998年 | 5篇 |
1997年 | 2篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1984年 | 2篇 |
1980年 | 1篇 |
1969年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有391条查询结果,搜索用时 15 毫秒
361.
Botelho-Júnior S Siqueira-Júnior CL Jardim BC Machado OL Neves-Ferreira AG Perales J Jacinto T 《Journal of agricultural and food chemistry》2008,56(20):9404-9409
This work investigates the effect of methyl jasmonte (MeJa), mechanical wounding, and herbivory caused by larval feeding of a specialist insect ( Agraulis vanillae vanillae) upon trypsin inhibitory activity in passion fruit leaves. Despite the fact that all treatments caused accumulation of trypsin inhibitors (TIs), higher levels were observed in MeJa treated leaves when plants were assayed 24 and 48 h after stimulus. Concerning both mechanically injured plants and attacked ones, a systemic induction was observed. Partially purified inhibitors from MeJa exposed plants were further characterized by X-ray film contact print technique and N-terminal sequence. Such analysis indicated that the TIs identified belong to the Kunitz family. Moreover, the partially purified inhibitors strongly inhibited trypsin-like digestive enzymes from sugar cane stalk borer ( Diatraea saccharalis) in vitro. Our results further support the protective function of wound-inducible trypsin inhibitors and their potential as tools to improve important crop species against insect predation through genetic engineering. 相似文献
362.
Ramos Silvio Junio Caldeira Cecílio Frois Gastauer Markus Costa Deborah Luciany Pires Furtini Neto Antonio Eduardo de Souza Filipe Bittencourt Machado Souza-Filho Pedro Walfir Martins Siqueira Jose Oswaldo 《New Forests》2019,50(5):859-872
New Forests - For the effective use of native plants for mineland revegetation, an understanding of seed dormancy break and germination requirements, and seed storage tolerance is indispensable. In... 相似文献
363.
Márcia Silva Helena Machado Alan J. L. Phillips 《European journal of plant pathology / European Foundation for Plant Pathology》2009,125(3):425-433
Mycosphaerella leaf disease (MLD) is caused by species of Mycosphaerella and several anamorphic form genera that have been connected to Mycosphaerella. Until recently, MLD of eucalypts was largely ignored in Portugal. However, serious damage to Eucalyptus globulus has been reported since 1999 when frequent and severe defoliation of young trees was observed. The severity of this disease
prompted a preliminary study of the Mycosphaerella species associated with major symptoms of a leaf blotch disease in commercial plantations of E. globulus in Portugal, which is presented here. The species were identified by molecular methods based on the ITS1-5.8S-ITS2 cluster,
together with morphological characters. In addition to confirming the species previously recorded, Mycosphaerella vespa is reported for the first time from Portugal, while the status of Mycosphaerella grandis remains to be resolved. 相似文献
364.
K. S. Kubo J. Freitas-Astúa M. A. Machado E. W. Kitajima 《Journal of General Plant Pathology》2009,75(3):250-255
Brevipalpus-transmitted viruses (BTV) cause chlorotic, necrotic and/or ringspot lesions in leaves and stems of orchids, citrus, coffee
and several other plant species. There are two different types of BTVs, the nuclear and the cytoplasmic, based on maturation
locale in the cell and particle morphology. The orchid fleck virus (OFV) is a BTV that infects orchids. Its short rodlike
particles are 32–40 nm in diameter, 100–150 nm in length. OFV is found in the nucleus and is associated with intranuclear
electronlucent viroplasms. In 1999, transmission electron microscopy analysis revealed a distinct type of virus causing orchid
fleck symptoms. The bacilliform particles, 70–80 nm in diameter and 110–120 nm in length, induced electron-dense viroplasm
inclusions in infected cells and resembled the cytoplasmic type associated with BTV, such as the citrus leprosis virus C.
Our objective in the present study was to verify whether the cytoplasmic type virus found in orchids could be amplified using
primers for other cytoplasmic BTVs, such as CiLV-C and Solanum
violaefolium ringspot virus (SvRSV). Additionally, we aimed to differentiate the two BTVs found in orchids: the nuclear and the cytoplasmic
types of OFV using microscopy and molecular and serological tools. This virus was not amplified by the CiLV-C and SvRSV primers,
and neither the molecular nor the serological tools available to the OFV diagnosis reacted with it, demonstrating that they
are definitely different viruses. 相似文献
365.
Dorothy D. Whelchel DVM MS DACVIM Brett S. Tennent‐Brown BVSc MS DACVIM DACVECC Amanda E. Coleman DVM DACVIM Gregg S. Rapoport DVM DACVIM Uriel Blas‐Machado DVM PhD DACVP Herbert W. Maisenbacher VMD DACVIM Brenton C. Credille DVM PhD DACVIM Steeve Giguère DVM PhD DACVIM 《Journal of Veterinary Emergency and Critical Care》2017,27(3):362-368
366.
367.
368.
Laurie M. Millward Alex Hamberg Jennifer Mathews Cecilia Machado‐Parrula Christopher Premanandan Samuel D. A. Hurcombe M. Judith Radin Maxey L. Wellman 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2010,39(3):365-370
Abstract: A 6‐year‐old female Rocky Mountain horse was presented for evaluation of draining tracts and distal limb subcutaneous edema on the left front and left hind limbs that had been present for 2 weeks. Direct smears of fluid collected by fine‐needle aspiration of subcutaneous fluid from both limbs were highly cellular with a predominance of eosinophils accompanied by numerous, moderately atypical, variably granulated mast cells. The cytologic diagnosis was mast cell tumor (MCT) with prominent eosinophilic infiltration with a differential diagnosis of eosinophilic granuloma. Histologic evaluation of surgical biopsies of lesions from both limbs was performed on sections stained with H&E, toluidine blue, and Luna stains. The histologic diagnosis was MCT, and staining with toluidine blue and Luna stains confirmed the presence of mast cells and eosinophils, respectively. In addition, the mast cells strongly expressed CD117. This is the first reported case of cutaneous mast cell neoplasia in a horse in which primary presenting complaints were draining tracts and distal limb subcutaneous edema involving multiple limbs. This case illustrates the utility of staining for CD117 expression in combination with traditional stains, such as toluidine blue and Luna, in differentiating MCTs from other eosinophilic lesions in horses. 相似文献
369.
Dinalva Alves Mochi Antonio Carlos MonteiroAna Carolina Ribeiro Machado Luciana Yoshida 《Veterinary parasitology》2010
The present study assessed the pathogenic effect of isolates E9, IBCB425 and IBCB159 of the Metarhizium anisopliae fungus, JAB06, JAB07 and AM09 of Beauveria bassiana, IBCB133 and CB75 of Isaria fumosorosea (=Paecilomyces fumosoroseus) and CG189 and CG195 of Isaria farinosa (=Paecilomyces farinosus) against eggs and larvae of the horn fly Haematobia irritans. Eggs were inoculated with suspensions containing 106, 107 and 108 conidia ml−1 of the fungal isolates and observed after 48 h to determine viability. In the larvae study, eggs were allowed to hatch into fresh bovine feces that had been treated with 108, 107 or 106 conidia mg feces−1. In both studies, 5 days after initial procedures, all formed pupae were transferred to an incubator at 27 ± 0.5 °C until the emergence of the adult flies was complete. The M. anisopliae isolates did not cause the death of H. irritans eggs, but they did promote the death of larvae that hatched from treated eggs, and therefore increased the total mortality. Isolate E9 promoted 100% mortality of treated larvae at a concentration of 108 conidia ml−1. For the B. bassiana isolates, no activity was observed against insect eggs or larvae. Both I. fumosorosea isolates promoted significant mortality (p < 0.05) of eggs at every concentration of conidia. Isolate CG195 of I. farinosa increased the mortality of larvae and pupae that hatched from treated eggs and promoted significant total mortality (p < 0.05) of the insect at every concentration of conidia. 相似文献
370.
Partial protection against tissue cysts formation in pigs vaccinated with crude rhoptry proteins of Toxoplasma gondii 总被引:4,自引:0,他引:4
Garcia JL Gennari SM Navarro IT Machado RZ Sinhorini IL Freire RL Marana ER Tsutsui V Contente AP Begale LP 《Veterinary parasitology》2005,129(3-4):209-217
A vaccine containing crude Toxoplasma gondii rhoptry proteins incorporated in the immunostimulating complexes (ISCOM) adjuvant was tested in pigs for protecting against tissue cyst formation. For this, 38 mixed breed pigs were divided into four groups, G1 (vaccinated challenged, n=10) received two doses (100 microg/dose) of the rhoptry vaccine at days 0 and 21, G2 (vaccinated challenged, n=10) received viable tachyzoites (7 x 10(7)) of the RH strain at day 0, G3 (unvaccinated challenged, n=10) and G4 (unvaccinated unchallenged, n=8). Pigs were challenged with 4 x 10(4) VEG strain oocysts 57 days later. The G1 pigs produced high IgG antibody levels in the indirect enzyme-linked immunosorbent assay (ELISA) after the second dose of rhoptry vaccine, but were not clinically protected against a high dose oocyst challenge. Partial protection was observed in G1 at the chronic phase of infection, when compared with G3. Pigs in group 2 developed high antibody levels and were protected against clinic signs. T. gondii was not detected in two (G1) and three (G2) pigs by mouse bioassay. The results indicate partial protection in pigs vaccinated with a rhoptry vaccine. 相似文献